File type:PDF – Download PDF Reader Higher yields from genomic templates with REDTaq Genomic DNA Genomic or large construct DNA containing target sequence. is digested with a restriction . Genomic DNA Purification Instructor's Manual [more...]
Date: 2010-06-23 09:16:14
Quick and reliable DNA purification and sequencing
Keep up with the latest research, events and funding news. Add the observer, dissemination and funding widget to your website or blog The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems. Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting Station, a high-speed precision pipettor. With its versatile shelf configuration options, the [more...]
Date: 2010-07-28 13:03:49
تقنية البلمرة الجزيئية » تعرف عن عملية استخلاص شريط الحمض النووي ...
The VX Viral DNA/RNA Purification CorProtocol™ 25101 allows for walk-away automated preparation of low copy viral DNA/RNA from low total nucleic acid-yielding samples. Extracted viral DNA/RNA is of high quality and suitable for a wide variety of downstream applications. The default sample volume is 200 µL but can be scaled up to 500 µL with the use of adjustments to this protocol coupled with an alternative run file ( VX_RNA_DNA_CorProtocol_250_500ul_25101_06.CAS4 ) on the instrument. Increased volumes of certain reagents are required for the extraction of sample volumes greater [more...]
Date: 2010-07-09 16:20:26
IDT enters exclusive agreement with 454 Life Sciences to provide ...
IDT enters exclusive agreement with 454 Life Sciences to provide custom primers for GS FLX Titanium Chemistry 4 October 2010 – HPLC purified and MS certified primers for genome sequencing CORALVILLE, IA: Integrated DNA Technologies (IDT), the custom biology company, has signed an exclusive agreement with 454 Life Sciences, a Roche company, to provide researchers with custom primers for the GS FLX Titanium Chemistry, used on the company’s GS FLX System and GS Junior System. Available as FusionPrimers or Rapid Library MID (Molecular Identification) Adaptor Oligos, these products are used [more...]
Date: 2010-10-04 16:16:19
Cytoplasmic & Nuclear RNA Purification Kit
RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]
Date: 2010-08-16 04:58:00
NALP1 in Vitiligo-Associated Multiple Autoimmune Disease — NEJM
DNA was prepared from peripheral-blood specimens with the use of a genomic DNA purification kit (Puregene, Gentra Systems) or from saliva specimens with the use of a DNA self-collection kit (Oragene, DNA Genotek). [more...]
Date: 2007-03-22 05:00:00
Bio Saga Blog: 5-Day Hands-on Workshop on Molecular Biotechnology ...
Recombinant DNA Technology: Cutting & pasting of DNA molecules (i.e. restriction digestion, ligation, DNA gel eectrophoresis, and gel extraction.) Bioinfo part: Bioinformatics of DNA database / SequenceAnalysis: Pairwise sequence alignment, Multiple sequence alignment, Pattern search.Tools: BLAST, CLUSTALW/X, CLC Bio Main Workbench Genetic Engineering: Transformation and plasmid purification, cloning and sub-cloning, amplification of DNA fragments by polymerase chain reaction (PCR). Bioinfo part: Designing of PCR primers In-silico cloning Searching for homologous and paralogous [more...]
Date: 2010-09-29 05:41:00
(IUCr) Expression, purification and crystallization of Chaetomium ...
The copy number of the gene integration in the genome of Pichia pastoris was determined to be between 7 and 12. PCR amplifications were carried out based on genomic DNA using primers corresponding to [more...]
Date: 2010-08-28 07:00:00
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
The story of "Fat" and "Stubborn"
3- Transformation yields colonies, but upon DNA purification I can not confirm the presence of the insert into the vector. Blue white cloning results in the usual blue and white colonies to aid selection, so I guess ligation would work. Restriction analysis gives inconclusive results, as weak bands can sometimes be seen at the right sizes, but there are other unexplained bands there too, that can not be explained by incomplete digesion. Checking just the sizes of the uncut vectors shows the recombinant vector often running at a faster rate than the empty vector! ( see below for my theories) [more...]
Date: 2010-08-17 21:23:00
Relating underrepresented genomic DNA patterns and tiRNAs: the ...
© 2010 Cserzo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. AbstractBackground One of the central problems of post-genomic biology is the understanding of regulatory network of genes. Traditionally the problem is approached from the protein-DNA interaction perspective. In recent years various types of noncoding RNAs appeared on the [more...]
Date: 2010-09-22 00:00:00
Applied DNA Sciences' Botanical SigNature DNA Convicts Thieves in ...
SigNature DNA is a botanical mark used to authenticate products in a unique manner that essentially cannot be copied. APDN also provides BioMaterial GenoTyping™ by detecting genomic DNA in natural materials to authenticate finished products. Both technologies protect brands and products in a wide range of industries and provide a forensic chain of evidence that can be used to prosecute perpetrators. To learn more, go to www.adnas.com where APDN routinely posts all press releases. The statements made by APDN may be forward-looking in nature and are made pursuant to the safe harbor provisions [more...]
Date: 2010-10-08 15:16:13
An ELISA-based high throughput protein truncation test for ...
Here, we report the next generation HTS-PTT using triple-epitope-tagged proteins and demonstrate, for the first time, its efficacy on clinical genomic DNA samples for BRCA1/2 analysis. Methods: Segments of exons 11 of BRCA1/2 open reading frames were PCR amplified from either blood derived genomic DNA or cell line mRNA. PCR primers incorporate elements for cell-free transcription/translation and epitope tagging. Cell-free expressed nascent proteins are then antibody-captured onto the wells of a microtiter plate and the relative amount of truncated polypeptide measured using antibodies against [more...]
Date: 2010-10-04 16:47:13
Is there a different type of primer i have to use when running PCR ...
Question by bob oso: Is there a different type of primer i have to use when running PCR with genomic DNA compared to cDNA? Im still in school but when i run a PCR, I normally isolate RNA first then RT to cDNA and run PCR with that. This time i isolated the genomic DNA from the sample and ran PCR using the beta-actin primer i always used. Dimers were faintly visible on my gel but nothing really came up like it normally would have or should have. I was told that when running PCR with genomic DNA, a different kind of primer had to be used; one that is specific to genomic DNA. Im confused as to [more...]
Date: 2010-10-01 19:54:46
Quick & Reliable DNA Purification and Sequencing…
BioCel DNA Purification and Sequencing Royston, UK -- An applications bulletin is available from Agilent Automation Solutions that describes an automated system that provides for quick and reliable DNA purification and sequencing. The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems. Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting [more...]
Date: 2010-08-14 14:00:00