Our blogs and videos choice: dna synthesis

dna sequencing

DNA sequencing reactions all use a primer to initiate DNA synthesis.  A Quicktime animation of how dideoxy nucleotides are incorporated into DNA in sequencing reactions. Nucleic acid sequence This sequence is written as a succession of letters representing a real or hypothetical DNA molecule or Sequences can be read from the biological raw material through DNA . DNA Sequencing – Real Time DNA analysis Information With the latest chellenges in DNA Sequencing, our organization looks to unravel the myths, truths and most essential assets in the deconstruction of genes. DNA Sequencing The [more...]

Date: 2010-07-09 03:20:29

Blog posts (121) | Videos (29)
 


WHAT HAPPENS WHEN 2 REPLICATION BUBBLES MEET?

So what will happen to the leading and lagging strand? Please post anything that you know here. Anything at all. Thank you. It’s greatly appreciated. Related posts:DNA Replication Cartoon Our second cartoon made for our AP Biology class.... How can DNA synthesis proceed in both directions from a replication origin? Even though DNA polymerase can synthesis DNA in only one... How to define leading strand in DNA replication? I’ve searched online about the leading strand and found a... Why does the lagging strand require more primers than the leading strand in DNA replication? Why does the [more...]

Date: 2010-05-25 17:15:18


DNA strides into organic synthesis

The walker and three 'stations', made from specific DNA sequences, organise themselves along the track using the normal pairing of DNA bases. 'The walker will move unidirectionally along the track,' Liu says. 'We attached chemical reactants to the walker and each station on the track. As the DNA walker moves from station to station, a programmed series of chemical reactions takes place.' The walking strand of DNA moves to each station (blue, red and green) in turn and reacts with the attached reagent © Nature Nanotechnol. In this way, the Harvard team was able to perform three acylation [more...]

Date: 2010-10-10 22:46:00


Welcome to Amino Acids: Amino Acid Translation

The process of copying genetic information from DNA into RNA is called____ protein synthesis replication transcription duplication translation DNA synthesis ___is unique to DNA. adenine cytocine uracil thymine guanine A mRNA sequence with 30 nucleotides codes for______ 15 amino acids 90 amino acids 10 amino acids 3 amino acids Translation is a process which converts the information in ____into ___ "DNA, mRNA" "mRNA, DNA" "protein, DNA" "mRNA, protein Get the answers... Missense mutation in which part of KRT5 gene does epidermolysis bullosa with mottled pigmentation form? (Answers: 1) [more...]

Date: 2008-01-19 08:00:00


Best 2010 Ebook Collection Part 4

Dean J.R. - - - Extraction Methods for Environmental Analysis Dean M. - - - The Human ATP-Binding Cassette (ABC) Transporter Superfamily Dean M.W. - - - $30 Music School Dean T. - Gleek J. - - Internet Marketing Secrets Revealed Deans G.K. - Kroeger F. - Zeisel S. - Winning the Merger Endgame: A Playbook for Profiting from Industry Consolidation Deans P.C. - - - E-commerce and M-commerce technologies Deb S. - - - Video data management and information retrieval Deb S. (ed.) - Deb S.P. (ed.) - - p53 Protocols Debarre O. - - - Higher-Dimensional Algebraic Geometry Debevec P. - - - [more...]

Date: 2010-10-11 18:41:15


DNA-PROKIDS program to help eliminate international human trafficking

Scientists at the University of North Texas Health Science Center (UNTHSC) have recently received a $500,000 grant from The Life Technologies Foundation for use in development of the DNA-PROKIDS Project (Program for Kids Identification with DNA Systems). This program will be designed to deter international human trafficking through the use of DNA testing, which will help to reunite abducted and homeless children with their biological parents/families. Through PROKIDS, children who are located and found to be associated with any human trafficking activity, i.e. illegal adoptions, living on the [more...]

Date: 2010-10-02 00:39:43


Chronic Lymphocytic Leukemia (CLL) as a Platform for Functional ...

