To obtain these structures, a new, efficient solid-phase synthesis protocol was developed as well. To determine the binding affinity and selectivity of these compounds among all possible 4-base pair sequences of DNA, a high throughput (HT), fluorescence intercalator displacement (FID) assay was employed. The FID results indicate that these compounds are good DNA minor groove binders as predicted (more than 50% fluorescence decrease at 10 μM for A/T only sequences); the amidine group improves the binding affinity and selectivity compared to amino acid-benzimidazole conjugates without an [more...]
Date: 2010-08-25 22:40:17
Development of a Novel Fluorescence Assay Based on the Use of the ...
Human -alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the position of guanine. ... Oligonucleotide synthesis was performed by the solid-phase - cyanoethyl-phosphoramidite method . Ammonia treatment was performed at room temperature overnight to minimize decomposition of O6-methylguanine. For the same reason, the dimethylformamidino group was selected for the protection of [more...]
Date: 2010-07-17 07:00:00
DNA Synthesis and Repair Genes RRM1 and ERCC1 in Lung Cancer — NEJM
Original Article from The New England Journal of Medicine — DNA Synthesis and Repair Genes RRM1 and ERCC1 in Lung Cancer. ... of gemcitabine and platinum in non-small-cell lung cancer. J Clin Oncol 2006;24:4731-4737 CrossRef | Web of Science | Medline. 18. Sandler AB , Nemunaitis J , Denham C , et al. Phase III trial of gemcitabine plus cisplatin versus cisplatin alone in patients with locally advanced or metastatic non-small-cell lung cancer. J Clin Oncol 2000;18:122- 130 [more...]
Date: 2007-02-22 05:00:00
Alnylam Scientists Present New Data on RNAi Therapeutics at ...
Further, several of the presentations describe the design, synthesis, and analytical characterization of siRNAs with optimized properties, including: “Solid-phase synthesis of 5'-di- and tri-phosphates and their modified analogs of DNA, RNA and chemically modified oligonucleotides,” by Ivan Zlatev, Ph.D., Scientist; “Parallel high throughput synthesis of chemically modified 21-27mer siRNA sequences,” by Satya Kuchimanchi, Ph.D., Associate Director, Small Scale Synthesis; “Novel method for the confirmation of siRNA sequence by LC-MS/MS,” by Gary Lavine, Ph.D., Senior [more...]
Date: 2010-08-24 12:13:50