Our blogs and videos choice: dna sequencing pcr

Estrogen-Receptor Polymorphisms and Effects of Estrogen ...

Sequencing products were analyzed on a 3700 DNA Analyzer. DNA sequencing data were aligned and analyzed with the use of DNA-analysis software (Sequencher, Gene Codes, Ann Arbor, Mich.). Details about the sequencing primers and the PCR and ..... Furthermore, their influence on other clinical effects of estrogen, including the relief of perimenopausal symptoms and the effects on the risk of osteoporosis, venous thrombosis, and breast cancer, must also be evaluated before a [more...]

Date: 2002-03-28 05:00:00

Blog posts (58) | Videos (20)
 


How Fingerprinting Helps In DNA Paternity Testing

This form of fingerprinting method is also known as DNA profiling. Using this technique, the individuals are identified on the basis of their unique DNA profiles. This DNA profiling is done by analyzing the VNTR sequences. It stands for variable number tandem repeats. This sequence is found to be different in individuals except in twins. As per the facts mentioned above, the DNA samples of the child will exhibit the similar VNTR loci as that of their parents. This fingerprinting helps in identifying that whether the known father of a child is genuinely his biological dad. In fact, it is [more...]

Date: 2010-08-09 11:41:10


Case Analyst (Salt Lake City)

Successful candidate will perform genotyping analysis. Experience using GeneMapper genotyping software a plus. Experience performing DNA extraction, PCR and genotyping for STR analysis and mitochondrial sequencing essential. Other lab duties as directed. Required: • BS in Biochemistry, Molecular Biology, Biology or related field • 1 year experience in laboratory • Knowledge of molecular biology testing, analysis and reporting. • Must be able to use hands, stand for periods of time and lift up to 20 lbs. Preferred: • Computer and Microsoft applications proficiency • 2 years [more...]

Date: 2010-10-01 04:43:12


Free molecular biology software for Macs | Bioinformatics Blog

Posted on the October 12th, 2010 under Free mac software by bioinformatics-blog 1. Serial Cloner Serial Cloner is fantastic all-in-one workbench; import and manipulate sequences, construct plasmid and restriction site maps, determine %GC and fragment TM, extract and ligate fragments, perform virtual PCR… and lots more, all in one window using a very intuitive graphical interface. 2. 4Peaks 4Peaks is an extremely user friendly DNA sequence chromatogram viewer and editor from the extremely talented guys at Mekentosj. It’s miles better than any of it’s clunky counterparts… try it, [more...]

Date: 2010-10-12 11:08:47


Evaluation of Five Methods for Total DNA Extraction from Western ...

DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. Methodology/Principal Findings From individual western corn rootworm beetles, Diabrotica virgifera virgifera, DNA extractions by the SDS method, CTAB method, DNAzol® reagent, Puregene® solutions and DNeasy® column were compared in terms of DNA quantity and quality, cost of materials, [more...]

Date: 2010-08-13 07:00:00


sequencing of short DNA fragments (40-240bp) - SEQanswers

Hello together, We would like to prepare barcoded fragment libraries of enzymatically digested genomic DNA. The fragment size range we are interested in is 40 to 240bp. We want to sequence 50bp using a SOLiD 4 machine. My questions are: 1) What will happen if e.g. a 40bp fragment is sequenced? Will sequencing continue into the internal adaptor? There are various blocking oligos in the sequencing kit, and I am not entirely sure about their purpose. If they block the complete internal adaptor sequence, does this mean that only 34bp will be sequenced (I believe the fluorescent oligos are [more...]

Date: 2010-08-31 11:57:47


"Polymerase Chain Reaction (PCR)" Biology Animation Library ...

"Polymerase Chain Reaction (PCR)" Biology Animation Library :: Dolan DNA Learning Center: "Polymerase Chain Reaction Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA. This animation is featured in our 'Spotlight Collection' on Polymerase Chain Reaction, along with video interviews with Kary Mullis, a 3D molecular animation of PCR, and several laboratory [more...]

Date: 2010-08-23 17:54:00


Efavirenz Plasma Concentrations and Cytochrome 2B6 Polymorphisms ...

Genomic DNA was extracted from the peripheral whole blood of each subject with a DNA extraction kit (Qiagen, Valencia, CA). CYP2B6 polymorphisms were genotyped by resequencing using primers and polymerase chain reaction (PCR) conditions described by Lamba and associates.15 Sequencing was carried out with an ABI Prism 3700 Automated Sequencer using the PCR primers or internal primers (sequence available on request). Sequences were assembled using the Phred-Phrap-Consed [more...]

