EpiCentral: Examining blocking lesions in ancient DNA
Blocking lesions prevent amplification and sequencing of affected molecules, thus limiting the analysis of DNA derived from ancient samples. Heyn et al. recently developed a new method--polymerase extension profiling (PEP)--that reveals occurrences of polymerase stalling on DNA templates. This sequencing-based technology allows detection of damage on a single-molecule level. The technique used CircLigase™ ssDNA Ligase for high-efficiency ligation of single-stranded adaptors (containing the Roche 454 A sequence) to the 3’ ends of primer-extension products. The authors found evidence of [more...]
Date: 2010-10-07 20:29:00
Official launch of iBOL activates world's largest biodiversity ...
Toronto – The largest biodiversity genomics initiative ever undertaken – an international effort to build a digital identification system for all life on Earth – will be officially activated this week. The International Barcode of Life Project (iBOL) will be launched by the Minister of Research and Innovation, Glen Murray, during an event at Toronto’s CN Tower on Saturday, September 25 at 6:30 p.m. By enabling automated, rapid and inexpensive species identifications, iBOL will transform biodiversity science and its applications throughout society. “We are witnessing alarming rates [more...]
Date: 2010-09-24 13:22:46
Learn The Basic Process Of DNA Paternity Testing
However, how does DNA testing work? The DNA Paternity Test is conducted by taking DNA sample of the supposed father and the child. The child has a combination of the mother and father’s DNA that is a known fact. Thus, the child’s genome will contain almost equal parts of the mother and the father. The proof of paternity is obtained by comparing the DNA sequences of both the child and the supposed father. If their DNA results match, it means that the supposed father is indeed the child’s biological father. If it doesn’t, then it sets things clear and gives peace of mind to both [more...]
Date: 2010-10-08 15:53:44
Ph.D or post-doc assistantship in plant evolution/biogeography at ...
The successful applicant will choose one or more of these plant families to: generate flow cytometry data aimed at determining ploidy levels; generate nuclear DNA sequences and infer species trees; incorporate ecological data to investigate issues of niche conservatism vs. niche evolution in the selected groups. Requirements: Demonstrated experience in molecular, phylogenetic and flow-cytometry methods and/or ecological niche modeling will be highly valued in the selection process. Excellent knowledge of the English language, written and oral, required. Familiarity with additional European [more...]
Date: 2010-06-04 13:05:00
Sandwalk: Nobel Laureate: Walter Gilbert
The Nobel Prize in Chemistry 1980. "for their contributions concerning the determination of base sequences in nucleic acids" Walter Gilbert (1932 - ) was awarded the Nobel Prize for developing a chemical method of sequencing DNA (with Allan Maxam). The method relied on chemical reactions that cleaved DNA at specific residues. By carrying out partial reactions where only one cleavage occurred in each DNA strand, it was possible to separate the cleavage products on an acrylamide gel and determine the position of each residue by the length of the fragment. The chemical sequencing strategy has [more...]
Date: 2008-12-10 14:35:00
HHMI News: Timothy Harris to Deliver Public Talk at Janelia Farm
The event is free and open to the public, but tickets are required for admission. Directions for obtaining the tickets are available on the HHMI web site at www.hhmi.org/janelia/events.html. Seating is limited to 250 people. The lecture is the seventh in a series called “Dialogues of Discovery at Janelia Farm.” Past speakers in the series have included Thomas R. Cech, former president of HHMI and an HHMI investigator at the University of Colorado, Boulder; Roian Egnor, a fellow at the Janelia Farm Research Campus; Ronald M. Evans, an HHMI investigator at the Salk Institute for Biological [more...]
Date: 2010-10-04 17:01:00
Bio-hackers? Biology Is Technology: Robert H. Carlson
DNA for these purposes is synthesized either in the lab where it is to be used or elsewhere in a dedicated facility and delivered by express mail. Mail-order oligonucleotides were used in 2002 to build a functional poliovirus genome from constituent molecules for the first time. Since then constructs of ever-increasing length have been announced, and a full bacterial chromosome was recently synthesized from short oligos.”This capability so far ahead of what I would have thought possible. The author claims that the technology is moving forward in a fashion consistent with the famous [more...]
