Our blogs and videos choice: dna stability

Biosensors: Morpholino offers you more

Molecular biologists and geneticists often use DNA microarrays to detect DNA, measure gene expression levels and detect the single-nucleotide polymorphisms that are responsible for various genetic diseases. A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the [more...]

Date: 2010-10-13 09:52:22

Blog posts (35) | Videos (15)

Biosensors: Morpholino offers you more

A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the ability to withstand wear and tear over many cycles). Fig. 1: Scanning electron microscopy image (left) of a cluster of parallel SiNWs, and a schematic illustration (right) of the Morpholino-based sensing [more...]

Date: 2010-10-13 12:52:44

DUX4 Gene Product May Cause FSHD, A Form of Muscular Dystrophy ...

In those susceptible to this form of muscular dystrophy, this DNA mutation stabilizes the product of the DUX4 gene and thus causes the gene to be more active. “This provides a new and unifying model for FSHD because it will focus future research on determining whether the DUX4 protein causes FSHD, as indicated by our consortium’s genetic analysis,” Tapscott said. Corresponding author Silvere van der Maarel, Ph.D., professor of medical epigenetics in the Department of Human Genetics at Leiden University Medical Center in the Netherlands, led the study in collaboration with Tapscott and [more...]

Date: 2010-08-21 01:01:17

Insights into watermelon genetic diversity

Insights into watermelon genetic diversity by Jeremy on September 29, 2010 There’s no doubt that most of the time when we non-experts think of genetic diversity in DNA terms, we think about alterations in the actual DNA sequence. Change the genetic code and there’s a chance you change the nature of a gene and as a result change the outward appearance, the phenotype, of an organism. There have been lots of studies relating the differences among species to differences in the DNA code of this sort, but far fewer looking at the differences among varieties of a single species. Amnon Levi and [more...]

Date: 2010-09-29 05:55:20

Structure and mechanism of human DNA polymerase eta.

Structure and mechanism of human DNA polymerase eta. Nature. 2010 Jun 24;465(7301):1044-8 Authors: Biertümpfel C, Zhao Y, Kondo Y, Ramón-Maiques S, Gregory M, Lee JY, Masutani C, Lehmann AR, Hanaoka F, Yang W The variant form of the human syndrome xeroderma pigmentosum (XPV) is caused by a deficiency in DNA polymerase eta (Poleta), a DNA polymerase that enables replication through ultraviolet-induced pyrimidine dimers. Here we report high-resolution crystal structures of human Poleta at four consecutive steps during DNA synthesis through cis-syn cyclobutane thymine dimers. Poleta acts [more...]

Date: 2010-06-26 11:02:58

Variation, patterns, and temporal stability of DNA methylation ...

Talens, RP, Boomsma, DI, Tobi, EW, Kremer, D., Jukema, JW, Willemsen, G., Putter, H., Slagboom, PE, Heijmans, BT Variation, patterns, and temporal stability of DNA methylation: considerations for epigenetic [more...]

Date: 2010-08-31 07:02:19

Nanodevices on Track as Medical Tools | Focus

At left is a tensegrity structure made of wooden rods and string and, center, a diagrammatic image of one constructed with DNA struts (colored ladders folded into rods) and DNA cable strands (colored single lines). At right is an electron micrograph of an actual nanoscale tensegrity structure built using the new DNA-based, self-assembling nanofabrication capabilities. The scale bar equals 20 nanometers. “This new self-assembly–based nanofabrication technology could lead to nanoscale medical devices and drug delivery systems, such as virus mimics that introduce drugs directly into diseased [more...]

Date: 2010-07-30 20:45:40

Bile Acid Extends the life of Yeast | Niclear Fusion - Nuclear Fusion

... altering oxidation-reduction processes in mitochondria, enhancing resistance to oxidative and thermal stresses, suppressing mitochondria-controlled apoptosis, and enhancing stability of nuclear and mitochondrial DNA. [more...]

Date: 2010-10-07 16:51:26

The reanimated zombie gene | MetaFilter

This doesn't sound like non-coding DNA stabilizing mRNA, but the number of D4Z4 repeats affecting how often the DUX4 gene gets transcribed. Fewer repeats means more DUX4 transcription. More DUX4 transcription means more DUX4 protein, [more...]

Date: 2010-08-19 20:15:53

DNA Replication Factor C1 Mediates Genomic Stability and ...

e National Center for Plant Gene Research, Beijing 100193, China. 2 Address correspondence to gongzz{at}cau.edu.cn. Genetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA [more...]

Date: 2010-07-16 07:00:00

GeoVax Labs Announces Issuance of 6th Patent US Patent 7795017 ...

