Al Fin: A Hint of Epigenetics
We are learning that the genetic code is only the short, first chapter in a very long book. The non-genetic "code" -- the non-coding DNA and RNA -- has a logic all its own. We are just beginning to decrypt the obscure cipher. The possibilities for the transformation of life as we know it seem just as grandiose as were the hopes of the early promoters of the human genome enterprise. Only this time, we are working at a deeper level of sophistication. How many more levels will we need to descend before we reach the promised land of genetic medicine? ... some 95 or 98 percent of human DNA was [more...]
Date: 2010-10-13 15:07:00
Thank You, Walgreens, for Nixing That Off-the-Shelf DNA Test | BNET
After some prodding from the FDA, Walgreens (WAG) will not sell a spit-and-send DNA test that assesses a person’s chances of becoming obese, developing psoriasis, going blind, or having a baby with cystic fibrosis, Tay-Sachs and other genetic disorders. The move snuffs out the national freakout that would have occurred had this product gotten onto store shelves. Usually, it’s good for consumers to have access to cheap, reliable medical devices that give them more information about their health. (If you’ve ever gotten one of those bills from Quest Diagnostics or another test lab, [more...]
Date: 2010-05-13 14:50:12
Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...
RAPD-PCR mixture was set up that contained 20-50 ng of genomic DNA, 40 pmol of primer, 1 U of Taq polymerase (Bangalore GENEI.), 200 uM (each) deoxynucleoside triphosphate, 10 mM Tris-Cl (pH 8.3), 50 mM KCl, 3 mM MgCl2 and 1% DMSO. Each reaction mixture was overlaid with 25 ul of mineral oil and amplified with a Perkin-Elmer Cetus DNA Thermal Cycler model TC-1 as follows: (i) Initial denaturation, 1 cycle consisting of 5 min at 94oC and (ii) 35 cycles, with 1 cycle consisting of 1 min at 94oC, 2 min at 36oC, and 3 min at 72oC, followed by a final extension step at 72oC for 10 min. RAPD [more...]
Date: 2009-09-25 16:32:00
The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...
There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]
Date: 2010-10-04 21:15:46
Blog: Behavior Ecology And Sociobiology » Plant Molecular Biology ...
Plant Cell Reports 2001 , 20:731-737. Publisher Full Text Olhoft P, Somers D: L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Reports 2001 , 20:706-711. Publisher Full Text Trick HN, Finer JJ: Sonication-assisted Agrobacterium-mediated transformation of soybean embryogenic suspension culture tissue. Plant Cell Reports 1998 , 17:482-488. Publisher Full Text de Oliveira MLP, Febres VJ, Costa MGC, Moore GA, Otoni WC: High-efficiency Agrobacterium-mediated transformation of citrus via sonication and [more...]
Date: 2003-08-01 07:00:00
Upcoming Next-Gen Sequencing Conferences | RNA-Seq Blog
NeXt-GENeration sequencing platforms create sequence reads of DNA fragments for genome variation studies, RNA for transcriptome studies, DNA-protein interactions for epigenetic studies, and chromosomal DNA of large genome nucleotide variations for copy number studies. The X-Gen Congress and Expo is uniquely designed to facilitate the cross fertilization of established and emerging genomic technologies, along with exciting applications. In addition, you will learn why data is the driving force that enables genomic discoveries. Next-Gen Sequencing Congress April 26th – 27th, 2011 [more...]
Date: 2010-09-29 15:13:14
ERV's latest blog on the FDA paper. One step too far?.
PNAS paper, using standard PCR to ID 'positives' and 'negatives', with insane numbers of bands as a result of non-specific primer binding. Insane numbers. As if this is 1990 and we dont know what 'Real Time PCR' and 'Taqman Probes' are. Im going to ignore that and grant the premise they really have IDed viral sequences. And they arent XMRV. They dont cluster anywhere near XMRV on a phylogenetic tree. Theyre something else entirely. Okay, well, logically it could be contamination from mouse DNA. So they checked for that... by looking for mouse mitochondrial DNA. *blink* I understand [more...]
