Our blogs and videos choice: dna polymerase

Variants of PCR (1)

The bacteriophage T4 DNA polymerase was also tested shortly after the first reports of PCR. It has a higher fidelity of replication than the Klenow fragment. Since it is also destroyed by heat, it has seen little use since the development of thermostable polymerases. The DNA polymerase from Thermus aquaticus (or Taq), was the first thermostable polymerase used in PCR, and is still the one most commonly used. The enzyme can be isolated from its 'native' bacterial source, or from a cloned gene expressed in E. coli. The Stoffel fragment is produced from a truncated gene for Taq polymerase, [more...]

Date: 2008-09-11 03:56:00

Blog posts (112) | Videos (21)
 


Error-Prone DNA Repair Activity during Somatic Hypermutation in ...

Duplications and deletions were associated with N additions, suggesting participation of a DNA polymerase with some degree of template independence during the repair of DNA breaks initiated by activation-induced cytidine deaminase. Other mutations were consistent with some in vitro activities of mammalian translesion DNA polymerase η: tandem base substitutions, strand slippage, and small insertions/deletions. The nature of substitution patterns shows that DNA lesions at shark Ig genes recruit DNA repair factors with a species-specific repertoire of activities. We speculate that the tandem [more...]

Date: 2010-10-07 06:53:59


Forensic Serology Information Page

What is Forensic Serology?According to forensic serologist Marcella Jones of www.ForensicMentor.com, forensic serology is the analysis of body fluids as they relate to forensic cases, including DNA analysis. Accordingly, the role of the forensic serologist involves:Examining evidence for the presence of body fluids e.g. blood, semen, hair, tissue, saliva, feces, and urineEvaluating evidence for potential DNA analysisEvaluating species of body fluidsPerform PCR (polymerase chain reaction) based typing of STR (short tandem repeat sequences) of genomic DNA, or mitochondrial sequence analysis of [more...]

Date: 2010-02-04 08:00:00


DNA fingerprinting simplified

One of the most frequently used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic technique often used in genetic or DNA fingerprinting. The procedure is achieved by moving negatively charged nucleic acid molecules through a gelatinous substance known as agarose by using an electric field. Andrew, in collaboration with his father and other researchers, completed the study, which is published in the January 2008 issue the American Society of Horticultural Science’s journal HortTechnology. The youngest author ever to publish in an ASHS journal, Andrew [more...]

Date: 2008-05-14 04:52:12


My Helical Tryst: Single Molecule DNA Sequencing

There are a number of companies that have recently launched competing technology platforms that are capable of sequencing DNA at the single molecule level. You may ask, why? Well, the answer is simply cost-effectiveness. DNA sequencing technology has been around for several decades, and to date, the Human Genome Project (HGP) is considered the pinnacle application of that technology. The cost of the HGP was approximately $3 billion and took about a decade and half. It was the molecular biology equivalent of putting a man on the moon. But what is the next step? Well, if we can systematically [more...]

Date: 2009-02-11 22:17:00


PCR Rap | sciencerapper

An ingenious technique called Polymerase Chain Reaction (PCR, in the labaratory vernacular) uses a Polymerase to selectively an cheaply amplify certain sequences of DNA from very small amounts of starting materials.  The invention of PCR piggy-backe don many existing technologies, and we have used PCR to learn many, many things about biology. The following rap about the Polymerase Chain Reaction that highlights a few things: the beautiful way in which DNA is copied (using enzymes called Polymerases) the great insight of Kary Mullis to create a useful tool that could copy DNA in monumental, [more...]

Date: 2010-09-04 14:18:04


Herpes Helper Activities for Adeno-Associated Virus Replication

The present study was conducted to integrate these observations and to further explore the requirement of other HSV-1 proteins during early AAV replication steps, i.e. rep gene expression and AAV DNA replication. Using a cellular model that mimics AAV latency and composite constructs coding for various sets of HSV-1 genes, we first confirmed the role of ICP0 for rep gene expression and demonstrated a synergistic effect of ICP4 and, to a lesser extent, ICP22. Conversely, ICP27 displayed an inhibitory effect. Second, our analyses showed that the effect of ICP0, ICP4, and ICP22 on rep gene [more...]

Date: 2009-06-25 08:00:06


DNA polymerase

DNA store information within an organism which is passed down to generation after generation through DNA replication and sexual/asexual reproduction. There are no known organisms extinct or alive that have used another system of inheritance. That being said, DNA is its replication is the most important biological molecular process on the planet second only to perhaps photosynthesis that generates the bulk of the energy stores for the worlds ecosystems and the organisms within them. Formore interesting videos from project ‘Curiousity’ check out: www.youtube.com Our website: [more...]

