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DNA sequencing » Blog Archive » DNA Sequencing Methods

DNA Sequencing – How DNA Sequencing Works – Methods for … An explanation of how DNA sequencing works. … Typically, the automated sequencing method is only accurate for sequences up to a maximum of about 700-800 base-pairs in length. … DNA Sequencing Simulation (Sanger Method) Within each reaction mixture are the following items: a DNA segment of unknown … The other DNA sequencing method (named for Allan Maxam and Walter … Next-Generation DNA Sequencing Methods 921k – Adobe PDF – View as html generation DNA sequencing technologies highlight the striking impact … [more...]

Date: 2010-09-28 04:29:58

Blog posts (25) | Videos (22)

Sequenziamento del DNA- Prima generazione (metodo Sanger) « my GenomiX

Così, ecco la prima parte della mia serie sul DNA sequencing: oggi tratterò la prima generazione, vale a dire il metodo Sanger. Innanzitutto facciamo un piccolo ripasso sul DNA. Il DNA (o acido deossiribonucleico) è una molecola che ha l’aspetto di una doppia elica formata da due catene di nucleotidi. I nucleotidi sono piccole molecole costituite da un gruppo fosfato, uno zucchero (deossiribosio) e una base azotata; mentre le prime due componenti sono sempre uguali e costituiscono l’ossatura della doppia elica, le basi azotate esistono in quattro “versioni” differenti. Esse si [more...]

Date: 2010-09-08 08:31:06

Genetic alterations common to Kabuki syndrome discovered through ...

Second-generation sequencing technologies have some difficulty identifying some types of genetic variants that lead to a loss of function in genes. Follow-up sequencing using the traditional Sanger DNA sequencing method, [more...]

Date: 2010-08-15 18:15:00

Antibody Therapies and the New Generation of DNA Sequencing ...

Our antibody database targets protein-coding genes. Although they form only 1% of the total human genome, they account for 85% of the mutations that lead to disease. DNA sequencing (defining the sequence of the 4 base amino acids within the DNA strand) and then using antibodies specific to those sequences, is a vital part of disease research. Sanger sequencing is the standard for doing this. However, in 2007 a next generation (or next gen) sequencing technique was devised. Lauded as the biggest advancement in genomics since microarray technology, it has radically improved diagnostic [more...]

Date: 2010-09-27 10:34:46

Basics: Sequencing DNA, Part 1 « Genetic Inference

However, in certain respects Sanger Sequencing is still top-of-the-range. The 4-channel capillary approach lets you sequence DNA pretty fast, and each machine can often do hundreds of reactions at once. It was these machines that first sequenced the human genome. In addition, you can sequence relatively long sequences of DNA; up to 1000 nucleotides (after that, it gets difficult to tell the difference between molecules of different sizes). We have yet to make a machine that can sequence DNA fragments of this length faster than Sanger Sequencing (yet…). The modern Sanger Sequencing machines [more...]

Date: 2009-04-17 21:31:39


Research work conducted here is aimed at understanding the role of DNA sequence variation in human health and disease.Scigenom's sequencing in India provides Sanger sequencing, analysis and genotyping service to research institutions, investigators and laboratories worldwide. [more...]

Date: 2010-09-21 10:52:32

Scientist Solutions - The Sanger DNA Sequencing Method

Would you like to save this topic, event, protocol or job so you can find it again easily? Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space. Available to members only. Please log in or register for your free account [more...]

Date: 2009-02-04 18:51:55

Halfbakery: Ligase sequencing

Currently the standard method (Sanger sequencing, or di-deoxy sequencing, or dye-terminator sequencing) is to purify the DNA, then transcribe a section from one particular point in the presence of fluoresently labelled chain terminators [more...]

Date: 2005-08-05 00:00:00

The Evilutionary Biologist: This Week's Citation Classic

Definitely, the Sanger method started a new epoch. I'm wondering if now we are living the end of the Sanger era. The DNA sequencing technology from 454 Life Sciences seems to be expanding quite fast. If the technology is as good as they say, it might bury the Sanger method into ancient history. Sunday, May 13, 2007 7:11:00 AM [more...]

