Our blogs and videos choice: dna template

Heliscope builds world's fastest DNA sequencer | Singularity Hub

The Short: Technology Review reports that for just $1,350,000 USD you can have have the worlds fastest commercially available DNA sequencer, called the Heliscope. The machine developed by Helicos BioSciences takes just one hour to read 1.3 billion base pairs from a strand of DNA. Here is a picture of the beauty: HeliScope™ Single Molecule Sequencer The Long: The Heliscope is being marketed as a DNA microscope. It is unique in the field of DNA sequencing because it sequences an actual DNA strand whereas most other sequencing technologies use PCR to create millions of copies of an original [more...]

Date: 2008-07-14 16:58:33

Blog posts (52) | Videos (13)
 


What characteristics of DNA polymerase III make it an ideal enzyme ...

The key features of DNA polymerase III are its catalytic potency, its fidelity, and its processivity. It is able to catalyze the addition of ~103 bases per second, compared to only about 10 per second for DNA polymerase I,with an error frequency of about 1:104–105. One of the unique features of DNA polymerase III is its ability to continuously synthesize very long (thousands of bases) stretches of DNA, unlike DNA polymerase I. This is a consequence of its processivity; the β2 sliding clamps ensure that it remains bound to the template strand DNA. In contrast, DNA polymerase I, without a [more...]

Date: 2010-03-07 11:59:00


Error-Prone DNA Repair Activity during Somatic Hypermutation in ...

Duplications and deletions were associated with N additions, suggesting participation of a DNA polymerase with some degree of template independence during the repair of DNA breaks initiated by activation-induced cytidine deaminase. Other mutations were consistent with some in vitro activities of mammalian translesion DNA polymerase η: tandem base substitutions, strand slippage, and small insertions/deletions. The nature of substitution patterns shows that DNA lesions at shark Ig genes recruit DNA repair factors with a species-specific repertoire of activities. We speculate that the tandem [more...]

Date: 2010-10-07 06:53:59


[Dna: Replication, Repair, Recombination, and Chromosome Dynamics ...

In most cells, 100-1000 Okazaki fragments are produced for each replicative DNA polymerase present in the cell. For fast-growing cells, this necessitates rapid recycling of DNA polymerase on the lagging strand. Bacteria produce long Okazaki fragments (1-2 kb) and utilize a highly processive DNA polymerase III (pol III), which is held to DNA by a circular sliding clamp. In contrast, Okazaki fragments in eukaryotes are quite short, 100-250 bp, and thus the eukaryotic lagging strand polymerase does not require a high degree of processivity. The lagging strand polymerase in eukaryotes, polymerase [more...]

Date: 2008-10-17 07:00:00


Free DNA Powerpoint Templates | Free Powerpoint Templates

I found some nice DNA powerpoint templatese here but when I look again, well we HAVE TO PAID FOR IT! Because I won't share the paid powerpoint templates, Then I seek for Free DNA powerpoint templates and go to Microsoft download centre… [more...]

Date: 2008-10-18 17:18:44


mrna dna | DNA Sequencing

Translation: DNA to mRNA to Protein | Learn Science at Scitable Genes encode proteins, and the instructions for making proteins are decoded in two steps: first, a messenger RNA (mRNA) molecule is produced through the transcription . Mrna Dna | TutorVista | Missense mRNA molecules are created when template DNA strands or the mRNA strands themselves undergo a It is synthesised from DNA as and when necessary. It carries. Complementary DNA The central dogma of molecular biology outlines that in synthesizing proteins, DNA is transcribed into mRNA, which is translated into protein. Possibly [more...]

Date: 2010-06-30 09:52:38


New Paper Shows Enzyme-Controlled Movement of DNA Polymer Through ...