Date: 2010-10-08 04:26:00


Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...

Genetic Fingerprinting of Bacillus thuringiensis Isolates by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)(Published in Nepal Journal of Science and Technology 10(2009) 97-103)Gyan Sundar Sahukhall, Upendra Thapa Shrestha1, Binod Lekhak2, Anjana Singh2, Viswanath Prasad Agrawal11Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal. 2Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepale-mail: gyan633413@gmail.com AbstractRandom Amplified Polymorphic DNA (RAPD) is a method of producing a genetic fingerprint of a [more...]

Date: 2010-01-21 14:06:00


Marine Drugs | Free Full-Text | Antiviral Lead Compounds from ...

It inhibits viral DNA polymerase and DNA synthesis of herpes, vaccinica and varicella zoster viruses. However due to the discovery of new types of viruses and emergence of drug resistant strains, it is necessary to develop new antiviral lead compounds continuously. Several sponge derived antiviral lead compounds which are hopedto be developed as future drugs are discussed in this review. Supply problems are usually the major bottleneck to the development of these compounds as drugs during clinical trials. However advances in the field of metagenomics and high throughput microbial cultivation [more...]

Date: 2010-10-11 07:00:00


Responding to Merlin Part III – Merlin's Delineation Between ...

In bacteria, this is often done by increasing transcription rates using a mutagenic DNA polymerase. In your immune system this is done, at least in part, by a deaminase attaching to the antibody gene to cause mutations. This deaminase is targeted by a non-coding DNA element, which points it not only to the correct gene, but the correct *part* of the correct gene. It would make no sense to mutate the part of the antibody which attaches to the cell, but only the part which attaches to the antigen. And, in fact, that is precisely the part that gets mutated. The region which attaches to the [more...]

Date: 2010-09-08 04:41:08


"Polymerase Chain Reaction (PCR)" Biology Animation Library ...

"Polymerase Chain Reaction (PCR)" Biology Animation Library :: Dolan DNA Learning Center: "Polymerase Chain Reaction Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA. This animation is featured in our 'Spotlight Collection' on Polymerase Chain Reaction, along with video interviews with Kary Mullis, a 3D molecular animation of PCR, and several laboratory [more...]

Date: 2010-08-23 17:54:00


EpiCentral: Examining blocking lesions in ancient DNA

Blocking lesions prevent amplification and sequencing of affected molecules, thus limiting the analysis of DNA derived from ancient samples. Heyn et al. recently developed a new method--polymerase extension profiling (PEP)--that reveals occurrences of polymerase stalling on DNA templates. This sequencing-based technology allows detection of damage on a single-molecule level. The technique used CircLigase™ ssDNA Ligase for high-efficiency ligation of single-stranded adaptors (containing the Roche 454 A sequence) to the 3’ ends of primer-extension products. The authors found evidence of [more...]

Date: 2010-10-07 20:29:00


WHAT HAPPENS WHEN 2 REPLICATION BUBBLES MEET?

So what will happen to the leading and lagging strand? Please post anything that you know here. Anything at all. Thank you. It’s greatly appreciated. Related posts:DNA Replication Cartoon Our second cartoon made for our AP Biology class.... How can DNA synthesis proceed in both directions from a replication origin? Even though DNA polymerase can synthesis DNA in only one... How to define leading strand in DNA replication? I’ve searched online about the leading strand and found a... Why does the lagging strand require more primers than the leading strand in DNA replication? Why does the [more...]

Date: 2010-05-25 17:15:18


Wiley::Fundamentals of Biochemistry: Life at the Molecular Level ...

5. Manipulating DNA A. Cloned DNA Is an Amplified Copy B. DNA Libraries Are Collections of Cloned DNA C. DNA Is Amplified by the Polymerase Chain Reaction D. Recombinant DNA Technology Has Numerous Practical Applications. Box 3-1 Pathways of Discovery ..... B. The Pyruvate Dehydrogenase Complex Catalyzes Five Reactions 3. Enzymes of the Citric Acid Cycle A. Citrate Synthase Joins an Acetyl Group to Oxaloacetate B. Aconitase Interconverts Citrate and Isocitrate [more...]

Date: 2008-06-20 04:00:00


Mechanistic Studies with DNA Polymerases Reveal Complex Outcomes ...