Date: 2010-09-22 19:28:38


TriMark Publications Announces Release of Its Nutraceuticals and ...

Packed with the latest information relating to new products and industry trends, this analysis not only quantifies but also qualifies the nutraceuticals market in the areas of research, product development and investment opportunities. ... DNA Sequencing and PCR Markets - Genomics World Markets - High-Growth Diagnostic Tests Markets - Indian Pharmaceutical Industry - Key Diagnostic Testing Markets - Mammography World Markets - Medical Imaging Markets - Microarray Markets [more...]

Date: 2010-10-11 21:42:02


Heliscope builds world's fastest DNA sequencer | Singularity Hub

The Short: Technology Review reports that for just $1,350,000 USD you can have have the worlds fastest commercially available DNA sequencer, called the Heliscope. The machine developed by Helicos BioSciences takes just one hour to read 1.3 billion base pairs from a strand of DNA. Here is a picture of the beauty: HeliScope™ Single Molecule Sequencer The Long: The Heliscope is being marketed as a DNA microscope. It is unique in the field of DNA sequencing because it sequences an actual DNA strand whereas most other sequencing technologies use PCR to create millions of copies of an original [more...]

Date: 2008-07-14 16:58:33


Photo Irl | Stadium Sports Central

... features can be built to expand the range of PCR-based applications. PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Practical Approach, but sets out to address some of the new and exciting applications of PCR including direct sequencing, mRNA quantitation and expression, genomic DNA mapping, and mutation analysis. .... Irl Allison Library. Arranged by Irl Allison. This edition: 9569. Willis. Book only. 32 pages. Published by Willis Music. [more...]

Date: 2010-10-12 22:37:02


Acinetobacter baumannii in Localised Cutaneous Mycobacteriosis in ...

Moreover, in 13 samples Acinetobacter baumannii was detected by PCR and proven by DNA sequencing. Clinical features included highly elevated WBCs, heterophilia, lymphocytopenia, monocytosis, severe anemia and weight loss. ..... P. C. Schreckenberger, M. I. Daneshvar, R. S. Weyant, and D. G. Hollis, “Acinetobacter, Achromobacter, Chryseobacterium, Moxarella, and other nonfermentive gram-negative rods,” in Manual of Clinical Microbiology, P. R. Murray, Ed., pp. [more...]

Date: 2007-12-29 08:00:00


GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...

GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are  involved in various disease problems in humans and aquatic  animals and are known to be  phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific  diagnostic test is warranted for detection and characterization of this pathogen.In the present study,  Fish  and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]

Date: 2010-10-08 20:08:05


PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading

Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]

Date: 2009-11-13 08:00:00


DNA method gives new perspective on the Mysteries of Nature

We demonstrate that this new approach enables the assignment of virtually all the generated DNA sequences to the correct source once sequencing anomalies are accounted for (miss-assignment rate less than 0.4%). Therefore, the method enables accurate sequencing and assignment of homologous DNA sequences from multiple sources in single high-throughput GS20 run. We observe a bias in the distribution of the differently tagged primers that is dependent on the 5' nucleotide of the tag. In particular, primers 5' labelled with a cytosine are heavily overrepresented among the final sequences, while [more...]

Date: 2007-02-15 17:01:00


ISPUB - Comparison and Development of A Rapid Extraction Methods ...

Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological, Archaeological, Museum, Medical and Forensic Specimens. New York, NY: Springer Verlag. (s)7. Holland, M.M. and T.J. Parsons. 1999. Mitochondrial DNA sequence analysis— validation and use for forensic casework. Forensic Science Review. 11: 22 – 49. (s)8. Matysiak-Scholze,U., Dimmeler,S. and Nehls,M. (1996) Technical Tips Online, T40011. (s)9. Near T.J., Bolnic D.I. and Wainwright P.C., Fossil calibrations and molecular divergence time estimates in centrarchid fishes, Evolution, 2005, 59(8): 1768-1782 (s)10. Paabo [more...]

Date: 2010-06-06 01:06:13


Genome Biology | Full text | Optimization and clinical validation ...

Novel features of this platform include the development of a robust algorithm that accurately predicts PCR bias during DNA amplification and can be used to improve PCR primer design, as well as a powerful statistical concept for inferring .... This probability is reduced when intra-primer hairpin formation is predicted, and increased according to degree of complementarity between tag sequence and viral sequence. In this manner, the probability that each nucleotide will be [more...]

