Our blogs and videos choice: pcr dna

Forensic Serology Information Page

What is Forensic Serology?According to forensic serologist Marcella Jones of www.ForensicMentor.com, forensic serology is the analysis of body fluids as they relate to forensic cases, including DNA analysis. Accordingly, the role of the forensic serologist involves:Examining evidence for the presence of body fluids e.g. blood, semen, hair, tissue, saliva, feces, and urineEvaluating evidence for potential DNA analysisEvaluating species of body fluidsPerform PCR (polymerase chain reaction) based typing of STR (short tandem repeat sequences) of genomic DNA, or mitochondrial sequence analysis of [more...]

Date: 2010-02-04 08:00:00

Blog posts (144) | Videos (26)

DNA fingerprinting simplified

One of the most frequently used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic technique often used in genetic or DNA fingerprinting. The procedure is achieved by moving negatively charged nucleic acid molecules through a gelatinous substance known as agarose by using an electric field. Andrew, in collaboration with his father and other researchers, completed the study, which is published in the January 2008 issue the American Society of Horticultural Science’s journal HortTechnology. The youngest author ever to publish in an ASHS journal, Andrew [more...]

Date: 2008-05-14 04:52:12

Variants of PCR (1)

The bacteriophage T4 DNA polymerase was also tested shortly after the first reports of PCR. It has a higher fidelity of replication than the Klenow fragment. Since it is also destroyed by heat, it has seen little use since the development of thermostable polymerases. The DNA polymerase from Thermus aquaticus (or Taq), was the first thermostable polymerase used in PCR, and is still the one most commonly used. The enzyme can be isolated from its 'native' bacterial source, or from a cloned gene expressed in E. coli. The Stoffel fragment is produced from a truncated gene for Taq polymerase, [more...]

Date: 2008-09-11 03:56:00

Is there a different type of primer i have to use when running PCR ...

Question by bob oso: Is there a different type of primer i have to use when running PCR with genomic DNA compared to cDNA? Im still in school but when i run a PCR, I normally isolate RNA first then RT to cDNA and run PCR with that. This time i isolated the genomic DNA from the sample and ran PCR using the beta-actin primer i always used. Dimers were faintly visible on my gel but nothing really came up like it normally would have or should have. I was told that when running PCR with genomic DNA, a different kind of primer had to be used; one that is specific to genomic DNA. Im confused as to [more...]

Date: 2010-10-01 19:54:46

HTB – Early infant diagnosis

She concluded that promising POC assays for EID today include: IQuum’s LIAT, SAMBA, CIGHT p24 and possibly CLONDIG’s viral load assay. comment This was an incredibly useful overview and it looks like we can be optimistic about having a POC test for EID in the next couple of years. Other presentations at the conference dealt with the challenges of access to EID. In the same session, Shaffiq Essajee noted that EID access has improved and in some countries more than 50% of exposed infants are tested. But infant testing is usually linked to PMTCT and if coverage is low, so is infant [more...]

Date: 2010-09-30 06:04:59

An ELISA-based high throughput protein truncation test for ...

Here, we report the next generation HTS-PTT using triple-epitope-tagged proteins and demonstrate, for the first time, its efficacy on clinical genomic DNA samples for BRCA1/2 analysis. Methods: Segments of exons 11 of BRCA1/2 open reading frames were PCR amplified from either blood derived genomic DNA or cell line mRNA. PCR primers incorporate elements for cell-free transcription/translation and epitope tagging. Cell-free expressed nascent proteins are then antibody-captured onto the wells of a microtiter plate and the relative amount of truncated polypeptide measured using antibodies against [more...]

Date: 2010-10-04 16:47:13

ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...

Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]

Date: 2010-08-25 00:51:13

DNA: Size Does Matter. But is it Science or Art? « Exploratorium ...

