Our blogs and videos choice: dna purification

JoVE: Electroeluting DNA Fragments (Video Protocol)

Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution from glass or silica particles, DEAE-cellulose membranes, "crush and soak method", electroelution or very often expensive commercial purification kits. Thus, selecting a method will depend mostly of what is available in the laboratory. The [more...]

Date: 2010-09-05 22:00:00

Blog posts (60) | Videos (23)

DNA Art Blog » Blog Archive » PCR Process

We use primer sets that are optimally designed to span the specific genes you choose to have isolated for your canvas. Our technique focuses on the prevention of pre-PCR mis-priming and primer dimerization. Our primers are designed using software with algorithms that check for the potential of secondary structure formation and annealing of primers to itself or within primer pairs. We use hot start Taq Polymerase is designed to enhance the specificity, sensitivity, and yield of the target DNA sequence. Following PCR amplification and purification of the template DNA, we use restriction [more...]

Date: 2010-10-12 15:19:03

Plant RNA/DNA Purification Kit

Total DNA and RNA from 50 mg of tobacco, tomato and peach were simultaneously isolated with Bio-Synthesis's Plant RNA/DNA Purification Kit (with the optional on-column RNase treatment). Next, 2µL of DNA from each 150 µL elution was mixed in 20 µL of PCR reaction and used in a real-time PCR (95ºC for 3min and 40 cycles at 95 ºC for 15 sec. and 60 ºC for 30 sec.). DNA from tobacco (green), tomato (red) and peach (blue) was successfully amplified, indicating the high quality of the DNA for downstream application. Primer dimer (black) also was shown. Figure 3. Isolate High Quality Total RNA [more...]

Date: 2010-08-16 04:44:00

Quick and reliable DNA purification and sequencing

Keep up with the latest research, events and funding news. Add the  observer,  dissemination and  funding widget to your website or blog The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems.  Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting Station, a high-speed precision pipettor. With its versatile shelf configuration options, the [more...]

Date: 2010-07-28 13:03:49

Plasmid double locus sequence typing for IncHI2 plasmids, a ...

Plasmid double locus sequence typing for IncHI2 plasmids, a subtyping scheme for the characterization of IncHI2 plasmids carrying extended-spectrum β-lactamase and quinolone resistance genes Aurora García-Fernández and Alessandra Carattoli* Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy *Corresponding author. Tel: +39-06-49903128; Fax: +39-06-49387112; E-mail: alecara{at}iss.it Received October 9, 2009. Revision requested January 25, 2010. Revision received February 26, 2010. Accepted March 4, 2010. Abstract Objectives IncHI2 [more...]

Date: 2010-03-31 07:00:00

New ligands for plasmid DNA affinity purification

Molecular therapies and vaccines based on purified plasmid DNA (pDNA) are increasingly being established as promising alternatives to medical classical treatments. The expected wide application of these therapies requires the large-scale production and purification of plasmids. Recent years, had witness the development of new methods for plasmid purification, based on aqueous two-phase systems (ATPS) offering additional advantages like technical simplicity and the use of inexpensive equipment and chemicals. This project is going to study the molecular recognition mechanism and binding [more...]

Date: 2010-06-05 13:04:07

Clean Genes: Michigan Tech Chemist Culls the Good Synthetic DNA ...

Synthetic DNA purification process developed by Shiyue Fang, Michigan Tech Chemistry Department image July 22, 2010— Birds do it, bees do it. Even scientists in labs do it. But the scientists can’t hold a candle to the birds and the bees, who can make gobs of primo DNA without even thinking about it.DNA is a critical element of gene therapy, and scientists working to develop cures for diseases make it with synthesizers. Unfortunately, synthesizers don’t do nearly as good a job as cells in stringing nucleic acids together to make DNA, and many of the resulting sequences—called [more...]

Date: 2010-07-22 19:46:37

CIENCIASMEDICASNEWS: Recurrent Granulibacter bethesdensis ...