Date: 2010-08-23 07:00:00
The Freshman Perspective
DNA fingerprinting and maps of the human genome have always existed. 21. Woody Allen, whose heart has wanted what it wanted, has always been with Soon-Yi Previn. 22. Cross-burning has always been deemed protected speech. 23. Leasing has always allowed the folks to upgrade their tastes in cars. 24. “Cop Killer” by rapper Ice-T has never been available on a recording. 25. Leno and Letterman have always been trading insults on opposing networks. 26. Unless they found one in their grandparents’ closet, they have never seen a carousel of Kodachrome slides. 27. Computers have never [more...]
Date: 2010-10-08 01:24:00
Australian Aborigines Were Once Indians - Study
Dr Raghavendra Rao worked with a team of researchers from the Anthropological Survey of India to sequence 966 complete mitochondrial DNA genomes from Indian 'relic populations'. He said, "Mitochondrial DNA is inherited only from the mother and so allows us to accurately trace ancestry. We found certain mutations in the DNA sequences of the Indian tribes we sampled that are specific to Australian Aborigines. This shared ancestry suggests that the Aborigine population migrated to Australia via the so-called 'Southern Route'." The 'Southern Route' dispersal of modern humans suggests movement of [more...]
Date: 2010-10-08 03:08:00
FamilyTreeDNA: 6th International Conference 30-31 Oct Houston
HOUSTON, (August 11, 2010) -- Family Tree DNA, the world leader in genetic genealogy, will host its 6th International Conference on Genetic Genealogy on October 30-31, 2010, at the Sheraton North Houston in Houston, Texas. Each year, world renowned experts in genetics and science present cutting-edge developments and exciting new applications at this two-day educational forum which draws attendees from Family Tree DNA's Group Administrators from around the world. This year's conference will focus on the new Family Finder test which allows customers to find relatives across all ancestral [more...]
Date: 2010-08-11 23:17:00
FTDNA Family Finder test
(BUSINESS WIRE)--Family Tree DNA, the first and largest genealogical DNA testing company, today announced the launch of the Family Finder DNA test for connecting family members across all ancestral lines. The test utilizes Affymetrix’ recently launched Axiom™ genotyping technology and the GeneTitan® System to confidently match a wide range of family relationships within five generations. Family Finder represents a major advancement over earlier genealogical tests, which revealed only connections along specific paternal lines (for males) or maternal lines (males or females). [more...]
Date: 2010-05-15 07:13:00
Eastman's Online Genealogy Newsletter: Family Tree DNA Announces ...
The following announcement was written by Family Tree DNA: HOUSTON, (August 11, 2010) -- Family Tree DNA, the world leader in genetic genealogy, will host its 6th International Conference on Genetic Genealogy on October 30-31, 2010, at the Sheraton North Houston in Houston, Texas. Each year, world renowned experts in genetics and science present cutting-edge developments and exciting new applications at this two-day educational forum which draws attendees from Family Tree DNA's Group Administrators from around the world. This year's conference will focus on the new Family Finder test [more...]
Date: 2010-08-11 23:29:44
Epigenetics Papers: DNA Methylation Analysis by MethyLight Technology
Authors discussed each step for this procedure: (1) determining the site of interest for methylation analysis; (2) methylation-specific primers and fluorogenic probes design; (3) genomic DNA isolation; (4) bisulfite conversion; (5) real- time methylation analysis; and (6) data processing. For primer design, some complications particular to the bisulfite modification should be taken into account: All unmethylated cytosine residues in the genome are converted this reduction in genomic complexity reduces the annealing specificity of PCR primers and fluorogenic probes, which not only [more...]
Date: 2007-08-19 01:26:00
Thrombosis Journal | Full text | Effect of PlA1/A2 glycoprotein ...
Genomic DNA was prepared from peripheral lymphocytes by the salt precipitation method . The PlA1/A2 alleles of the GP IIIa gene were identified on the basis of MspI enzyme site restriction analysis after amplification of a 476 base pairs GP IIIa fragment (sens amorce 5'-ATA-AGC-TTA-GCT-ATT-GGG-AAG-TGG-TAG-GGC-CTG-3', antisens amorce 5'-CTT-CTG-ACT-CAA-GTC-CTA-ACG-3'). The GP IIIa gene was amplified using the polymerase chain reaction (PCR) method. Each amplification product was verified on an agarose gel. Amplification results in a 476 base pairs (bp) fragment. Digestion was obtained with [more...]
