Our blogs and videos choice: mitochondrial dna analysis

Ancestry DNA Testing| Information | homeDNAdirect.com.au Blog

Ancestry DNA testing: A General Overview Posted on | August 13, 2010 | No Comments There are many reasons to carry out ancestry DNA testing; ancestry testing can tell you so much about your history and ancestors, that which you cannot find out any other way than by doing such a test. The test will link you to your deep and far back geogenetic roots going back thousands and thousands of years. Moreover, it can help all the more those who are clueless or simply wish to know more about their ancestral origins. To trace you ancestral origins, you will be provided with an ancestry testing kit. [more...]

Date: 2010-08-13 10:50:48

Blog posts (27) | Videos (14)

Ancestry DNA Testing

DNA research worldwide led to the discovery of genetic markers that are used to evaluate ancestry. These markers follow three types of inheritance pattern categories. These are autosomal DNA line, the paternal DNA line and maternal DNA line. The autosomal DNA line is made of all DNA in the cells excluding the X – chromosome, Y – chromosome and mtDNA. A person inherits half of autosomal DNA from the biological mother and the other half from the biological father. The Y chromosome markers are paternally related and passed from a father to his son. They are used to trace a person’s [more...]

Date: 2010-09-25 18:36:37

Can DNA Tests Reveal Nationality? » Scienceline

The Borders Agency is primarily using two types of DNA tests: mitochondrial DNA tests, which examine the genetic information stored in a cellular structure called a mitochondrion, and what are called Y-line tests, which look at markers on the Y-chromosome passed from father to son. These tests have been widely used to examine people’s ancestral heritage. Since certain genetic variations are typical in certain areas, geneticists can use an individual’s DNA to estimate where their ancestors came from. But when this technique is used to look at someone’s nationality rather than the origin [more...]

Date: 2009-12-30 06:05:09

Press Release – Introducing Lean Six Sigma Consulting Program

Sorenson Forensics provides services, such as STR and Y-STR analysis; mitochondrial DNA sequencing; comprehensive biology screening; full serology testing, including blood, semen and other body fluid identification; DNA case [more...]

Date: 2010-09-23 20:29:15

DNA method gives new perspective on the Mysteries of Nature

We demonstrate that this new approach enables the assignment of virtually all the generated DNA sequences to the correct source once sequencing anomalies are accounted for (miss-assignment rate less than 0.4%). Therefore, the method enables accurate sequencing and assignment of homologous DNA sequences from multiple sources in single high-throughput GS20 run. We observe a bias in the distribution of the differently tagged primers that is dependent on the 5' nucleotide of the tag. In particular, primers 5' labelled with a cytosine are heavily overrepresented among the final sequences, while [more...]

Date: 2007-02-15 17:01:00

Origin of the human malaria parasite in gorillas - TalkRational

But researchers from the US, three African countries, and Europe have examined malaria parasites in great ape faeces. They found the DNA from western gorilla parasites was the most similar to human parasites. Liu, W. et al. (2010) Origin of the human malaria parasite Plasmodium falciparum in gorillas. Nature, 467, 420-425. Plasmodium falciparum is the most prevalent and lethal of the malaria parasites infecting humans, yet the origin and evolutionary history of this important pathogen remain controversial. Here we develop a single-genome amplification strategy to identify and characterize [more...]

Date: 2010-09-22 17:35:43

ISPUB - What's There In Our Genes? A Case For A More Inclusive And ...

We need better reviews, use of both qualitative and quantitative data as well as much more rigorous testing of newer and older models. Only then may we make full use of this large amount of data that has already been collected over the years. What we have described here, for now, presents part of the picture seen from the Indian subcontinent as an example. However, this situation is probably true of other parts of the world as well, perhaps even those areas where it has been assumed that the data is likely to be homogenous. Thus, even biological anthropologists need to add new sensitivities [more...]

Date: 2010-10-07 04:39:48

Genome Biology | Full text | Population phylogenomic analysis of ...