Date: 2007-05-27 00:00:00


Graduate/Postdoctoral researcher in Ravenna- University of Bologna ...

DNA extraction, - PCR amplification of mitochondrial / nuclear loci – sequencing – database management, - troubleshooting during laboratory analyses. Candidates should have a Master's Degree in biological science or related fields. [more...]

Date: 2010-08-11 09:00:43


Research assistant, DNA hash pooling | MetaFilter Jobs

... project (www.nanoinfobio.org), the appointed Research Assistant will be responsible for experimental validation of this technique using molecular biology methodologies such as DNA extraction, PCR, RFLP and automated sequencing. [more...]

Date: 2010-08-06 00:24:19


Intellectual Property Expert Group (ipeg) » Blog Archive » Are the ...

These are just two recent cases in the US but the implication on DNA sequence patents may be far reaching. Back in 2000, when the new economy was still alive, there was a lot of focus on DNA sequence patents. A lot of companies were very cautious and a lot of genetic test developments were stalled because of patent issues. When asking an expert you could get philosophical answers: either you licence or you ignore or you litigate. Now in 2000 there were still patents from the early days of DNA sequencing around. Some of these patents might have been inventive as the inventive step was a lot [more...]

Date: 2010-09-20 16:55:26


Oligonucleotide Synthesis: Methods and Applications (Methods in ...

Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene [more...]

Date: 2004-07-30 07:00:00


The Quality Stocks Stock Newsletter For Smallcap Companies Blog ...

Shivji noted the 15 years of genomic research and leadership experience of Dr. Schroth, and his tireless efforts to advance gene expression techniques using DNA sequencing, microarrays, PCR and next-gen sequencing, all of which has resulted in numerous publications and patents spanning the entire field of genomic analysis. Dr. Schroth expressed his excitement over the opportunity to push the SmartChip platform forward as a perfect solution for multiple global research efforts that are focused on making improved and more targeted therapeutics, and called the SmartChip system “the most [more...]

Date: 2010-10-12 17:57:42


Technology Review: A Portable DNA Detector

A new portable DNA analyzer performs real-time analysis of blood samples left at the scene of a crime. Researchers at the University of California, Berkeley, developed the device, which packs microfluidics, electronics, optics, and chemical detection technology into a single briefcase-sized unit. "While previous groups have developed lab-on-a-chip systems, none of them have succeeded in making a completely portable, robust system that can be used at a scene," says team leader Richard Mathies. On-site CSI: After a blood sample is collected from the scene of a crime, this briefcase-sized [more...]

Date: 2008-09-24 04:00:00


New DNA Diagnostic Test For Lyme Disease May Have Merit

Milford Hospital in Massachusetts has been working for over a year on a new type of diagnostic test for Lyme disease using DNA sequencing rather than DNA nesting. Huh? We have all heard of the polymerase chain reaction (PCR) method of [more...]

Date: 2010-09-17 03:38:41


Part-Time Lab Technician – Great For Students (RTP) | PCR Jobs

Basic laboratory skills (emphasis on pipetting) - A good driving record. Job duties include, but are not limited to: - Driving to customer sites in a company-provided car to pick-up DNA samples - Processing DNA sequencing reactions [more...]

Date: 2010-10-11 18:43:41


Lab Tech / Research Associate (Torrey Mesa) | PCR Jobs

Candidate must be familiar with basic molecular biology techniques such as DNA purification, DNA gel electrophoresis, restriction digestion, PCR including primer design, plasmid construction, DNA sequence analysis software and bacterial transformation. Candidate will also assist with sample receipt and tracking, maintain inventory and equipment, and perform general lab maintenance and cleaning to include handling of biological waste. Education/ Skills/Attributes Required: · Must be a US Citizen. · BS degree in biology/biochemistry with 0-3 years industrial experience. · Experience with [more...]

Date: 2010-10-12 19:15:31


Sequenziamento del DNA- Prima generazione (metodo Sanger) « my GenomiX

Così, ecco la prima parte della mia serie sul DNA sequencing: oggi tratterò la prima generazione, vale a dire il metodo Sanger. Innanzitutto facciamo un piccolo ripasso sul DNA. Il DNA (o acido deossiribonucleico) è una molecola che ha l’aspetto di una doppia elica formata da due catene di nucleotidi. I nucleotidi sono piccole molecole costituite da un gruppo fosfato, uno zucchero (deossiribosio) e una base azotata; mentre le prime due componenti sono sempre uguali e costituiscono l’ossatura della doppia elica, le basi azotate esistono in quattro “versioni” differenti. Esse si [more...]