We chose cytarabine (Ara-C), a drug not considered effective for CLL (e.g. low proliferative rate, no likelihood of DNA synthesis inhibition, no likelihood of cytotoxicity). To our delight, low doses or Ara-C proved highly effective in controlling even the most advanced cases of CLL as we then reported. CLL became one of our favored models for the study of human tumor biology, enabling us to study drug responses at the molecular level. Many of the observations that we made in this hematologic malignancy granted us insights that we continue to apply in solid tumors today. search About... Dr. [more...]

Date: 2010-10-13 16:39:38


Chrysotile Properties and the Mesothelioma Plague | Mesothelioma Facts

Significant increases in epithelial cells of the bronchi, the interstitial compartment of the lung, and mesothelial cells incorporating 5-bromo-2′-deoxyuridine, an indication of DNA synthesis, were noted in the higher chrysotile group at 5 days, but labeling in all cell compartments was comparable with that occurring in sham controls at later time points. Indicators of inflammation, increased cell proliferation, and pulmonary fibrosis were not observed in the lungs of rats exposed to the lower concentration of chrysotile. Thus, results indicate that cellular and molecular markers of [more...]

Date: 2010-10-10 23:38:16


Important questions 4 CSIR | BioTecNika.Org

Todays set of questions are: 1)    Name two nonpolar amno acids. Sickle cell anaemia results from a substitution of a valine for a glutamate. What do you expect the effect might be if mutation were to have placed a leucine at that site? Or an aspartate? (Karp, 80) 2) Match the following: Group—A                                     Group—B a) RNA polymerase                  i) NADPH formation b) Lipids                                   ii) DNA polymerase-I c) [more...]

Date: 2010-09-26 02:05:57


Sounds Seen # 1 – 1 opponent | DNA Sequencing

Complete DNA Sequencing & Synthesis Services,DNA Sequencing Analysis System. Sounds Seen # 1 – Contestant 1 hybrid Rob. Sounds winner has been a competition organized by VJ Instruments Livid and The Alamo Drafthouse in Austin, Texas. VJs, DJs, video artists and performers, scratch, mix and mash audio and video in real-time multimedia experience. This video has seen artists Sounds # 1 of the original Alamo Drafthouse on September 24, 2006 in Austin, Texas. Possibly Related Posts: 1994 INAC Seaweed collection expedition to the Antarctic Peninsula Epigenetics Webinar (Part 2 of 4) As a [more...]

Date: 2010-10-11 19:51:16


Ultrasensitive DNA detection

Rapid and sensitive DNA detection methods have a wide range of uses from cancer and disease diagnosis to the detection of harmful bacteria in food and drink. Previous methods rely on optical methods and a time consuming pre-amplification of the sample, which while sensitive often take several weeks and require bulky apparatus. Electrochemical methods have been used to improve the speed and portability. Now, Elena Ferapontova, Kurt Gothelf and colleagues at Aarhus University have developed a lipase-based electrochemical technique that can detect as little as 20 attomoles (2 x 10-17 moles) of [more...]

Date: 2010-03-12 23:37:21


Active Site Mutations in Mammalian DNA Polymerase {delta} [Enzymology]

Active Site Mutations in Mammalian DNA Polymerase δ Alter Accuracy and Replication Fork Progression* Michael W. Schmitt‡, Ranga N. Venkatesan§, Marie-Jeanne Pillaire¶, Jean-Sébastien Hoffmann¶, Julia M. Sidorova§ and Lawrence A. Loeb‡§,1 From the Departments of ‡Biochemistry and §Pathology, University of Washington School of Medicine, Seattle, Washington 98195 and the ¶Genetic Instability and Cancer Group, Institute of Pharmacology and Structural Biology, CNRS and University of Toulouse, 31077 Toulouse, France 1 To whom correspondence should be addressed: University of [more...]

Date: 2010-10-08 13:45:11


Vitamin B12 – Also known as cobalamin | Vitamin D Deficiency Symptoms

It is necessary during cell division for rapid DNA synthesis; especially in tissues where cell division occurs at a fast rate specifically bone marrow for the synthesis of red blood cells. Deficiency of B12 leads to production of Defective DNA bearing cells called megaloblast. This condition is called anemia. Symptoms are excessive tiredness, breathlessness, restlessness, pallor, and low immunity, sore tongue and menstrual disorder. B12 plays a major role in well being of nervous system. Nerves are surrounded by an insulating fatty sheath comprised of a complex protein called myelin. B12 is [more...]