Harriet Robinson, Chief Scientific Officer and lead inventor on the patent said, "This patent covers our pGA series of plasmids that are not only useful for delivery of DNA vaccines but have proven to have outstanding stability during [more...]

Date: 2010-10-07 18:12:58

BMI1-mediated histone ubiquitylation promotes DNA double-strand ...

Ismail Hassan Ismail1,2, Christi Andrin1, Darin McDonald1, and Michael J. Hendzel1 1Department of Oncology, University of Alberta, Edmonton, T6G 1Z2 Alberta, Canada 2Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt Correspondence to Michael J. Hendzel: mhendzel{at}ualberta.ca Abstract Polycomb group (PcG) proteins are major determinants of cell identity, stem cell pluripotency, and epigenetic gene silencing during development. The polycomb repressive complex 1, which contains BMI1, RING1, and RING2, functions as an E3-ubuiquitin ligase. We found that BMI1 and [more...]

Date: 2010-10-04 15:56:59

Characterization of a Y-Family DNA Polymerase eta from the ...

Similar experiments were performed in a study on TLS past a benzopyrene adduct by another thermostable Y-family DNA polymerase, archaeal Dpo4, although the organic solvents were added to stabilize the benzopyrene moiety in the [more...]

Date: 2010-06-29 07:00:00

BMI1-mediated histone ubiquitylation promotes DNA double-strand ...

We found that BMI1 and RING2 are recruited to sites of DNA double-strand breaks (DSBs) where they contribute to the ubiquitylation of γ-H2AX. In the absence of BMI1, several proteins dependent on ubiquitin signaling, including 53BP1, BRCA1, and RAP80, are impaired in recruitment to DSBs. Loss of BMI1 sensitizes cells to ionizing radiation to the same extent as loss of RNF8. The simultaneous depletion of both proteins revealed an additive increase in radiation sensitivity. These data uncover an unexpected link between the polycomb and the DNA damage response pathways, and suggest a novel [more...]

Date: 2010-10-06 20:38:28

Association between the Herpes Simplex Virus-1 DNA Polymerase and ...

Moreover, UL30, in conjunction with the viral uracil DNA glycosylase (UL2), cellular apurinic/apyrimidinic endonuclease, and DNA ligase IIIα-XRCC1, performs uracil-initiated base excision repair. Base excision repair is required to maintain genome stability as a means to counter the accumulation of unusual bases and to protect from the loss of DNA bases. Here we show that the HSV-1 UL2 associates with the viral replisome. We identified UL2 as a protein that co-purifies with the DNA polymerase through numerous chromatographic steps, an interaction that was verified by [more...]

Date: 2010-08-27 13:40:47

Suzuki Kizashi Sport GTS and Sport SLS Trim Levels Added for 2011 ...

Furthermore, its active and energetic identity, a core component of the Suzuki brand DNA, is augmented by the vehicle’s overall mission to provide strong yet refined street performance. For 2011, Suzuki adds a Sport trim to the GTS and SLS models, with a host of external and internal modifications that include a more muscular front fascia (with chrome accents) and lower grille; body side sill extensions; lower body side molding with chrome accents; a trunk mounted spoiler; custom lightweight 18-inch alloy wheels appropriate to a flagship vehicle and typical of more expensive sport sedans; a [more...]

Date: 2010-10-13 14:36:00

The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...

There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]

Date: 2010-10-04 21:15:46

Alnylam Scientists Present New Data on RNAi Therapeutics at ...

Further, several of the presentations describe the design, synthesis, and analytical characterization of siRNAs with optimized properties, including: “Solid-phase synthesis of 5'-di- and tri-phosphates and their modified analogs of DNA, RNA and chemically modified oligonucleotides,” by Ivan Zlatev, Ph.D., Scientist; “Parallel high throughput synthesis of chemically modified 21-27mer siRNA sequences,” by Satya Kuchimanchi, Ph.D., Associate Director, Small Scale Synthesis; “Novel method for the confirmation of siRNA sequence by LC-MS/MS,” by Gary Lavine, Ph.D., Senior [more...]

Date: 2010-08-24 12:13:50

Cellular Clock Movement Supports Seismic Modifications In ...

Human cells have only got so many lives, same as cats. When divided over time they are altered, slow down and gradually quit separating – this is referred to in scientific terms as cellular senescence – . Already biologists understand that a cellular timer made up of structures at the end of the chromosome called Telomeres, document any activity regarding the cells’ lives and the number that have been exhausted. Until this point, researchers have yet to learn how the running of the clock determines the mortality of the cells. An investigation [more...]