Date: 2010-09-08 08:31:06


CIENCIASMEDICASNEWS: Recurrent Granulibacter bethesdensis ...

DNA was isolated from human tissue by using the Maxwell 16 Tissue DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. DNA concentrations were measured by using a UV spectrophotometer (NanoDrop, Wilmington, DE, USA). The 16S rRNA and methanol dehydrogenase subunit 1 (GeneID YP_744165.1) genes of G. bethesdensis were analyzed by using a PCR and primer sequences 16S-forward: 5'-TCGGGTGGGCACTCTAAAGG-3', 16S-reverse: 5'-GCATCACTGCCTAGCTTCCC-3', MDH-forward: 5'-CCGCAATACGGTCAATTCG-3', and MDH-reverse: 5'-GCCGATCTTCCAGGTTTCTTC-3'. Each reaction mixture [more...]

Date: 2010-09-02 23:42:00


Epigenetics Papers: A Genomic Sequencing Protocol that Yields a ...

Genomic sequencing protocols, which have been developed to ascertain the methylation status of selected regions within genes, utilize the Maxam and Gilbert chemical cleavage reactions [. ... To address these problems, we have developed a genomic sequencing method that provides positive identification and localization of 5-methylcytosine in genomic DNA. The method is based on sodium bisulfite-mediated conversion of cytosine to uracil in single-stranded DNA, followed by PCR [more...]

Date: 2007-03-13 21:09:00


DNA Gel Extraction Kit

The DNA Gel Extraction Kit is designed for the rapid purification of DNA fragments from agarose gels. The kit allows for the removal of agarose, enzymes, dNTPs, ethidium bromide, dyes and detergents from the DNA fragment. The recovered DNA is of a high quality, and is suitable for a number of downstream applications including restriction enzyme digestions, ligations, labeling, hybridizations and sequencing. The kit employs the use of a proprietary resin for DNA purification. This resin is an extremely inert and stable substance and is able to preferentially bind nucleic acids in the presence [more...]

Date: 2010-03-05 04:40:00


University Of Pune SYLLABI OF BACHELOR OF PHARMACEUTICAL SCIENCES ...

DNA Hybridization, Blotting techniques, polymerase chain reaction (PCR) Site directed mutagenesis, Restriction Fragment Length Polymorphism (RFLP), Human Gene therapy, DNA fingerprinting. Gene synthesis & gene machine, Gene sequencing [more...]

Date: 2010-09-02 07:32:33


Thrombosis Journal | Full text | Expression of sterol regulatory ...

DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]

Date: 2009-02-18 08:00:00


Colony PCR: Another Use For Toothpicks « Promega Connections

Colony PCR: Another Use For Toothpicks July 26, 2010 by Isobel I still remember my JOY (sadly, yes) when I first learned about colony PCR. It allowed me to avoid a laborious procedure involving genomic DNA isolation, restriction digestion, and (the dreaded) Southern blotting. I was trying to show definitively that a transposon had successfully been inserted into a target gene. Using colony PCR, I could just amplify the DNA from a colony and then show that the transposon increased the size of the expected PCR product compared to a control. Joy. I could also use colony PCR to screen DNA [more...]

Date: 2010-07-26 15:13:31


iGEM UT-Tokyo(東大)実験ノート - 9/2 purification of DNA by ...

Experimenter Kariyazono, Nakatake, Zen Abstract purification of location sequence(digested by X & P on 9/2) by electrophoresis & extraction of bands from the gel Protocols 1.separating of DNAs through electrophoresis ←direction sample1 sample2 sample3 sample4 nothing(for 3 lanes) sample3′ sample4′ sample1′ 2.Extraction of DNAs Result;consentration of DNA sample1 5.3ng/ul sample2 7.8ng/ul sample3 6.9ng/ul sample4 7.3ng/ul sample1′ 7.8ng/ul sample3′ 5.8ng/ul sample4′ 6.8ng/ul flpe(PCR②) 3.6ng/ul stored in the [more...]

Date: 2010-09-02 04:27:41


DNA Art Blog » Blog Archive » PCR Process

We use primer sets that are optimally designed to span the specific genes you choose to have isolated for your canvas. Our technique focuses on the prevention of pre-PCR mis-priming and primer dimerization. Our primers are designed using software with algorithms that check for the potential of secondary structure formation and annealing of primers to itself or within primer pairs. We use hot start Taq Polymerase is designed to enhance the specificity, sensitivity, and yield of the target DNA sequence. Following PCR amplification and purification of the template DNA, we use restriction [more...]