This paper addresses a key challenge for DNA strand sequencing: fine control of the translocation of the DNA strand through the nanopore, at a rate that is consistent and slow enough to enable accurate identification of individual DNA bases. The Nature Nanotechnology work shows for the first time that the motion of a strand can be controlled using electronic feedback and that an enzyme can move a strand against a field while located on top of the nanopore. "The techniques described in this paper are an advance towards electronic, single molecule DNA sequencing of DNA strands" said [more...]

Date: 2010-09-28 17:46:34


DNA Powerpoint Template

This outstanding template dispalys a DNA strand which makes the presentations on the themes like DNA chain, genetics, DNA test, hereditary, biochemistry, biotechnology, DNA research, bioinformatics, genomics, microbiology [more...]

Date: 2010-01-18 08:40:00


Epigenetics Papers: DNA Methylation Analysis by MethyLight Technology

Authors discussed each step for this procedure: (1) determining the site of interest for methylation analysis; (2) methylation-specific primers and fluorogenic probes design; (3) genomic DNA isolation; (4) bisulfite conversion; (5) real- time methylation analysis; and (6) data processing. For primer design, some complications particular to the bisulfite modification should be taken into account: All unmethylated cytosine residues in the genome are converted this reduction in genomic complexity reduces the annealing specificity of PCR primers and fluorogenic probes, which not only [more...]

Date: 2007-08-19 01:26:00


Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...

Genetic Fingerprinting of Bacillus thuringiensis Isolates by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)(Published in Nepal Journal of Science and Technology 10(2009) 97-103)Gyan Sundar Sahukhall, Upendra Thapa Shrestha1, Binod Lekhak2, Anjana Singh2, Viswanath Prasad Agrawal11Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal. 2Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepale-mail: gyan633413@gmail.com AbstractRandom Amplified Polymorphic DNA (RAPD) is a method of producing a genetic fingerprint of a [more...]

Date: 2010-01-21 14:06:00


How would you use agarose gel electrophoresis of DNA to detect ...

well you would need a template DNA to compare it to…also some digestion enzyme to cleave it at known base pairs. you would introduce the enzyme to both the template DNA and the one you want to compare to it. digest them. run them in the gel and you’ll see bands at different bp markers for each column. Know better? Leave your own answer in the [more...]

Date: 2010-10-12 12:03:47


DNA Powerpoint Template

Medical slides (11) Pathology slides (11) Keith J Kaplan (5) Mayo Clinic (5) Medical education slides (5) Pathology (4) DNA research (3) blood cells (3) blood transfusion (3) microbiology (3) DNA test (2) Image Library (2) biochemistry (2) bioinformatics (2) biotechnology (2) blood cancer (2) blood donation (2) genetics (2) genomics (2) hereditary (2) AIDS (1) Blood and Tissue (1) Blood templates (1) Blood test (1) Cirrhosis (1) Clinical Pathology (1) Colorectal Cancer slides (1) Coronary artery disease (1) Forensic Pathology (1) HIV (1) Health presentation (1) Heart attack (1) Heart [more...]

Date: 2010-01-18 08:41:00


DNA Transcription (Advanced)

Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT The Central Dogma of Molecular Biology: “DNA makes RNA makes protein” Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place… inserting it with [more...]

Date: 2010-10-03 03:02:09


Identification of Critical Residues for the Tight Binding of Both ...

DNA polymerase λ (Pol λ) is a novel X-family DNA polymerase that shares a 34% sequence identity with DNA polymerase β. Pre-steady-state kinetic studies have shown that the Pol λ-DNA complex binds both correct and incorrect nucleotides 130-fold tighter, on average, than the DNA polymerase β-DNA complex, although the base substitution fidelity of both polymerases is 10(-)(4) to 10(-5). To better understand Pol λ’s tight nucleotide binding affinity, we created single-substitution and double-substitution mutants of Pol λ to disrupt the interactions between active-site [more...]

