EXCLUSIVE: How They Caught Grim Sleeper Suspect Lonnie David ...
Sources tell Los Angeles Weekly that the serial killer was caught through familial DNA testing after his son was arrested and had to give up a DNA swab. A month ago, LAPD detective Dennis Kilcoyne said LAPD was going to try another controversial "familial DNA" matching probe, testing against 1 million samples in the California felon database to find the killer's cousin, brother or uncle. "At least it brings a little bit of closure," Laverne Peters, the mother of victim Janecia Peters, told LA Weekly today. "It is very good news for me. I don't know whether to cry or scream." The science of [more...]
Date: 2010-07-07 21:55:24
The HPV Test - Web Resource
The site provides information about testing for the sexually transmitted human papillomavirus. The website was created by the Digene Corporation, the maker of the HC2 High-Risk HPV DNA Test, which is used to screen for 13 high-risk types of HPV which have been associated with the development of cervical cancer. The test is primarily for women over 30, who are considered to be at greater risk for developing cervical cancer. I would generally warn you to be cautious when using websites created by or for drug/biotechnology companies because they have a product to sell, a good general guideline [more...]
Date: 2006-09-14 02:43:00
Biosensors: Morpholino offers you more
Molecular biologists and geneticists often use DNA microarrays to detect DNA, measure gene expression levels and detect the single-nucleotide polymorphisms that are responsible for various genetic diseases. A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the [more...]
Date: 2010-10-13 09:52:22
Reveo's direct DNA sequencing using tunneling microscopy « The ...
Reveo is developing an ambitious technology to stretch out and deposit taut DNA on conductive surfaces for electronic base detection using one or more STM tips and tunneling current measurements. The linearization and deposition of nucleic acid sequences will likely be done using molecular combing. Reveo’s approach requires atomically flat and positively charged substrate surfaces (e.g. self-assembled monolayers on gold substrates or treated graphite substrates). In addition to molecular combing, Reveo has proposed other methods to linearize DNA, including electrophoretic and hydrodynamic [more...]
Date: 2010-08-25 17:49:12
Lab earns two technology transfer awards in regional competition
Each probe tests for a particular sequence of DNA and small groups of probes can be used to check for specific bacteria or viruses. The current LLMDA has been used to test for more than 2,000 viruses and 900 bacteria. The next generation LLMDA in development will expand that capability to 6,000 viruses, 2,000 bacteria as well as fungi and protozoa organisms. Any probe that detects its specific sequence will fluoresce, which will be read by a scanner and may indicate presence of that organism. The LLMDA team analyzed the safety of rotavirus vaccines and in one case detected a benign pig [more...]
Date: 2010-08-05 15:00:00
Biosensors: Morpholino offers you more
A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the ability to withstand wear and tear over many cycles). Fig. 1: Scanning electron microscopy image (left) of a cluster of parallel SiNWs, and a schematic illustration (right) of the Morpholino-based sensing [more...]
Date: 2010-10-13 12:52:44
20000 Genes Under the Brain: Genes->Brain->Man
To assess gene expression in each small bit of tissue, researchers expose its RNA to a gene chip, or DNA microarray. These small devices are coated with clusters of identical DNA molecules, called probes, within separate areas. Each probe binds to the RNA of a specific gene—the one that contains a complementary set of chemical units, or bases. In a DNA molecule, the base adenine (A) sticks to thymine (T), and guanine (G) pairs with cytosine (C). Thus, the sequence that would bind to the strand illustrated above is (from top to bottom): T, C, C, T, G, C, A. In this way, a chip records which [more...]
Date: 2010-09-02 16:33:00
Neandertal DNA and Proteins
With the completion of the Neandertal genome by the team of Dr. Svante Paabo and a closer look at their proteins by Gregory Hannon’s team at CSHL, scientists reveal incredible similarities between Neandertals and humans. Neandertals, the extinct species of what are most likely our closest relatives, lived on earth at the same time as our human ancestors but died out about 30,000 years ago. With the sequence of their genome now complete, we can compare the DNA to humans and chimpanzees to learn more about what makes humans unique as a species. The discovery of fossils is an exciting link to [more...]