DNA was isolated from human tissue by using the Maxwell 16 Tissue DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. DNA concentrations were measured by using a UV spectrophotometer (NanoDrop, Wilmington, DE, USA). The 16S rRNA and methanol dehydrogenase subunit 1 (GeneID YP_744165.1) genes of G. bethesdensis were analyzed by using a PCR and primer sequences 16S-forward: 5'-TCGGGTGGGCACTCTAAAGG-3', 16S-reverse: 5'-GCATCACTGCCTAGCTTCCC-3', MDH-forward: 5'-CCGCAATACGGTCAATTCG-3', and MDH-reverse: 5'-GCCGATCTTCCAGGTTTCTTC-3'. Each reaction mixture [more...]

Date: 2010-09-02 23:42:00

Research Scientist

Research Scientist Sep 14, 2010 Posting date: April 2010 Description: Seeking energetic and meticulous Research Scientist to lead a team in developing DNA purification protocols and real-time PCR assays to support Spartan’s real-time DNA analyzer. Responsibilities will include collaborating with industry and research partners, managing technicians, and writing technical notes and publications. Microbiology, virology and experience in food safety an asset. Qualifications: Ph.D. in Biological Sciences Peer-reviewed publications Extensive experience with molecular biology techniques, [more...]

Date: 2010-09-14 22:04:55

Research Technician

Research Technician Sep 14, 2010 Posting date: April 2010 Description: Seeking energetic and meticulous Research Technician to develop DNA purification protocols and real-time PCR assays for Spartan’s DNA analyzers. Responsibilities will include designing, performing, and analyzing experiments. Qualifications: B.Sc. in Biological Sciences Experience with molecular biology techniques, preferably including real-time PCR Strong interpersonal and communication skills To apply, please forward your resume to [more...]

Date: 2010-09-14 22:08:03

iGEM UT-Tokyo(東大)実験ノート - 9/2 purification of DNA by ...

Experimenter Kariyazono, Nakatake, Zen Abstract purification of location sequence(digested by X & P on 9/2) by electrophoresis & extraction of bands from the gel Protocols 1.separating of DNAs through electrophoresis ←direction sample1 sample2 sample3 sample4 nothing(for 3 lanes) sample3′ sample4′ sample1′ 2.Extraction of DNAs Result;consentration of DNA sample1 5.3ng/ul sample2 7.8ng/ul sample3 6.9ng/ul sample4 7.3ng/ul sample1′ 7.8ng/ul sample3′ 5.8ng/ul sample4′ 6.8ng/ul flpe(PCR②) 3.6ng/ul stored in the [more...]

Date: 2010-09-02 04:27:41

Lab Research Technician (LRA I) (Duke University Medical Center ...

-Must be skilled in basic molecular biology techniques including DNA purification, PCR, and cloning Ideal skills: -Experience working with rodents, including colony maintenance and embryology -Experience with mammalian cell culture and microscopy -Two-year commitment preferred If you are interested please Apply here to Lab Research Analyst Req#400439061 (https://sjobs.brassring.com/1033/ASP/TG/cim_jobdetail.asp?partnerid=25017&siteid=5172&AReq=33156BR). Please include your CV and contact information for three references. For more information, please refer to our website at: [more...]

Date: 2010-10-11 18:43:41

Endofree Plasmid DNA purification using EMD Fractogel DMAE -650(M)

Precipitate DNA with 0.6-1 vol isopropanol. 10, Carefully pour off as much supernatant as possible, then spin down DNA pellet. 11. Wash pellet with 70-80% ethanol, dissolve DNA in TE or water. 12. Regenerate column by successively applying 1 M NaOH, 2-3 M NaCl, and then either water or  storage solution (100 mM NaCl, 20% ethanol).  Store column at 4 ̊C. P1: 6.1g Tris base 3.7g EDTA•2H2O add water to ~800 ml adjust pH to 8.0 with HCl. Add 100mg of RNase A that had been dissolved in 10ml TE and boiled 15min to inactivate contaminating DNase. Add water to 1 L. P2: 8g NaOH 10g SDS [more...]

Date: 2010-09-25 01:57:59

I will start to do DNA experiment on Indonesian local jelly fish ...

I will start to do DNA experiment on Indonesian local jelly fish (including DNA and protein extraction)? Question Is there any suggestion what is the key point/step for DNA and protein isolation and purification protocols from jellyfish? Please suggest some good references, too. Thank you Answer Well. This isn't a thing that you should ask on Yahoo! answers. I think that you should ask somebody which really knows what he's talking about, especially if it is a practical experiment and not just a curiosity. As the other guy said you should see a professor from the [more...]

