Our blogs and videos choice: dna sequencing facility

DNA Test Methods - DNA Sanger Sequencing

View the full Interactive Tutorial at: www.phgfoundation.org Once a piece of DNA has been amplified, it can be sequenced. In this process, four reaction mixtures are set up, each one including: 1. DNA to be sequenced 2. DNA polymerase 3. A supply of nucleotides (A, C, G and T) 4. A small amount of a labelled chain-terminating variant of one of the four nucleotides. The enzyme DNA polymerase incorporates a chain-terminating variant at random, eventually ending the chain at every possible nucleotide position over a few hundred bases. The products of the reaction mixtures are run on an electrophoresis gel, where the sequence can be deduced by reading from the smallest to the largest piece. If different fluorescent labels are used for the variant bases, sequencing can all be done in one single reaction, the bands can be detected and the sequence read out automatically. The entire process of DNA sample preparation and sequencing is now highly automated, a development that has been essential for the timely and cost-effective completion of the human genome project. [ go to Youtube.com ]

Time : 1 min 08
Added : 08/10/08 10:29

Videos (23) | Blog posts (22)
 

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Foundations in DNA Sequencing

DNA sequencing technologies accelerated our understanding of genome structures, as well as enabled recombinant DNA technology. This presentation describes the two fundamental technologies used to sequence DNA, devised in the late 1970's. This presentation is the YouTube component of a graduate journal colloquium in Transformative Advances in Molecular Biology at the University of Florida. The course is part of the Horticultural Sciences Department and the Graduate Program in Plant Molecular and Cellular Biology and was led by Dr. Mark Settles and Dr. Kevin Folta [ go to Youtube.com ]

Time : 7 min 58
Added : 11/12/09 19:12

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Nanotechnology - Sequencing DNA

This is a video I made for a nanotechnology class back in the spring semester of 2008. I've noticed that that recently, more news is being reported about the technology known as nanosequencing, especially nanopores, so I thought I'd put this out there for anyone who might want to know about it a little more. This is the first video I've posted that wasn't recorded on my phone. I used iMovie and CC photos (for the most part) so please don't expect a michael bay/steven spielberg production. [ go to Youtube.com ]

Time : 8 min 31
Added : 01/03/09 22:11

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TO THE CONTRARY | Meet the Beerys: DNA Sequencing and Rare Childhood Diseases | PBS

In the conclusion of our three-part series on genetic mapping and rare pediatric diseases, the Beery family shares their story of heartbreak and hope. Make sure to tune into this week's edition of To The Contrary on your local PBS station (check local listings). For more information visit our website! www.pbs.org [ go to Youtube.com ]

Time : 7 min 18
Added : 30/07/10 22:04

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Oxford Nanopore: BASE (TM) DNA Sequencing with voiceover

Oxford Nanopore Technologies is developing a new generation of DNA sequencing technology that does not require fluorescent labels or optical instrumentation, identifying the series of bases directly using electronics. This animation shows the BASE sequencing process. For further information visit www.nanoporetech.com. [ go to Youtube.com ]

Time : 1 min 32
Added : 10/01/09 13:00

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Oxford Nanopore Technologies: BASE (TM) DNA sequencing

Oxford Nanopore Technologies is developing a new generation of DNA sequencing technology that does not require fluorescent labels or optical instrumentation, identifying the series of bases directly using electronics. This animation shows the BASE sequencing process. For further information visit www.nanoporetech.com. [ go to Youtube.com ]

Time : 2 min 19
Added : 06/01/09 11:16

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NABsys DNA Sequencing

This technology may reduce the cost of whole-genome DNA sequencing by more than four orders of magnitude. This will allow for the elucidation of the genetic components of complex disease states and ultimately for the adoption of personalized and preventive medicine. [ go to Youtube.com ]

Time : 3 min 12
Added : 30/04/09 18:02

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Applied Biosystems 3500 Series Genetic Analyzers for DNA Sequencing and Fragment Sizing

The 3500 Series Genetic Analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative consumables system approach. To learn more, please visit: marketing.appliedbiosystems.com [ go to Youtube.com ]

Time : 8 min 39
Added : 17/02/10 21:44

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DNA Sequencing

ncbionetwork.org The "Experiments in Biotechnology" video series is a production of NCCCS BioNetwork in partnership with Haywood and Western Piedmont Community Colleges and features demonstrations of 8 laboratory experiments. [ go to Youtube.com ]

Time : 5 min 41
Added : 13/05/10 17:44

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Hate Brussels Sprouts? It's Genetic - Pearl Biotech's Open PCR Project

If you hate the taste of Brussels sprouts it's likely genetic and now you can prove it to your mom and dad with the Pro Biotech's open source PCR project. Gene sequencing at home. biocurious.org Filmed at Maker Faire California 2010 [ go to Youtube.com ]

Time : 9 min 36
Added : 26/05/10 17:40

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