Date: 2010-05-19 13:58:17
Epigenetics Papers: DNA Methylation Analysis by MethyLight Technology
Authors discussed each step for this procedure: (1) determining the site of interest for methylation analysis; (2) methylation-specific primers and fluorogenic probes design; (3) genomic DNA isolation; (4) bisulfite conversion; (5) real- time methylation analysis; and (6) data processing. For primer design, some complications particular to the bisulfite modification should be taken into account: All unmethylated cytosine residues in the genome are converted this reduction in genomic complexity reduces the annealing specificity of PCR primers and fluorogenic probes, which not only [more...]
Date: 2007-08-19 01:26:00
Johne's disease : Diagnosis | The farm's guide
There is a DNA probe recently approved to detect Mycobacterium paratuberculosis in bovine feces. Fecal culture has been the standard test used; it can demonstrate and quantify the presence of the bacteria and, therefore, identify the shedding animal. It is, however, time consuming, expensive to perform, and not widely available. In cattle, cultures usually need to be incubated eight to 16 weeks before growth of the organism is evident. Further-more, animals must be shedding bacteria to be detected. Only a very small proportion of the young stock may be shedding bacteria, and some older [more...]
Date: 2010-09-16 01:34:21
Article Snack » Where To Learn More About Proof Of Paternity
Using the coming of DNA testing these days, paternity can be established with almost 100% accuracy. Except for identical twins, your DNA is as distinctive as your fingerprint. DNA (deoxyribonucleic acid) denotes your genetic makeup like the color of your hair and eyes, skin color, height, and others. The youngster gets 50% of his DNA from his mum and also the other half derives from his dad. Comparing the child’s DNA with that in the biological mom will get rid of the genes inherited from her. What remains are the DNA of the father. A comparison of probes between the father, mother and [more...]
Date: 2010-10-13 06:33:27
gc chlamydia dna probe
Chlamydia / GC, DNA Probe w / Rflx? is a bill that I have in the word "description" It's a long series of tests that the tests are completed routine blood tests. DNA probe assays for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) or commonly called (STD) sexually [more...]
Date: 2010-06-18 08:07:48
Leading Edge DNA Sequencing Method Nets Major NIH
Described in the May 12 online edition of Nano Letters, their novel, highly efficient, optically-based method to detect single DNA molecules in nanopores could significantly reduce the cost of DNA sequencing and the time required to sequence a complete human genome. “We are the first to employ optical detection from individual nanopores, and this allows us to probe multiple pores simultaneously using a single high-speed CCD camera,” said Meller, referring to the charge-coupled devices that science and medical researchers use to obtain high quality images. “As a result, our method can [more...]
Date: 2010-09-13 04:00:00
A day in the life of… Sara
I prepare the appropriate master mixes and primer probes for my samples to prepare them for DNA amplification. I can test for DNA amplification, and therefore if my primers worked, by running an agarose gel electrophoresis on the PCR product against DNA ladder standard material. After sending an electrical current through my gel and buffer, I observe how chunks of DNA from the PCR product have moved (or raced) through the gel relative to the standards (I hope to see a tie at a certain ‘ladder step’ in each lane). At this point in my day I secretly do a celebratory fist pump and heel [more...]
Date: 2010-07-22 23:14:43
fenian32: Colin Duffy sexually violated in prison search, court told
The court was told that DNA samples taken from a seatbelt, in what is believed to have been the getaway car, and the tip of a latex glove that was found in the vehicle, match DNA samples taken from Duffy and therefore link him to the car. An apparent attempt to burn the car, which had been bought 13 days before the attack through Autotrader for £380, had failed. A forensic scientist told the court that the chance of the DNA found on the latex glove belonging to any other person but Duffy was one in a billion. The probe also heard that DNA on a mobile phone and two matchsticks found inside [more...]
Date: 2010-10-13 15:58:36
呼吸器内科医 : CAPの患者ではウイルス検出率が健常人より高い
この検体は12の呼吸器ウイルスをmultiplex TaqMan Hydrolysis probeを用いた リアルタイムPCR(Integrated DNA Technology; Coralville, IA)で解析した。 結果： 少なくとも、1つの呼吸器ウイルスが58人のCAP患者から検出(31.7%)された。 コントロール群では32人 (7.1%)、NPLRTI群では104人(51.7%)。 (それぞれP＜ .01 、P＜ .01） コロナウイルスはCAP患者の24人(13.1%)、コントロール群の17人(3.8%)、 NPLRTI群の21人(10.4%)で認められた。 RSウイルスはCAP群の13 [more...]