Biochemical analysis of DNA polymerase activity has shown that these enzymes utilize a series of molecular checkpoints that typically promote formation of “Watson-Crick” base pairing geometry and, therefore, stable propagation of DNA [more...]

Date: 2010-08-19 07:00:00


Technology Review: A Portable DNA Detector

A new portable DNA analyzer performs real-time analysis of blood samples left at the scene of a crime. Researchers at the University of California, Berkeley, developed the device, which packs microfluidics, electronics, optics, and chemical detection technology into a single briefcase-sized unit. "While previous groups have developed lab-on-a-chip systems, none of them have succeeded in making a completely portable, robust system that can be used at a scene," says team leader Richard Mathies. On-site CSI: After a blood sample is collected from the scene of a crime, this briefcase-sized [more...]

Date: 2008-09-24 04:00:00


Science Webulagam: Ribonucleic acid (RNA)

Ribonucleic acid (RNA) is a biologically important type of molecule that consists of a long chain of nucleotide units. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. RNA is very similar to DNA, but differs in a few important structural details: in the cell, RNA is usually single-stranded, while DNA is usually double-stranded; RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom); and RNA has the base uracil rather than thymine that is present in DNA. RNA is transcribed from DNA by enzymes called RNA [more...]

Date: 2010-04-27 13:28:00


INCURVATA2 Encodes the Catalytic Subunit of DNA Polymerase {alpha ...

The strong icu2-2 and icu2-3 insertional alleles caused fully penetrant zygotic lethality when homozygous and incompletely penetrant gametophytic lethality, probably because of loss of DNA polymerase activity. The weak icu2-1 allele carried a ... D. Schuermann, O. Fritsch, J. M. Lucht, and B. Hohn Replication Stress Leads to Genome Instabilities in Arabidopsis DNA Polymerase {delta} Mutants PLANT CELL, September 1, 2009; 21(9): 2700 - 2714. [more...]

Date: 2007-10-30 10:30:47


Blood test detects HIV/AIDS in infants aged 6 months in India DNA ...

Unlike other tests, the HIV DNA PCR test does not rely on the presence of antigens or antibodies in the blood for diagnosis. Instead, it identifies genetic material by highlighting sequences of the virus within the subject’s DNA. All babies born to mothers with HIV are born with HIV antibodies. The currently available tests can predict the chances of HIV infection in infants borne to infected mothers only after the completion of 18 months. This delay in diagnosis often caused a problem for doctors because they found starting the treatment using the life-saving Anti-Retroviral Treatment [more...]

Date: 2010-07-19 22:55:54


Biology Animations: animation of DNA replication

The chain elongation reaction catalyzed by DNA polymerases is a nucleophilic attack by the 3’-OH group of the primer on the innermost phosphorus atom of the deoxynucleoside triphosphate. A phosphodiester bridge forms with the concomitant release of pyrophosphate. The subsequent release of pyrophosphate by pyrophosphatase helps drive the polymerization forward. ***Elongation of DNA chain proceeds 5’-to-3’ direction. ***Template directed enzyme also have nuclease activity. Maurice Wilkins-Rosalind Franklin (x-ray diffraction photos of fibers of DNA) Erwin Chargaff (nucleotide ratios in [more...]

Date: 2007-10-12 15:35:00


.ada.aimi.kisah: dna replication.ape tu?

in this chapter, there's three processes to synthesis protein from DNA. replication(DNA>DNA)> Transcription(DNA>RNA) > Translation(RNA>Protein) just focus on Eukaryotes jea tau. prokaryotes tak masuk dalam syllabus. :) replication of DNA - separation of double strands of DNA is by Helicase. and to prevent the rewinding of the strands, there is SSBp.(Single strand Binding protein) to help. - Leading strand (5' to 3') is copied by DNA Polymerase III which functioned as template for the replication. - lagging strand ( 5' to 3') is produced by DNA Polymerase III and form Okazaki Fragment. [more...]

Date: 2010-08-18 19:04:00


Journal of Autoimmune Diseases | Full text | Autoantigenic nuclear ...

POLDIP3 binds to DNA and RNA during replication process, is a specific target of S6 kinase 1, interacts with p50 subunit of DNA polymerase delta, possess duplex DNA-unwinding and ATPase activities and regulates cell growth . The four autoantigenic proteins reported are of nuclear localization. Sizes of the above mentioned clones, genes that encode them, chromosome in which they are and data of the proteins are shown in Table [more...]