Date: 2008-01-15 08:00:00
File type:PDF – Download PDF Reader Higher yields from genomic templates with REDTaq Genomic DNA Genomic or large construct DNA containing target sequence. is digested with a restriction . Genomic DNA Purification Instructor's Manual [more...]
Date: 2010-06-23 09:16:14
Mitochondrial DNA Genealogy
The mitochondrial DNA genealogy is the study of a person’s mother’s family line of maternal ancestors. This DNA is passed down from mother to each of her children exactly as she has it, with no mutations, except in the very rare instances. Because of the traditions in our society of the wife taking her husband’s last name, a surname genealogy trace of the female line of the family tree is more difficult. On the flip side, since this DNA strand is more stable than the Y DNA, more conclusive results can be determined for the testing of it. The mt-DNA is sometimes referred to as the Full [more...]
Date: 2010-10-05 09:25:26
Can Making Science Fun Woo The Next Generation?
Extracting DNA to Make Goo Resembling Strawberry Snot – The National Human Genome Research Institute worked with students to extract DNA from strawberries using a dish soap, table salt, rubbing alcohol, a coffee filter and a plastic bag. The red DNA the students found was drippy and the texture of what comes out of their noses. Students went home with details about the experiment so they could conduct it with family and friends. What’s Green and Good and What’s Poisonous? EPA’s father of green chemistry, Paul Anastas, Ph.D., clad in a white lab coat, led a roundtable of students who [more...]
Date: 2010-10-06 14:04:39
Isolation and characterization of PR1 homolog from the genomic DNA ...
Southern hybridization with genomic DNA digests was carried out to check its genomic organization. The induction of this gene was observed in the somatic embryos treated with salicylic acid, thereby implying its possible involvement during systemic acquired resistance. View Entire [more...]
Date: 2010-10-03 10:56:44
UC San Diego to Lead Personalized Medicine Project on Mood-Stabilizer
The other participating institutions are Indiana University, University of Chicago, University of Iowa, University of Pennsylvania, Johns Hopkins University, University of Michigan, the Translational Genomics Institute in Phoenix, Case Western Reserve University, University of Bergen in Norway, and Dalhouise University in Canada. Research plans call for diagnosing and treating with lithium a total of 700 patients at the ten sites, following the patients’ progress for two years, with a particular emphasis on noting the occurrence of any relapses and the period of time it takes to recover. [more...]
Date: 2010-09-07 17:08:00
Mitochondrial Disease can be diagnosed with a new test.
The SOLiD NextGen sequencing platform developed by Life Technologies was used for testing because of its low error rate and high throughput. This rigorous sequencing of mitochondrial DNA by MitoDx can detect mixtures (heteroplasmy) of normal and mutant DNA even when the mutant or normal form is present at very low levels. This allows mitochondrial disease to be evaluated much more sensitively in an accessible tissue like blood or saliva. Richard Boles, MD, the Director of the Mitochondrial and Metabolic Disorders Clinic at Childrens Hospital Los Angeles says, “MitoDx has confirmed the [more...]
Date: 2010-09-29 04:56:00
DNA: Size Does Matter. But is it Science or Art? « Exploratorium ...
Then I randomly found this website, which starts with the same idea as our DNA Cheek Cell Extraction Demo but takes it to a whole new level: You send your cheek cells in the mail to their company. They extract your DNA from your cells, then use PCR to amplify unique sections of DNA in your genome. This DNA is loaded into a gel and a current is run through the gel to separate the DNA based on size. UV dye is added to the whole thing to make your DNA strands visible, a photograph is taken, and voila!…a giant canvas print with the color scheme of your choice is ready to hang on your [more...]
Date: 2008-05-14 07:37:47
Learn The Basic Process Of DNA Paternity Testing
Technology has finished so many for many of us – in actuality it has helped us strike many of the shortcomings in life. It certainly has done things the lot easier as well as many faster to accomplish. Technology, indeed, has facilitated lots of discoveries creation hold up reduction of the consternation as well as has aided information exchnage even to distant flung tools of the world.The area of healing scholarship is the single of the areas which has severely benefited from technological development. One of the many appropriate contributions of scholarship in the healing margin is [more...]