Date: 2010-10-06 05:31:42
The Bacillus and Myxococcus Developmental Networks and Their ...
ChIP-chip : DNA from chromatin immunoprecipitation (ChIP) is used to probe a DNA microarray. EBP : enhancer-binding proteins. Endospore : a dormant structure produced within a cell of certain gram-positive bacteria; after release by [more...]
Date: 2007-06-09 07:00:00
Truth probing unethical
That is why many judges will both allow DNA fingerprinting to be admissible as evidence with its one-in-a-billion error and reject polygraph exam results. Even if a flawless test could be created, the use of a brain wave test on interrogation targets is also wrong and should not be done. In the Wired article, Rosenfeld wrote that he wants to see his technology applied to terrorist interrogation, saying a hypothetical terrorist suspect could either be sent to Egypt for the water board or be given a scientifically based test. Unfortunately, the use of brain wave technology to detect deception [more...]
Date: 2010-09-14 02:19:47
DNA sequencing » Blog Archive » DNA Analysis
DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a DNA profiling involved restriction enzyme digestion, followed by Southern blot analysis. DNA fingerprint: Definition from Answers.com DNA fingerprint n. An individual’s unique sequence of DNA base pairs, determined by exposing a sample of the person’s DNA to molecular [more...]
Date: 2010-08-31 16:03:47
Genetic Technologies Ltd.?s Non-coding DNA Patent Upheld on ...
s (GTG's) patent for non-coding DNA technologies. U.S. Patent No. 5,612,179 titled "Intron sequence analysis method for detection of adjacent locus alleles as haplotypes" claims a method for facilitating the detection of specific gene variations. The third party initiated ex parte re-examination Control No. 90/010,318, was filed on October, 15, 2008 requesting that each of claims 26-32 be found invalid in view of a new reference, which was not previously cited in the prosecution of the '179 patent; namely a printed publication titled "Comparison of Multiple HLA-A Alleles at the [more...]
Date: 2010-07-13 16:50:00
Stadt Schaffhausen: Polizei ermittelt 23j. Schmuckdieb schweiz ...
04.10.2010 Stadt Schaffhausen: Polizei ermittelt 23j. Schmuckdieb schweiz. Nationialität - DNA-Probe wegen Körperverletzung führte zu ihm (SHPOL/URA) Die Schaffhauser Polizei hat einen jungen Mann ermittelt, der in dringendem Tatverdacht steht, im Dezember 2007 Schmuck aus einem Schaukasten in der Stadt Schaffhausen gestohlen zu haben. Am 19.12.2007 wurde die Scheibe eines Schaukastens eines Schmuckgeschäftes in der Löwengässchen-Unterführung in der Stadt Schaffhausen eingeschlagen und daraus Schmuck im Wert von über 2'300.- Franken gestohlen. Am 27.07.2010 wurde ein 23-jähriger [more...]
Date: 2010-10-07 15:58:07
8 murder suspects brought to HKL for DNA profiling
The eight people helping police probe the so-called Banting murders were brought to the Kuala Lumpur Hospital today for what is believed to be DNA profiling.They were brought to the Emergency and… Read the original here: [more...]
Date: 2010-09-22 16:22:33
Company Profile : Diagnostic supplier : Suyog Diagnostics : Mumbai ...
The company is representing world renowned Diagnostics Companies and selling Diagnostics Kits for Coagulation disorders, Viral Markers for HIV, HBV, HCV, HDV, HEV, Biochemistry, Chromatography kits etc. Life Science Division: The company is representing world renowned Diagnostics Companies and selling Chemicals, Bio - Chemicals, FISH Probes, Radio Chemicals, Reagents, Cell Bio, Electrophoresis grade, Neuro chemicals, Molecular Biology grade, DNA Extraction kits, PCR Kits, Restriction Enzymes, DNA Ladders & modifying enzymes, Proteomics, Cell Culture reagents, Plant Tissue Culture Grade [more...]