Date: 2010-10-10 04:54:00
MEGA: A biologist-centric software for evolutionary analysis of ...
MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences Sudhir KumarSudhir Kumar is conducting large-scale analysis of genome sequences and spatial patterns of gene expression, and developing statistical methods and bioinformatics tools. He is the Director of the Center for Evolutionary Functional Genomics at Arizona State University, AZ, USA. , Masatoshi NeiMasatoshi Nei is one of the founders of molecular evolutionary genetics and pursues statistical analysis of molecular and genome evolution. He is the Director of the Institute of Molecular Evolutionary [more...]
Date: 2010-09-02 23:38:15
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are involved in various disease problems in humans and aquatic animals and are known to be phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific diagnostic test is warranted for detection and characterization of this pathogen.In the present study, Fish and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]
Date: 2010-10-08 20:08:05
Whole-Genome Sequencing in a Patient with Charcot–Marie–Tooth ...
DNA-sequencing methods that involve chemical assays other than the traditional Sanger dideoxy-chain-termination method. Next-generation-sequencing methods produce much larger quantities of data at less expense, but the individual raw [more...]
Date: 2010-03-31 20:55:45
Genetic Technologies Ltd.?s Non-coding DNA Patent Upheld on ...
s (GTG's) patent for non-coding DNA technologies. U.S. Patent No. 5,612,179 titled "Intron sequence analysis method for detection of adjacent locus alleles as haplotypes" claims a method for facilitating the detection of specific gene variations. The third party initiated ex parte re-examination Control No. 90/010,318, was filed on October, 15, 2008 requesting that each of claims 26-32 be found invalid in view of a new reference, which was not previously cited in the prosecution of the '179 patent; namely a printed publication titled "Comparison of Multiple HLA-A Alleles at the [more...]
Date: 2010-07-13 16:50:00
DNA sequencing » Blog Archive » DNA Sequencing Methods
DNA Sequencing – How DNA Sequencing Works – Methods for … An explanation of how DNA sequencing works. … Typically, the automated sequencing method is only accurate for sequences up to a maximum of about 700-800 base-pairs in length. … DNA Sequencing Simulation (Sanger Method) Within each reaction mixture are the following items: a DNA segment of unknown … The other DNA sequencing method (named for Allan Maxam and Walter … Next-Generation DNA Sequencing Methods 921k – Adobe PDF – View as html generation DNA sequencing technologies highlight the striking impact … [more...]
Date: 2010-09-28 04:29:58
Reveo's direct DNA sequencing using tunneling microscopy « The ...
Reveo is developing an ambitious technology to stretch out and deposit taut DNA on conductive surfaces for electronic base detection using one or more STM tips and tunneling current measurements. The linearization and deposition of nucleic acid sequences will likely be done using molecular combing. Reveo’s approach requires atomically flat and positively charged substrate surfaces (e.g. self-assembled monolayers on gold substrates or treated graphite substrates). In addition to molecular combing, Reveo has proposed other methods to linearize DNA, including electrophoretic and hydrodynamic [more...]
Date: 2010-08-25 17:49:12
Gene Patenting — Is the Pendulum Swinging Back? — NEJM
Ultimately, thousands of patents were awarded on different parts of the human genome sequence; reportedly, about 20% of human gene DNA sequences are currently patented. Myriad was founded in 1991 by Walter Gilbert, who won a Nobel Prize for ... Myriad's patents, the earliest of which expire in 2014 and 2015, cover isolated gene sequences, as well as methods of “analyzing” and “comparing” the gene sequences to determine whether the mutations conferring an increased risk of [more...]
Date: 2010-05-19 20:55:59
Keiichi Itakura Biography, life and Career Facts, Invented
After completing his doctoral work, Itakura received a fellowship to study DNA synthesis under Dr. S. Narang at the National Council of Canada, located in Ottawa. Itakura found DNA synthesis to be a compelling field of research and was interested in learning more about the recently discovered synthesized gene of transfer ribonucle-ic acid (t-RNA), which had been found by the Indian-born American biochemist HAR GOBIND KHORANA. After his fellowship ended, Itakura went to the California Institute of Technology in Pomona to work as a senior research fellow. In 1975 Itakura joined the staff of the [more...]