Date: 2010-10-09 04:16:22
Tech Support Scientist,Bioinformatics (West,Central or Eastern ...
This role will be responsible for troubleshooting PacBio SMRT DNA sequencing data employing skills in bioinformatics, data analysis, biological insight and software engineering. Provide timely technical and application assistance in response to customer inquires. Resolve bioinformatics issues escalated from Field Applications Specialists or customers. Issues will be received via phone, email, Web and Live Chat. Contribute to the ongoing development of our product Knowledge Base to capture lessons learned and solutions to cases. Collaborate with Product Marketing, [more...]
Date: 2010-08-27 16:37:28
Orange Mountain Bike » DNA sequencing
Pages About Recent Posts Sofa Tables – Appreciate the Designer Post Chairs Buying an Post President Online MIrra Chairmen Crucial Erect Circle Reckoned Features Benefits of Ergonomic Computer Chairmen Links WordPress.com WordPress.org Archives(Yearly) 2010 Archives(Monthly) October 2010 September 2010 August 2010 March 2010 Archives(Daily) October 13, 2010 October 12, 2010 October 11, 2010 October 7, 2010 October 5, 2010 September 26, 2010 September 16, 2010 September 13, 2010 September 5, 2010 August 21, 2010 March 8, 2010 Knowledge of DNA sequences has got indispensable for basic [more...]
Date: 2010-10-12 16:41:05
race/history/evolution notes: Highly divergent ancient hominin ...
Svante Paabo assumes "some new creature" "carried this mitochondrial genome out of Africa about a million years", as do most commentators. It strikes me that a multiregionalist explanation is as consistent if not more consistent with the evidence at hand. If we entertain the possibility that "African Eve" ultimately descends from Eurasian Homo erectus, we don't need to posit some unknown intermediate "out of Africa" event. Reference: Johannes Krause, Qiaomei Fu, Jeffrey M. Good, Bence Viola, Michael V. Shunkov, Anatoli P. Derevianko & Svante Pääbo. The complete mitochondrial DNA genome of [more...]
Date: 2010-03-25 03:22:00
Family Tree DNA to Host Their 6th International Conference on ...
designated DNA testing company for their Genographic Project, a history-making study of the migrations of mankind. To date, the company has processed more than 300,000 Genographic Project DNA tests. Family Tree DNA’s own laboratory-the Genomics Research Center-participated in the Genographic Project’s first published paper and other scientific papers. Offering the most popular and wide-ranging DNA-testing service in the field of genetic genealogy, Family Tree DNA prides itself on its commitment to the practice of solid, ethical science. Since its beginnings, the company has associated [more...]
Date: 2010-08-12 00:47:09
csirhrdg.res.in | CSIR UGC NET 2011 Life Science Syllabus 2011 ...
Analysis of RNA, DNA and proteins by one and two dimensional gel electrophoresis, Isoelectric focusing gels. Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems. Expression of recombinant proteins using bacterial, animal and plant vectors. Isolation of specific nucleic acid sequences Generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors. In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms. Protein sequencing methods, detection of post translation modification of proteins. DNA [more...]
Date: 2008-06-21 07:00:00
Complete Genomics 2010 IPO | InvestorPlace
Complete Genomics Inc. plans to raise up to $86.2 million in an initial public offering to help fund improvements and expansion of its DNA sequencing technology. Complete Genomics will join the Nasdaq exchange after its 2010 IPO, and will trade under the ticker GNOM after the stock offering. The Mountain View, Calif., company said in its SEC registration statement that it has developed and commercialized “an innovative DNA sequencing platform that we believe will become the preferred solution for complete human genome sequencing and analysis.” Price is key for its assembly-line [more...]
Date: 2010-08-06 15:43:34
New study shows that human and chimpanzee DNA are very different ...