Date: 2010-10-10 09:00:23


Marine Drugs | Free Full-Text | Antiviral Lead Compounds from ...

It inhibits viral DNA polymerase and DNA synthesis of herpes, vaccinica and varicella zoster viruses. However due to the discovery of new types of viruses and emergence of drug resistant strains, it is necessary to develop new antiviral lead compounds continuously. Several sponge derived antiviral lead compounds which are hopedto be developed as future drugs are discussed in this review. Supply problems are usually the major bottleneck to the development of these compounds as drugs during clinical trials. However advances in the field of metagenomics and high throughput microbial cultivation [more...]

Date: 2010-10-11 07:00:00


DNA sequencing « Home Workout Equipment

Noesis of DNA sequences has become indispensable for basic biological research, other enquiry legs using DNA sequencing, and in numerous applied champaigns such as diagnostic, biotechnology, forensic biology and biological systematics. The advent of DNA sequencing has significantly accelerated DNA Synthesis biological inquiry and discovery. The rapid fastness of sequencing attained with modernistic DNA sequencing technology has been instrumental in the sequencing of the human genome, in the Human Genome Project. Related projects, frequently by scientific collaboration across continents, [more...]

Date: 2010-10-12 16:39:33


Study Says Nutrition And Heredity Are Genetically Linked

About News News From All Over The World, Right To You ... View News's Profile A challenging goal in biology is to understand how the principal cellular functions are integrated so that cells achieve viability and optimal fitness under a wide range of nutritional conditions. Scientists from the French research centers INRA and CNRS showed by genetic approaches that, in the model bacterium Bacillus subtilis, central carbon metabolism (which generates energy from nutrients) and replication (which synthesizes DNA), two key functions in the fields of nutrition and heredity, are tightly linked. [more...]

Date: 2007-05-16 07:00:00


protein synthesis diagram | DNA Sequencing

Protein Synthesis Diagram: simplified picture of a cell with picture of protein synthesis showing messenger RNA, transfer RNA and ribosomes, by Russell Kightley Media. Protein Synthesis During protein synthesis, which type of molecule attaches particular amino acid molecules The diagram represents molecules involved in protein synthesis. Protein Synthesis Essential details of protein synthesis are covered in many courses,The diagram at right is from the file to be explored by Chime below. For images depicting the proposed. Possibly Related Posts: 1994 INAC Seaweed collection expedition to [more...]

Date: 2010-07-28 03:40:05


Break Out the Brazil Nuts -- You Need More Selenium - thatsfit.ca

Along with other antioxidant nutrients, selenium helps to block reactions that lead to free radical formation; free radicals which can cause DNA mutation leading to cancer. Glutathione peroxidase is one of the body's most powerful antioxidant enzymes, used by the liver to detoxify a wide range of potentially toxic molecules. Selenium also protects against cancer by inducing DNA repair and synthesis in damaged cells, repairing free radical damage that has already occurred. It inhibits the spread of cancer cells and induces tumor cells' natural self-destruct sequence. Plus, selenium is [more...]

Date: 2010-09-27 18:00:00


How Can Diet Affect Sperm Quality?

Zinc and folic acid, play a role in the synthesis of DNA and RNA. Deficiency of zinc is as a clinical symptom of reduced spermatogenesis and reduced fertility. Zinc is found in shellfish and lean meat, and folic acid in dark green leafy vegetables and fortified cereals What dietary factors reduce sperm quality? Along with diet, some other factors in the lifestyle of the man may be responsible for infertility. Obesity: The increased body fat binds free testosterone in the blood, and probably favors the conversion of testosterone to female hormone estrogen. Inspect and maintain your weight in a [more...]

Date: 2010-10-11 04:51:49


SURE Animations for Higher Biology | Citocromo P450

Posted on septiembre 14, 2010, 8:12 am, by admin, under Citocromo P450. This is a showreel for SURE Animations, a high quality 3-D animation suite for the SQA Higher Biology curriculum. The software contains 20 fully annotated animations with additional detailed descriptions of the physical processes. The animations cover: Cell types, Cell absorption, Diffusion and osmosis, Plasma membrane fluid mosaic model, Active transport, Glycolosis, Mitochondrial structure, Krebs’ cycle, Cytochrome system, Photosynthesis: Light-dependent stage, Photosynthesis: Calvin cycle, Antibody, DNA, DNA and RNA, [more...]