Date: 2008-07-14 00:00:00


Pharmalot… Pharmalittle… Good Morning // Pharmalot

There are two main technologies involved: RT-PCR (reverse transcription polymerase chain reaction) and DNA microarray. What research scientists in universities and cancer centers have been doing for the past ten years is to try and figure out a way to use this technology to look for patterns of gene expression which correlate with and predict for the activity of anticancer drugs. However, genes do not operate alone within the cell but in an intricate network of interactions. Since the new millenium there has been the increasing acceptance of the concept that cancer is a very heterogenous [more...]

Date: 2010-10-11 11:40:52


Investigations of the Inner Workings of T4 Polymerase: the Work of ...

Investigations of the Inner Workings of T4 Polymerase: the Work of Stephen J. Benkovic Abstract Template-Primer-dependent Turnover of (Sp)-dATPαS by T4 DNA Polymerase. The Stereochemistry of the Associated 3′ → 5′-Exonuclease (Gupta, A., DeBrosse, C., and Benkovic, S. J. (1982) J. Biol. Chem. 257, 7689–7692) Spatial Relationship between Polymerase and Exonuclease Active Sites of Phage T4 DNA Polymerase Enzyme (Gopalakrishnan, V., and Benkovic, S. J. (1994) J. Biol. Chem. 269, [more...]

Date: 2009-10-09 13:36:18


DNA Polymerase and other factors

DNA polymerase can add free nucleotides to only the 3’ end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, therefore, needs a primer at which it can add the first nucleotide. Primers consist of RNA and DNA bases with the first two bases always being RNA, and are synthesized by another enzyme called primase. An enzyme known as a helicase is required to unwind DNA from a double-strand structure to a [more...]

Date: 2008-07-27 11:03:00


DNA Transcription (Advanced)

Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT The Central Dogma of Molecular Biology: “DNA makes RNA makes protein” Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place… inserting it with [more...]

Date: 2010-10-03 03:02:09


Bio Saga Blog: 5-Day Hands-on Workshop on Molecular Biotechnology ...

Recombinant DNA Technology: Cutting & pasting of DNA molecules (i.e. restriction digestion, ligation, DNA gel eectrophoresis, and gel extraction.) Bioinfo part: Bioinformatics of DNA database / SequenceAnalysis: Pairwise sequence alignment, Multiple sequence alignment, Pattern search.Tools: BLAST, CLUSTALW/X, CLC Bio Main Workbench Genetic Engineering: Transformation and plasmid purification, cloning and sub-cloning, amplification of DNA fragments by polymerase chain reaction (PCR). Bioinfo part: Designing of PCR primers In-silico cloning Searching for homologous and paralogous [more...]

Date: 2010-09-29 05:41:00


[Dna: Replication, Repair, Recombination, and Chromosome Dynamics ...

In most cells, 100-1000 Okazaki fragments are produced for each replicative DNA polymerase present in the cell. For fast-growing cells, this necessitates rapid recycling of DNA polymerase on the lagging strand. Bacteria produce long Okazaki fragments (1-2 kb) and utilize a highly processive DNA polymerase III (pol III), which is held to DNA by a circular sliding clamp. In contrast, Okazaki fragments in eukaryotes are quite short, 100-250 bp, and thus the eukaryotic lagging strand polymerase does not require a high degree of processivity. The lagging strand polymerase in eukaryotes, polymerase [more...]

Date: 2008-10-17 07:00:00


How can we get cancer if DNA Polymerase corrects errors.?

If our DNA Polymerase corrects all the errors in base pairing in our DNA than how do we get cancer? If im not wrong we get cancer because the cell builds the wrong protein due to wrong nitrogenous bases. Thanks! [more...]

Date: 2010-09-18 03:49:19


Role of Telomeres in the Development of Cancers | Fashion in US

This detail alone causes problems due to the fact that DNA replication progresses 5′ to 3′ which leads to discontinuity in the lagging strand, therefore the nucleotide adding enzyme DNA polymerase cannot finish the polymerisation process ... A small piece of DNA is left unreplicated at the end of each cell cycle which results in that amount of DNA to be lost and if this process carries on the way it does, then this will eventually cause the essential, protein-coding [more...]

Date: 2010-10-13 15:26:08


PCR: when you need to find out who the daddy is. | Neurotic Physiology

And now enter the Taq polymerase. The Taq polymerase is a version of DNA polymerase that is found in bacteria (Thermus aquaticus) living in hot springs in places like Yellowstone. Places where you have to be REALLY tough to live. [more...]