Date: 2010-10-12 15:19:03


NedayeFan Co

T4 Polynucleotide Kinase 500 u EK0031 450,000 T4 DNA Ligase (With PEG) 200 u EL0335 450,000 T4 DNA Ligase 200 u EL0015 450,000 T4 DNA Polymerase 100 u EP0061 440,000 Taq DNA Polymerase (native) 100 u EP0281 470,000 Taq DNA Polymerase (native) 500 u EP0282 1,880,000 Taq DNA Polymerase (recombinant) 100 u TA7505C 50,000 Taq DNA Polymerase (recombinant) 5,000 TA7507C 1.200,000 Taq DNA Polymerase (recombinant) 500 u TA7506C 160,000 TaqI 3,000 u RE7717C 230,000 TBE Buffer, 5X 50 ml MR7725C 20,000 Total Protein (Automatic and manual) 300Tests BK8145C 25,000 Triglycerides (Automatic and manual) New [more...]

Date: 2005-10-25 10:14:00


genomic DNA PCR protocols and methods

Research news, bioproducts, protocols, reagents, forums genomic DNA PCR > Can someone forward me a general protocol for doing PCR using genomic > DNA as the template? I seem to recall needing about 100 ng of DNA, but > I forget stuff like how much Taq to use, concentration of dNTP's, and > concentration of primers. General protocol for PCR Each person uses a slightly different ratio of reagents. For DNA amplicons up to 500 bp, use 25 uL reaction volumes as follows: On ice add to thin-walled pcr tubes 0.125 uL Taq polymerase (1.25 U, Gibco) 2.5 uL of 10 x PCR buffer minus Mg [more...]

Date: 2004-12-27 00:00:00


LAB TECH – DUKE – Molecular biology/evolution/genomics (Durham, NC ...

DUTIES: Responsibilities will include: DNA and RNA extractions, sample prep for Illumina library construction and sequencing, standard and quantitative real-time PCR, maintenance of live insects in the lab, as well as lab organization, ordering, and management. Experience with any or all of the above is desirable. The first few weeks of the position will involve helping to set up the lab (ordering, etc). Depending on skills and interests, opportunities could also include genome sequence analysis, use of molecular databases, molecular phylogenetics, design of PCR and sequencing primers, field [more...]

Date: 2010-10-11 18:43:41


X-Tagged.com Adult Social Bookmarking - DNA sequencing

PCR Analysis facilitates the capacity to make millions of DNA copies from a single biological sample. This type of analysis enables the forensic scientist to examine small data such as a few skin cells. It also lessens the chances of contamination of the sample, but it requires very meticulous work. Short tandem repeat (STR) technology Short tandem repeat (STR) technology analyzes all 13 Loci (regions )for DNA testing and is the technology used by the FBI databases at the local, state, and federal level. Short tandem repeat (STR) technology for DNA profiles from convicted offenders, unsolved [more...]

Date: 2010-10-12 18:10:35


PCR Rap | sciencerapper

An ingenious technique called Polymerase Chain Reaction (PCR, in the labaratory vernacular) uses a Polymerase to selectively an cheaply amplify certain sequences of DNA from very small amounts of starting materials.  The invention of PCR piggy-backe don many existing technologies, and we have used PCR to learn many, many things about biology. The following rap about the Polymerase Chain Reaction that highlights a few things: the beautiful way in which DNA is copied (using enzymes called Polymerases) the great insight of Kary Mullis to create a useful tool that could copy DNA in monumental, [more...]

Date: 2010-09-04 14:18:04


ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...

Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]

Date: 2010-08-25 00:51:13


Forensic Serology Information Page

What is Forensic Serology?According to forensic serologist Marcella Jones of www.ForensicMentor.com, forensic serology is the analysis of body fluids as they relate to forensic cases, including DNA analysis. Accordingly, the role of the forensic serologist involves:Examining evidence for the presence of body fluids e.g. blood, semen, hair, tissue, saliva, feces, and urineEvaluating evidence for potential DNA analysisEvaluating species of body fluidsPerform PCR (polymerase chain reaction) based typing of STR (short tandem repeat sequences) of genomic DNA, or mitochondrial sequence analysis of [more...]

Date: 2010-02-04 08:00:00


ERV's latest blog on the FDA paper. One step too far?.