Date: 2010-09-21 14:23:26

Bio Saga Blog: 5-Day Hands-on Workshop on Molecular Biotechnology ...

Recombinant DNA Technology: Cutting & pasting of DNA molecules (i.e. restriction digestion, ligation, DNA gel eectrophoresis, and gel extraction.) Bioinfo part: Bioinformatics of DNA database / SequenceAnalysis: Pairwise sequence alignment, Multiple sequence alignment, Pattern search.Tools: BLAST, CLUSTALW/X, CLC Bio Main Workbench Genetic Engineering: Transformation and plasmid purification, cloning and sub-cloning, amplification of DNA fragments by polymerase chain reaction (PCR). Bioinfo part: Designing of PCR primers In-silico cloning Searching for homologous and paralogous [more...]

Date: 2010-09-29 05:41:00

MO BIO Laboratories, Inc. DNA Isolation Products Used to Analyze ...

27 /PRNewswire/ — MO BIO Laboratories, Inc., the leader in soil, water and microbial nucleic acid purification, announced today that the company’s DNA isolation products were used to analyze DNA from microbes sampled from the oil plume created by the Deepwater Horizon explosion.  The study published this week in the journal Science, examined the prevalence of hydrocarbon degrading genes in microbial …Further information: Article [more...]

Date: 2010-08-28 10:43:15

RNA extraction protocols and methods

For chloroform extraction: Generally, I can achieve this hassle free by pipetting the aqueous phase very carefully after chloroform extraction - without going too close to the DNA interphase. Further RNA purification (as I need very high purity for Microarrays) can also be achieved by LiCl precipitation, which is specific for RNA and not protein (hence lowering your A280 reading). Rating: [more...]

Date: 2008-05-20 11:49:08

genomic dna

File type:PDF – Download PDF Reader Higher yields from genomic templates with REDTaq Genomic DNA Genomic or large construct DNA containing target sequence. is digested with a restriction . Genomic DNA Purification Instructor's Manual [more...]

Date: 2010-06-23 09:16:14

Protostadienol synthase from Aspergillus fumigatus: Functional ...

AGCTATCCAAATTAC-30) with 1 lL of plasmid DNA containing the full length cDNA encoding A. fumigatus OSPC as the template. Phusion DNA polymerase (Finnzymes) was used with dNTP. (0.2 mM) in a final volume of 50 lL, according to the manufac- ... MonoFas DNA purification kit (GL Sciences). The second PCR was performed with 10 lL of this fragment as an anti-sense primer, and 1 lL (20 pmol) of the N-terminal primer (AfuOSPC-N-Spe, 50-. CTTGCCTACTAGTATGGCGACAGACAGCAGCATG- 30) [more...]

Date: 2010-01-06 08:00:00

Cytoplasmic & Nuclear RNA Purification Kit

RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]

Date: 2010-08-16 04:58:00

NALP1 in Vitiligo-Associated Multiple Autoimmune Disease — NEJM

DNA was prepared from peripheral-blood specimens with the use of a genomic DNA purification kit (Puregene, Gentra Systems) or from saliva specimens with the use of a DNA self-collection kit (Oragene, DNA Genotek). [more...]

Date: 2007-03-22 05:00:00

Expression, Purification and Activity Assay of the Recombinant ...

about 700 bp was purified by DNA purification kit. Both the purified cDNA fragment of the Fc-COMT gene and pET-30a(+) vector were cut individually by. EcoRI and XhoI The digested products were separately purified using gel purification [more...]

Date: 2010-09-03 08:10:44

Plasmid DNA and transformation questions?

The plasmid purification procedure relies on three main steps to produce a bacterial lysate containing small nucleic acids. The first step uses glucose-Tris-EDT A (GTE). The second uses NaOH/SDS and the third uses sodiumb acetate. [more...]

Date: 2010-02-20 00:17:23

Miniprep plasmid DNA purification using diatomaceous earth or ...

9. Wash metrix twice with 0.35 ml of PE (0 mM Tris; 100 mM NaCl; 2.5 mM EDTA; 55% (v/v) Ethanol; pH 7.5). 10. Allow the packed matrix to completely dry by spinning for another 1~5 minutes. 11, Add 50 ul EB or water, spin to elute [more...]

