Our blogs and videos choice: pcr dna test

ERV's latest blog on the FDA paper. One step too far?.

PNAS paper, using standard PCR to ID 'positives' and 'negatives', with insane numbers of bands as a result of non-specific primer binding. Insane numbers. As if this is 1990 and we dont know what 'Real Time PCR' and 'Taqman Probes' are. Im going to ignore that and grant the premise they really have IDed viral sequences. And they arent XMRV. They dont cluster anywhere near XMRV on a phylogenetic tree. Theyre something else entirely. Okay, well, logically it could be contamination from mouse DNA. So they checked for that... by looking for mouse mitochondrial DNA. *blink* I understand [more...]

Date: 2010-08-23 07:00:00

Blog posts (43) | Videos (10)
 


An ELISA-based high throughput protein truncation test for ...

Here, we report the next generation HTS-PTT using triple-epitope-tagged proteins and demonstrate, for the first time, its efficacy on clinical genomic DNA samples for BRCA1/2 analysis. Methods: Segments of exons 11 of BRCA1/2 open reading frames were PCR amplified from either blood derived genomic DNA or cell line mRNA. PCR primers incorporate elements for cell-free transcription/translation and epitope tagging. Cell-free expressed nascent proteins are then antibody-captured onto the wells of a microtiter plate and the relative amount of truncated polypeptide measured using antibodies against [more...]

Date: 2010-10-04 16:47:13


Case Analyst (Salt Lake City)

Successful candidate will perform genotyping analysis. Experience using GeneMapper genotyping software a plus. Experience performing DNA extraction, PCR and genotyping for STR analysis and mitochondrial sequencing essential. Other lab duties as directed. Required: • BS in Biochemistry, Molecular Biology, Biology or related field • 1 year experience in laboratory • Knowledge of molecular biology testing, analysis and reporting. • Must be able to use hands, stand for periods of time and lift up to 20 lbs. Preferred: • Computer and Microsoft applications proficiency • 2 years [more...]

Date: 2010-10-01 04:43:12


Basic Principles

Nuclear or genomic DNA is found in all nucleated cells as well as in the reproductive cells (eggs and sperm). The amount of DNA we can expect to find in different cells and types of evidence is found in Table 1. ... The second method is a specialized 'nonorganic' extraction using Chelex beads. Chelex beads can only be used when PCR-based DNA testing is going to be used. The basic DNA extraction procedures, whether organic or non-organic, can be adapted for special [more...]

Date: 2010-08-01 06:59:59


FIRST HIV PATIENT NOT DETECTED BY HIV DNA PCR ANTIGEN TEST

FIRST HIV PATIENT NOT DETECTED BY HIV DNA PCR ANTIGEN TEST HIV Patients who were earlier confirmed positive for HIV either through DNA PCR test or Western blot Antibody test after undergoing treatment with HOO-IMM PLUS from HOOTONE [more...]

Date: 2010-09-04 07:36:45


ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...

Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]

Date: 2010-08-25 00:51:13


Molecular Clinical Lab Scientist

This achievement was unthinkable only 5 years ago. Be part of a leading edge team working with the best genomic tools. Duties include: Perform all aspects of genetic testing such as DNA extraction, PCR, [more...]

Date: 2010-09-19 23:18:22


New Australian UFO Site (UFOESA) - Alien UFOs

T.c T.c is offline. Astronomical Unit. ★ Star Contributor. T.c has much to be proud of. T.c's Avatar. Posts: 2155. Join Date: May 2004. Country: Australia. Default New Australian UFO Site (UFOESA) - 10-07-2010, 10:15 PM ... This is one of them, it has the worlds first PCR DNA test from an alleged Visitation. I just finished uploading the UFO site for Peter Khoury. When I have time I will be adding the scientific equipment used in the PCR/DNA testing by Australian [more...]

Date: 2010-10-08 03:15:40


PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading

Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]

Date: 2009-11-13 08:00:00


A day in the life of… Sara

I prepare the appropriate master mixes and primer probes for my samples to prepare them for DNA amplification.  I can test for DNA amplification, and therefore if my primers worked, by running an agarose gel electrophoresis on the PCR product against DNA ladder standard material.  After sending an electrical current through my gel and buffer, I observe how chunks of DNA from the PCR product have moved (or raced) through the gel relative to the standards (I hope to see a tie at a certain ‘ladder step’ in each lane). At this point in my day I secretly do a celebratory fist pump and heel [more...]

Date: 2010-07-22 23:14:43


バイオとメディカルの特許翻訳の長文例(対訳中心:バイオ、医学、医薬 ...