Date: 2010-09-30 10:10:52
Colony PCR: Another Use For Toothpicks « Promega Connections
Colony PCR: Another Use For Toothpicks July 26, 2010 by Isobel I still remember my JOY (sadly, yes) when I first learned about colony PCR. It allowed me to avoid a laborious procedure involving genomic DNA isolation, restriction digestion, and (the dreaded) Southern blotting. I was trying to show definitively that a transposon had successfully been inserted into a target gene. Using colony PCR, I could just amplify the DNA from a colony and then show that the transposon increased the size of the expected PCR product compared to a control. Joy. I could also use colony PCR to screen DNA [more...]
Date: 2010-07-26 15:13:31
The Picasso of DNA
Church thrived in Gilbert's lab. In 1977 he developed a way of automating a key step of Gilbert's DNA sequencing method. DNA strands are made up of combinations of four bases, molecules that are denoted by the letters A, T, C, and G. [more...]
Date: 2010-07-22 20:46:05
Lab Tech / Research Associate (Torrey Mesa) | PCR Jobs
Candidate must be familiar with basic molecular biology techniques such as DNA purification, DNA gel electrophoresis, restriction digestion, PCR including primer design, plasmid construction, DNA sequence analysis software and bacterial transformation. Candidate will also assist with sample receipt and tracking, maintain inventory and equipment, and perform general lab maintenance and cleaning to include handling of biological waste. Education/ Skills/Attributes Required: · Must be a US Citizen. · BS degree in biology/biochemistry with 0-3 years industrial experience. · Experience with [more...]
Date: 2010-10-12 19:15:31
genomic DNA PCR protocols and methods
Research news, bioproducts, protocols, reagents, forums genomic DNA PCR > Can someone forward me a general protocol for doing PCR using genomic > DNA as the template? I seem to recall needing about 100 ng of DNA, but > I forget stuff like how much Taq to use, concentration of dNTP's, and > concentration of primers. General protocol for PCR Each person uses a slightly different ratio of reagents. For DNA amplicons up to 500 bp, use 25 uL reaction volumes as follows: On ice add to thin-walled pcr tubes 0.125 uL Taq polymerase (1.25 U, Gibco) 2.5 uL of 10 x PCR buffer minus Mg [more...]
Date: 2004-12-27 00:00:00
PLANT TISSUE CULTURE TECHNIQUE AND PCR TECHNOLOGY
SSR assays require a minimal amount of genomic DNA. Most of the crop improvement programs are confined to evaluation and selection of naturally occurring clonal variations. In vitro culture techniques provide an alternative means of plant propagation and an important tool for crop improvement. The in vitro cultures are also of low risk for genetic variation since it is more resistant to genetic changes while occurrence of cell division in vitro condition. Gel electrophoresis is an important molecular biology tool which enables us to study DNA. It can be used to determine the sequence of [more...]
Date: 2010-09-08 04:07:38
Genetic alterations common to Kabuki syndrome discovered through ...
Second-generation sequencing technologies have some difficulty identifying some types of genetic variants that lead to a loss of function in genes. Follow-up sequencing using the traditional Sanger DNA sequencing method, [more...]
Date: 2010-08-15 18:15:00
Research Demonstrates Progress Towards DNA Strand Sequencing
UCSC researchers are collaborating with Oxford Nanopore Technologies Ltd in the development of a new generation of electronic, single-molecule DNA sequencing technology. In the 'strand sequencing' method, current through a nanopore is [more...]
Date: 2010-10-02 15:03:00
SpringerProtocols: Abstract: The State-of-the-Art of Chromatin ...
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the DNA sequences associated with it. ChIP has been widely used to map the localization of post-translationally modified histones, histone variants, transcription factors, or chromatin-modifying enzymes on the genome or on a given locus. In [more...]