Date: 2010-10-11 21:49:26

Natural Diet and Lifestyle Reverse the Effects of Aging | Health News

While DNA and genetics play a minor role, poor diet and lifestyle directly contribute to the leading causes of mortality, coronary artery disease, cancer, stroke, and dementia. Fortunately we can steer our destiny toward optimal health even after years of dietary and lifestyle abuse by addressing each identified aging factor. Neutralizing the Effects of Free Radicals Free radicals are unstable electrons produced during the normal course of metabolism, causing damage to the delicate cell nucleus and mitochondria. Free radicals can wreak havoc with DNA, altering its structure and causing [more...]

Date: 2010-10-12 07:34:49

mtd | .:: FitriPDF.com ::. | Manual Owners

Mtd fails to interact with Bcl-2, Bcl-XL, Bax, Bak, and Hrk. A, plasmids expressing Mtd or Bcl-2 fused to the GAL4 DNA-binding … http://www.jbc.org/content/273/15/8705.full.pdf * pdf Mycobacterium Tuberculosis Direct Test (MTD) Information Sheet The Mycobacterium Tuberculosis Direct (MTD) test is now available for select specimens at the … The MTD test is an amplified test for the detection of … http://doh.sd.gov/tb/Documents/MTD.pdf * pdf J. (T.I.), (M.T.D.) (M.C.D.). M.T.D. of each preparation healthy rabbits of about. 2 kilograms … M.T.D. by intramuscular administration the [more...]

Date: 2010-10-13 15:40:29

Best 2010 Ebook Collection Part 4

Dean J.R. - - - Extraction Methods for Environmental Analysis Dean M. - - - The Human ATP-Binding Cassette (ABC) Transporter Superfamily Dean M.W. - - - $30 Music School Dean T. - Gleek J. - - Internet Marketing Secrets Revealed Deans G.K. - Kroeger F. - Zeisel S. - Winning the Merger Endgame: A Playbook for Profiting from Industry Consolidation Deans P.C. - - - E-commerce and M-commerce technologies Deb S. - - - Video data management and information retrieval Deb S. (ed.) - Deb S.P. (ed.) - - p53 Protocols Debarre O. - - - Higher-Dimensional Algebraic Geometry Debevec P. - - - [more...]

Date: 2010-10-11 18:41:15

Preserving The Dna Of Endangered Species With The Natural History ...

Hi all, I wanted to tell you about some of the interesting new work I’ve been doing with the Natural History Museum in London. This new project is really an extension of the studies I’ve been doing in DNA sequencing. My team at Global Research, along with GE Healthcare and the Museum, are now trying to improve methods of obtaining DNA from fresh and preserved historical samples of endangered species (like the giant squid you see below) in order to learn more about them. In the past the team has worked on methods to enzymatically amplify specimen DNA, long term preservation of samples, and [more...]

Date: 2010-08-30 13:56:36

Welcome to Sage

Synthetic genomics is the chemical synthesis of DNA sequences. The synthesized sequences can already exist – as in the chemical synthesis of genes naturally found in an organism – or it can involve the synthesis of novel, unnatural DNA sequences. Synthetic biology is both the design and construction of new biological parts, devices, and systems and the redesign of existing natural biological systems for useful purposes. Because the synthetic life sciences may enable the synthesis of biomolecules, whole genomes, and even simple life forms, these sciences have enormous potential, as they [more...]

Date: 2010-09-16 13:18:45

Evolution News & Views: Does Darrel Falk's Junk DNA Argument for ...

Dr. Falk appears to be too hasty in his assumption that pseudogenes have "no function" and thereby show evidence for common ancestry. The bottom line is that the "junk" or "gibberish" DNA paradigm is being consistently overturned as time goes on, and we now know that the vast majority of our DNA has function. As one news article said when reporting on the results of the ENCODE project in 2007: The findings, from a project involving hundreds of scientists in 11 countries and detailed in 29 papers being published today, confirm growing suspicions that the stretches of 'junk DNA' flanking [more...]

Date: 2010-03-23 19:00:45

Legal DNA Test For Child Support | Law Offices of Charles R. Gueli

If you are a doubtful father, how will you see to it that the claims of the other party are true so you may be willing to give support to the kids? The answer to that should be a settlement. Often, the legal DNA test is enough to settle any argument. However, the processes are different. For one, there are some fathers who do not really demand much or do not doubt the matter at all. The most common situation occurs when after a long time of being together, the parents decided to call their relationship quits. With that, especially if the issue does not really tackle infidelity, the father is [more...]

Date: 2010-09-09 07:47:46

UC San Diego to Lead Personalized Medicine Project on Mood-Stabilizer

The other participating institutions are Indiana University, University of Chicago, University of Iowa, University of Pennsylvania, Johns Hopkins University, University of Michigan, the Translational Genomics Institute in Phoenix, Case Western Reserve University, University of Bergen in Norway, and Dalhouise University in Canada. Research plans call for diagnosing and treating with lithium a total of 700 patients at the ten sites, following the patients’ progress for two years, with a particular emphasis on noting the occurrence of any relapses and the period of time it takes to recover. [more...]