対照的に、低リスクのHPV、例えばHPV-6及びHPV-11は、滅多に癌を発症させない。低リスクのHPV及び高リスクのHPVの存在が、ポリメラーゼ連鎖反応(「PCR」)又はHybrid Capture(登録商標) II HPV Test (「HC II HPV Test」;Digene Corp., ..... シグナル増幅法 の当該技術分野において既知の例は、分枝DNA(bDNA)シグナル増幅(Bayer Healthcare LLC, Tarrytown, N.Y.)、Hybrid Capture(「HC」;Digene Corp., Gaithersburg, MD.)、及びTyramideシグナル増幅(「TSA」Perkin Elmer, Inc., Wellesley, MA)。 [more...]

Date: 2010-09-29 12:17:28


ISPUB - Comparison and Development of A Rapid Extraction Methods ...

Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological, Archaeological, Museum, Medical and Forensic Specimens. New York, NY: Springer Verlag. (s)7. Holland, M.M. and T.J. Parsons. 1999. Mitochondrial DNA sequence analysis— validation and use for forensic casework. Forensic Science Review. 11: 22 – 49. (s)8. Matysiak-Scholze,U., Dimmeler,S. and Nehls,M. (1996) Technical Tips Online, T40011. (s)9. Near T.J., Bolnic D.I. and Wainwright P.C., Fossil calibrations and molecular divergence time estimates in centrarchid fishes, Evolution, 2005, 59(8): 1768-1782 (s)10. Paabo [more...]

Date: 2010-06-06 01:06:13


Ensuring Successful PCR Using Online Resources-eNotes

This system quantitates DNA prior to multiplex short tandem repeat (STR) amplification, a method used for DNA typing, including paternity testing, forensic DNA analysis and human identity testing. [more...]

Date: 2009-04-14 00:00:01


TriMark Publications Announces Release of Its Nutraceuticals and ...

Packed with the latest information relating to new products and industry trends, this analysis not only quantifies but also qualifies the nutraceuticals market in the areas of research, product development and investment opportunities. ... DNA Sequencing and PCR Markets - Genomics World Markets - High-Growth Diagnostic Tests Markets - Indian Pharmaceutical Industry - Key Diagnostic Testing Markets - Mammography World Markets - Medical Imaging Markets - Microarray Markets [more...]

Date: 2010-10-11 21:42:02


Plasmid double locus sequence typing for IncHI2 plasmids, a ...

Plasmid double locus sequence typing for IncHI2 plasmids, a subtyping scheme for the characterization of IncHI2 plasmids carrying extended-spectrum β-lactamase and quinolone resistance genes Aurora García-Fernández and Alessandra Carattoli* Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy *Corresponding author. Tel: +39-06-49903128; Fax: +39-06-49387112; E-mail: alecara{at}iss.it Received October 9, 2009. Revision requested January 25, 2010. Revision received February 26, 2010. Accepted March 4, 2010. Abstract Objectives IncHI2 [more...]

Date: 2010-03-31 07:00:00


New DNA Diagnostic Test For Lyme Disease May Have Merit

Milford Hospital in Massachusetts has been working for over a year on a new type of diagnostic test for Lyme disease using DNA sequencing rather than DNA nesting. Huh? We have all heard of the polymerase chain reaction (PCR) method of [more...]

Date: 2010-09-17 03:38:41


HTB – Early infant diagnosis

She concluded that promising POC assays for EID today include: IQuum’s LIAT, SAMBA, CIGHT p24 and possibly CLONDIG’s viral load assay. comment This was an incredibly useful overview and it looks like we can be optimistic about having a POC test for EID in the next couple of years. Other presentations at the conference dealt with the challenges of access to EID. In the same session, Shaffiq Essajee noted that EID access has improved and in some countries more than 50% of exposed infants are tested. But infant testing is usually linked to PMTCT and if coverage is low, so is infant [more...]

Date: 2010-09-30 06:04:59


Extraction of DNA from Fish Fins (Amrita University)

Many different methods and technologies are available for the isolation of genomic DNA. Here, we describe a method for the isolation of PCR-ready genomic DNA from zebra-fish tissues. Video Rating: 0 / 5Source: DNA [more...]

Date: 2010-10-09 19:26:00


TropIKA: Molecular test for TB performs strongly in evaluation study

It uses polymerase-chain-reaction (PCR) technology to detect the DNA of the TB bacterium. In addition to detecting the presence of M. tb., the test is intended to determine whether the strain of the organism found is resistant to rifampicin , a mainstay of current TB ... The addition of a second MTB/RIF test increased sensitivity in smear-negative patients by 12.6 percentage points, and a third test increased it by a further 5.1 percentage points to a total of 90.2%. [more...]