Date: 2009-08-01 04:00:00
E. Coli Plasmid Vectors: Methods and Applications (Methods in ...
E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) (archive) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, N.J.), V. 235.) Table of Contents The Function and Organization of Plasmids Finbarr Hayes Choosing a Cloning Vector Andrew Preston Escherichia coli Host Strains Nicola Casali Chemical Transformation of E. coli W. Edward Swords Electroporation of E. coli Claire A. Woodall DNA Transfer by Bacterial Conjugation [more...]
Date: 2004-04-04 08:00:00
DNA Sequencing: New directions, and potential implications to ...
DNA Sequencing: New directions, and potential implications to healthcare Wired News (blog) However, much lower cost and more efficient methods for DNA sequencing will be required to make it feasible for routine healthcare practice and [more...]
Date: 2010-10-07 21:34:01
Synthetic Biology: Building Life (Potential & Dangers ...
But today, applications of our understanding of DNA in areas such as genetic engineering and cloning seem primitive compared to synthetic biology, a field in which the basic building blocks of life are pieced together from scratch to suit the needs of research, medicine, and the environment. Synthetic biology combines knowledge of genetics and cell biology with principles of engineering and is limited only by scientistsâ imaginations.Â But the field is still in its infancy, and initial ventures into figuring out how best to put the basic components of DNA together synthetically have [more...]
Date: 2009-03-10 17:15:19
Biotechnology, XII: Model Paper « Home works and Assignments Online
Which vectors are used in human genome sequencing & why? Explain the blue white method of screening the recombinant plasmid? A single cell of E.coli is supposed to produce about 2000 different proteins. under optimum conditions. One of the desired intercellular enzymes produced is in the form of 2500 molecules/cells. If the molecular weight of that enzyme is 100000D.How many E.coli cells are required to produce 1gm of enzymes. What are secondary metabolites? Name the secondary metabolites produced by the following plants. (a)Atropa beladona (b) Cinchona officinalis. SECTION C (3 marks [more...]
Date: 2010-10-13 05:07:00
Bioinformatics and its applications
Shotgun sequence techniques are also used for sequence analysis of numerous fragments of DNA. Special software is used to see the overlapping of fragments and their assembly. Prediction of Protein Structure:- It is easy to determine the primary structure of proteins in the form of amino acids which are present on the DNA molecule but it is difficult to determine the secondary, tertiary or quaternary structures of proteins. For this purpose either the method of crystallography is used or tools of bioinformatics can also be used to determine the complex protein structures. Genome [more...]
Date: 2010-09-24 07:00:00
Epigenetics Papers: A Genomic Sequencing Protocol that Yields a ...
Genomic sequencing protocols, which have been developed to ascertain the methylation status of selected regions within genes, utilize the Maxam and Gilbert chemical cleavage reactions [. ... To address these problems, we have developed a genomic sequencing method that provides positive identification and localization of 5-methylcytosine in genomic DNA. The method is based on sodium bisulfite-mediated conversion of cytosine to uracil in single-stranded DNA, followed by PCR [more...]
Date: 2007-03-13 21:09:00
Scientist Solutions - The Sanger DNA Sequencing Method
Would you like to save this topic, event, protocol or job so you can find it again easily? Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space. Available to members only. Please log in or register for your free account [more...]
Date: 2009-02-04 18:51:55
Venture Development Center NobleGen Biosciences joins VDC
With a proof of concept in hand, startup NobleGen Biosciences is setting up at the VDC to develop and commercialize a single-molecule sequencing technology that uses arrays of solid-state nanopores and optical detection. The method comes from the lab of Amit Meller at BU and colleagues at the University of Massachusetts Medical School. Meller filed his first patents on the technology while working at Harvard. NobleGen recently licensed a patent portfolio from Harvard and BU that includes additional IP from Meller’s research at BU. The company is led by CEO Frank Feist and Phil Buzby, former [more...]
Date: 2010-10-06 12:41:09
Halfbakery: Ligase sequencing
Currently the standard method (Sanger sequencing, or di-deoxy sequencing, or dye-terminator sequencing) is to purify the DNA, then transcribe a section from one particular point in the presence of fluoresently labelled chain terminators [more...]
