Ovarian Cancer Diagnostics | Ovaire
DNA testing reagents for cancer CTC Test markets Biochip Innovations New companies with new approaches "Laggards" Companies that have not met expectations Protein important molecular markers used in tissue staining Biomarkers under investigation Mergers and Acquisitions Status of pharmacogenomic tests Microarrays Markets in clinical laboratories The lists of suppliers and main products Unmet market needs This report includes market analysis of the following tests: The Profile Cancer CTC, fecal occult blood, PSA, H. pylori, IHC, ISH, and evidence of specific cancers: Bladder [more...]
Date: 2010-10-11 18:38:23
Company Profile : Diagnostic supplier : Suyog Diagnostics : Mumbai ...
The company is representing world renowned Diagnostics Companies and selling Diagnostics Kits for Coagulation disorders, Viral Markers for HIV, HBV, HCV, HDV, HEV, Biochemistry, Chromatography kits etc. Life Science Division: The company is representing world renowned Diagnostics Companies and selling Chemicals, Bio - Chemicals, FISH Probes, Radio Chemicals, Reagents, Cell Bio, Electrophoresis grade, Neuro chemicals, Molecular Biology grade, DNA Extraction kits, PCR Kits, Restriction Enzymes, DNA Ladders & modifying enzymes, Proteomics, Cell Culture reagents, Plant Tissue Culture Grade [more...]
Date: 2010-07-09 15:29:00
SciWorks to Host BioTechnology Days 9/23/10 and 9/24/10
Winston-Salem, NC: The future of the Piedmont Triad resides in science education and the growth of the biotech industry. As part of the NC Science Festival and in response to the increased need for science education, SciWorks Science Center is partnering with the NC BioNetwork and Connect a Million Minds, an initiative of Time Warner Cable, to provide hands-on activities that will show how biotechnology is part of our every day lives. Also participating is the Center for Excellence in Research, Teaching and Learning (CERTL) of Wake Forest University Baptist Medical Center. Middle School [more...]
Date: 2010-09-21 12:00:00
ICTS Pilot Grant Recipients Forge New Ground to Identify Disease ...
DNA is a duplex, therefore the patient's DNA, labeled with a dye, will hybridize to DNA on the array. The investigators then treat the array with an enzyme called a mismatch endonuclease that will seek out and digest any places in the DNA duplexes that contain mismatches, thus identifying the mutation's location. “You take someone’s genomic DNA, chop it up, label it using a florescent dye, and hybridize it to the microarray so that complementary features on the array can bind with the labeled DNA,” explained Manak. “Treatment with the enzyme results in cleavage at the mismatches, [more...]
Date: 2010-07-22 21:40:50
Plasmid DNA extraction from e.coli for digestion mapping clony ...
Research news, bioproducts, protocols, reagents, forums Plasmid DNA extraction from e.coli for digestion mapping clony screening Plasmid DNA extraction from e.coli for digestion mapping clony screening: This protocol utilize the Qiagen buffer P1, P2 and P3 from Qiagen plasmid purification kit. RNase must be added before use. It is cost efficient since it doesn't use expensive plasmid purification columns. This protocol is only intended for screening positive clony. Once positive clonies are determined, purification from bacterial overnight culture can be done using plasmid [more...]
Date: 2006-08-30 07:00:00
A robust universal method for extraction of genomic DNA from ...
The intactness of DNA is the keystone of genome-based clinical investigations, where rapid molecular detection of life-threatening bacteria is largely dependent on the isolation of high-quality DNA. Various protocols have been so far developed for genomic DNA isolation from bacteria, most of which have been claimed to be reproducible with relatively good yields of high-quality DNA. Nonetheless, they are not fully applicable to various types of bacteria, their processing cost is relatively high, and some toxic reagents are used. The routine protocols for DNA extraction appear to be [more...]
Date: 2010-08-21 07:44:00
Evaluation of Five Methods for Total DNA Extraction from Western ...
DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. Methodology/Principal Findings From individual western corn rootworm beetles, Diabrotica virgifera virgifera, DNA extractions by the SDS method, CTAB method, DNAzol® reagent, Puregene® solutions and DNeasy® column were compared in terms of DNA quantity and quality, cost of materials, [more...]
Date: 2010-08-13 07:00:00
Standardized Comet Assay System For Detection of DNA Damage ...
The AMSBIO range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signalling, DNA damage, electrophoresis, glycobiology, post translational modification and stem cell biology. Worldwide HQ AMS Biotechnology (AMSBIO) 184 Milton Park Abingdon Oxon OX14 4SE UK Tel: +44 1235-828200 Fax: +44 1235-820482 Email: firstname.lastname@example.org Web: [more...]
Date: 2010-09-18 07:00:00
DNA analysis – Using reagents?
The only problem is that when the cell's DNA is released from its protective nuclear membrane during extraction, the cell's DNA becomes exposed to its very own DNases. Some extraction protocols call for the use of special reagents that [more...]
Date: 2010-08-10 04:18:09
DNA Science: A First Course, Second Edition
It contains a fully upÂ–toÂ–date collection of 12 rigorously tested and reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule. Proven through more than 10 yearsÂ’ of teaching at research and nonresearch colleges and universities, junior colleges, community colleges, and advanced biology programs in high school, this book has been successfully integrated into introductory biology, general biology, genetics, [more...]
Date: 2010-05-13 09:15:50
Technical Writer – Bioinformatics
CT-Branford, About Roche 454 Life Sciences: Roche 454 Life Sciences develops and commercializes instrumentation, reagents, and services for high-throughput DNA sequencing. Simple, unbiased sample preparation and massively parallel [more...]
Date: 2010-10-10 16:01:57
DNA strides into organic synthesis
The walker and three 'stations', made from specific DNA sequences, organise themselves along the track using the normal pairing of DNA bases. 'The walker will move unidirectionally along the track,' Liu says. 'We attached chemical reactants to the walker and each station on the track. As the DNA walker moves from station to station, a programmed series of chemical reactions takes place.' The walking strand of DNA moves to each station (blue, red and green) in turn and reacts with the attached reagent © Nature Nanotechnol. In this way, the Harvard team was able to perform three acylation [more...]
Date: 2010-10-10 22:46:00
FTDNA Family Finder test
(BUSINESS WIRE)--Family Tree DNA, the first and largest genealogical DNA testing company, today announced the launch of the Family Finder DNA test for connecting family members across all ancestral lines. The test utilizes Affymetrix’ recently launched Axiom™ genotyping technology and the GeneTitan® System to confidently match a wide range of family relationships within five generations. Family Finder represents a major advancement over earlier genealogical tests, which revealed only connections along specific paternal lines (for males) or maternal lines (males or females). [more...]
Date: 2010-05-15 07:13:00
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
DNA Isolation and Modeling « NWABR
This entertaining and inexpensive protocol was adapted from Biotechnology in the Classroom, UC Davis and Diane Sweeney, Biology — Exploring Life. First we, the teacher-students line up for our materials: Dr. Hutchison regales us as we select strawberries and collect materials: This flexible protocol has been used with first graders all the way through high school and administrators. You can make it as in depth as you like. All materials can be bought in a grocery store, there are no expensive reagents. This was my lovely strawberry, in a sealed plastic bag and on a paper towel, [more...]
Date: 2010-03-07 00:00:57
Research Associate (San Diego) | PCR Jobs
"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:Â Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.Â Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.Â Prepares technical reports, summaries, protocols and quantitative analyses.Â Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]
Date: 2010-10-01 19:07:24
solutions manual to introduction to particle technology ...
High-Throughput Solutions for DNA Purification Using MagneSil … INTRODUCTION. MagneSilâ„¢ technology was developed specifically to meet the changing … small particle size allows rapid movement in a magnetic field, resulting … plasmid DNA in <2 hours with no manual interventions. Unique magnetic … http://www.promega.com/pnotes/75/8554_07/8554_07.pdf * pdf N07848_ver2.5 MagMAX Express User Manual Principle of the MagMAXâ„¢ Express magnetic particle processor . ….. Chapter 3: Introduction to the MagMAXâ„¢ Express …. The operating principle employed is MPP (inverse [more...]