Date: 2010-09-27 03:00:00


DNA saliva samples for SNP and CNV analysis on microarrays

Studies show that DNA from Oragene saliva samples gives equivalent results to DNA from blood for PCR, SNPgenotyping and microarrays. ... For any type of genetic analysis (e.g. population studies, clinical genetic testing, paternity testing, pharmacogenomic testing), non-invasive and easy-to-use sample collection techniques are preferred because they increase compliance rates and reduce costs. Oragene•DNA is a DNA self-collection kit that is completely non-invasive, [more...]

Date: 2010-10-05 15:34:00


Paternity Blood Test | Diet and Health Tips & Products Reviews

Today, the most accurate tests (99.99%) to determine the paternity of a child are made through DNA. Collecting DNA sample is done two ways: Buccal swab (swabbing the inside of the cheek) and blood samples. Known as PCR (polymerase chain reaction), the procedure is used in comparing samples of the childís DNA with that of the alleged father. Since the childís DNA is a fairly equal combination of DNA of his parents, excluding the DNA of the mother gives us a genetic profile of the biological father. However, before science knew how to isolate DNA, blood samples were the standard in a variety [more...]

Date: 2010-04-29 03:30:53


DNA Preparation of Bone Marrow Sample for Backtracking | Cancer ...

To isolate DNA from supernatant, the 500µl of 2X concentration of PBS is used.4. 500µl of PCI was added into the tube for 30 minutes.5. Later, the tube was spun at 10000X ϑ for 10 minutes.6. The aqueous supernatant was removed to a new tube without disturbing the PCI layer.7. The PCI layer was discarded and add in 1:10 volume of 10M NH4OAc into the tube to mix.8. Next, the 2X volume of ethanol was added and mixed up the ethanol and precipitated DNA.9. The ethanol was removed from the mixture. The precipitation was washed one time with 500µl 70% ethanol.10. Again, the ethanol was removed [more...]

Date: 2010-07-16 08:22:19


DNA Testing FAQ « Colorado Health Screenings

DNA testing is the most conclusive and widely accepted method for testing biological relationships. Our lab uses PCR (Polymerase Chain Reaction) method which is the most advanced technology; therefore, we can provide the most accurate, definitive results in the industry. We guarantee results for legal testing with a probability of 99.99% or greater for inclusion and 100% certainty for exclusion. When May Testing be Performed? There are no age restrictions; because DNA makeup is determined at conception. Testing is possible even before birth, and newborns may be tested safely at delivery using [more...]

Date: 2010-09-23 12:59:45


FDA Approves Roche's Hepatitis B Viral Load Test | InfoGrok

The new Roche test provides a fully automated solution for the quantitative detection of hepatitis B virus (HBV) DNA in human plasma or serum for patients on HBV antiviral therapy. The Roche COBAS AmpliPrep / COBAS TaqMan HBV Test v2.0 has been validated to quantify diverse samples from genotypes A-H and pre-core mutants across a broad linear dynamic range of 20 IU/mL to 1.7E+08 IU/mL. The new assay uses a reduced sample input volume of 650 uL of either serum or plasma specimens and is standardized against the World Health Organization (WHO) standard for hepatitis B. This test is designed for [more...]

Date: 2010-09-23 14:10:06


MTHFR A1298C polymorphism and idiopathic male infertility Singh K ...

The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyses the formation of folate intermediates that are vital for DNA synthesis and methylation reactions. C677T and A1298C variants of MTHFR result in reduced plasma folate and increase the susceptibility to various multifactorial disorders. We have already shown that homozygosity for 677 (C ®T) mutation in the MTHFR gene, is a risk factor for idiopathic male infertility in an Indian population. Aim: Recently, we showed that homozygosity for the 677(C;T) mutation in the MTHFR gene is a risk factor for idiopathic male infertility and [more...]

Date: 2010-10-08 20:51:33


How Fingerprinting Helps In DNA Paternity Testing

This form of fingerprinting method is also known as DNA profiling. Using this technique, the individuals are identified on the basis of their unique DNA profiles. This DNA profiling is done by analyzing the VNTR sequences. It stands for variable number tandem repeats. This sequence is found to be different in individuals except in twins. As per the facts mentioned above, the DNA samples of the child will exhibit the similar VNTR loci as that of their parents. This fingerprinting helps in identifying that whether the known father of a child is genuinely his biological dad. In fact, it is [more...]

Date: 2010-08-09 11:41:10


Research Associate (San Diego) | PCR Jobs

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:  Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.  Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.  Prepares technical reports, summaries, protocols and quantitative analyses.  Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]

Date: 2010-10-01 19:07:24


Thrombosis Journal | Full text | Effect of PlA1/A2 glycoprotein ...