Date: 2005-08-05 00:00:00
Allele-specific DNA methylation: beyond imprinting — Hum Mol Genet
Allele-specific DNA methylation (ASM) and allele-specific gene expression (ASE) have long been studied in genomic imprinting and X chromosome inactivation. But these types of allelic asymmetries, along with allele-specific transcription factor binding (ASTF), have turned out to be far more pervasive—affecting many non-imprinted autosomal genes in normal human tissues. ASM, ASE and ASTF have now been mapped genome-wide by microarray-based methods and NextGen sequencing. Multiple studies agree that all three types of allelic asymmetries, as well as the related phenomena of expression and [more...]
Date: 2010-09-21 13:17:53
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
JoVE: Electroeluting DNA Fragments (Video Protocol)
Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution from glass or silica particles, DEAE-cellulose membranes, "crush and soak method", electroelution or very often expensive commercial purification kits. Thus, selecting a method will depend mostly of what is available in the laboratory. The [more...]
Date: 2010-09-05 22:00:00
dna testing kit cvs | Paternity Tests
dna testing kit cvs. December 31, 2009. How do you define DNA testing? It is specified testing that looks for the absence or presence of DNA sequences. It makes use of molecular methods like DNA chips, arrays, or polymerase chain [more...]
Date: 2009-12-31 11:13:07
Leading Edge DNA Sequencing Method Nets Major NIH
Described in the May 12 online edition of Nano Letters, their novel, highly efficient, optically-based method to detect single DNA molecules in nanopores could significantly reduce the cost of DNA sequencing and the time required to sequence a complete human genome. “We are the first to employ optical detection from individual nanopores, and this allows us to probe multiple pores simultaneously using a single high-speed CCD camera,” said Meller, referring to the charge-coupled devices that science and medical researchers use to obtain high quality images. “As a result, our method can [more...]
Date: 2010-09-13 04:00:00
Journal of Clinical Investigation -- Human C3 mutation reveals a ...
The chromatogram corresponding to the DNA sequence surrounding the mutated nucleotides in C3 is shown for GN28 and a control sample. The corresponding amino acid sequences for the WT and mutated alleles are indicated. Amino acid numbering refers to the translation start site (Met +1), and the nucleotide nomenclature refers to nucleotide A in the ATG translation initiation codon, according to Human Genome Variation Society recommendations for description of sequence variants. The C3923ΔDG mutation deletes 2 amino acids within MG7 in the polypeptide linking the MG7 and CUB domains. The [more...]
Date: 2010-09-12 07:00:00
see the world from science lens: Nature Biotechnology Contents ...
Genetic testing for rare heart conditions might someday expand to more common cardiac ailments. Already there are signs testing is dramatically changing how some conditions are treated and doctors' definition of who a patient is. .... Methods for profiling DNA methylation differ in the physical principles used to detect modified cytosines. Harris et al. compare the performances of four sequencing-based technologies for genome-wide analysis of DNA methylation and combine [more...]
Date: 2010-10-13 19:53:00
How Genomic Research and Antibody Catalogues Work Together | Novus ...
DNA sequencing, the method of determining the order of the nucleotide bases (adenine, guanine, cytosine, and thymine) within the DNA molecule, is at the forefront of antibody research. Once we know the sequence of nucleotides, [more...]
Date: 2010-09-22 10:32:28
Gene Cloning and DNA Analysis: An Introduction (Brown,Gene Cloning ...
Explaining the significance, the principles, the techniques, and the applications of gene cloning and DNA analysis, this introduction assumes little prior knowledge on the part of the reader. Particular attention is given to the methods of sequencing genomes and analyzing this sequence. The applications to forensics science are highlighted. Approximately 250 color illustrations are featured. Brown teaches biomolecular archeology at [more...]
Date: 2010-07-22 21:11:10
DNA sequencing - the 454 method
Watch the Wellcome Trust's animation about 454 machines, which are able to read one gigabase of DNA sequence in a couple of days, at a cost of $0.02 per 1000 [more...]