Then I randomly found this website, which starts with the same idea as our DNA Cheek Cell Extraction Demo but takes it to a whole new level:  You send your cheek cells in the mail to their company.  They extract your DNA from your cells, then use PCR to amplify unique sections of DNA in your genome.  This DNA is loaded into a gel and a current is run through the gel to separate the DNA based on size.  UV dye is added to the whole thing to make your DNA strands visible, a photograph is taken, and voila!…a giant canvas print with the color scheme of your choice is ready to hang on your [more...]

Date: 2008-05-14 07:37:47

E. Coli Plasmid Vectors: Methods and Applications (Methods in ...

E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) (archive) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, N.J.), V. 235.) Table of Contents The Function and Organization of Plasmids Finbarr Hayes Choosing a Cloning Vector Andrew Preston Escherichia coli Host Strains Nicola Casali Chemical Transformation of E. coli W. Edward Swords Electroporation of E. coli Claire A. Woodall DNA Transfer by Bacterial Conjugation [more...]

Date: 2004-04-04 08:00:00


SSR assays require a minimal amount of genomic DNA. Most of the crop improvement programs are confined to evaluation and selection of naturally occurring clonal variations. In vitro culture techniques provide an alternative means of plant propagation and an important tool for crop improvement. The in vitro cultures are also of low risk for genetic variation since it is more resistant to genetic changes while occurrence of cell division in vitro condition. Gel electrophoresis is an important molecular biology tool which enables us to study DNA. It can be used to determine the sequence of [more...]

Date: 2010-09-08 04:07:38

DNA sequencing » Blog Archive » Real Time PCR

Real Time PCR Primer Sets Validated primer sets for quantitative Real Time PCR – Only $24.95. Real-time polymerase chain reaction – Wikipedia, the free Real time quantitative PCR uses fluorophores in order to detect levels of gene expression. Real-time PCR can also be applied to the detection and quantification Real Time PCR Tutorial In real-time PCR, we usually use a reverse transcriptase that has an endo H activity. So how do we use real-time PCR to quantitate the amount of DNA or cDNA? Real Time PCR Expertise in dye chemistries and oligonucleotide labeling has allowed Invitrogen to [more...]

Date: 2010-08-13 02:02:13

- “Paternity Testing”

Paternity testing results? - Yahoo! UK & Ireland Answers Does anybody know how reliable dna testing was in 1982?, could it have … Seriously i think you would be better to ask a Medical Doctor, rather then random ppl … uk.answers.yahoo.com/question/index?qid… Advanced Testing Center - 10 Commercial St , Foxboro, MA 02035 … … igm test pcr test hiv rna test igg testing igm testing pcr testing dna test dna testing confidential herpes testing paternity test paternity testing. … www.getfave.com/12341786-advanced-testing-center court- ordered paternity testing in michigan? how would [more...]

Date: 2010-04-29 23:36:17

X-Tagged.com Adult Social Bookmarking - DNA sequencing

PCR Analysis facilitates the capacity to make millions of DNA copies from a single biological sample. This type of analysis enables the forensic scientist to examine small data such as a few skin cells. It also lessens the chances of contamination of the sample, but it requires very meticulous work. Short tandem repeat (STR) technology Short tandem repeat (STR) technology analyzes all 13 Loci (regions )for DNA testing and is the technology used by the FBI databases at the local, state, and federal level. Short tandem repeat (STR) technology for DNA profiles from convicted offenders, unsolved [more...]

Date: 2010-10-12 18:10:35

Eppendorf to market Akonni TruTip™ « Frederick County Biotech ...

HAUPPAUGE, N.Y. & FREDERICK, Md.–(BUSINESS WIRE)–Eppendorf North America and Akonni Biosystems today announced they have entered into a joint marketing agreement to promote Akonni TruTip nucleic acid extraction kits configured for use with Eppendorf epMotion automated pipetting systems. Under the agreement, Eppendorf will promote the extraction kits to clinical, clinical research and forensic laboratories in North America – providing users with access to the industry’s most rapid and reliable means to automatically extract PCR-ready DNA and/or RNA from larger volume [more...]