Date: 2010-09-05 20:19:25

Role of Prostanoid DP Receptor Variants in Susceptibility to ...

Plasmid DNA was purified with the Qiagen plasmid purification system. The effects of constructs (1.5 μg) bearing PTGDR haplotypic variants were studied in transiently transfected A549 cells, grown to 80 percent confluence in six-well [more...]

Date: 2004-10-21 05:00:00

(IUCr) Expression, purification and crystallization of a plant ...

The amplified DNA fragment was digested with BamHI/HindIII and ligated into the BamHI/HindIII sites of the pETDuet-1 vector (Novagen) for expression as fusion protein with a His6 tag at the N-terminus. [more...]

Date: 2010-07-29 07:00:00

(IUCr) Mammalian cell expression, purification, crystallization ...

was amplified by PCR from plasmid DNA (using 5'-TGGTACCGCCACCATGGCAAGACAAGGCTGTCTC-3' and 5'-ACCGGTAATCTCGCTCTCATATGT-3' primers) and the reaction product was cut with Asp718I and AgeI restriction enzymes and cloned into the plasmid [more...]

Date: 2010-08-31 07:00:00

ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...

Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]

Date: 2010-08-25 00:51:13

Mummy of Egyptian Pharaoh Tutankhamun family uncover the mystery ...

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Date: 2010-10-08 05:26:15

Guide to Protein Purification, Second Edition (Methods in ...

The 2eof this classic Guide to Protein Purification provides a clean update to existing methods in the field, reflecting the enormous advances made in the remain two decades. In particular, proteomics, mass spectrometry>, and DNA technology take revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an essential first step in understanding their function. This volume examines the greatest number reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, [more...]

Date: 2010-10-11 01:47:47

Research Associate (San Diego) | PCR Jobs

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:  Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.  Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.  Prepares technical reports, summaries, protocols and quantitative analyses.  Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]

Date: 2010-10-01 19:07:24

GTpure Gel/PCR purification kit procedure 01 — Binding

This is the video to show the procedure of GTpure Gel/PCR purification kit. GTpure Gel/PCR purification kit offer a fast and easy to highly recovery the pure and high quality of DNA in agarose gel. Please visit our web site [more...]

Date: 2010-08-08 04:41:28

Antibody Purification Using Membrane Adsorbers - Results from a ...

Dec 1, 2008 By: Leonardo Giovannoni, PhD, Marco Ventani, Uwe Gottschalk, PhD BioPharm International Volume 21, Issue 12 ABSTRACT Most antibody manufacturers currently use a three-column platform comprising Protein A affinity chromatography for product capture, followed by anion exchange (AEX) chromatography in flow-through mode to extract negatively charged contaminants, and then cation exchange (CEX) chromatography or hydrophobic interaction chromatography (HIC) in retention mode to remove positively charged contaminant species. This article presents a new process that uses membrane [more...]

Date: 2008-12-01 05:00:00

Best 2010 Ebook Collection Part 4

Dean J.R. - - - Extraction Methods for Environmental Analysis Dean M. - - - The Human ATP-Binding Cassette (ABC) Transporter Superfamily Dean M.W. - - - $30 Music School Dean T. - Gleek J. - - Internet Marketing Secrets Revealed Deans G.K. - Kroeger F. - Zeisel S. - Winning the Merger Endgame: A Playbook for Profiting from Industry Consolidation Deans P.C. - - - E-commerce and M-commerce technologies Deb S. - - - Video data management and information retrieval Deb S. (ed.) - Deb S.P. (ed.) - - p53 Protocols Debarre O. - - - Higher-Dimensional Algebraic Geometry Debevec P. - - - [more...]

Date: 2010-10-11 18:41:15

Plasmid DNA Recovery Using Size-Exclusion and Perfusion ...

Finally, a second size-exclusion chromatography step was carried out to purify the plasmid DNA from other small molecular-weight contaminants. Analytical methods proved that the purified plasmid DNA had a purity of 95% after Sephacryl S1000. Plasmid identity was confirmed by restriction enzyme digestion. Biological activity of the purified plasmid was confirmed in vivo; immunized mice developed a positive antibody response against all HCV structural antigens. This procedure offers an alternative to traditional methods that use organic reagents, mutagenic and toxic compounds, and [more...]