Date: 2010-09-14 08:12:34


Penicilliosis | Treatment for

Flucytocine (5-FC) is a synthetic fluorinated pyrimidine that inhibits fungal protein and DNA synthesis. There are limited data on the use of 5-FC in the treatment of penicilliosis. The average dose is 50-150 mg/kg given as four divided doses. The most common side effects are diarrhea, nausea and vomiting. Abnormal elevations of liver function tests developed in about 5% of the patients. Thrombocytopenia and leukopenia may occur if excessive serum concentrations are maintained. The effect is reversible if treatment is discontinued. The drug is best avoided in AIDS patient due to several [more...]

Date: 2010-10-12 07:25:27


DNASwab by DNAExam Combines Convenience with Confidentiality at Home

Once the samples arrive at the DNA Identity Testing Center, they are analyzed twice over using 16 STR loci DNA technology and then confirmed by a minimum of three DNAExam technicians. DNASwab kits can be ordered online through 800DNAExam.com. All testing is performed on-site at the DNA Identity Testing Center and never outsourced to any third party. Results are available in three to seven business days. Expedited services are available. In addition to tests that determine family lineage for private purposes, DNAExam also offers DNA analyses for cases of custody, child support, immigration, [more...]

Date: 2010-09-09 02:28:31


Ch. 14 Vocabulary: DNA: The Genetic Material

DNA Polymerase Epsilon: (pol ε) Part of the complex of the main replication polymerase used in eukaryotic organisms. DNA Polymerase Delta: (pol δ) The other part of the complex of the main replication polymerase used in eukaryotic organisms. Telomere: A specialized nontranscriptional structure that caps each end of a chromosome. Telomerase: An enzyme that synthesizes telomeres on eukaryotic chromosomes using an internal RNA template. Mutagen: An agent that induces changes in DNA (mutations); incluyes physical agents that damage DNA and chemicals that alter DNA bases. Thymine Dimer: Formed [more...]

Date: 2010-03-10 06:05:00


DNA parentage testing when the alleged parent is deceased – DNA Tests

These types of samples will require mtDNA testing techniques, as opposed to standard STR DNA, but can be useful in determining parentage. Since, often times, success can not be guaranteed with samples of the deceased, it is recommended that one consult with a DNA testing laboratory or expert to be advised of the best option for your specific situation. In some cases, it may be helpful to rely on samples from the alleged parent’s family members, which can sometimes indirectly establish parentage. About DNAExam The DNA Identity Testing Center of Bio-Synthesis Inc., headquartered in [more...]

Date: 2010-08-12 17:19:40


Healing heart attack victims, one cell at a time

Buchholz found that people born around or after the nuclear bomb tests corresponded to atmospheric concentrations several years after the subjects' birth, indicating substantial postnatal DNA syntheses. “By analyzing individuals born at different times before 1955, it is possible to establish the age up to which DNA synthesis occurs, or whether it continues beyond that age,” Buchholz said. In the study, carbon 14 concentrations were elevated in subjects compared to those people born up to 22 years before the beginning of nuclear bomb tests. “DNA of myocardial cells is synthesized [more...]

Date: 2009-04-02 18:00:00


Reconstructing human appearance from DNA -- Signs of the Times News

This is a relatively robust, but not foolproof, gender test, according to Kayser. Hair colour prediction underway Beyond gender, the human traits with highest predictive accuracy are those affected by pigmentation, simply because they are the least complex. Red hair colour, which is determined by a single gene, can be predicted with a certainty of around 93%. For other hair types, accuracy is slightly lower, but still promising. Kayser reported confidence levels as high as 85, 81 and 80% for predicting black, brown and blond hair respectively. Perhaps the most progress has been made in [more...]

Date: 2010-09-28 13:51:40


Dynamic DNA Helicase-DNA Polymerase Interactions Assure Processive ...