Date: 2010-04-26 16:36:03

Methylation Test May Aid Colon Cancer Diagnosis (CME/CE) | Discuss ...

To develop a more sensitive test for DNA methylation, investigators employed a form of technology known as BEAMing (beads, emulsion, amplification, and magnetics). They performed PCR amplification of individual DNA fragments covalently linked to magnetic beads suspended in water-based nanoparticles immersed in oil droplets. The beads contained a DNA sequence specific for exon 1 of the vimentin gene, which is hypermethylated in colon cancer. Following PCR amplification, the beads were hybridized by means of fluorescent probes specific to the state of methylation and then sorted and analyzed by [more...]

Date: 2010-10-09 11:00:27

PhD Student for Ecology and Ecological Epigenetics in Plants ...

Recently however, evidence is accumulating that epigenetic processes, such as DNA methylation and chromatin modification, may play an important role in the short-term adaptation of plants to rapidly changing environmentats, such as are the consequence of climate change.In a European wide project (EpiCOL), considering the role of epigenetic regulation mechanisms as an alternative means towards adaptation in plants, we work on delivering “proof of principle” for the ecological and evolutionary significance of epigenetic variation. The project is a cooperation between ecologists, [more...]

Date: 2010-10-13 03:56:15

Veg Physiology Research Associate Post vacant at Monsanto, Saint ...

Perform a variety of laboratory functions including preparation of media and buffers,, DNA extraction, PCR and other duties as assigned. Interact with a variety of customers and support groups including research, breeding, sales, inside farming, facilities, outside farming. Actively participate in ESH (Environment, Safety & Health) programs on site Qualifications M.Sc in Crop Science (preferably) or Biotechnology; with 2+ years professional research experience in Breeding. Experience with vegetable crop species. Computer proficiency, particularly in MS Office: MS Word, MS Excel, MS [more...]

Date: 2006-04-05 07:00:00

Antigen/Antibody Discovery Research Scientist (Sorrento Mesa, San ...

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! CTK Biotech Inc, a leading IVD biotech company located in San Diego area, is seeking an Antigen/Antibody Discovery Research Scientist to join a group of scientists and technicians in the R&D Department. The current position will play a major role in researching and discovering high quality and specific diagnostic reagents to meet the rapidly growing demand of the point-of-care IVD products worldwide. Essential Duties and Responsibilities: Note: Other duties may be assigned. •Perform cloning and [more...]

Date: 2010-10-12 03:53:43

DNA sequencing » Blog Archive » DNA Sequencing Gel

Preparation of DNA Sequencing Gel 3. Assemble the gel rig on the sequencer, being careful not to get … 7. Run the gel for 45 minutes. E. Denature the sequencing reactions and prepare them for … DNA sequencing gel Hahn Lab Methods DNA sequencing gel. Acrylamide Urea Gel (100 ml) … if the top of the gel is wet with water and is wrapped tightly in Saran Wrap … How do we Sequence DNA? DNA Sequencing is at the center of the Human Genome Project, which promises to … sequencing gel: That’s exactly what we do to sequence DNA, then … Chapter 8 A. Recombinant DNA Technology PCR has [more...]

Date: 2010-09-28 04:49:52

The Quality Stocks Stock Newsletter For Smallcap Companies Blog ...

Shivji noted the 15 years of genomic research and leadership experience of Dr. Schroth, and his tireless efforts to advance gene expression techniques using DNA sequencing, microarrays, PCR and next-gen sequencing, all of which has resulted in numerous publications and patents spanning the entire field of genomic analysis. Dr. Schroth expressed his excitement over the opportunity to push the SmartChip platform forward as a perfect solution for multiple global research efforts that are focused on making improved and more targeted therapeutics, and called the SmartChip system “the most [more...]