Date: 2010-05-21 13:28:22
Improving Molecular Detection of Fungal DNA in Formalin-Fixed ...
assays, followed by sequencing of the DNA fragments ob- tained. The protocols were assessed for time spent in perform- ing the procedure, quality of DNA detection, and efficiency of fungal-DNA detection. MATERIALS AND METHODS [more...]
Date: 2010-08-27 20:37:26
AIR™ DNA Sample Prep Kits Now Available for Illumina-Compatible ...
Bioo Scientific has expanded its line of kits for next generation sequencing sample preparation with the introduction of the AIR Genomic and AIR Paired-End DNA Sequencing Kits, designed for use with the llumina GAII and HiSeq 2000 sequencers. These kits offer flexible, cost-effective, single day sample preparation protocols. It has become obvious that the use of the same preparatory methods to identify and quantify rare sequences and perform large scale comparative evolutionary studies is not ideal and that there are clear advantages for particular applications over others. Bioo [more...]
Date: 2010-10-05 18:12:01
RNA-Seq: Comparison of sequencing-based methods to profile DNA ...
The four most frequently used sequencing-based technologies are the bisulfite-based methods MethylC-seq and reduced representation bisulfite sequencing (RRBS), and the enrichment-based techniques methylated DNA immunoprecipitation sequencing (MeDIP-seq) and methylated DNA binding domain sequencing (MBD-seq). We applied all four methods to biological replicates of human embryonic stem cells to assess their genome-wide CpG coverage, resolution, cost, concordance and the influence of CpG density and genomic context. The methylation levels assessed by the two bisulfite methods were concordant [more...]
Date: 2010-09-21 10:00:06
Effect of N3-Methyladenine and an Isosteric Stable Analogue on DNA ...
A. M. Maxam and W. Gilbert, “Sequencing end-labeled DNA with base-specific chemical cleavages,” Methods in Enzymology, vol. 65, no. 1, pp. 499–560, 1980. M. L. Kopka, C. Yoon, D. Goodsell, P. Pjura, and R. E. Dickerson, “The molecular [more...]
Date: 2010-06-25 07:00:00
My Weblog on Bioinformatics, Genome Science, Next Generation ...
Just saw this paper Sequencing and analysis of an Irish human genome. AFAIK WGS is usually done at 30x coverage. In this paper, the authors “describe a novel method for improving SNP calling accuracy at low genome coverage using haplotype information.” I thought it was pretty good considering that they had 99.3% of the reference genome covered for 10.6x coverage. That leaves only like 21 Mbases missing .. For those interested in the tech details Four single-end and five paired-end DNA libraries were generated and sequenced using a GAII Illumina Genome Analyzer. The read lengths of the [more...]
Date: 2010-10-12 10:49:00
FinchTalk: From Reads to Data Sets, Why Next Gen is not like ...
The first widely-used sequencing systems were based on the “Sanger” method. DNA was synthesized in the presence of chain terminating radioactive dideoxy-nucleotides (1). Mixtures of DNA fragments were separated by size using gel electrophoresis and the bases were identified and entered into a computer through manual techniques. Automated DNA sequencing instruments arrived later. These instruments made DNA sequencing thousands of times more efficient by detecting fluorescently labeled fragments and sending the information directly to a computer (2). For the first time, it became possible [more...]
Date: 2009-01-06 19:16:00
massively parallel DNA sequencing Systematic comparison of three ...
with high-throughput electrophoresis-based sequencing methods. (Margulies et al. 2005; Shendure et al. 2005; Bentley et al. 2008). Although advances in these new technologies are being made at a rapid pace, the cost of sequencing a [more...]
Date: 2010-09-01 17:56:09
New DNA Diagnostic Test For Lyme Disease May Have Merit
Milford Hospital in Massachusetts has been working for over a year on a new type of diagnostic test for Lyme disease using DNA sequencing rather than DNA nesting. Huh? We have all heard of the polymerase chain reaction (PCR) method of [more...]
Date: 2010-09-17 03:38:41