Our blogs and videos choice: dna quantification

Methylation Test May Aid Colon Cancer Diagnosis (CME/CE) | Discuss ...

To develop a more sensitive test for DNA methylation, investigators employed a form of technology known as BEAMing (beads, emulsion, amplification, and magnetics). They performed PCR amplification of individual DNA fragments covalently linked to magnetic beads suspended in water-based nanoparticles immersed in oil droplets. The beads contained a DNA sequence specific for exon 1 of the vimentin gene, which is hypermethylated in colon cancer. Following PCR amplification, the beads were hybridized by means of fluorescent probes specific to the state of methylation and then sorted and analyzed by [more...]

Date: 2010-10-09 11:00:27

Blog posts (13) | Videos (9)
 


Whole blood and tissue fungal DNA quantification in the diagnosis ...

3.2. Fungal DNA quantification. Fungal DNA was detected by the PenAsp assay in all nasal biopsy samples except in the samples from two dogs with nasal neoplasia (Fig. 1). Although the relative copy numbers overlapped between the four [more...]

Date: 2008-10-01 07:00:00


Team:Newcastle/IntroductoryLabwork/16 July 2009

We chose to follow the Spin-method and carried out steps 1-6 and 1b to 11b missing out the DNA concentration step and also missing out DNA quantification. This is a summary of the [more...]

Date: 2010-08-20 11:58:38


INDONESIAN ENTERPRISE INDONESIAN ENTERPRISE

Very good for DNA quantification applications. Spesifikasi : Wavelength range: 190 – 1, 100 nm Measure time for full scan range: Less than 5 seconds Wavelength reproducibility : < ± 0.2 nm Wavelength accuracy: ± 2 nm [more...]

Date: 2010-10-02 23:14:24


IJERPH | Free Full-Text | Different Length (DL) qPCR for ...

Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage Knut Rudi 1,2,*,* , Irina Hagen 1 , Bente Carina Johnsrud 1 , Guro Skjefstad 1 and Ingun Tryland 3 1 Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway 2 NOFIMA Mat, Ås, Norway 3 NIVA Norwegian Water Research Institute, Oslo, [more...]

Date: 2010-08-31 07:00:00


Science Literature - "Truth be told" about Chimp-Human DNA comparisons

"Truth be told" about Chimp-Human DNA comparisons 07/02/07 For over 30 years, the public have been led to believe that human and chimpanzee genetics differ by mere 1%. This 'fact' of science has been used on innumerable occasions to silence anyone who offered the thought that humans are special among the animal kingdom. "Today we take as a given that the two species are genetically 99% the same." However, this "given" is about to be discarded. Apparently, it is now OK to openly acknowledge that those who are involved in this research have never been comfortable that the 1% figure was an [more...]

Date: 2007-07-02 12:37:52


Trinean launches DropSense96 and DropPlate 16 G for genomic DNA ...

<a href="http://enterprisepost.com/biomed/2010/10/11" title="Weight loss pill off shelves due to heart attack risk, “Black Death” of the Middle ages blamed on bacteria, Some herbal remedies for anxiety disorders effective, Bathing better than pethidine as pain relief during labor: Survey, Meridia – weight loss pill off the shelves in US, Study finds monarch butterflies use medicinal plants to treat offspring for disease, UT Southwestern study to determine whether leptin helps type 1 diabetes patients, When in Rome: Study-abroad students increase alcohol intake, Insurance and [more...]

Date: 2010-04-24 08:32:17


Trinean Reduces Volume and Time Requirements for Genomic DNA ...

<a href="http://enterprisepost.com/biomed/2010/10/11" title="Weight loss pill off shelves due to heart attack risk, “Black Death” of the Middle ages blamed on bacteria, Some herbal remedies for anxiety disorders effective, Bathing better than pethidine as pain relief during labor: Survey, Meridia – weight loss pill off the shelves in US, Study finds monarch butterflies use medicinal plants to treat offspring for disease, UT Southwestern study to determine whether leptin helps type 1 diabetes patients, When in Rome: Study-abroad students increase alcohol intake, Insurance and [more...]

Date: 2010-04-23 14:13:07


Coralville's IDT Partners with Marshall University

The Marshall Institute for Interdisciplinary Research (MIIR) has announced it has entered into an applied research and product development partnership with leading biotechnology company Integrated DNA Technologies (IDT). According to the terms of the agreement, scientists at MIIR will be developing optimized biomolecular analyses or “assays” to be used by IDT for the detection and quantification of ribonucleic acid (RNA). The goal of the co-development project is to significantly improve the specificity of IDT’s current assay methods without substantially increasing the cost. IDT’s [more...]

Date: 2010-05-21 20:59:12


Lab Tech / Research Associate (Torrey Mesa) | PCR Jobs

Candidate must be familiar with basic molecular biology techniques such as DNA purification, DNA gel electrophoresis, restriction digestion, PCR including primer design, plasmid construction, DNA sequence analysis software and bacterial transformation. Candidate will also assist with sample receipt and tracking, maintain inventory and equipment, and perform general lab maintenance and cleaning to include handling of biological waste. Education/ Skills/Attributes Required: · Must be a US Citizen. · BS degree in biology/biochemistry with 0-3 years industrial experience. · Experience with [more...]

Date: 2010-10-12 19:15:31


DNA sequencing » Blog Archive » Real Time PCR

Real Time PCR Primer Sets Validated primer sets for quantitative Real Time PCR – Only $24.95. Real-time polymerase chain reaction – Wikipedia, the free Real time quantitative PCR uses fluorophores in order to detect levels of gene expression. Real-time PCR can also be applied to the detection and quantification Real Time PCR Tutorial In real-time PCR, we usually use a reverse transcriptase that has an endo H activity. So how do we use real-time PCR to quantitate the amount of DNA or cDNA? Real Time PCR Expertise in dye chemistries and oligonucleotide labeling has allowed Invitrogen to [more...]

Date: 2010-08-13 02:02:13


Research Associate (San Diego) | PCR Jobs

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:  Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.  Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.  Prepares technical reports, summaries, protocols and quantitative analyses.  Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]

Date: 2010-10-01 19:07:24


DNA Preparation of Bone Marrow Sample for Backtracking | Cancer ...

To isolate DNA from supernatant, the 500µl of 2X concentration of PBS is used.4. 500µl of PCI was added into the tube for 30 minutes.5. Later, the tube was spun at 10000X ϑ for 10 minutes.6. The aqueous supernatant was removed to a new tube without disturbing the PCI layer.7. The PCI layer was discarded and add in 1:10 volume of 10M NH4OAc into the tube to mix.8. Next, the 2X volume of ethanol was added and mixed up the ethanol and precipitated DNA.9. The ethanol was removed from the mixture. The precipitation was washed one time with 500µl 70% ethanol.10. Again, the ethanol was removed [more...]

Date: 2010-07-16 08:22:19



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