Date: 2010-10-12 17:57:42

Research Technician II (Durham) | PCR Jobs

Three years of molecular biology research and experience in the following is preferred: DNA/RNA extraction; DNA sequencing; PCR/ Real Time PCR; Taqman; gel electrophoresis; and tissue culture (propagation and storage). Computer proficiency in MS office programs, Photoshop, and basic statistical programs is also preferred. Qualified candidates should email a resume to the “reply to” email above. Please note “Tech II” in the subject line. Duke University is an Equal Opportunity/Affirmative Action Employer. Topics: Raleigh-Durham PCR jobs | No Comments [more...]

Date: 2010-10-11 18:43:40

Make: Online : Garage biotech in <em>Nature</em> magazine

And several groups are focused on making standard instruments -- such as PCR machines, which amplify segments of DNA -- cheaper and easier to use outside the confines of a laboratory, ultimately promising to make DIYbio more accessible. Garage biotech: Life hackers Editorial: Garage Biotech Disclosure: The article features a sexy picture of my own project, OpenPCR, a kit to PCR DNA. Bio: MAKE guest citizen science author Tito Jankowski works on making biotech easier to do, including developing open source tools for gel electrophoresis and PCR. Got other citizen science or garage biotech [more...]

Date: 2010-10-12 00:00:00


GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are  involved in various disease problems in humans and aquatic  animals and are known to be  phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific  diagnostic test is warranted for detection and characterization of this pathogen.In the present study,  Fish  and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]

Date: 2010-10-08 20:08:05

PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading

Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]

Date: 2009-11-13 08:00:00

Cytogenetics Clinical Lab Technologist Job / Mayo Clinic ...

Knowledge of genetics, molecular genetics, DNA extraction, PCR, microscopy, cell culture and aseptic technique is desirable. Experience with operation and troubleshooting of laboratory instrumentation and assay systems is desirable. CV/RESUME, COVER LETTER, and TRANSCRIPTS, unofficial copy accepted, MUST ALL BE INCLUDED FOR YOUR APPLICATION TO BE CONSIDERED. **A 2 YEAR COMMITMENT TO THIS POSITION IS REQUIRED. Benefit Eligibility: Yes Posting Begin Date: 08/06/2010 Posting End Date: Until Filled Employment Type: Non Exempt Hours/2-Weeks: 80 Schedule: Monday - Thursday Shift: [more...]

Date: 2010-10-09 21:08:40

Basics: Sequencing DNA, Part 1 « Genetic Inference

However, in certain respects Sanger Sequencing is still top-of-the-range. The 4-channel capillary approach lets you sequence DNA pretty fast, and each machine can often do hundreds of reactions at once. It was these machines that first sequenced the human genome. In addition, you can sequence relatively long sequences of DNA; up to 1000 nucleotides (after that, it gets difficult to tell the difference between molecules of different sizes). We have yet to make a machine that can sequence DNA fragments of this length faster than Sanger Sequencing (yet…). The modern Sanger Sequencing machines [more...]

Date: 2009-04-17 21:31:39

Research Scientist

Research Scientist Sep 14, 2010 Posting date: April 2010 Description: Seeking energetic and meticulous Research Scientist to lead a team in developing DNA purification protocols and real-time PCR assays to support Spartan’s real-time DNA analyzer. Responsibilities will include collaborating with industry and research partners, managing technicians, and writing technical notes and publications. Microbiology, virology and experience in food safety an asset. Qualifications: Ph.D. in Biological Sciences Peer-reviewed publications Extensive experience with molecular biology techniques, [more...]

Date: 2010-09-14 22:04:55

呼吸器内科医 : CAPの患者ではウイルス検出率が健常人より高い

この検体は12の呼吸器ウイルスをmultiplex TaqMan Hydrolysis probeを用いた  リアルタイムPCR(Integrated DNA Technology; Coralville, IA)で解析した。 結果:  少なくとも、1つの呼吸器ウイルスが58人のCAP患者から検出(31.7%)された。  コントロール群では32人 (7.1%)、NPLRTI群では104人(51.7%)。  (それぞれP< .01 、P< .01)  コロナウイルスはCAP患者の24人(13.1%)、コントロール群の17人(3.8%)、  NPLRTI群の21人(10.4%)で認められた。  RSウイルスはCAP群の13 [more...]