PNAS paper, using standard PCR to ID 'positives' and 'negatives', with insane numbers of bands as a result of non-specific primer binding. Insane numbers. As if this is 1990 and we dont know what 'Real Time PCR' and 'Taqman Probes' are. Im going to ignore that and grant the premise they really have IDed viral sequences. And they arent XMRV. They dont cluster anywhere near XMRV on a phylogenetic tree. Theyre something else entirely. Okay, well, logically it could be contamination from mouse DNA. So they checked for that... by looking for mouse mitochondrial DNA. *blink* I understand [more...]

Date: 2010-08-23 07:00:00


PLoS ONE: Evidence of Authentic DNA from Danish Viking Age ...

Linea Melchior1, Toomas Kivisild2, Niels Lynnerup3, Jørgen Dissing1*1 Research Laboratory, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark, 2 Leverhulme Center for Human Evolutionary Studies, The Henry Wellcome Building, University of Cambridge, Cambridge, United Kingdom, 3 Laboratory of Biological Anthropology, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark Abstract Top Background Given the relative abundance of modern human DNA and the inherent impossibility for incontestable proof of authenticity, results obtained on ancient [more...]

Date: 2008-05-28 07:00:00


Blood test detects HIV/AIDS in infants aged 6 months in India DNA ...

Unlike other tests, the HIV DNA PCR test does not rely on the presence of antigens or antibodies in the blood for diagnosis. Instead, it identifies genetic material by highlighting sequences of the virus within the subject’s DNA. All babies born to mothers with HIV are born with HIV antibodies. The currently available tests can predict the chances of HIV infection in infants borne to infected mothers only after the completion of 18 months. This delay in diagnosis often caused a problem for doctors because they found starting the treatment using the life-saving Anti-Retroviral Treatment [more...]

Date: 2010-07-19 22:55:54


Plasmid double locus sequence typing for IncHI2 plasmids, a ...

Plasmid double locus sequence typing for IncHI2 plasmids, a subtyping scheme for the characterization of IncHI2 plasmids carrying extended-spectrum β-lactamase and quinolone resistance genes Aurora García-Fernández and Alessandra Carattoli* Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy *Corresponding author. Tel: +39-06-49903128; Fax: +39-06-49387112; E-mail: alecara{at}iss.it Received October 9, 2009. Revision requested January 25, 2010. Revision received February 26, 2010. Accepted March 4, 2010. Abstract Objectives IncHI2 [more...]

Date: 2010-03-31 07:00:00


E. Coli Plasmid Vectors: Methods and Applications (Methods in ...

E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) (archive) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, N.J.), V. 235.) Table of Contents The Function and Organization of Plasmids Finbarr Hayes Choosing a Cloning Vector Andrew Preston Escherichia coli Host Strains Nicola Casali Chemical Transformation of E. coli W. Edward Swords Electroporation of E. coli Claire A. Woodall DNA Transfer by Bacterial Conjugation [more...]

Date: 2004-04-04 08:00:00


Research Associate (San Diego) | PCR Jobs

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:  Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.  Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.  Prepares technical reports, summaries, protocols and quantitative analyses.  Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]

Date: 2010-10-01 19:07:24


Research Technician II (Durham) | PCR Jobs

Three years of molecular biology research and experience in the following is preferred: DNA/RNA extraction; DNA sequencing; PCR/ Real Time PCR; Taqman; gel electrophoresis; and tissue culture (propagation and storage). Computer proficiency in MS office programs, Photoshop, and basic statistical programs is also preferred. Qualified candidates should email a resume to the “reply to” email above. Please note “Tech II” in the subject line. Duke University is an Equal Opportunity/Affirmative Action Employer. Topics: Raleigh-Durham PCR jobs | No Comments [more...]

Date: 2010-10-11 18:43:40


Methylation Test May Aid Colon Cancer Diagnosis (CME/CE) | Discuss ...

To develop a more sensitive test for DNA methylation, investigators employed a form of technology known as BEAMing (beads, emulsion, amplification, and magnetics). They performed PCR amplification of individual DNA fragments covalently linked to magnetic beads suspended in water-based nanoparticles immersed in oil droplets. The beads contained a DNA sequence specific for exon 1 of the vimentin gene, which is hypermethylated in colon cancer. Following PCR amplification, the beads were hybridized by means of fluorescent probes specific to the state of methylation and then sorted and analyzed by [more...]

Date: 2010-10-09 11:00:27



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