Date: 2010-09-07 17:08:00

IDT in Coralville Named Best Place to Work - Iowa City Area ...

5/27/2009 Ranks in Top 10 of Small Life Science Companies The Scientist, magazine of the life sciences, announced the winners of its 7th annual Best Places to Work in Industry survey. This year's survey yielded responses from representatives of 238 life sciences companies worldwide.  Respondents were asked to assess their working environment according to 45 criteria in 8 different categories, including job satisfaction, benefits, training, and integrity. Room for intellectual and career development, a family-friendly environment, and less hierarchy topped respondent's priorities. [more...]

Date: 2009-05-27 14:10:08

Science Seminar - Ancient DNA

From just a few grams of ancient sediment, it is possible to reconstruct the environment in disparate places at different times. This is possible by extracting total DNA from sediments and looking for the genetic signals of various organisms that contributed to the mix. By comparing the signals with reference databases, identification to genus and species level of plants and animals is often possible. Such information can inform theories of niche stability, extinction dynamics and environmental change, and are therefore complimentary to existing methodologies such as pollen and macrofossil [more...]

Date: 2010-10-06 01:21:01

Tata Aria Car Review | price in India | colors | top speed ...

It is also among those rare cars in India to offer advanced safety features like the Electronic Stability Programme (ESP), which ensures the car’s stability, and six airbags to ensure safety in exigencies. The Aria’s styling is a blend of bold proportions, uncluttered lines and uncompromising aesthetics. The aggressive, well planted stance is tempered by a lunging silhouette and a dynamic roofline. Wraparound dual barreled headlamps complement the signature Tata grille. Chrome detailing on the sides accentuates its premium class. The interiors reflect the same richness and elegance in [more...]

Date: 2010-10-12 11:04:01

Melting point of DNA - Science Forums

Welcome to ScienceForums.Net! We welcome science discussion at all levels — from beginners to researchers, covering topics from biology to computer science, and much more. Registration is fast and free, and allows you to post on the forums, so register now and join the discussions! After you've registered, come in and introduce yourself, or visit the forum index. If you need any help registering, posting, or if you just have some questions about our site, please feel free to contact us at staff at scienceforums dot net. Start new topics and reply to othersSubscribe to topics and forums to [more...]

Date: 2008-02-21 01:27:06

Methods Rapidshare Free Full Downloads with Hotfile and MegaUpload ...

Methods Rapidshare methods Crack Methods Megaupload methods torrent methods serial, Full Versions Rapidshare Direct Results Download Methods from Rapidshare Crack Torrent Download. View the search links and direct download below. methods is available on a new fast direct download service with over 2,000,000+ Files to choose from. Download anything with more then 1000+ Kb/s downloading speed. Signup process takes just 10 sec to go. Signup today and enjoy the speed [more...]

Date: 2010-10-11 16:03:46

Dr. Pandey “pulls off” new DNA sequencing method

The current method to sequence DNA is called gel-electrophoresis. This process is very costly and time consuming. However, Dr. Pandey and his research team have proposed a way to make DNA sequencing far easier and cheaper than the current method. For those of you scratching your heads and wondering what the purpose of DNA sequencing could possibly be, imagine in the futurew going to the doctor and getting customized medicine just for you based on your DNA. This medicine would do exactly what it needed to in order to fix your problem because it was made specifically for you and only you. [more...]

Date: 2010-09-23 17:36:11

Study Says Nutrition And Heredity Are Genetically Linked

About News News From All Over The World, Right To You ... View News's Profile A challenging goal in biology is to understand how the principal cellular functions are integrated so that cells achieve viability and optimal fitness under a wide range of nutritional conditions. Scientists from the French research centers INRA and CNRS showed by genetic approaches that, in the model bacterium Bacillus subtilis, central carbon metabolism (which generates energy from nutrients) and replication (which synthesizes DNA), two key functions in the fields of nutrition and heredity, are tightly linked. [more...]

Date: 2007-05-16 07:00:00

Could taking a nutritional supplement keep you young?

It might be more real than not. Scientists working at the biotech giant Geron Corporation have isolated a molecule, TA-65, which has the ability to increase telomerase activity (It is important to note that his find has not been evaluated in published peer reviewed studies as of yet). This is an exciting breakthrough for telomerase is an enzyme that adds DNA sequence repeats to the 3′ end of DNA strands in the telomere regions, which are found at the ends of eukaryotic chromosomes. The telomeres contain condensed DNA material, giving stability to the chromosomes. These telomere DNA [more...]

Date: 2010-07-14 19:14:34

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