Date: 2010-09-26 12:46:33


EpiCentral: Examining blocking lesions in ancient DNA

Blocking lesions prevent amplification and sequencing of affected molecules, thus limiting the analysis of DNA derived from ancient samples. Heyn et al. recently developed a new method--polymerase extension profiling (PEP)--that reveals occurrences of polymerase stalling on DNA templates. This sequencing-based technology allows detection of damage on a single-molecule level. The technique used CircLigase™ ssDNA Ligase for high-efficiency ligation of single-stranded adaptors (containing the Roche 454 A sequence) to the 3’ ends of primer-extension products. The authors found evidence of [more...]

Date: 2010-10-07 20:29:00


DNA 04: Free PowerPoint Template of the Day

The Presentation: Conversation with Andrew Abela Andrew Abela is an Associate Professor of Marketing and Chairman of the Department of Business & Economics at the Catholic University of America in Washington, DC. His consulting clients include Microsoft, ExxonMobil, Motorola, Burger King, eBay, and Kimberly-Clark. Prior to academia, he ran the Marketing Leadership Council, was a consultant with McKinsey & Co., and a brand manager at Procter & Gamble. In this Indezine exclusive interview, Andrew discusses his new book, The Presentation: A Story About Communicating Successfully With [more...]

Date: 2006-11-17 07:44:17


Kathara Connection: Kathara Team "DNA Template Activering ...

Kathara Team "DNA Template Activering" workshop in Bloemendaal, Netherlands September 19, 2010 Het Kathara Team nodigt je van harte uit om deel te nemen aan de workshop: DNA Template Activering De sleutel tot spirituele actualisatie Bloemendaal, NL Voortaal Nederlands 19 September 2010 Deze workshop is zeer geschikt voor nieuwkomers. Bovendien geeft deelname een verdieping aan huidige studenten in Keylontische Wetenschap. Aanmelden en informatie: [more...]

Date: 2010-07-10 10:36:23


Ch. 14 Vocabulary: DNA: The Genetic Material

DNA Polymerase Epsilon: (pol ε) Part of the complex of the main replication polymerase used in eukaryotic organisms. DNA Polymerase Delta: (pol δ) The other part of the complex of the main replication polymerase used in eukaryotic organisms. Telomere: A specialized nontranscriptional structure that caps each end of a chromosome. Telomerase: An enzyme that synthesizes telomeres on eukaryotic chromosomes using an internal RNA template. Mutagen: An agent that induces changes in DNA (mutations); incluyes physical agents that damage DNA and chemicals that alter DNA bases. Thymine Dimer: Formed [more...]

Date: 2010-03-10 06:05:00


genomic dna

File type:PDF – Download PDF Reader Higher yields from genomic templates with REDTaq Genomic DNA Genomic or large construct DNA containing target sequence. is digested with a restriction . Genomic DNA Purification Instructor's Manual [more...]

Date: 2010-06-23 09:16:14


Strand Displacement by DNA Polymerase III Occurs through a τ-ψ-χ ...

In addition to the well characterized processive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA templates, the enzyme possesses an intrinsic strand displacement activity on flapped templates. [more...]

Date: 2009-11-06 14:36:37


Polymers

This template with DNA double helix and molecular model will fit presentations on Polymers,powerpoint template on Polymers,template ppt on Polymers,powerpoint slide on Polymers,genetics,research, human genome, genetically engineered , recombinant, vaccine, drag, DNA, [more...]

Date: 2010-09-03 04:37:00


Craft Template - Knit DNA Pattern Help?

One Response to “Knit DNA Pattern Help?” By 2muchstash on Oct 2, 2010 | Reply. Interesting pattern! Since the black tubes are knitted in the round, just clip your yarn, leaving a tail. Using a yarn needle, thread the tail through the [more...]

Date: 2010-10-02 22:32:21


DNA replication

The leading strand is the template strand of the DNA double helix so that the replication fork moves along it in the 3' to 5' direction. This allows the new strand synthesized complementary to it to be synthesized 5' to 3' in the same [more...]