Date: 2010-10-13 07:08:10
Comments: Life Technologies buys Ion Torrent « Omically Speaking
There was some exciting news yesterday from the next-generation sequencing (NGS) field, with the buy-out of Ion Torrent - a start-up that’s developed a novel DNA sequencing machine – by the life-sciences behemoth Life Technologies (for a whopping 350million dollars). The technology for sequencing of DNA – the blueprint of all life – is at an exciting stage. Ten years ago, the complete human genome was sequenced at the cost of several billion dollars and decades of attempt. Today, a full genome can be sequenced for as little as $10,000 and in less than a week. The price and time are [more...]
Date: 2010-08-18 17:13:21
Technology Review: A Portable DNA Detector
A new portable DNA analyzer performs real-time analysis of blood samples left at the scene of a crime. Researchers at the University of California, Berkeley, developed the device, which packs microfluidics, electronics, optics, and chemical detection technology into a single briefcase-sized unit. "While previous groups have developed lab-on-a-chip systems, none of them have succeeded in making a completely portable, robust system that can be used at a scene," says team leader Richard Mathies. On-site CSI: After a blood sample is collected from the scene of a crime, this briefcase-sized [more...]
Date: 2008-09-24 04:00:00
Plasmid DNA Recovery Using Size-Exclusion and Perfusion ...
Finally, a second size-exclusion chromatography step was carried out to purify the plasmid DNA from other small molecular-weight contaminants. Analytical methods proved that the purified plasmid DNA had a purity of 95% after Sephacryl S1000. Plasmid identity was confirmed by restriction enzyme digestion. Biological activity of the purified plasmid was confirmed in vivo; immunized mice developed a positive antibody response against all HCV structural antigens. This procedure offers an alternative to traditional methods that use organic reagents, mutagenic and toxic compounds, and [more...]
Date: 2008-09-01 04:00:00
The Purification of Plasmid DNA for Clinical Trials Using Membrane ...
ABSTRACTMembrane chromatography offers a good solution to the challenge of developing an efficient chromatographic procedure for plasmid DNA purification. The large convective pores of anion exchange membranes allow plasmid DNA to access all the anionic binding sites of the membrane at high flow rates. Here we demonstrate that the pIDKE2 plasmid can be purified from a recombinant Escherichia coli lysate using a Sartobind D membrane combined with size exclusion chromatography to render material with 95% purity and an average yield of 50%. This process yields therapeutically suitable plasmid [more...]
Date: 2010-02-01 05:00:00
New Device Will Slash Time for DNA Analysis | UANews.org
"Our DNA analysis platform is a fully automated system that doesn't require any human intervention," Zenhausern said. "You load the specimen in a cartridge that can contain all the necessary reagents for processing a sample and place it in the instrument, close the instrument and push a button on the computer, which will control all processing and analysis steps for generating data that can then be loaded in a DNA expert software system for identifying a human profile and matching an offender. That's it." The center developed the technology for the device and designed it for use by law [more...]
Date: 2010-08-17 07:00:00
Biotinylation of Antibodies | Biotinylated and Biotinylation
Biotin is a material generally used in a double step recognition systems inrecital with conjugated avidin.It is naturally conjugated to proteins via primary amines for example lysines. Typically between three to six biotin molecules are conjugated to an antibody. There are other measures used by this code of behaviour such as 1. Column chromatography 2. Reagent Storage The conjugation protocol consists of, 1. Preparation of antibody 2. Covalent conjugation Conjugation protocol: The complete conjugation process can be performed in a single day. You will need the resources named below. [more...]