Date: 2010-10-06 05:31:42

Research Technician

Research Technician Sep 14, 2010 Posting date: April 2010 Description: Seeking energetic and meticulous Research Technician to develop DNA purification protocols and real-time PCR assays for Spartan’s DNA analyzers. Responsibilities will include designing, performing, and analyzing experiments. Qualifications: B.Sc. in Biological Sciences Experience with molecular biology techniques, preferably including real-time PCR Strong interpersonal and communication skills To apply, please forward your resume to [more...]

Date: 2010-09-14 22:08:03

Bio Saga Blog: 5-Day Hands-on Workshop on Molecular Biotechnology ...

Recombinant DNA Technology: Cutting & pasting of DNA molecules (i.e. restriction digestion, ligation, DNA gel eectrophoresis, and gel extraction.) Bioinfo part: Bioinformatics of DNA database / SequenceAnalysis: Pairwise sequence alignment, Multiple sequence alignment, Pattern search.Tools: BLAST, CLUSTALW/X, CLC Bio Main Workbench Genetic Engineering: Transformation and plasmid purification, cloning and sub-cloning, amplification of DNA fragments by polymerase chain reaction (PCR). Bioinfo part: Designing of PCR primers In-silico cloning Searching for homologous and paralogous [more...]

Date: 2010-09-29 05:41:00

Genome Biology | Full text | Optimization and clinical validation ...

Novel features of this platform include the development of a robust algorithm that accurately predicts PCR bias during DNA amplification and can be used to improve PCR primer design, as well as a powerful statistical concept for inferring .... This probability is reduced when intra-primer hairpin formation is predicted, and increased according to degree of complementarity between tag sequence and viral sequence. In this manner, the probability that each nucleotide will be [more...]

Date: 2007-05-27 00:00:00

Part-time Lab Assistant-Tissue culture (Sorrento Valley)

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Sorrento Valley biotech start-up seeks a part-time Lab Assistant to be responsible for mammalian cell culture / cell line generation. Work hours: 15-25 hr/week Job duties: • Stable mammalian cell line generation • Mammalian suspension cell culture • Expression vector cloning including transformation, ligation, DNA gel extraction, and mini/midi prep • Protein purification and analysis work as needed • General lab maintenance Requirements: • BA with 2-3 years of experience • Strong [more...]

Date: 2010-04-02 03:24:50

How Fingerprinting Helps In DNA Paternity Testing

This form of fingerprinting method is also known as DNA profiling. Using this technique, the individuals are identified on the basis of their unique DNA profiles. This DNA profiling is done by analyzing the VNTR sequences. It stands for variable number tandem repeats. This sequence is found to be different in individuals except in twins. As per the facts mentioned above, the DNA samples of the child will exhibit the similar VNTR loci as that of their parents. This fingerprinting helps in identifying that whether the known father of a child is genuinely his biological dad. In fact, it is [more...]

Date: 2010-08-09 11:41:10

Case Analyst (Salt Lake City)

Successful candidate will perform genotyping analysis. Experience using GeneMapper genotyping software a plus. Experience performing DNA extraction, PCR and genotyping for STR analysis and mitochondrial sequencing essential. Other lab duties as directed. Required: • BS in Biochemistry, Molecular Biology, Biology or related field • 1 year experience in laboratory • Knowledge of molecular biology testing, analysis and reporting. • Must be able to use hands, stand for periods of time and lift up to 20 lbs. Preferred: • Computer and Microsoft applications proficiency • 2 years [more...]