The whole chimp DNA thing is an example of reporting bias. They like to use the “98% similar” argument. But there are 3 billion letters in the human DNA genome, and they are 98.5% similar with chimps. DNA is fantastically complex, and the math yields 45 million differences. Most of the “science” in the article was based on the unproven assumption that chimps and humans have a common ancestor. The “rapid evolution” argument is a just-so story about how the DNA could have tens of millions of changes in only a few million years (according to their theory), as well as how it kept [more...]
Date: 2010-04-30 20:00:48
Genomic DNA Extraction » DNA Extraction Protocol Video
Video showing how DNA Extraction protocol should be conducted. It shows how we can extract dna from different organisms. If you want to learn about Genomic DNA Extraction and more about DNA Purifcation you should watch this video. All you need is DNA Extraction Kit. In School or College Lab this DNA Kit is ussually easy to find. Filed under: Genomic DNA Extraction by [more...]
Date: 2010-03-04 08:17:21
Paternity Testing - Are You Raising Someone Else's Child? - Hackerwap
Even without a sample from the mother, DNA paternity test results are up to 99.9999% accurate'that's one-in-a-million odds your results are incorrect. Most companies provide a free home kit for you to provide the samples and require you to send the kit back to the laboratory with the accompanying fee. Because many companies are aware of the discomfort of drawing blood from a child in order to get a sample, buccal (mouth) swabs are being accepted as an alternative. By gently massaging the inside of the child's mouth, cheek cells are collected. These cells are then sent to the lab for testing. [more...]
Date: 2005-02-15 08:00:00
Thrombosis Journal | Full text | Expression of sterol regulatory ...
DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]
Date: 2009-02-18 08:00:00
23andMe's DNA Mixup Leaves 96 Customers With Wrong Test Results
23andMe's DNA Mixup Leaves 96 Customers With Wrong Test Results by Amar Toor on June 8, 2010 at 12:45 PM As we know all too well here at Switched, everyone makes mistakes. Not everyone, however, makes mistakes quite as far-reaching as was the blunder that private DNA-testing company 23andMe recently made. On Friday, the company announced that "a number of new 23andMe customer samples were incorrectly processed" by the third-party lab that conducts the DNA tests, and confessed that "up to" 96 people received test results that weren't really theirs. As you can imagine, mass confusion ensued. [more...]
Date: 2010-06-08 17:45:00
Welcome to Sage
Synthetic genomics is the chemical synthesis of DNA sequences. The synthesized sequences can already exist – as in the chemical synthesis of genes naturally found in an organism – or it can involve the synthesis of novel, unnatural DNA sequences. Synthetic biology is both the design and construction of new biological parts, devices, and systems and the redesign of existing natural biological systems for useful purposes. Because the synthetic life sciences may enable the synthesis of biomolecules, whole genomes, and even simple life forms, these sciences have enormous potential, as they [more...]
Date: 2010-09-16 13:18:45
Popular Mechanics Posts 2010 BREAKTHROUGH Awards — The Gadgeteer
While working at the National Institutes of Health in the early 1990s, Venter developed a way to rapidly discover genes by exploiting snippets of DNA called expressed sequence tags. In 1992, he founded The Institute for Genomic Research, and, three years later, he and a team decoded the first genome of a free-living organism. That led to Venter’s best-known breakthrough, mapping the human genome. Last May, he achieved a defining moment in the history of biology when he inserted digitally created DNA into a living bacterium, forming the first synthetic life. His ultimate goal is to design [more...]
Date: 2010-09-28 20:43:25
A GMO primer — What you should know about genetically modified ...
Ninety-five percent of the sugar-beet crop in the U.S. is genetically modified, but a recent federal ruling may have a huge impact on that. When it all began: The technologies that led to genetically modified foods were developed in the 1970s, with the advent of recombinant DNA technology. At the time, there were a lot of ethical debates and concerns about crossing the species barrier and transgenic technology. The focus then was on bacteria — mainly E. coli — and putting genes from different species into bacteria. Big Business: In the 1980s, Monsanto pushed to develop and use GM [more...]
Date: 2010-10-12 16:28:00
Senior Software Engineer - SEQanswers
Complete Genomics is an emerging DNA sequencing technology with the promise of high throughput, low cost sequencing of human genomes. The technology has proven itself by sequencing of hundreds of human genomes for customers from research institutions to pharmaceutical giants to clinical researchers. Complete Genomics is aggressively pursuing innovations to aid its customers in the study of human disease and to push its service into vast new markets. It has filed an S1 for IPO. The assembly pipeline team is an amalgam of exceptional bioinformaticians and software engineers of diverse [more...]