Date: 2010-01-13 00:47:14
FamilyTreeDNA.com: New Family Finder test officially launched
Tracing the Tribe is a blog about Jewish genealogy - All the developments, tools and resources you'll need to peer more closely into your family tree. Created in 2006 at JTA's request, it is now independent. As of today, FamilyTreeDNA.com's new Family Finder test has been officially launched. The new test connects family members across all ancestral lines, not only paternal or maternal. It represents a major advancement over earlier genetic genealogy tests. Everyone, regardless of gender, can now look for connections including grandparents, aunts and uncles, half siblings, and first, [more...]
Date: 2010-05-03 17:15:00
Links Between XMRV and Human Disease Studied | Health News
A previous study in the United States found evidence of XMRV DNA in 67 percent of subjects with chronic fatigue syndrome (CFS), compared to 3.7 percent of healthy controls. Subsequent studies in Europe, however, were unable to demonstrate the presence of XMRV in CFS subjects or healthy controls. “The findings suggest that in the population we tested, XMRV is not associated with chronic fatigue syndrome and suggests that differences in PCR technique from study to study do not explain the disparate results seen in XMRV studies of chronic fatigue syndrome,” Dr. Tsibris said. A second study, [more...]
Date: 2010-10-13 06:57:52
Ultrafast EMCCD cameras point the way to faster DNA sequencing
To harness this powerful single-molecule detection modality for high-throughput DNA sequencing, each of the four nucleotides (A, C, G and T) in the target DNA has to be converted into a predefined sequence of oligonucleotides, using a technique called Circular DNA Conversion. Each pre-defined sequence is then hybridized to a self-quenching molecular beacon.In their experimental setup, the Meller team recorded DNA translocation through a 4nm pore in a SiN membrane immersed between two aqueous solutions. With the camera in place, the researchers were able to detect florescence bursts in two [more...]
Date: 2010-06-10 07:00:00
BIO-SYNTHESIS - Custom Antibody Custom Peptide Synthesis Custom ...
BIO-SYNTHESIS, INC. is a leading life science products company with over 20 years of experience in the design and synthesis of Custom Peptide, small molecules and reagents for small scale research and bulk pharmaceutical trials. Using state of the art technology in our well-equipped laboratories. Emerin Antibody Catalog# : 4031 Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family that includes proteins such as LAP2 and MAN1. Each family member, including Emerin, has an ~40 amino acid LEM-domains that binds barrier-to-autointegration [more...]
Date: 2010-10-13 10:34:00
New - Used - Reconditioned and Refurbished Laboratory Equipments ...
Autoclaves Balances and Scales Blood Gas Analyzers Blood Gas Reagents, Controls, Consumables Coagulation Analyzers Co-Oximeters Colposcopes Differential Counters Densitometers Dry Bath Incubators Electrolyte Analyzers Electrolyte Reagents, Controls, Consumables Flow Cytometers Gamma Counters Hematology Analyzers Hematology Reagents, Controls, Consumables Histology Microplate readers and washers are used for a variety of laboratory purposes. Microplate readers are designed to scan, analyze and obtain numerical results from chemical reactions conducted in microplates, whereas microplate washers [more...]
Date: 2010-10-13 05:23:00
Efficient light collection and high frame rates a must for high ...
To harness this powerful single-molecule detection modality for high-throughput DNA sequencing, each of the four nucleotides (A, C, G and T) in the target DNA has to be converted into a predefined sequence of oligonucleotides, using a technique called Circular DNA Conversion. Each pre-defined sequence is then hybridized to a self-quenching molecular beacon. In their experimental setup, the Meller team recorded DNA translocation through a 4 nm pore in a SiN membrane immersed between two aqueous solutions. With the camera in place, the researchers were able to detect fluorescence bursts in two [more...]
Date: 2010-06-15 11:25:33
Antigen/Antibody Discovery Research Scientist (Sorrento Mesa, San ...
"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! CTK Biotech Inc, a leading IVD biotech company located in San Diego area, is seeking an Antigen/Antibody Discovery Research Scientist to join a group of scientists and technicians in the R&D Department. The current position will play a major role in researching and discovering high quality and specific diagnostic reagents to meet the rapidly growing demand of the point-of-care IVD products worldwide. Essential Duties and Responsibilities: Note: Other duties may be assigned. Perform cloning and [more...]