Date: 2010-07-09 15:29:00
Scientist Solutions - DNA MicroArray Basics
Just came across this resource today, while looking up MicroArray companies. Its pretty nice and a great beginner step. DNA microarrays can be thought of as the massively parallel version of Northern blotting. While the Northern blotting technique is capable of analysing for each experiment the expression of one single gene in different conditions, microarrays allow the exploration of the expression levels of thousands of genes in several different conditions in one single run. As Northern blotting and many other classic techniques widely used in molecular biology, the fundamental basis of [more...]
Date: 2009-03-19 19:31:20
Malaysia Chronicle: Slow pace in Sosilawati murder probe to be ...
Kubang Kerian member of Parliament Salahuddin Ayub cited that the DNA report had yet to be available, saying any DNA report should be out within a week after wrapping up of investigation. “This shows that there are still some missing links for the police to act. A professional DNA report by any chemist department should be out within one, or two or three days. "But here we see that even the DNA report is not available yet,” said Salahuddin, and recalled that the police’s investigation papers handed to the Attorney General on September 23 had been returned to the police with specific [more...]
Date: 1998-09-20 07:00:00
PhD Project to study molecular Biophysics of DNA and Proteins at ...
The research department Materials Physics within the Institute for Materials Research IMO at Hasselt University offers the following mandates (m/f): 2 PhD students (2 x 2 years) in the field of molecular biophysics of DNA and proteins (mandates 101/32/02 and 101/32/03) Both mandates are situated within the project ‘Molecular wires – probing and understanding their electronic and mechanical properties’ funded by the Special Research Funds BOF of Hasselt University. The focus is on the understanding of the kinetics and dynamics of the interactions between DNA-fragments and between [more...]
Date: 2010-10-07 11:47:22
Doch DNA-Test in Sexaffäre - Innsbruck - Chronik - Tirol ...
In diesem Fall aber doch, zumal sich der ehemalige Toppolizist monatelang weigerte, einer „erkennungsdienstlichen Behandlung“ (Abnahme der Fingerabdrücke und einer DNA-Probe) zuzustimmen. Wie die Tiroler Tageszeitung bereits berichtete, [more...]
Date: 2010-09-25 00:13:13
Healthcare Sales & Marketing Network: James Godsey, Ph.D., to Join ...
Mr. Jones added that "Dr. Lorincz has led Digene's research since the company's founding. He was responsible for the development of the company's lead product - the Hybrid Capture® 2 HPV DNA Test. By expanding our scientific leadership team, Dr. Lorincz will have more time to foster our relationships with global scientific thought leaders and to explore possible new 'frontiers' for the company's growth." In addition to his work at Veridex and GenProbe, Dr. Godsey was President/COO of ThermoGenesis Corporation and he held a number of increasingly responsible positions with DADE Microscan [more...]
Date: 2006-10-27 21:26:00
Progenika Receives CE Mark for First DNA Chip to Detect Mutations ...
The Leading Information Source for Chronic Disease Research and Management Metabolic Type I Diabetes Type II Diabetes Neuropathy Retinopathy Thyroid Disorder Addisons Disease Dialysis Kidney Disorders Obesity Cardiovascular Hypertension Cardiac Arrythmias Coronary Artery Disease Heart failure Angina Central Nervous System Parkinson's Disease Schizophrenia ADHD Anxiety Depression Epilepsy Obsessive Compulsive Disorder Alzheimer Dementia Fibromyalgia Lou Gehrig's Respiratory Asthma Bronchiectasis Cystic Fibrosis Chronic Obstructive Airways [more...]
Date: 2010-09-27 08:54:00
Thrombosis Journal | Full text | Expression of sterol regulatory ...
DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]
Date: 2009-02-18 08:00:00
Dna Honda Accord 1986-2002 Coilover Lowering Spring Kit
Lowering Springs and Kits Drop your ride a couple inches with custom tuned lowering kits for your specific vehicle, improve handling and looks! LIGHTING 3RD BRAKE LIGHT BUMPER LIGHT CORNER LIGHT HEAD LIGHT TAIL LIGHT XENON LIGHT BULBS HID LIGHT BULBS PERFORMANCE AIR FILTER CARBON FIBER MUFFLER CATBACK EXHAUST COLD AIR INTAKE DOWN PIPE HEADER SHORT AIR INTAKE STAINLESS MUFFLER TEST PIPE TURBO MANIFOLD COOLING SYSTEM INTERCOOLER INTERCOOLER PIPING RADIATOR TURBO CHARGER T3/T4 TURBOCHARGER T04E TURBOCHARGER GT35R TURBOCHARGER K03 TURBOCHARGER K04 TURBOCHARGER TD05H TURBOCHARGER T25/28 [more...]
Date: 2010-10-10 16:33:38
Virology Journal | Full text | Integration of bovine herpesvirus 4 ...
Alternatively, the signal associated with the host genomic fraction might be due to large, complex concatameric viral genome replication intermediates or to mishybridization of the probe with host genomic DNA. However, neither alternative seems to be the case, because such signal was absent from the genomic fraction of acutely infected control cells. 2) Using an alternative approach to determine the status of the BoHV-4 genome in persistently infected cells, based on Southern blot analysis of the complete cellular genome digested with a suitable restriction enzyme and hybridised with a [more...]
Date: 2010-09-21 00:00:00
Comments: Life Technologies buys Ion Torrent « Omically Speaking
There was some exciting news yesterday from the next-generation sequencing (NGS) field, with the buy-out of Ion Torrent - a start-up that’s developed a novel DNA sequencing machine – by the life-sciences behemoth Life Technologies (for a whopping 350million dollars). The technology for sequencing of DNA – the blueprint of all life – is at an exciting stage. Ten years ago, the complete human genome was sequenced at the cost of several billion dollars and decades of attempt. Today, a full genome can be sequenced for as little as $10,000 and in less than a week. The price and time are [more...]
Date: 2010-08-18 17:13:21
Groups across the country are helping to facilitate a nationwide tour of Erasing David – a documentary about privacy, surveillance and the database state. Phil Booth, our National co-ordinator is hitting the road with David Bond the film maker and producer Ashley Jones to help raise awareness of the victims of the Database State that lie behind the headlines of privacy abuses. Tour Locations: University of East Anglia, 11th November, Time 7pm Manchester – to be confirmed Glasgow – to be confirmed + ‘Erasing David’ screenings + The producers of Erasing David are offering [more...]
Date: 2010-10-11 11:07:09
Judge to review conviction in Texas arson-murder case – This Just ...
A Texas judge is set to consider whether a man who was executed for setting a fire that killed his children was wrongly convicted on what his supporters call "junk science." State District Judge Charles Baird granted a request from Cameron Todd Willingham's family for a hearing to investigate whether he was wrongfully convicted using flawed science. The family's petition, filed September 24 in Travis County, also asks the court to examine whether the stateÂ failed to adequately consider potentially exculpatory evidence before putting Willingham to death in 2004. The lawsuit asks the court [more...]
Date: 2010-10-05 23:14:16
Christianity.com - Blogs - Conversations with Your Favorite ...
The whole day was very instructive because I didn't know much about my family's history. I knew that my father was from Mississippi and my mother was Iowa, and I knew they met in Nome, Alaska while serving in the Army in World War II. During the reunion a local researcher gave us a fascinating lecture on the the history Mayfield and Pritchard families during the last 200 years. The various branches intertwined at several points across the generations, in part because the families lived close together in the small rural communities that make up north Mississippi. During the afternoon we [more...]
Date: 2010-10-11 09:43:00
HHMI News: Timothy Harris to Deliver Public Talk at Janelia Farm
The event is free and open to the public, but tickets are required for admission. Directions for obtaining the tickets are available on the HHMI web site at www.hhmi.org/janelia/events.html. Seating is limited to 250 people. The lecture is the seventh in a series called “Dialogues of Discovery at Janelia Farm.” Past speakers in the series have included Thomas R. Cech, former president of HHMI and an HHMI investigator at the University of Colorado, Boulder; Roian Egnor, a fellow at the Janelia Farm Research Campus; Ronald M. Evans, an HHMI investigator at the Salk Institute for Biological [more...]