Date: 2010-10-01 18:28:02
How is a DNA microarray different that gel electrophoresis?
I know that they are both used to kind of sort DNA and whatnot, but what’s different? Best answer: Answer by _haze_ A DNA microarray (also commonly known as gene or genome chip, DNA chip, or gene array) is a collection of microscopic DNA spots, commonly representing single genes, arrayed on a solid surface by covalent attachment to a chemical matrix. DNA arrays are different from other types of microarray only in that they either measure DNA or use DNA as part of its detection system. Qualitative or quantitative measurements with DNA microarrays utilize the selective nature of DNA-DNA or [more...]
Date: 2010-07-29 20:16:32
Eppendorf to market Akonni TruTip™ « Frederick County Biotech ...
HAUPPAUGE, N.Y. & FREDERICK, Md.–(BUSINESS WIRE)–Eppendorf North America and Akonni Biosystems today announced they have entered into a joint marketing agreement to promote Akonni TruTip nucleic acid extraction kits configured for use with Eppendorf epMotion automated pipetting systems. Under the agreement, Eppendorf will promote the extraction kits to clinical, clinical research and forensic laboratories in North America – providing users with access to the industry’s most rapid and reliable means to automatically extract PCR-ready DNA and/or RNA from larger volume [more...]
Date: 2010-04-26 16:36:03
Walgreens halts genetic test sales on FDA concerns
Walgreens is holding off on selling the tests until the FDA's concerns are resolved, media reports say. An FDA spokesperson could not be reached. The test kits made by Pathway Genomics shows the market for DNA testing is expanding beyond settling the question of whether the mother on the "Maury Show" is telling the truth about the identity of her child's father. People also examine their DNA to learn about their ancestry. Scientists at the National Geographic Society are collecting DNA samples from people around the world to learn about the origins of man. Genetic tests like the one offered [more...]
Date: 2010-05-13 22:00:00
Evolution News & Views: Time Aping over Human-Chimp Genetic ...
Darwinists often cite similarities in non-coding DNA as evidence of chimp-human common ancestry. Yet the Time article explains that non-coding DNA has function--perhaps holding the functions responsible for the differences between humans and chimps: Those molecular switches lie in the noncoding regions of the genome--once known dismissively as junk DNA but lately rechristened the dark matter of the genome. ... "But it may be the dark matter that governs a lot of what we actually see." Though the article still asserts much of the genome is junk, Richard Sternberg and James A. Shapiro wrote [more...]
Date: 2006-10-04 18:02:26
Technology Review: A Family Mystery, Solved by a Genome
More than a dozen people who have had their genomes sequenced stand on stage in an R&D center near Boston. Billed as the last time all such people might fit in one room before the technology moves into the mainstream, the event doesn't quite include the whole group: actress Glenn Close and South African archbishop Desmond Tutu, among others, didn't make it. But those who did include James Watson, codiscoverer of the structure of DNA; Harvard historian Henry Louis Gates Jr.; entrepreneur Esther Dyson; and a smattering of leaders from gene-sequencing companies. Leaning against a wall at one end [more...]
Date: 2010-08-25 00:00:00
Free molecular biology software for Macs | Bioinformatics Blog
Posted on the October 12th, 2010 under Free mac software by bioinformatics-blog 1. Serial Cloner Serial Cloner is fantastic all-in-one workbench; import and manipulate sequences, construct plasmid and restriction site maps, determine %GC and fragment TM, extract and ligate fragments, perform virtual PCR… and lots more, all in one window using a very intuitive graphical interface. 2. 4Peaks 4Peaks is an extremely user friendly DNA sequence chromatogram viewer and editor from the extremely talented guys at Mekentosj. It’s miles better than any of it’s clunky counterparts… try it, [more...]