Date: 2010-10-12 03:53:43
تقنية البلمرة الجزيئية » تعرف عن عملية استخلاص شريط الحمض النووي ...
The VX Viral DNA/RNA Purification CorProtocol™ 25101 allows for walk-away automated preparation of low copy viral DNA/RNA from low total nucleic acid-yielding samples. Extracted viral DNA/RNA is of high quality and suitable for a wide variety of downstream applications. The default sample volume is 200 µL but can be scaled up to 500 µL with the use of adjustments to this protocol coupled with an alternative run file ( VX_RNA_DNA_CorProtocol_250_500ul_25101_06.CAS4 ) on the instrument. Increased volumes of certain reagents are required for the extraction of sample volumes greater [more...]
Date: 2010-07-09 16:20:26
DNA microarrays to monitor the health of farmed fish
The antibody microarray is the arrangement of antibodies on a small plate of glass, each antibody being able to recognize and bind specifically to a protein in the blood during an infection . When an antibody has bound to a protein present in the blood sample , a reagent fluoresces red or green . This allows to visualize the presence and extent of infection. As for the protein chip , it is a piece of litmus paper on which are disposed of proteins characteristic of several diseases that may affect the flounder ( eg Edwardsiellose ) . If the fish is or has been affected by a disease, it has [more...]
Date: 2010-09-06 15:24:16
Free Online Guides: Anti-tumor drugs tested by microfluidic device
With some other in vitro tests, like DNA analysis, cells need to be killed in order to be studied. In this analysis method, a change in fluorescence occurs when caspase-3, an indicator of cell apoptosis, is activated. In Wu's test, increasing concentrations of etoposide demonstrated correspondingly higher activation of caspase-3. In a control-group chip without etoposide, caspase-3 was not activated.Wu also said that, "Unlike conventional methods, the microfluidic device permits quantitative data to be obtained from individual cells; the device requires fewer numbers of cells and allows [more...]
Date: 2010-10-10 20:09:00
Plant RNA/DNA Purification Kit
Total DNA and RNA from 50 mg of tobacco, tomato and peach were simultaneously isolated with Bio-Synthesis's Plant RNA/DNA Purification Kit (with the optional on-column RNase treatment). Next, 2µL of DNA from each 150 µL elution was mixed in 20 µL of PCR reaction and used in a real-time PCR (95ºC for 3min and 40 cycles at 95 ºC for 15 sec. and 60 ºC for 30 sec.). DNA from tobacco (green), tomato (red) and peach (blue) was successfully amplified, indicating the high quality of the DNA for downstream application. Primer dimer (black) also was shown. Figure 3. Isolate High Quality Total RNA [more...]
Date: 2010-08-16 04:44:00
Science: DNA Mutation in c-Myb Gene linked to Leukaemia | The …
Here we demonstrate that chemical mutagenesis of mice combined with advances in hematopoietic stem cell reagents and genome resources can efficiently recover recessive mutations and identify genes essential for generation and … Go here to see the original: Science: DNA Mutation in c-Myb Gene linked to Leukaemia | The … This entry was posted on Tuesday, August 10th, 2010 at 2:49 pm and is filed under Stem Cell Advances. You can follow any responses to this entry through the RSS 2.0 feed. Both comments and pings are currently [more...]
Date: 2010-08-10 14:49:17
Cytoplasmic & Nuclear RNA Purification Kit
RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]
Date: 2010-08-16 04:58:00
Suggested Procedures for the Detection and Identification of ...
6.2 PCR – Quality Assurance/Quality Control 6.3 PCR Protocols 6.4 Reagents 6.5 Analysis of Extracted DNA Using an UV Spectrophotometer 6.6 Table of PCR Protocols 6.7 References Appendix 1 The Handbook and Oversight Committee A1.1 Format of the Handbook A1.2 Handbook Revision and Oversight Committee A1.3 Process for Amending the Handbook Appendix 2 Handbook Committee Members A2.1 2000 – 2002 Handbook Development Committee A2.2 2002 – 2003 Handbook Development Committee A2.3 2003 – 2004 Handbook Development Committee A2.4 2004 – 2005 Handbook Development Committee A2.5 2006 – 2007 [more...]