Date: 2007-05-11 02:56:00

DNA Sequencing with Reversible dNTP and Cleavable Fluore

In the grant application, we will pursue the development of a DNA sequencing system that is a hybrid between Sanger dideoxy chain terminating reaction and SBS using molecular engineering approaches. In this approach, four nucleotides, [more...]

Date: 2008-08-19 17:00:00

inqaba biotec and BODE Technology to host 2nd Annual African DNA ...

Oligonucleotide synthesis; Sanger sequencing service using the ABI 3130XL and ABI 3500XL sequencers; High-throughput DNA sequencing service using a Roche /454 GS FLX sequencer; Customised services like amplicon cloning, [more...]

Date: 2010-03-01 07:59:59

Whole-Genome Sequencing in a Patient with Charcot–Marie–Tooth ...

DNA-sequencing methods that involve chemical assays other than the traditional Sanger dideoxy-chain-termination method. Next-generation-sequencing methods produce much larger quantities of data at less expense, but the individual raw [more...]

Date: 2010-03-31 20:55:45

Biology Animations: DNA Sanger Sequencing Animation

Biology Animations Website includes selected, high quality biological animations. You can find information about cell biology, microbiology, genetics, [more...]

Date: 2010-05-07 17:21:00

Nobel season '10: Chemistry

I've also seen that different large-scale DNA-sequencing approaches, primarily shotgun sequencing, are on the map in Swedish media which I think is really strange. Firstly because Fred Sanger, who developed shotgun sequencing, is 92 years old and a double Nobel laureate in chemistry already (1958 and 1980), and secondly because the field of large-scale sequencing still hasn't peaked by any means. Perhaps Leroy Hood (again) could be considered for the development of the first automated DNA sequencer? Anyway, the technologies that are advancing the field have been developed by private companies [more...]

Date: 2010-10-06 07:00:00

Fungal Molecular Diagnostics

(ie, the Maxam-Gilbert chemical cleavage method71 and the Sanger chain-terminator method72) have been reviewed in detail in previous publica- tions.71–73 When attempting to identify microorgan- isms using DNA sequencing, the gene [more...]

Date: 2008-04-05 07:00:00

DNA Sequencing Videos,Photos,Informations,Details,Collections ...

DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA. The most popular method for doing this is called the dideoxy method or Sanger method (named after its inventor, Frederick Sanger, who was awarded the 1980 Nobel prize in chemistry for this achievment). DNA is synthesized from four deoxynucleotide triphosphates. The top formula shows one of them: deoxythymidine triphosphate (dTTP). Each new nucleotide is added to the 3′ -OH group of the last nucleotide added. Link to discussion of DNA synthesis. The dideoxy method gets its name from [more...]

Date: 2010-08-25 06:59:00

Sandwalk: Nobel Laureate: Walter Gilbert

The Nobel Prize in Chemistry 1980. "for their contributions concerning the determination of base sequences in nucleic acids" Walter Gilbert (1932 - ) was awarded the Nobel Prize for developing a chemical method of sequencing DNA (with Allan Maxam). The method relied on chemical reactions that cleaved DNA at specific residues. By carrying out partial reactions where only one cleavage occurred in each DNA strand, it was possible to separate the cleavage products on an acrylamide gel and determine the position of each residue by the length of the fragment. The chemical sequencing strategy has [more...]

Date: 2008-12-10 14:35:00

Science News for Kids: Feature: Scientist Profile: Wally Gilbert

You've won a Lasker Award and a Nobel Prize for your research on sequencing DNA, basically figuring out the order of the letters in this very long word. How does that method work? Fred Sanger in England invented one method, and I invented another with my collaborator, Allan Maxam. The very first piece of DNA we deciphered was only 20 groups long. It took us two years to work out the order of those groups. A typical gene in our body is some ten thousand groups long, so you couldn't possibly work out the entire order of groups for a gene. Then suddenly we discovered a simple way of doing it. It [more...]

Date: 2010-03-31 04:00:00

Bioinformatics Programmers Require For Scigenom Labs Pvt Ltd /M.Sc ...