Date: 2010-06-28 03:08:46


Biology Animations: animation of DNA replication

The chain elongation reaction catalyzed by DNA polymerases is a nucleophilic attack by the 3’-OH group of the primer on the innermost phosphorus atom of the deoxynucleoside triphosphate. A phosphodiester bridge forms with the concomitant release of pyrophosphate. The subsequent release of pyrophosphate by pyrophosphatase helps drive the polymerization forward. ***Elongation of DNA chain proceeds 5’-to-3’ direction. ***Template directed enzyme also have nuclease activity. Maurice Wilkins-Rosalind Franklin (x-ray diffraction photos of fibers of DNA) Erwin Chargaff (nucleotide ratios in [more...]

Date: 2007-10-12 15:35:00


Protostadienol synthase from Aspergillus fumigatus: Functional ...

AGCTATCCAAATTAC-30) with 1 lL of plasmid DNA containing the full length cDNA encoding A. fumigatus OSPC as the template. Phusion DNA polymerase (Finnzymes) was used with dNTP. (0.2 mM) in a final volume of 50 lL, according to the manufac- ... MonoFas DNA purification kit (GL Sciences). The second PCR was performed with 10 lL of this fragment as an anti-sense primer, and 1 lL (20 pmol) of the N-terminal primer (AfuOSPC-N-Spe, 50-. CTTGCCTACTAGTATGGCGACAGACAGCAGCATG- 30) [more...]

Date: 2010-01-06 08:00:00


Error-Prone Translesion DNA Synthesis by Escherichia coli DNA ...

When 5-fodU is produced in a template DNA, base substitutions are induced at the site of the lesion . We previously showed that 5-fodU is removed from DNA in E. coli by three DNA glycosylases, MutM, endonuclease III (Nth), [more...]

Date: 2010-08-07 07:00:00


Kinetics of Methylation by EcoP1I DNA Methyltransferase

EcoP1I DNA MTase catalyzes the transfer of methyl groups using a distributive mode of methylation on DNA containing more than one recognition site. A chemical modification of EcoP1I DNA MTase using N-ethylmaleimide resulted in an ... The 1948 bp long M.EcoP1I gene was amplified by a polymerase chain reaction (PCR) using pVK1 construct containing a 2 kb long EcoP1I mod gene as a template with Phusion DNA polymerase, using the forward primer and reverse primer (Table 1) [more...]

Date: 2010-08-23 20:33:16


I Need Attractive Powerpoint Templates On Dna?

Related Posts How Can I Open A Powerpoint From A Different Computer?( 3 ) Iam A Parts Planner/scheduler For Service Engineers (copier/health Science/business Imaging/hvac) Need To Upgr?( 1 ) Is There A Way To Print Speaker Notes In An [more...]

Date: 2010-07-16 10:12:02


Where Can I Find A Powerpoint Presentation About Sailing>?

0 ); Where Were All The Tea Baggers When Bush Was In Office Spending Trillions?( 8 ); How Do You Find A Computers Name To Use In The Cmd Prompt Remote Shutdown?( 2 ); Anybody Tell Me Where I Get Dna Powerpoint Template?( 2 [more...]

Date: 2010-08-02 00:12:06


Nonhomologous DNA End Joining in Cell-Free Extracts

The authors proposed three independent mechanisms for the end-joining based on the sequence at the junctions, which were single-stranded DNA ligation, template-directed ligation, and postrepair ligation . Later, other studies helped [more...]

Date: 2010-08-07 07:00:00


Rapid, nondenaturing RNA purification using weak anion-exchange ...

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. [more...]

Date: 2010-01-25 15:06:07


Investigations of the Inner Workings of T4 Polymerase: the Work of ...