Date: 2010-10-06 19:55:54
T4 Polynucleotide Kinase 500 u EK0031 450,000 T4 DNA Ligase (With PEG) 200 u EL0335 450,000 T4 DNA Ligase 200 u EL0015 450,000 T4 DNA Polymerase 100 u EP0061 440,000 Taq DNA Polymerase (native) 100 u EP0281 470,000 Taq DNA Polymerase (native) 500 u EP0282 1,880,000 Taq DNA Polymerase (recombinant) 100 u TA7505C 50,000 Taq DNA Polymerase (recombinant) 5,000 TA7507C 1.200,000 Taq DNA Polymerase (recombinant) 500 u TA7506C 160,000 TaqI 3,000 u RE7717C 230,000 TBE Buffer, 5X 50 ml MR7725C 20,000 Total Protein (Automatic and manual) 300Tests BK8145C 25,000 Triglycerides (Automatic and manual) New [more...]
Date: 2005-10-25 10:14:00
Genomic DNA extraction protocol protocols and methods
Research news, bioproducts, protocols, reagents, forums Genomic DNA extraction protocol High molecular weight genomic DNA extraction protocol: Buffer and reagent: Genomic DNA extraction buffer (250ml): 1M Tris.Cl (pH 8.0) 2.5ml 0.5M EDTA (pH 8.0) 50 ml Pancreatic RNase 5 mg 10% SDS 12.5 ml -------------------------------------------------- Adjust pH to 8.0 and adjust volume to 250ml with ddH2O Saturated phenol (pH 8.0) 10M ammonium acetate (NH4Ac) Protocol: 1. Weigh 0.5-1g fresh tissue and put in motar. Add liquid nitrogen to snap freeze tissue and blend tissue to [more...]
Date: 2006-08-29 00:00:00
WCU installs new DNA sequencing equipment | The Reporter
The studies that forensic science faculty members are planning for Western Carolina University’s new next-generation DNA sequencer could help introduce the technology into crime laboratory casework. Brittania Bintz holds a pico-titer plate containing DNA and reagents for sequencing. Western Carolina University forensic science faculty recently completed installation of a new next-generation DNA sequencer called a GS Junior System. WCU acquired and recently completed installation of a GS Junior System, an instrument that Roche Diagnostic Corp. launched globally in May. The instrument [more...]
Date: 2010-09-14 21:02:20
automated dna sequencing
Fluorescence-based DNA sequencing systems use dideoxy chain A text file of the inferred DNA sequence is output directly. Automated DNA Sequencing Guidelines: One of the most common problems encountered in automated DNA sequencing DNA sequences will be available in 2-4 days after they are submitted. Automated DNA Sequencing Request Automated DNA Sequencing Request. Date: Requested By: Principal Investigator: P.O. DNA SEQUENCING. – See website for volume discount on BigDye XTerminator sequencing . Automated DNA Sequencing – Biocompare Automated DNA Sequencing DNA Sequencing | [more...]
Date: 2010-07-09 06:51:50
genomic DNA PCR protocols and methods
Research news, bioproducts, protocols, reagents, forums genomic DNA PCR > Can someone forward me a general protocol for doing PCR using genomic > DNA as the template? I seem to recall needing about 100 ng of DNA, but > I forget stuff like how much Taq to use, concentration of dNTP's, and > concentration of primers. General protocol for PCR Each person uses a slightly different ratio of reagents. For DNA amplicons up to 500 bp, use 25 uL reaction volumes as follows: On ice add to thin-walled pcr tubes 0.125 uL Taq polymerase (1.25 U, Gibco) 2.5 uL of 10 x PCR buffer minus Mg [more...]
Date: 2004-12-27 00:00:00