Date: 2010-10-01 04:43:12

HTB – Low birth weight associated with HAART in pregnancy in Zambia

At the time of the analysis, 200 women had delivered 206 live infants and six week HIV DNA PCR results were available for 158 infants, out of which two (1.3%) were diagnosed with HIV. The combined rate of HIV-infection at six weeks and mortality at three months post partum was 8/153 (5.2%) compared to 144/958 (5.2%) in ZEBS, p<0.001. They reported a mean birth weight for the Aluvia cohort of 2.9kg (SD 0.5) with a LBW incidence of 35/206 (16.9%) compared to 105/937 (11.2%) in ZEBS, p=0.02. There were no significant differences in maternal risk factors for LBW (CD4 <350 cells/mm3, anaemia, [more...]

Date: 2010-09-30 15:42:40

"Polymerase Chain Reaction (PCR)" Biology Animation Library ...

"Polymerase Chain Reaction (PCR)" Biology Animation Library :: Dolan DNA Learning Center: "Polymerase Chain Reaction Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA. This animation is featured in our 'Spotlight Collection' on Polymerase Chain Reaction, along with video interviews with Kary Mullis, a 3D molecular animation of PCR, and several laboratory [more...]

Date: 2010-08-23 17:54:00

Predictors of global methylation levels in blood DNA of healthy ...

Background Estimates of global DNA methylation from repetitive DNA elements, such as Alu and LINE-1, have been increasingly used in epidemiological investigations because of their relative low-cost, high-throughput and quantitative results. Nevertheless, determinants of these methylation measures in healthy individuals are still largely unknown. The aim of this study was to examine whether age, gender, smoking habits, alcohol drinking and body mass index (BMI) are associated with Alu or LINE-1 methylation levels in blood leucocyte DNA of healthy individuals. Methods Individual data from five [more...]

Date: 2010-09-16 05:22:51

Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...

Genetic Fingerprinting of Bacillus thuringiensis Isolates by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)(Published in Nepal Journal of Science and Technology 10(2009) 97-103)Gyan Sundar Sahukhall, Upendra Thapa Shrestha1, Binod Lekhak2, Anjana Singh2, Viswanath Prasad Agrawal11Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal. 2Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepale-mail: gyan633413@gmail.com AbstractRandom Amplified Polymorphic DNA (RAPD) is a method of producing a genetic fingerprint of a [more...]

Date: 2010-01-21 14:06:00

Technology Review: A Portable DNA Detector

A new portable DNA analyzer performs real-time analysis of blood samples left at the scene of a crime. Researchers at the University of California, Berkeley, developed the device, which packs microfluidics, electronics, optics, and chemical detection technology into a single briefcase-sized unit. "While previous groups have developed lab-on-a-chip systems, none of them have succeeded in making a completely portable, robust system that can be used at a scene," says team leader Richard Mathies. On-site CSI: After a blood sample is collected from the scene of a crime, this briefcase-sized [more...]

Date: 2008-09-24 04:00:00

PLoS ONE: Evidence of Authentic DNA from Danish Viking Age ...

Linea Melchior1, Toomas Kivisild2, Niels Lynnerup3, Jørgen Dissing1*1 Research Laboratory, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark, 2 Leverhulme Center for Human Evolutionary Studies, The Henry Wellcome Building, University of Cambridge, Cambridge, United Kingdom, 3 Laboratory of Biological Anthropology, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark Abstract Top Background Given the relative abundance of modern human DNA and the inherent impossibility for incontestable proof of authenticity, results obtained on ancient [more...]

Date: 2008-05-28 07:00:00

Preparation of DNA Ladder Based on Multiplex PCR Technique

PCR is a simple, effectively and conveniently method and could prepare amount oligonucleotides in a very short time. However, preparing DNA ladder using common PCR was laborious. Different standard fragments were mixed according to some [more...]

Date: 2010-05-27 07:00:00

Research Technician vacancy in Biology at Univ Hospital Zurich ...

Applicants should hold either a degree as professional technician (e.g. SRK), or an MSc in Biology, and must have a strong practical background in the following technologies: genomic DNA extraction/purification, quantitative PCR and good computer knowledge. Experience with robotic equipment and automation are an advantage. Skills in laboratory management, high level of self-organization and interpersonal skills to work within an international group of scientists are a plus. Fluent technical English and German are obligatory. Applicants should submit a letter of interest, CV, working [more...]