A single copy of bacteriophage T7 DNA polymerase and DNA helicase advance the replication fork with a processivity greater than 17,000 nucleotides. Nonetheless, the polymerase transiently dissociates from the DNA without leaving the replisome. Ensemble and single-molecule techniques demonstrate that this dynamic processivity is made possible by two modes of DNA polymerase-helicase interaction. During DNA synthesis the polymerase and the helicase interact at a high-affinity site. In this polymerizing mode, the polymerase dissociates from the DNA approximately every 5000 bases. The polymerase, [more...]

Date: 2007-08-31 07:00:00


Quick Nucleic Acid Facts

Anne Marie's Chemistry BlogBy Anne Marie Helmenstine, Ph.D., Chemistry Guide | My Bio | RSSMy BioMy BlogMy ForumRSS If you're taking general chemistry, organic, or biochemistry, you'll need to understand some basic concepts about nucleic acids, the polymers used to code the genetic information of organisms. Here are some quick nucleic acid facts to get you started: Nucleic acids are the molecules that code the genetic information of organisms. The two nucleic acids used in repair, reproduction and protein synthesis are deoxyribonucleic acid (DNA, shown) and ribonucleic acid [more...]

Date: 2010-09-27 17:16:46


BIVDA.COM - OligoMix® Oligonucleotide Mixture

OligoMix.com LC Sciences provides a unique new tool, OligoMix® enabling low cost, multiplex custom oligonucleotides for various applications serving diverse needs in genomics, proteomics, protein engineering, synthetic biology, drug discovery, and emerging new fields of science such as next-generation sequencing. OligoMix® is a MIXTURE of a large number of DESIGNED synthetic DNA oligonucleotide sequences delivered in a single microtube. OligoMix® is a product developed in response to the need for pooled oligonucleotides used in multiplexing reactions. We synthesize thousands of [more...]

Date: 2010-10-10 21:29:50


Strand Transfer and Elongation of HIV-1 Reverse Transcription Is ...

These results indicate that the cell factor(s) may improve reverse transcription by facilitating DNA strand transfer and DNA synthesis. It also had a protective function for the reverse transcription products, but it is unclear if this is related to improved DNA [more...]

Date: 2010-10-08 02:25:16


MTHFR A1298C polymorphism and idiopathic male infertility Singh K ...

The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyses the formation of folate intermediates that are vital for DNA synthesis and methylation reactions. C677T and A1298C variants of MTHFR result in reduced plasma folate and increase the susceptibility to various multifactorial disorders. We have already shown that homozygosity for 677 (C ®T) mutation in the MTHFR gene, is a risk factor for idiopathic male infertility in an Indian population. Aim: Recently, we showed that homozygosity for the 677(C;T) mutation in the MTHFR gene is a risk factor for idiopathic male infertility and [more...]

Date: 2010-10-08 20:51:33


IDT rises to 6th in 'Best Places to Work'

Named in the top 10 small companies for the second consecutive year Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has been named one of the top 10 ‘Best Places to Work in Industry’, in a poll of research staff conducted by a leading magazine. Published in the May issue of ‘The Scientist’, the poll put IDT 6th in the small company category, exceeding its 2009 placement of 8th. Dr. Joseph A. Walder, IDT’s founder and CEO commented, “We are pleased to be named for a second year as a leading place to work, especially since this also reflects the [more...]

Date: 2010-06-09 11:28:36


Productive Nanosystems: The Movies

Productive Nanosystems: The Movies by Eric Drexler on 30 January 2009 In his comment on Molecular Machine Assembly: The Movie, Drew Whitehouse reminded me of a set of excellent animations of biological productive nanosystems, work done by Drew Berry. These videos are based on scientific data describing molecular structure and function, and from what I’ve seen, Drew Berry’s work is the best of its kind. Below are the most complete and directly accessible videos I’ve found. Productive nanosystems are nanoscale machines that make atomically precise products under programmable control, and [more...]

Date: 2009-01-30 01:37:02


Structure and mechanism of human DNA polymerase eta.