Date: 2010-10-12 11:08:47
Biotechnology, XII: Model Paper « Home works and Assignments Online
Which vectors are used in human genome sequencing & why? Explain the blue white method of screening the recombinant plasmid? A single cell of E.coli is supposed to produce about 2000 different proteins. under optimum conditions. One of the desired intercellular enzymes produced is in the form of 2500 molecules/cells. If the molecular weight of that enzyme is 100000D.How many E.coli cells are required to produce 1gm of enzymes. What are secondary metabolites? Name the secondary metabolites produced by the following plants. (a)Atropa beladona (b) Cinchona officinalis. SECTION C (3 marks [more...]
Date: 2010-10-13 05:07:00
Mother of All Humans Lived 6000 Years Ago
Further, for so few--only 21.6 nucleotides out of 16,569--DNA differences to have accumulated at anything near the measured mutation rates, a much shorter time than "200,000 years" must have transpired since Mitochondrial Eve arrived on the scene. To stretch out across evolutionary time the occurrences of so few DNA changes requires a gymnastic juggling of the coefficients used in the various models, and appears to require a biologically unrealistic, super-slow mutation rate. Making the data fit vast timescales requires the use of a broken, circular-reasoning-based, evolutionary [more...]
Date: 2010-09-07 08:00:00
PLANT TISSUE CULTURE TECHNIQUE AND PCR TECHNOLOGY
SSR assays require a minimal amount of genomic DNA. Most of the crop improvement programs are confined to evaluation and selection of naturally occurring clonal variations. In vitro culture techniques provide an alternative means of plant propagation and an important tool for crop improvement. The in vitro cultures are also of low risk for genetic variation since it is more resistant to genetic changes while occurrence of cell division in vitro condition. Gel electrophoresis is an important molecular biology tool which enables us to study DNA. It can be used to determine the sequence of [more...]
Date: 2010-09-08 04:07:38
Nano Patents and Innovations: DNA Fingerprinting Pioneer Discovers ...
Nano Patents and Innovations is dedicated to nanotechnology news, patents, markets, products and research innovations One of the key drivers of human evolution and diversity, accounting for changes that occur between different generations of people, is explained by new research published September 5 by world-renowned scientist Professor Sir Alec Jeffreys, who discovered DNA fingerprinting at the University of Leicester. Professor Jeffreys has spent over two decades since his landmark discovery in 1984 investigating what he describes as "pretty bizarre bits of DNA" - highly variable repeated [more...]
Date: 2010-09-06 11:27:00
My faith is falsifiable, Professor Coyne. Is yours? | Uncommon Descent
The creation of a machine that was capable of conversing at length about any topic – including its own mental states and life story – in such a way that it could fool an audience of intelligent people into thinking that it was human. Observations that would cause me to abandon belief in Intelligent Design 1. An empirical or mathematical demonstration that the probability of the emergence of life on Earth during the past four billion years as a result of purely natural processes, without any intelligent guidance and starting from a random assortment of organic chemicals, is greater than [more...]
Date: 2010-10-13 00:36:41
CN Tower to Double as DNA Barcode
25 to look like the world's biggest DNA barcode as part of the official launch of the University of Guelph-based International Barcode of Life Project (iBOL). iBOL is the world’s largest biodiversity genomics initiative aimed at creating a digital identification system of all life on Earth using DNA barcoding. The international project will be launched by Glen Murray, Ontario’s minister of research and innovation, at the CN Tower at 6:30 p.m. University of Guelph president Alastair Summerlee and iBOI scientific director Paul Hebert, a professor in the University’s Department of [more...]
Date: 2010-09-24 13:58:11
تقنية البلمرة الجزيئية » تعرف عن عملية استخلاص شريط الحمض النووي ...
The VX Viral DNA/RNA Purification CorProtocol™ 25101 allows for walk-away automated preparation of low copy viral DNA/RNA from low total nucleic acid-yielding samples. Extracted viral DNA/RNA is of high quality and suitable for a wide variety of downstream applications. The default sample volume is 200 µL but can be scaled up to 500 µL with the use of adjustments to this protocol coupled with an alternative run file ( VX_RNA_DNA_CorProtocol_250_500ul_25101_06.CAS4 ) on the instrument. Increased volumes of certain reagents are required for the extraction of sample volumes greater [more...]
Date: 2010-07-09 16:20:26