The time of divergence between European and Asian pig mitochondrial DNAs (mtDNAs) was long before the time of possible pig domestication, which supported the independent origin of domestic pigs in Europe and Asia . ... The NJ tree of 50 mtDNA genomes (including 24 published near complete sequences; Additional data file 2) revealed two major clades, E and A, which represent the wild boars (Sus scrofa) and domestic pigs from Europe and East Asia, respectively (Figure [more...]

Date: 2007-12-18 08:00:00

Dienekes' Anthropology Blog: Fine resolution study of mtDNA ...

Haplogroup H (hg H) includes about 40-50% of the West Eurasian mitochondrial DNA (mtDNA) samples investigated so far. In order to enhance discrimination within this haplogroup we selected 45 coding region SNPs that allow to ascribe samples to the main phylogenetic branches of super hg HV (that embraces hg H) and, in particular, to H sublineages with a much finer resolution than previous studies. SNP selection was carried out using the most up-to-date available literature on population and forensic genetics and extended by means of phylogenetic analysis of complete or coding region genomes [more...]

Date: 2006-05-26 16:57:00

Dienekes' Anthropology Blog: Complete Neanderthal mtDNA sequence

A complete mitochondrial (mt) genome sequence was reconstructed from a 38,000 year-old Neandertal individual with 8341 mtDNA sequences identified among 4.8 Gb of DNA generated from ∼0.3 g of bone. Analysis of the assembled sequence unequivocally establishes that the Neandertal mtDNA falls outside the variation of extant human mtDNAs, and allows an estimate of the divergence date between the two mtDNA lineages of 660,000 ± 140,000 years. Of the 13 proteins encoded in the mtDNA, subunit 2 of cytochrome c oxidase of the mitochondrial electron transport chain has experienced the largest number [more...]

Date: 2008-08-07 16:44:00

Age-Related Macular Degeneration — NEJM

When it reaches a critical concentration, A2E inhibits the proton pump of lysosomes,25 causing leakage of the contents of the lysosomes into the cytoplasm of RPE cells.23 A2E can also damage the DNA in RPE26 and mitochondrial membranes. .... There is reproducible evidence of a consistent association between smoking and age-related macular degeneration.100101 Analysis of pooled data from three population-based cohort studies showed that the relative risk of late [more...]

Date: 2006-10-05 05:00:00

DefrostingColdCases.com » Blog Archive » FBI's forensic DNA case load…

The FBI’s forensic DNA case load is so backlogged that it would take two years to complete – even without any new cases added, according to a new report released by the Department of Justices’ inspector general.More than 3,200 backlogged DNA cases are still awaiting analysis as of March, and the number is growing. DNA analysis is a lengthy process, and it can take up to 600 days for FBI labs to provide testing results.According to the report, missing persons cases represent the biggest portion of backlogged cases, The average time that it takes the FBI Laboratory to provide DNA testing [more...]

Date: 2010-08-10 01:22:10

How Fingerprinting Helps In DNA Paternity Testing

This form of fingerprinting method is also known as DNA profiling. Using this technique, the individuals are identified on the basis of their unique DNA profiles. This DNA profiling is done by analyzing the VNTR sequences. It stands for variable number tandem repeats. This sequence is found to be different in individuals except in twins. As per the facts mentioned above, the DNA samples of the child will exhibit the similar VNTR loci as that of their parents. This fingerprinting helps in identifying that whether the known father of a child is genuinely his biological dad. In fact, it is [more...]

Date: 2010-08-09 11:41:10

Book Review-Portrait of a Killer-Jack the Ripper-Case Closed by ...

She explains that it is like an egg where nuclear DNA would be found in the yolk, whereas mitochondrial DNA would be found in the egg white. Apparently, mitochondrial DNA is passed down only from the mother. This is the sort of analysis that Cornwall delves into. I won’t be giving anything away by telling you that Cornwall names world famous artist of the time (the late 1800’s) Walter Sickert as Jack the Ripper. She tells you herself on the inside dust jacket of the book. What is intriguing is the way that Cornwall arrives at this conclusion. She compares handwriting, tests DNA on [more...]