Structure and mechanism of human DNA polymerase eta. Nature. 2010 Jun 24;465(7301):1044-8 Authors: Biertümpfel C, Zhao Y, Kondo Y, Ramón-Maiques S, Gregory M, Lee JY, Masutani C, Lehmann AR, Hanaoka F, Yang W The variant form of the human syndrome xeroderma pigmentosum (XPV) is caused by a deficiency in DNA polymerase eta (Poleta), a DNA polymerase that enables replication through ultraviolet-induced pyrimidine dimers. Here we report high-resolution crystal structures of human Poleta at four consecutive steps during DNA synthesis through cis-syn cyclobutane thymine dimers. Poleta acts [more...]

Date: 2010-06-26 11:02:58


Questions to Ask Your DNA Testing Laboratory – DNA Tests

Un-accredited labs do not have to abide by any standards and, therefore, may not offer the best service and most accurate tests. Of the hundreds of laboratories advertising DNA testing services worldwide, only 41 are currently AABB accredited. Do you outsource testing? Many of the companies you will come across through online searches are actually brokers, not laboratories. Since brokers do not perform the testing themselves, they must send your information and samples to a third party laboratory. Some brokers may work with reputable laboratories, but others do not. Working with any broker [more...]

Date: 2010-08-12 17:01:33


EveryDay Science » Blog Archive » New roles for new genes ...

Thanks to new technological advances in genomic sequencing , we know that much of the DNA genome is transcribed into RNA but only a small portion of this RNA (transcriptome ) contains sequences that encode for the synthesis of proteins. Thus, although long RNA fragments are non-coding transcriptome most mammals, their cellular functions are still unknown. Scientists have already described the role of small RNAs in gene regulation but we still know very little about the long fragments . The long noncoding RNAs are RNA fragments longer than 100 nucleotides. The authors of this paper have shown [more...]

Date: 2010-10-01 14:35:23


IDT earns ISO 9001:2008 certification at its European ...

LEUVEN, Belgium: Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, today announced that the production processes for synthetic oligonucleotides at its European headquarters in Leuven, Belgium have achieved ISO 9001:2008 certification. All three IDT oligonucleotide facilities (Coralville, Iowa, San Diego, California, and Leuven, Belgium) now offer ISO 9001:2008 certification, with the Coralville, Iowa manufacturing headquarters also providing ISO 13485:2003 certification for the manufacture of diagnostic oligonucleotides. In addition, IDT’s Leuven facility [more...]

Date: 2010-04-26 15:36:03


Integrated DNA Technologies Introduces Custom Labels - Iowa City ...

Coralville Company First to Introduce Custom Labels Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, is the first company in the industry to introduce Custom Label Design to their website. With the Custom Label Design Tool, customers can tailor the information provided on their labels. By designing different custom labels, users can easily organize and manage their orders to prevent potential confusion in order receipt and retrieval. A drag-and-drop interface allows them to include information from a variety of sources including PO#, Sequence Name, Molecular [more...]

Date: 2009-06-19 18:47:55


dna protein synthesis

DNA is made of four simple building blocks, yet it contains all of the information.the information in a DNA sequence to build a protein, then build one yourself! . Possibly Related Posts: 1994 INAC Seaweed collection expedition to the Antarctic Peninsula Epigenetics Webinar (Part 2 of 4) As a sequence of the genome sequencing of 7 Products Sounds Seen # 1 – 1 opponent Bioluminescent [more...]

Date: 2010-06-13 06:02:45


DNA Sequencing Videos,Photos,Informations,Details,Collections ...

DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA. The most popular method for doing this is called the dideoxy method or Sanger method (named after its inventor, Frederick Sanger, who was awarded the 1980 Nobel prize in chemistry for this achievment). DNA is synthesized from four deoxynucleotide triphosphates. The top formula shows one of them: deoxythymidine triphosphate (dTTP). Each new nucleotide is added to the 3′ -OH group of the last nucleotide added. Link to discussion of DNA synthesis. The dideoxy method gets its name from [more...]

Date: 2010-08-25 06:59:00


Alnylam Scientists Present New Data on RNAi Therapeutics at ...

Further, several of the presentations describe the design, synthesis, and analytical characterization of siRNAs with optimized properties, including: “Solid-phase synthesis of 5'-di- and tri-phosphates and their modified analogs of DNA, RNA and chemically modified oligonucleotides,” by Ivan Zlatev, Ph.D., Scientist; “Parallel high throughput synthesis of chemically modified 21-27mer siRNA sequences,” by Satya Kuchimanchi, Ph.D., Associate Director, Small Scale Synthesis; “Novel method for the confirmation of siRNA sequence by LC-MS/MS,” by Gary Lavine, Ph.D., Senior [more...]