Genomic DNA was prepared from peripheral lymphocytes by the salt precipitation method . The PlA1/A2 alleles of the GP IIIa gene were identified on the basis of MspI enzyme site restriction analysis after amplification of a 476 base pairs GP IIIa fragment (sens amorce 5'-ATA-AGC-TTA-GCT-ATT-GGG-AAG-TGG-TAG-GGC-CTG-3', antisens amorce 5'-CTT-CTG-ACT-CAA-GTC-CTA-ACG-3'). The GP IIIa gene was amplified using the polymerase chain reaction (PCR) method. Each amplification product was verified on an agarose gel. Amplification results in a 476 base pairs (bp) fragment. Digestion was obtained with [more...]

Date: 2008-01-15 08:00:00


Is free, circulating DNA a useful marker for prostate cancer ...

The "New" Prostate Cancer InfoLink has been developed to become a primary source of accurate, current, and topical information about prostate cancer for patients and their families. The "New" Prostate Cancer InfoLink is intended for informational purposes only. It is not engaged in rendering medical advice or professional services. News and information provided on this site should not be used for diagnosing or treating any health problem or disease. The "New" Prostate Cancer InfoLink is not a substitute for professional care. If you have or suspect you may have a health problem, please [more...]

Date: 2010-09-28 12:30:40


NedayeFan Co

T4 Polynucleotide Kinase 500 u EK0031 450,000 T4 DNA Ligase (With PEG) 200 u EL0335 450,000 T4 DNA Ligase 200 u EL0015 450,000 T4 DNA Polymerase 100 u EP0061 440,000 Taq DNA Polymerase (native) 100 u EP0281 470,000 Taq DNA Polymerase (native) 500 u EP0282 1,880,000 Taq DNA Polymerase (recombinant) 100 u TA7505C 50,000 Taq DNA Polymerase (recombinant) 5,000 TA7507C 1.200,000 Taq DNA Polymerase (recombinant) 500 u TA7506C 160,000 TaqI 3,000 u RE7717C 230,000 TBE Buffer, 5X 50 ml MR7725C 20,000 Total Protein (Automatic and manual) 300Tests BK8145C 25,000 Triglycerides (Automatic and manual) New [more...]

Date: 2005-10-25 10:14:00


GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...

GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are  involved in various disease problems in humans and aquatic  animals and are known to be  phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific  diagnostic test is warranted for detection and characterization of this pathogen.In the present study,  Fish  and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]

Date: 2010-10-08 20:08:05


Thrombosis Journal | Full text | Expression of sterol regulatory ...

DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]

Date: 2009-02-18 08:00:00


Rapid Detection of Respiratory Tract Viral infections and Co ...

Respiratory samples were tested using the combination of two commercially available RT-PCR DNA microarray systems allowing a rapid detection of influenza A strains including the new A/H1N1v strain as well as twenty other common  or newly discovered respiratory viruses. Concomitantly a generic and classical real time RT-PCR assay was performed to detect all the circulating influenza A strains in the same samples. Of the 95 respiratory samples tested, 30 (31%) were positive for  the detection of influenza A/H1N1v infection by both RT-PCR DNA microarray and classical real time RT-PCR detection [more...]

Date: 2010-08-28 06:34:00


Intellectual Property Expert Group (ipeg) » Blog Archive » Are the ...

These are just two recent cases in the US but the implication on DNA sequence patents may be far reaching. Back in 2000, when the new economy was still alive, there was a lot of focus on DNA sequence patents. A lot of companies were very cautious and a lot of genetic test developments were stalled because of patent issues. When asking an expert you could get philosophical answers: either you licence or you ignore or you litigate. Now in 2000 there were still patents from the early days of DNA sequencing around. Some of these patents might have been inventive as the inventive step was a lot [more...]

Date: 2010-09-20 16:55:26


X-Tagged.com Adult Social Bookmarking - DNA sequencing

PCR Analysis facilitates the capacity to make millions of DNA copies from a single biological sample. This type of analysis enables the forensic scientist to examine small data such as a few skin cells. It also lessens the chances of contamination of the sample, but it requires very meticulous work. Short tandem repeat (STR) technology Short tandem repeat (STR) technology analyzes all 13 Loci (regions )for DNA testing and is the technology used by the FBI databases at the local, state, and federal level. Short tandem repeat (STR) technology for DNA profiles from convicted offenders, unsolved [more...]

Date: 2010-10-12 18:10:35


Blood test detects HIV/AIDS in infants aged 6 months in India DNA ...