We conduct research work aimed at understanding the role of DNA sequence variation in human health and disease. Our state of the art DNA sequencing laboratory providing Sanger sequencing, analysis and genotyping service to research institutions, investigators and laboratories worldwide. Name of the Position : Bioinformatics Programmers Number of Opening : one Name of the Institute : Scigenom Labs Pvt Ltd Functional Area :Application Programming, Maintenance Experience: 1 - 5 Years Location:Ernakulam / Kochi/ Cochin Desired Candidate Profile : The person in this position should have good [more...]

Date: 2010-08-31 18:47:29

Genomics: The next generation | Wellcome Trust

The advent of the Human Genome Project and the development of faster, cheaper DNA sequencing revolutionised medical and biological research - and the technology is still evolving. Mun-Keat Looi looks at the platforms pushing us closer toward the ‘$1000 genome’. At the turn of the millennium, genomics captured the public's imagination with the publication of the first draft of the human genome and the insights it offered into our biology. When the Human Genome Project started in 1991, DNA sequencing entailed laborious radiation-based methods, with researchers manually loading [more...]

Date: 2009-07-28 11:00:00

Mutation Rate In Humans Gets Measured By Direct Sequencing

With better measurements of mutation rates, we could improve the calibration of the evolutionary clock, or test ways to reduce mutations, for example. Even with the latest DNA sequencing technology, the researchers had to design a special strategy to search for the vanishingly rare mutations. They used next-generation sequencing to establish the order of letters on the two Y chromosomes and then compared these to the Y chromosome reference sequence. Having identified 23 candidate SNPs – or single letter changes in the DNA – they amplified the regions containing these candidates and [more...]

Date: 2009-08-27 14:01:30

dna sequencing

DNA sequencing reactions all use a primer to initiate DNA synthesis.  A Quicktime animation of how dideoxy nucleotides are incorporated into DNA in sequencing reactions. Nucleic acid sequence This sequence is written as a succession of letters representing a real or hypothetical DNA molecule or Sequences can be read from the biological raw material through DNA . DNA Sequencing – Real Time DNA analysis Information With the latest chellenges in DNA Sequencing, our organization looks to unravel the myths, truths and most essential assets in the deconstruction of genes. DNA Sequencing The [more...]

Date: 2010-07-09 03:20:29

Next Generation Sequencing - Biotechnologies

For the first time in nearly a decade there is a new choice in commercially available DNA-sequencing platforms. As a result there has been a flurry of development activity that promises to lead to other new platforms being generally available. This has energized the sequencing community and, more than ever before, encouraged new entrants into the field. The achievement of the human genome project was entirely performed by fluorescent Sanger di-deoxyribonucleotide sequencing, which was almost exclusively provided by Applied Biosystems. Other vendors also supported fluorescent/Sanger-based [more...]

Date: 2006-10-25 14:53:00

FinchTalk: From Reads to Data Sets, Why Next Gen is not like ...

The first widely-used sequencing systems were based on the “Sanger” method. DNA was synthesized in the presence of chain terminating radioactive dideoxy-nucleotides (1). Mixtures of DNA fragments were separated by size using gel electrophoresis and the bases were identified and entered into a computer through manual techniques. Automated DNA sequencing instruments arrived later. These instruments made DNA sequencing thousands of times more efficient by detecting fluorescently labeled fragments and sending the information directly to a computer (2). For the first time, it became possible [more...]

Date: 2009-01-06 19:16:00

Human DNA : Surprisingly Diverse

Human DNA : Surprisingly Diverse Agençe France-Presse 23 November 2006 Source: www.abc.net.au New investigations into the code for life suggest the assumption that humans are genetically almost identical is wide off the mark, and the implications could be resounding. Current thinking, inspired by the results five years ago from the Human Genome Project, is that the 6 billion humans alive today are 99.9% similar when it comes to genetic content and identity. But research published today in the journal Nature suggests we are genetically more diverse. The repercussions could be far-reaching [more...]

Date: 2010-10-08 11:11:33

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