Date: 2010-10-04 17:01:00
Microfluidics and Rapid DNA Analysis « The Writer's Forensics Blog
A recent report from a research group in Birmingham, England and the Center for Applied NanoBioscience and Medicine at the University of Arizona College of Medicine presented a new rapid method for DNA analysis. The researchers state that with a new DNA processing cartridge that they have developed, DNA results can be obtained in as little as four hours. This could be extremely useful to law enforcement though it does have a few drawbacks. After obtaining the analysis I would assume that it would then be uploaded into the CODIS system for comparison. The problem is that CODIS is not complete [more...]
Date: 2010-08-16 15:29:41
Ovarian Cancer Diagnostics | Ovaire
DNA testing reagents for cancer CTC Test markets Biochip Innovations New companies with new approaches "Laggards" Companies that have not met expectations Protein important molecular markers used in tissue staining Biomarkers under investigation Mergers and Acquisitions Status of pharmacogenomic tests Microarrays Markets in clinical laboratories The lists of suppliers and main products Unmet market needs This report includes market analysis of the following tests: The Profile Cancer CTC, fecal occult blood, PSA, H. pylori, IHC, ISH, and evidence of specific cancers: Bladder [more...]
Date: 2010-10-11 18:38:23
Ultrafast EMCCD cameras point the way to faster DNA sequencing
To harness this powerful single-molecule detection modality for high-throughput DNA sequencing, each of the four nucleotides (A, C, G and T) in the target DNA has to be converted into a predefined sequence of oligonucleotides, using a technique called Circular DNA Conversion. Each pre-defined sequence is then hybridized to a self-quenching molecular beacon.In their experimental setup, the Meller team recorded DNA translocation through a 4nm pore in a SiN membrane immersed between two aqueous solutions. With the camera in place, the researchers were able to detect florescence bursts in two [more...]
Date: 2010-06-10 07:00:00
ChIP Enrichment Analysis can speed up drug discovery
Email Subscription Enter your email address to subscribe to this blog and receive notifications of new posts by email. Blog Stats 96,578 hits Blogroll Get on Live chat with me WordPress.com WordPress.org blogs I read biohacker Biotech Blog Biotech Life Eye on DNA Gene Genie Gene Sherpa Open access News POSTED RIN Science Roll Scientific Blog Youth Curry links vote for Nextweb awards microarray Global Health Vision MicrobiologyBytes UK Web Focus offers Rate my Blog Rate my Blog Clinical Search India's silicon valley Bangalore in trouble as State's governer imposes President Rule, due to [more...]
Date: 2010-09-16 06:45:50
ERV's latest blog on the FDA paper. One step too far?.
PNAS paper, using standard PCR to ID 'positives' and 'negatives', with insane numbers of bands as a result of non-specific primer binding. Insane numbers. As if this is 1990 and we dont know what 'Real Time PCR' and 'Taqman Probes' are. Im going to ignore that and grant the premise they really have IDed viral sequences. And they arent XMRV. They dont cluster anywhere near XMRV on a phylogenetic tree. Theyre something else entirely. Okay, well, logically it could be contamination from mouse DNA. So they checked for that... by looking for mouse mitochondrial DNA. *blink* I understand [more...]
Date: 2010-08-23 07:00:00
Methylation Test May Aid Colon Cancer Diagnosis (CME/CE) | Discuss ...
To develop a more sensitive test for DNA methylation, investigators employed a form of technology known as BEAMing (beads, emulsion, amplification, and magnetics). They performed PCR amplification of individual DNA fragments covalently linked to magnetic beads suspended in water-based nanoparticles immersed in oil droplets. The beads contained a DNA sequence specific for exon 1 of the vimentin gene, which is hypermethylated in colon cancer. Following PCR amplification, the beads were hybridized by means of fluorescent probes specific to the state of methylation and then sorted and analyzed by [more...]
Date: 2010-10-09 11:00:27
The Quality Stocks Stock Newsletter For Smallcap Companies Blog ...
Shivji noted the 15 years of genomic research and leadership experience of Dr. Schroth, and his tireless efforts to advance gene expression techniques using DNA sequencing, microarrays, PCR and next-gen sequencing, all of which has resulted in numerous publications and patents spanning the entire field of genomic analysis. Dr. Schroth expressed his excitement over the opportunity to push the SmartChip platform forward as a perfect solution for multiple global research efforts that are focused on making improved and more targeted therapeutics, and called the SmartChip system “the most [more...]