Investigations of the Inner Workings of T4 Polymerase: the Work of Stephen J. Benkovic Abstract Template-Primer-dependent Turnover of (Sp)-dATPαS by T4 DNA Polymerase. The Stereochemistry of the Associated 3′ → 5′-Exonuclease (Gupta, A., DeBrosse, C., and Benkovic, S. J. (1982) J. Biol. Chem. 257, 7689–7692) Spatial Relationship between Polymerase and Exonuclease Active Sites of Phage T4 DNA Polymerase Enzyme (Gopalakrishnan, V., and Benkovic, S. J. (1994) J. Biol. Chem. 269, [more...]

Date: 2009-10-09 13:36:18


Protein Synthesis, DNA Replication - DNA Station

Protein Synthesis, DNA Replication DNA replication is the process of copying a double-stranded DNA molecule to form two double-stranded molecules. The process of DNA replication is a fundamental process used by all living organisms as it is the basis for biological inheritance. As each DNA strand holds the same genetic information, both strands can serve as templates for the reproduction of the opposite strand. The template strand is preserved in its entirety and the new strand is assembled from nucleotides — this process is called “semiconservative replication”. The resulting [more...]

Date: 2010-07-27 02:56:58


.ada.aimi.kisah: dna replication.ape tu?

in this chapter, there's three processes to synthesis protein from DNA. replication(DNA>DNA)> Transcription(DNA>RNA) > Translation(RNA>Protein) just focus on Eukaryotes jea tau. prokaryotes tak masuk dalam syllabus. :) replication of DNA - separation of double strands of DNA is by Helicase. and to prevent the rewinding of the strands, there is SSBp.(Single strand Binding protein) to help. - Leading strand (5' to 3') is copied by DNA Polymerase III which functioned as template for the replication. - lagging strand ( 5' to 3') is produced by DNA Polymerase III and form Okazaki Fragment. [more...]

Date: 2010-08-18 19:04:00


Do you want to live forever? Scientist may have figured out how…

The ends of the chromosomes are capped by telomeres, long thought to have a protective function. Without them, the chromosomes would be shortened during each cell division, because DNA polymerase is unable to copy to the very end of one of the two DNA strands it is replicating.       In the early 1980s, after their fortuitous meeting at a Gordon Research Conference in 1980, Blackburn and Szostak discovered that telo­meres include a specific DNA sequence. Fired up by the novelty of each other's work, they devised experiments that seemed crazy at the time, even to themselves. Szostak took [more...]

Date: 2010-10-05 10:21:00


Evolution News & Views: When "Junk DNA" Isn't Junk: Farewell to a ...

In the Darwinist repertoire, a standard response to evidence of design in the genome is to point to the existence of "junk DNA." What is it doing there, if purposeful design really is detectable in the history of life's development? Of course this assumes that the "junk" really is junk. That assumption has been cast increasingly into doubt. New research just out in the journal Nature Genetics finds evidence that genetic elements previously thought of as rubbish are anything but that. The research describes tiny strands of RNA, previously thought to be junk, that now turn out to play a role in [more...]

Date: 2009-04-28 16:20:29


Pre-Natal Scans And Check ups From London Ultrasound Centre ...

Further scans comprise the Fetal Welfare Scan and also the 20-Week Anomaly Scan, as well as Viability and Dating along with early Pregnancy scans to determine the delivery date Experts in CVS (Chorion Villus Sampling) can test for family genetic problems. The clinic are also specialists in screening for Ovarian Cancer and undertaking pre-natal genetic tests. The clinic in addition undertakes Cocoon 4D Baby Scans, amongst many others. The centre is renowned for its efficiently in performing parenthood testing, fundamentally to determine parentage of an unborn infant LUC is also a centre for [more...]

Date: 2010-10-12 12:10:13


Free molecular biology software for Macs | Bioinformatics Blog

Posted on the October 12th, 2010 under Free mac software by bioinformatics-blog 1. Serial Cloner Serial Cloner is fantastic all-in-one workbench; import and manipulate sequences, construct plasmid and restriction site maps, determine %GC and fragment TM, extract and ligate fragments, perform virtual PCR… and lots more, all in one window using a very intuitive graphical interface. 2. 4Peaks 4Peaks is an extremely user friendly DNA sequence chromatogram viewer and editor from the extremely talented guys at Mekentosj. It’s miles better than any of it’s clunky counterparts… try it, [more...]