Date: 2010-08-24 12:13:50


Cytoplasmic & Nuclear RNA Purification Kit

RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]

Date: 2010-08-16 04:58:00


Oligonucleotide Synthesis: Methods and Applications (Methods in ...

Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene [more...]

Date: 2004-07-30 07:00:00


dna protein synthesis

Proteins are widely used in cells to serve diverse functions of the steps involved in protein synthesis. Step 1: DNA Transcription Possibly Related Posts: 1994 INAC Seaweed collection expedition to the Antarctic Peninsula Epigenetics Webinar (Part 2 of 4) As a sequence of the genome sequencing of 7 Products Sounds Seen # 1 – 1 opponent Bioluminescent [more...]

Date: 2010-07-11 04:05:47


IDT rises to 6th in 'Best Places to Work'

CORALVILLE, IA: Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has been named one of the top 10 ‘Best Places to Work in Industry’, in a poll of research staff conducted by a leading magazine. Published in the May issue of ‘The Scientist’, the poll put IDT 6th in the small company category, exceeding its 2009 placement of 8th. Dr. Joseph A. Walder, IDT’s founder and CEO commented, “We are pleased to be named for a second year as a leading place to work, especially since this also reflects the quality and expertise of our staff. IDT has always [more...]

Date: 2010-05-26 12:33:01


USMLE step 1 Mcq 118:protein synthesis is the process whereby DNA ...

image. Question: protein synthesis is the process whereby DNA encodes for the production of amino acids and proteins. In this process what is a unit of three nucleotides on the mRNA called? a) ribozyme b) antiribozyme c) base-pair [more...]

Date: 2010-08-06 01:48:37


A quick 5 second animation of the chemistry involved in DNA synthesis

Click here for the short animation: http://bioweb.uwlax.edu/GenWeb/Molecular/Theory/DNA_synthesis/dnasynth.mov And here's the explanation: When DNA is synthesized the free 3´ hydroxyl (OH) group from the growing strand of DNA attacks the a phosphate on the next base to be added (see the red arrows below). Pyrophosphate is released and the new base forms a phosphodiester bond with the growing strand of DNA. The free 3´ hydroxyl group is then free to attack the next base to be added. This reaction is catalyzed by DNA polymerases. Similar reactions are catalyzed by RNA polymerases in the [more...]

Date: 2009-02-19 07:13:00


DNA Synthesis and Repair Genes RRM1 and ERCC1 in Lung Cancer — NEJM

Original Article from The New England Journal of Medicine — DNA Synthesis and Repair Genes RRM1 and ERCC1 in Lung Cancer. ... of gemcitabine and platinum in non-small-cell lung cancer. J Clin Oncol 2006;24:4731-4737 CrossRef | Web of Science | Medline. 18. Sandler AB , Nemunaitis J , Denham C , et al. Phase III trial of gemcitabine plus cisplatin versus cisplatin alone in patients with locally advanced or metastatic non-small-cell lung cancer. J Clin Oncol 2000;18:122- 130 [more...]

Date: 2007-02-22 05:00:00


The Aspergillus Website blog: New Research to Help Identify ...

those that target DNA/RNA synthesis e.g. flucytosine. Mammalian cells make extensive use of DNA & RNA so this drug lacks specificity. Difficult to use. Clearly the best existing target in terms of specificity is the cell wall. In this news report it states that this research group have just announced the $1.5 million funding of new research into drugs that will act on a component of the construction of cell walls (UDP-galactopyranose mutase) in fungi with the aim of disrupting the process and thus making it very difficult for the fungus to make its own cell wall and grow normally. This [more...]

Date: 2010-09-29 10:13:00


When will the average price for commercially available DNA ...

Is the price of DNA synthesis going the way of Moore's Law? Recent prices from top synthesis companies (average price per base pair): GeneArt: $0.53 DNA 2.0: $0.69 Codon Devices: $0.79 Mr. Gene: $0.39. See http://www.1centbp.com/ for [more...]

Date: 2010-09-16 18:40:00



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