Date: 2010-10-12 17:57:42
NO2ID Supporters' Newsletter No. 159 – 11th October 2010 « William ...
Terri Dowty, ARCH – Subject: Children’s Databases Helen Wallace, Genewatch – Subject: DNA Database Bertrand Russell Room, Conway Hall; 25 Red Lion Square, London WC1R 4RL http://www.conwayhall.org.uk/where.htm _*+ Manchester +*_ For information on Manchester NO2ID see manchester.no2id.net/ or contact Dave Page on firstname.lastname@example.org or 07722 615 398. **_*+ Norwich +*_ *+ 11th November – Erasing David Screening With Special Guests +* On Thursday 11th November a showing of the fascinating documentary film ‘Erasing David’ is taking place at the UEA, ARTS 01.02, starting 7.00pm. The [more...]
Date: 2010-10-13 11:36:36
Gene Sequencing: DNA analysis
Not everyone can properly and correctly analyze the DNA. Apartyears of study devoted to knowledge of DNA, DNA analysts to go through years of experience and practice in laboratories and practice of DNA testing and analysis in order to analyze the DNA and how it works. The training of DNA analysts understanding of sophisticated tools and machines developed for DNA analysis. proper management and use of these devices would make them more efficient in the pursuit of DNA to be analyzed correctly. Most of the DNA consists of 23Pairs of chromosomes, mitochondrial DNA inherited from the mother, and [more...]
Date: 2010-09-22 05:00:00
New nanopore method for DNA sequencing
(Nanowerk News) Sequencing DNA could get a lot faster and cheaper and thus closer to routine use in clinical diagnostics thanks to a new method developed by a research team based at Boston University. The team has demonstrated the first use of solid state nanopores tiny holes in silicon chips that detect DNA molecules as they pass through the pore to read the identity of the four nucleotides that encode each DNA molecule. In addition, the researchers have shown the viability of a novel, more efficient method to detect single DNA molecules in nanopores. "We have employed, for the [more...]
Date: 2010-05-21 06:27:21
Genotyping of Chlamydia trachomatis strains from culture and ...
Mol Cell Probes . 2010 Oct 7; Ruettger A, Feige J, Slickers P, Schubert E, Morré SA, Pannekoek Y, Herrmann B, de Vries HJ, Ehricht R, Sachse K Current typing methods of Chlamydia (C.) trachomatis are mainly based on the diversity of the ompA gene, which is coding for the major outer membrane protein A. The present study aimed at facilitating genotyping of strains of this obligate intracellular human pathogen by developing a DNA microarray assay using the ArrayTube™ format for individual samples and the ArrayStrip™ format for higher throughput. See the original post here: Genotyping of [more...]
Date: 2010-10-12 21:11:53
Revised Nationality Law | Japan Probe
The envisioned amendment would enable nationality to be given to any child born out of wedlock as long as he or she receives paternal recognition. Update: The Japan Times has more details about DNA tests and the new nationality rules. Akihabara News – Gadgetry from Japan (Subscribe) dannychoo.com – Your portal to Japan (Subscribe) Kirainet.com – A geek in Japan (Subscribe) 16 comments - What do you think? Posted by James - December 4, 2008 at 7:15 am Categories: Foreigners in Japan [more...]
Date: 2008-12-03 22:15:52
Mitochondrial DNA rearrangement associated with fertility ...
Comparison of the mitochondrial DNA restriction patterns of CMS-Sprite, the three fertile revertants, and the five restored lines revealed loss of a 6.0-kilobase (kb) Pst I fragment in all restored and revertant lines. Southern hybridizations with a 1.3-kb BamHI clone, internal to the 6.0-kb Pst I fragment, as a probe revealed two configurations of 6.0-kb homologous sequences in the sterile cytoplasm; one of the configurations was lost upon reversion or restoration. Mitochondrial DNA rearrangement has thus been observed upon restoration by a nuclear restorer gene in this CMS [more...]
Date: 2010-09-29 13:30:31