Date: 2008-09-01 04:00:00

Plasmid DNA extraction from e.coli for digestion mapping clony ...

Research news, bioproducts, protocols, reagents, forums Plasmid DNA extraction from e.coli for digestion mapping clony screening Plasmid DNA extraction from e.coli for digestion mapping clony screening: This protocol utilize the Qiagen buffer P1, P2 and P3 from Qiagen plasmid purification kit. RNase must be added before use. It is cost efficient since it doesn't use expensive plasmid purification columns. This protocol is only intended for screening positive clony. Once positive clonies are determined, purification from bacterial overnight culture can be done using plasmid [more...]

Date: 2006-08-30 07:00:00

Drinking Purified Water | home made water filtration system

It can cause mutations by altering DNA, suppress immune system function and interfere with the natural controls of cell growth. Chlorinated water can destroy polyunsaturated fatty acids and vitamin E in the body while generating toxins capable of free radical damage (oxidation); Chlorinated water destroy much of the intestinal flora, the friendly bacteria that help in the digestion of food and protect the body from harmful pathogens. Tap water may contain: Lead from soldered pipes. Lead can result in anemia, low birth weight in children, increase blood pressure in adults Microbiological [more...]

Date: 2007-09-12 07:00:00

FFPE RNA/DNA Purification Kit

Bio-Synthesis's FFPE RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Alternatively, the kit can be used to isolate total RNA alone or genomic DNA alone from FFPE tissue samples through varying the protease digestion time and performing optional RNase or DNAse digestions. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic [more...]

Date: 2010-07-26 06:19:00

BIO-SYNTHESIS - Custom Antibody Custom Peptide Synthesis Custom ...

BIO-SYNTHESIS, INC. is a leading life science products company with over 20 years of experience in the design and synthesis of Custom Peptide, small molecules and reagents for small scale research and bulk pharmaceutical trials. Using state of the art technology in our well-equipped laboratories. Emerin Antibody Catalog# : 4031 Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family that includes proteins such as LAP2 and MAN1. Each family member, including Emerin, has an ~40 amino acid LEM-domains that binds barrier-to-autointegration [more...]

Date: 2010-10-13 10:34:00

Nucleic Acid Separation

The separation of mRNA from different varieties of RNA wants a special strategy. The Nucleic Acid Separation beads strategy is being used in this separation technique. The isolation and Nucleic Acid Purification are very a lot important for the delicate reactions. The purification is essential for several experiments. We need pure mRNA for doing RT PCR. The RT is nothing however the technology of DNA from the mRNA utilizing the viral enzyme reverse transcriptase. The newly introduced system of protein sure with magnetic beads shall be very helpful in addition to yield very pure particles of [more...]

Date: 2010-09-30 12:19:53

Nucleic Acid Separation

The separation of mRNA from different forms of RNA wants a particular strategy. The magnetic beads strategy is getting used in this separation technique. The isolation and Nucleic Acid Purification are very a lot vital for the sensitive reactions. The purification is essential for several experiments. We want pure mRNA for doing RT PCR. The RT is nothing but the technology of DNA from the mRNA using the viral enzyme reverse transcriptase. The newly launched system of protein sure with magnetic beads will probably be very helpful in addition to yield very pure particles of our requirement. The [more...]

Date: 2010-09-30 12:17:08

AquaStar Plus! UV Water Treatment Device_2060

Unsafe water іѕ placed іח tһе bottle аחԁ exposed tο UV-C light, wһісһ ԁаmаɡеѕ tһе DNA аחԁ RNA іח tһе pathogens, rendering tһеm non-infective. AquaStar Flow Through treats water іח Ɩаrɡеr batches. A small water-purification service саח generate income wһіƖе helping .... mukeh111 on The New HP Envy 17 — in 3D! materiel on Q&A: Cheap place to buy Acer Computer Aspire AS7730-4931 17 Notebook PC? rezster0900 on Q&A: What is the difference between a notebook and a laptop? [more...]

Date: 2010-10-13 09:56:06

Antigen/Antibody Discovery Research Scientist (Sorrento Mesa, San ...