Unlike other tests, the HIV DNA PCR test does not rely on the presence of antigens or antibodies in the blood for diagnosis. Instead, it identifies genetic material by highlighting sequences of the virus within the subject’s DNA. All babies born to mothers with HIV are born with HIV antibodies. The currently available tests can predict the chances of HIV infection in infants borne to infected mothers only after the completion of 18 months. This delay in diagnosis often caused a problem for doctors because they found starting the treatment using the life-saving Anti-Retroviral Treatment [more...]

Date: 2010-07-19 22:55:54


Methylation Test May Aid Colon Cancer Diagnosis (CME/CE) | Discuss ...

To develop a more sensitive test for DNA methylation, investigators employed a form of technology known as BEAMing (beads, emulsion, amplification, and magnetics). They performed PCR amplification of individual DNA fragments covalently linked to magnetic beads suspended in water-based nanoparticles immersed in oil droplets. The beads contained a DNA sequence specific for exon 1 of the vimentin gene, which is hypermethylated in colon cancer. Following PCR amplification, the beads were hybridized by means of fluorescent probes specific to the state of methylation and then sorted and analyzed by [more...]

Date: 2010-10-09 11:00:27


Variants of PCR (1)

The bacteriophage T4 DNA polymerase was also tested shortly after the first reports of PCR. It has a higher fidelity of replication than the Klenow fragment. Since it is also destroyed by heat, it has seen little use since the development of thermostable polymerases. The DNA polymerase from Thermus aquaticus (or Taq), was the first thermostable polymerase used in PCR, and is still the one most commonly used. The enzyme can be isolated from its 'native' bacterial source, or from a cloned gene expressed in E. coli. The Stoffel fragment is produced from a truncated gene for Taq polymerase, [more...]

Date: 2008-09-11 03:56:00


Evaluation of Five Methods for Total DNA Extraction from Western ...

DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. Methodology/Principal Findings From individual western corn rootworm beetles, Diabrotica virgifera virgifera, DNA extractions by the SDS method, CTAB method, DNAzol® reagent, Puregene® solutions and DNeasy® column were compared in terms of DNA quantity and quality, cost of materials, [more...]

Date: 2010-08-13 07:00:00


Forensic Serology Information Page

What is Forensic Serology?According to forensic serologist Marcella Jones of www.ForensicMentor.com, forensic serology is the analysis of body fluids as they relate to forensic cases, including DNA analysis. Accordingly, the role of the forensic serologist involves:Examining evidence for the presence of body fluids e.g. blood, semen, hair, tissue, saliva, feces, and urineEvaluating evidence for potential DNA analysisEvaluating species of body fluidsPerform PCR (polymerase chain reaction) based typing of STR (short tandem repeat sequences) of genomic DNA, or mitochondrial sequence analysis of [more...]

Date: 2010-02-04 08:00:00


- “Paternity Testing”

Paternity testing results? - Yahoo! UK & Ireland Answers Does anybody know how reliable dna testing was in 1982?, could it have … Seriously i think you would be better to ask a Medical Doctor, rather then random ppl … uk.answers.yahoo.com/question/index?qid… Advanced Testing Center - 10 Commercial St , Foxboro, MA 02035 … … igm test pcr test hiv rna test igg testing igm testing pcr testing dna test dna testing confidential herpes testing paternity test paternity testing. … www.getfave.com/12341786-advanced-testing-center court- ordered paternity testing in michigan? how would [more...]

Date: 2010-04-29 23:36:17


Information About Non Paternity And Non Maternity

All the same, requirements for consent and counselling for DNA testing are unique from each country's laws and policies. DNA testing would be the most state-of-the-art and precise technique to figure out both non-paternity and non-maternity. In figuring out if the presumed parents are genuine biological ones or in determining actual biological mother or father-child bond, subjects undergo parental screening. This sort of practice uses the genetic fingerprinting (or DNA profiling), that is a approach to recognize identity by means of DNA profiles. A paternity test serves as an inherited proof [more...]

Date: 2010-10-09 18:01:01


Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...

Genetic Fingerprinting of Bacillus thuringiensis Isolates by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)(Published in Nepal Journal of Science and Technology 10(2009) 97-103)Gyan Sundar Sahukhall, Upendra Thapa Shrestha1, Binod Lekhak2, Anjana Singh2, Viswanath Prasad Agrawal11Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal. 2Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepale-mail: gyan633413@gmail.com AbstractRandom Amplified Polymorphic DNA (RAPD) is a method of producing a genetic fingerprint of a [more...]

Date: 2010-01-21 14:06:00



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