Date: 2010-10-11 22:47:53

Arrestin translocation is stoichiometric to rhodopsin ...

By RT-PCR and DNA sequencing we ... the first molecular evidence that components of a non-visual phototransduction pathway are expressed in the human ocular NPE ciliary epithelium ... Protein content and cAMP-dependent phosphorylation of fractionated white perch r Brain Res Feb 13, 1995 ... of 30/31 kDa, 35 kDa (putative rhodopsin) and 48 kDa (putative arrestin) which coenrich with photoreceptor fractions. The 43/44 kDa phosphoprotein is a target for cAMP-dependent protein phosphorylation and thus is apparently ... Protein kinase C activity and light sensitivity of single amphibian rods. J [more...]

Date: 2010-10-03 16:09:20

Lab Research Technician (LRA I) (Duke University Medical Center ...

-Must be skilled in basic molecular biology techniques including DNA purification, PCR, and cloning Ideal skills: -Experience working with rodents, including colony maintenance and embryology -Experience with mammalian cell culture and microscopy -Two-year commitment preferred If you are interested please Apply here to Lab Research Analyst Req#400439061 (https://sjobs.brassring.com/1033/ASP/TG/cim_jobdetail.asp?partnerid=25017&siteid=5172&AReq=33156BR). Please include your CV and contact information for three references. For more information, please refer to our website at: [more...]

Date: 2010-10-11 18:43:41

Pharmalot… Pharmalittle… Good Morning // Pharmalot

There are two main technologies involved: RT-PCR (reverse transcription polymerase chain reaction) and DNA microarray. What research scientists in universities and cancer centers have been doing for the past ten years is to try and figure out a way to use this technology to look for patterns of gene expression which correlate with and predict for the activity of anticancer drugs. However, genes do not operate alone within the cell but in an intricate network of interactions. Since the new millenium there has been the increasing acceptance of the concept that cancer is a very heterogenous [more...]

Date: 2010-10-11 11:40:52


FIRST HIV PATIENT NOT DETECTED BY HIV DNA PCR ANTIGEN TEST HIV Patients who were earlier confirmed positive for HIV either through DNA PCR test or Western blot Antibody test after undergoing treatment with HOO-IMM PLUS from HOOTONE [more...]

Date: 2010-09-04 07:36:45

Molecular Clinical Lab Scientist

This achievement was unthinkable only 5 years ago. Be part of a leading edge team working with the best genomic tools. Duties include: Perform all aspects of genetic testing such as DNA extraction, PCR, [more...]

Date: 2010-09-19 23:18:22

Basic Principles

Nuclear or genomic DNA is found in all nucleated cells as well as in the reproductive cells (eggs and sperm). The amount of DNA we can expect to find in different cells and types of evidence is found in Table 1. ... The second method is a specialized 'nonorganic' extraction using Chelex beads. Chelex beads can only be used when PCR-based DNA testing is going to be used. The basic DNA extraction procedures, whether organic or non-organic, can be adapted for special [more...]

Date: 2010-08-01 06:59:59

Aptamer Project Site - 10% APS for Everyone: Progress: EphA2 ...

The more rounds of PCR needed to amplify the DNA indicates a decrease in the amount of background binders and decreasing variability in the pool, highlighting the potential impact of aptamers on the medical world. Because the binding assay indicated that there was a low amount of binders after 5 rounds of selection, a new selection was begun. To follow the experimental conditions of Sharon Reimann, a 2008 student who achieved ~10% binding after 5 rounds of selection using the N35 pool and EphA2 through filter based selection, filter based selection on the N34 pool and EphA2 was begun today. [more...]

Date: 2010-10-07 20:44:00

Cytoplasmic & Nuclear RNA Purification Kit

RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]

Date: 2010-08-16 04:58:00

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