Date: 2010-10-12 11:08:47


How the Most Influential Scientists Alive Today Unraveled the ...

DNA has been extensively researched by scientists wishing to further understand the complex way in which life is biochemically formed. DNA contains the genetic information that composes nearly all living organisms. In the early 1950s, molecular biologist James D. Watson led his team of researchers to the ground breaking discovery that DNA is a double-stranded chain, also referred to as a double helix. Using models, he showed how matching nucleotide base pairs from the two DNA strands interlocked to form a double helix. He then showed how the strands separate during replication, and how each [more...]

Date: 2010-09-23 10:31:56


DIY Wedding Invitations Do-It-Yourself Weddings Wedding DNA ...

DIY Wedding Invitations: Wedding DNA Posted by Lisa on Oct 15, 2009. Filed under Budget Wedding Ideas, DIY Wedding Ideas, Invitations, Stuff We Love, Trend Watch, Wedding How-to, Wedding Ideas Comments (4), Permalink In this tough economy, what’s made me smile is how many families have worked together to design their own wedding details: floral arrangements, wedding favors, invitations. Not only do they save money by doing the work themselves, but they also come away with a closer family as they spend a few evenings together baking cookies for personalized wedding favors or [more...]

Date: 2009-10-15 13:14:34


Association between the Herpes Simplex Virus-1 DNA Polymerase and ...

Moreover, UL30, in conjunction with the viral uracil DNA glycosylase (UL2), cellular apurinic/apyrimidinic endonuclease, and DNA ligase IIIα-XRCC1, performs uracil-initiated base excision repair. Base excision repair is required to maintain genome stability as a means to counter the accumulation of unusual bases and to protect from the loss of DNA bases. Here we show that the HSV-1 UL2 associates with the viral replisome. We identified UL2 as a protein that co-purifies with the DNA polymerase through numerous chromatographic steps, an interaction that was verified by [more...]

Date: 2010-08-27 13:40:47


Dynamic DNA Helicase-DNA Polymerase Interactions Assure Processive ...

A single copy of bacteriophage T7 DNA polymerase and DNA helicase advance the replication fork with a processivity greater than 17,000 nucleotides. Nonetheless, the polymerase transiently dissociates from the DNA without leaving the replisome. Ensemble and single-molecule techniques demonstrate that this dynamic processivity is made possible by two modes of DNA polymerase-helicase interaction. During DNA synthesis the polymerase and the helicase interact at a high-affinity site. In this polymerizing mode, the polymerase dissociates from the DNA approximately every 5000 bases. The polymerase, [more...]

Date: 2007-08-31 07:00:00


Blue DNA - Free CSS Templates | TemplateBasics

Categories ▼Web Templates Business Art Fashion Food Technology Sports Medical Entertainment Travel Animals Real Estate Nature Dating Industrial Professionals Personal Holiday Premium ▶Wordpress Themes ▶Joomla Art Fashion Food Technology Sports Medical Entertainment Travel Animals Real Estate Nature Dating Industrial Professionals Personal Holiday Premium '; collapsItems = ''; collapsItems = ''; collapsItems = ''; collapsItems = ''; collapsItems = [more...]

Date: 2009-04-07 23:41:48


Gang of Fort Point - Live Reviews - Boston Phoenix

Four acts sharing no real sonic DNA were on the bill, all apparently playing for the friends who'd come to see them. Viva Viva, Endless Wave, In like Lions, and Banditas were held together — and booked together — by the record label that ... Initial signing Endless Wave boast a textured, neo-shoegaze template dubbed "skygaze." In like Lions are a radio-ready alt-rock quartet reflecting Crow(e)s both Black and Counting. The showcase's headiners, gritty garage boozers Viva [more...]