Date: 2010-10-08 07:33:41

dna sequencing

DNA sequencing reactions all use a primer to initiate DNA synthesis.  A Quicktime animation of how dideoxy nucleotides are incorporated into DNA in sequencing reactions. Nucleic acid sequence This sequence is written as a succession of letters representing a real or hypothetical DNA molecule or Sequences can be read from the biological raw material through DNA . DNA Sequencing – Real Time DNA analysis Information With the latest chellenges in DNA Sequencing, our organization looks to unravel the myths, truths and most essential assets in the deconstruction of genes. DNA Sequencing The [more...]

Date: 2010-07-09 03:20:29

Dienekes' Anthropology Blog: mtDNA of Yemeni and Ethiopian Jews

The way I see it, only a large-scale study of all global Jewish populations may uncover verified ancient Jewish lineages for both Y-chromosomes and mtDNA. The recent studies on Jews have uncovered several genetic sub-clusters of Jews, and only lineages that occur in 2 or more of these clusters, and preferably geographically separated ones have a strong claim of representing original Jewish lineages. There is a limit on what can be uncovered about the past from the study of living populations. American Journal of Physical Anthropology doi: 10.1002/ajpa.21360 Mitochondrial DNA reveals distinct [more...]

Date: 2010-07-14 18:00:00

Gene Sequencing: DNA analysis

Not everyone can properly and correctly analyze the DNA. Apartyears of study devoted to knowledge of DNA, DNA analysts to go through years of experience and practice in laboratories and practice of DNA testing and analysis in order to analyze the DNA and how it works. The training of DNA analysts understanding of sophisticated tools and machines developed for DNA analysis. proper management and use of these devices would make them more efficient in the pursuit of DNA to be analyzed correctly. Most of the DNA consists of 23Pairs of chromosomes, mitochondrial DNA inherited from the mother, and [more...]

Date: 2010-09-22 05:00:00

Interview with Arun Kumar: Genographic Postdoc, India - Genographica

I have worked with state of the art DNA technologies like Taqman assays, Fragment Analysis and DNA sequencing and have gained a true global experience working with on the Genographic team. Everyday brings a new learning experience! GP: Wait a minute, for those of us that aren't scientists, what is an assay? Arun Kumar: An assay is a way to genotype a specific mutation within DNA. In other words, it is an experiment that helps to identify a specific nucleotide with Y chromosome or mitochondrial DNA. This mutation will define the ancestral or derived state which will help to identify the [more...]

Date: 2010-10-07 19:07:51

The Journey of Man: A Genetic Odyssey

A very nice book if one is interested in understanding how DNA analysis is being used to trace human migration and from where it started. Would like more substance. There is no in-depth explanation of mitochondrial DNA dating, Y-chromosome dating, etc. More red meat than Seven Daughters of Eve for sure! Does this book have. Interesting. I thought it was a good book- and being a molecular biologist who is familiar with the worlds advanced knowledge of genetics, I am quite comfortable with my African origins and the obvious fact that we (meaning all races) are all extremely similar. Fascinating [more...]

Date: 2010-05-23 10:41:42

Colony PCR: Another Use For Toothpicks « Promega Connections

Colony PCR: Another Use For Toothpicks July 26, 2010 by Isobel I still remember my JOY (sadly, yes) when I first learned about colony PCR. It allowed me to avoid a laborious procedure involving genomic DNA isolation, restriction digestion, and (the dreaded) Southern blotting. I was trying to show definitively that a transposon had successfully been inserted into a target gene. Using colony PCR, I could just amplify the DNA from a colony and then show that the transposon increased the size of the expected PCR product compared to a control. Joy. I could also use colony PCR to screen DNA [more...]

Date: 2010-07-26 15:13:31

ISPUB - Comparison and Development of A Rapid Extraction Methods ...

Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological, Archaeological, Museum, Medical and Forensic Specimens. New York, NY: Springer Verlag. (s)7. Holland, M.M. and T.J. Parsons. 1999. Mitochondrial DNA sequence analysis— validation and use for forensic casework. Forensic Science Review. 11: 22 – 49. (s)8. Matysiak-Scholze,U., Dimmeler,S. and Nehls,M. (1996) Technical Tips Online, T40011. (s)9. Near T.J., Bolnic D.I. and Wainwright P.C., Fossil calibrations and molecular divergence time estimates in centrarchid fishes, Evolution, 2005, 59(8): 1768-1782 (s)10. Paabo [more...]

Date: 2010-06-06 01:06:13

DNA findings point to royal roots in African nations

Holland attributes his success to GeneTree, a Utah-based DNA testing service that came up with the Cameroon connection. But GeneTree's chief scientific officer, geneticist Scott Woodward, said Williams' case was far from unusual. "This isn't our home run," Woodward told me. "It takes a lot of regular work. But what did do was give us some nice clues and hints about where he should concentrate his efforts. Should he be looking in Cameroon, or should he be looking in Nigeria? That makes a big difference." In fact, previous genetic tests had indeed suggested that Holland's [more...]

Date: 2010-09-09 14:14:55

PLoS ONE: Evidence of Authentic DNA from Danish Viking Age ...

Linea Melchior1, Toomas Kivisild2, Niels Lynnerup3, Jørgen Dissing1*1 Research Laboratory, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark, 2 Leverhulme Center for Human Evolutionary Studies, The Henry Wellcome Building, University of Cambridge, Cambridge, United Kingdom, 3 Laboratory of Biological Anthropology, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark Abstract Top Background Given the relative abundance of modern human DNA and the inherent impossibility for incontestable proof of authenticity, results obtained on ancient [more...]

Date: 2008-05-28 07:00:00

Forensic Serology Information Page

What is Forensic Serology?According to forensic serologist Marcella Jones of www.ForensicMentor.com, forensic serology is the analysis of body fluids as they relate to forensic cases, including DNA analysis. Accordingly, the role of the forensic serologist involves:Examining evidence for the presence of body fluids e.g. blood, semen, hair, tissue, saliva, feces, and urineEvaluating evidence for potential DNA analysisEvaluating species of body fluidsPerform PCR (polymerase chain reaction) based typing of STR (short tandem repeat sequences) of genomic DNA, or mitochondrial sequence analysis of [more...]

Date: 2010-02-04 08:00:00

Low Citrulline in Leigh Disease: Still a Biomarker of Maternally ...

Muscle and liver biopsies were considered for respiratory chain studies, but because of hypocitrullinemia, molecular analysis for maternally inherited Leigh syndrome was first performed, revealing in both siblings the mitochondrial DNA T8993G mutation (95% heteroplasmy), allowing to avoid tissue biopsies. Hypocitrullinemia, an occasional finding in mitochondrial diseases, has been specifically associated with T8993G mutation. However, only few patients have been reported, and the prevalence of hypocitrullinemia in 8993 mitochondrial DNA mutations is unknown. In a small series of 16 Leigh [more...]

Date: 2010-07-14 17:24:17

Forensic DNA Analysis – What is Mitochondrial DNA?

Mitochondrial DNA can also be found in some tissues where nuclear DNA normally does not exist. For instance, dead cellular debris can be found in human hair. However, the only thing that is living in human hair the follicle, [more...]

Date: 2010-08-22 08:31:49

Case Analyst (Salt Lake City)

Successful candidate will perform genotyping analysis. Experience using GeneMapper genotyping software a plus. Experience performing DNA extraction, PCR and genotyping for STR analysis and mitochondrial sequencing essential. Other lab duties as directed. Required: • BS in Biochemistry, Molecular Biology, Biology or related field • 1 year experience in laboratory • Knowledge of molecular biology testing, analysis and reporting. • Must be able to use hands, stand for periods of time and lift up to 20 lbs. Preferred: • Computer and Microsoft applications proficiency • 2 years [more...]

Date: 2010-10-01 04:43:12

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