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! CTK Biotech Inc, a leading IVD biotech company located in San Diego area, is seeking an Antigen/Antibody Discovery Research Scientist to join a group of scientists and technicians in the R&D Department. The current position will play a major role in researching and discovering high quality and specific diagnostic reagents to meet the rapidly growing demand of the point-of-care IVD products worldwide. Essential Duties and Responsibilities: Note: Other duties may be assigned. •Perform cloning and [more...]

Date: 2010-10-12 03:53:43

تقنية البلمرة الجزيئية » تعرف عن عملية استخلاص شريط الحمض النووي ...

The VX Viral DNA/RNA Purification CorProtocol™ 25101 allows for walk-away automated preparation of low copy viral DNA/RNA from low total nucleic acid-yielding samples.  Extracted viral DNA/RNA is of high quality and suitable for a wide variety of downstream applications. The default sample volume is 200   µL but can be scaled up to 500 µL with the use of adjustments to this protocol coupled with an alternative run file ( VX_RNA_DNA_CorProtocol_250_500ul_25101_06.CAS4 ) on the instrument.  Increased volumes of certain reagents are required for the extraction of sample volumes greater [more...]

Date: 2010-07-09 16:20:26

IDT enters exclusive agreement with 454 Life Sciences to provide ...

IDT enters exclusive agreement with 454 Life Sciences to provide custom primers for GS FLX Titanium Chemistry 4 October 2010 – HPLC purified and MS certified primers for genome sequencing CORALVILLE, IA: Integrated DNA Technologies (IDT), the custom biology company, has signed an exclusive agreement with 454 Life Sciences, a Roche company, to provide researchers with custom primers for the GS FLX Titanium Chemistry, used on the company’s GS FLX System and GS Junior System. Available as FusionPrimers or Rapid Library MID (Molecular Identification) Adaptor Oligos, these products are used [more...]

Date: 2010-10-04 16:16:19

When should I include the RNase treatment step during DNA ...

i’m not really sure about this so ask for a second opinion =] if you extract the components of the nucleus, you will of course get not only DNA but also RNA and some other components. proteinase is to degrade the other protein components that came with the extraction such as DNA polymerase and the like, including DNase and RNase. obviously you wouldn’t want DNase to degrade your soon-to-be-purified DNA right? So Proteinase K is added for the degradation of the other nucleic components and, most importantly, DNase. RNase is used to degrade the RNA so treating the system with this will [more...]

Date: 2010-09-01 08:00:23

Strategies for Protein Purification and Characterization: A ...

Strategies for Protein Purification and Characterization: A Laboratory Course Manual Product Description Investigators who have identified and cloned a gene of interest often want to isolate and characterize the protein product, yet the methods required are notoriously difficult for the inexperienced. This manual has been adapted from a course held at the Cold Spring Harbour Laboratory in New York, to teach scientists how to execute the major protein techniques by applying them to four distinct, representative types of molecule: a regulatory protein, a DNA-binding protein, a recombinant [more...]

Date: 2010-10-12 10:47:54

Purification of Plasmid DNA

This further purified DNA is suitable for techniques such as DNA sequencing, subcloning or the production of gene probes. In order to purify plasmid DNA after the isolation process, any residual RNA and contaminating protein are removed. This purification step involves two main steps, which are, first, removing residual RNA by using RNase in order to digest RNA and, second, extract contaminating protein using organic solvents, phenol-chloroform. Materials: RNase A: Make up as a solution in water at 10 mg/mL, Heat for 10 min in a boiling water bath or heating block to eliminate any DNase [more...]

Date: 2010-06-26 20:08:11

MicrobeWorld - 10 Tips for the Isolation of High Quality RNA from Soil

RNA Isolation from soil is one of the most difficult applications we perform in environmental molecular biology. RNA purification is always an arduous task and from soil it becomes a bigger challenge. One of the biggest problems is the yield of RNA from soil. Because typical yields of RNA are so much lower than for DNA, usually between 10-20% of the yield of DNA, starting with a larger amount of sample is desired. This requires a method that uses larger and slower processing methods. The other major issue is, of course, the humic acid and inhibitor content of soil co-contaminating the RNA. [more...]

Date: 2010-03-24 05:22:52

Rapid, nondenaturing RNA purification using weak anion-exchange ...

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. [more...]

Date: 2010-01-25 15:06:07

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