Date: 2010-10-12 20:39:57


The Randomly Grad Life: My Research Explained: Polymerase Chain ...

New primers attach to your melted DNA. One primer binds to old template DNA and another primers binds to the newly extended DNA. The polymerase once again extends on each strand. On the template strand, the same thing as above happens. But on the new strand, the polymerase will stop because the DNA doesn't go forever. It ends exactly at the end of the first primer. Then the temp raises to 95C and we are ready for the next cycle. Now we almost have exactly what we need. After a few cycles the amount of DNA with ends is in a much larger excess than template DNA and just long copies and that [more...]

Date: 2010-04-21 16:00:00


DNA Art Blog » Blog Archive » PCR Process

We use primer sets that are optimally designed to span the specific genes you choose to have isolated for your canvas. Our technique focuses on the prevention of pre-PCR mis-priming and primer dimerization. Our primers are designed using software with algorithms that check for the potential of secondary structure formation and annealing of primers to itself or within primer pairs. We use hot start Taq Polymerase is designed to enhance the specificity, sensitivity, and yield of the target DNA sequence. Following PCR amplification and purification of the template DNA, we use restriction [more...]

Date: 2010-10-12 15:19:03


DNA repair causes damage - The Scientist - Magazine of the Life ...

A supposedly accurate DNA repair mechanism employed by cells to fix double-strand breaks can surprisingly increase the nearby mutation rate by up to 1400 times, providing a possible explanation for the accumulation of tumor-causing mutations in cancerous tissues. Previously, "we saw that DNA breaks are present in precancerous legions," said molecular oncologist Thanos Halazonetis of the University of Geneva, who did not participate in the research. "These breaks, based on this finding now, could perhaps explain mutations in oncogenes that drive cancer progression." Cancer [more...]

Date: 2007-08-27 07:00:00


dna synthesis

Complete DNA Sequencing & Synthesis Services,DNA Sequencing Analysis System. Custom DNA Synthesis Quick Turnaround w/ 100% Accuracy Guaranteed Expression available. AltaVista found 18,300,000 results DNA replication DNA polymerases, isolated from cells, and artificial DNA primers are used to initiate DNA synthesis at known sequences in a template molecule. … How is dna synthesis made DNA polymerase is the enzyme that catalyzes the reactions involved in … After DNA synthesis is complete, the DNA is typically isolated and purified. … DNA and Protein Synthesis DNA and Protein [more...]

Date: 2010-06-13 03:43:08


genomic DNA PCR protocols and methods

Research news, bioproducts, protocols, reagents, forums genomic DNA PCR > Can someone forward me a general protocol for doing PCR using genomic > DNA as the template? I seem to recall needing about 100 ng of DNA, but > I forget stuff like how much Taq to use, concentration of dNTP's, and > concentration of primers. General protocol for PCR Each person uses a slightly different ratio of reagents. For DNA amplicons up to 500 bp, use 25 uL reaction volumes as follows: On ice add to thin-walled pcr tubes 0.125 uL Taq polymerase (1.25 U, Gibco) 2.5 uL of 10 x PCR buffer minus Mg [more...]

Date: 2004-12-27 00:00:00


Replication of individual DNA molecules under electronic control ...

Nanopores can be used to analyse DNA by monitoring ion currents as individual strands are captured and driven through the pore in single file by an applied voltage. Here, we show that serial replication of individual DNA templates can be achieved by DNA polymerases held at the α-haemolysin nanopore orifice. Replication is blocked in the bulk phase, and is initiated only after the DNA is captured by the nanopore. We used this method, in concert with active voltage control, to observe DNA replication catalysed by bacteriophage T7 DNA polymerase (T7DNAP) and by the Klenow fragment of DNA [more...]

Date: 2010-10-08 10:14:00



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