Date: 2010-08-31 04:18:56


Strand Displacement by DNA Polymerase III Occurs through a τ-ψ-χ ...

In addition to the well characterized processive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA templates, the enzyme possesses an intrinsic strand displacement activity on flapped templates. [more...]

Date: 2009-11-06 14:36:37


A quick 5 second animation of the chemistry involved in DNA synthesis

Click here for the short animation: http://bioweb.uwlax.edu/GenWeb/Molecular/Theory/DNA_synthesis/dnasynth.mov And here's the explanation: When DNA is synthesized the free 3´ hydroxyl (OH) group from the growing strand of DNA attacks the a phosphate on the next base to be added (see the red arrows below). Pyrophosphate is released and the new base forms a phosphodiester bond with the growing strand of DNA. The free 3´ hydroxyl group is then free to attack the next base to be added. This reaction is catalyzed by DNA polymerases. Similar reactions are catalyzed by RNA polymerases in the [more...]

Date: 2009-02-19 07:13:00


A Mathematical Model for DNA Damage and Repair

DNA polymerase (Pol) catalyzes the release of the deoxyribose phosphate residue and DNA synthesis to fill the gap, which is then sealed by DNA ligase (Figure 1). This reaction sequence is carried out in one of two pathways, [more...]

Date: 2010-09-06 19:42:23


PNAS: 噬菌体DNA复制机制:TPs organize the early viral DNA ...

Importantly, we show that in the absence of the TP N-terminal domain the efficiency of ϕ29 DNA replication is severely affected. Moreover, the TP recruits the phage DNA polymerase to the bacterial nucleoid, and both proteins later are redistributed to enlarged helix-like structures in an MreB cytoskeleton-dependent way. These data disclose a key function for the TP in vivo: organizing the early viral DNA replication machinery at the cell nucleoid. Published online before print September 7, 2010, doi: 10.1073/pnas.1010530107 PNAS September 21, 2010 vol. 107 no. 38 [more...]

Date: 2010-09-28 15:52:00


DNA Polymerase: Structural Homology, Conformational Dynamics, and ...

Klentaq1, the analogous Klenow fragment portion of thermophilic Thermus aquaticus DNA polymerase I, showed a C-terminal fold nearly identical to that of Klenow fragment and can be seen in Figure 1(a) . The polymerase domain can be [more...]

Date: 2010-09-27 19:49:46


Translesion Synthesis Polymerases in the Prevention and Promotion ...

These mutations are formed when residual, unrepaired DNA damage stalls progression of the replication fork during S phase. Stalled replication forks are most frequently resolved using error-free mechanisms that include homologous ..... C. W. Lawrence, P. E. M. Gibbs, R. S. Murante et al., “Roles of DNA polymerase ζ and Rev1 protein in eukaryotic mutagenesis and translesion replication,” in Proceedings of the Cold Spring Harbor Symposia on Quantitative Biology, vol. [more...]

Date: 2010-08-13 07:00:00


Association between the Herpes Simplex Virus-1 DNA Polymerase and ...

Herpes simplex virus-1 (HSV-1) is a large dsDNA virus that encodes its own DNA replication machinery and other enzymes involved in DNA transactions. We recently reported that the HSV-1 DNA polymerase catalytic subunit (UL30) exhibits [more...]

Date: 2010-08-27 14:59:07


Structure and mechanism of human DNA polymerase eta.

Structure and mechanism of human DNA polymerase eta. Nature. 2010 Jun 24;465(7301):1044-8 Authors: Biertümpfel C, Zhao Y, Kondo Y, Ramón-Maiques S, Gregory M, Lee JY, Masutani C, Lehmann AR, Hanaoka F, Yang W The variant form of the human syndrome xeroderma pigmentosum (XPV) is caused by a deficiency in DNA polymerase eta (Poleta), a DNA polymerase that enables replication through ultraviolet-induced pyrimidine dimers. Here we report high-resolution crystal structures of human Poleta at four consecutive steps during DNA synthesis through cis-syn cyclobutane thymine dimers. Poleta acts [more...]

Date: 2010-06-26 11:02:58


Genome Biology | Full text | Chromatin dynamics and the regulation ...

Despite these tight constraints, nucleosomes must be able to allow the DNA sequences to be accessible to DNA-binding proteins and to the action of 'molecular machines' such as DNA and RNA polymerases, ATP-dependent nucleosome .... pool by briefly labeling newly synthesized histones with a heavy isotope, such as 15N or 13C. Histone modifications can then be determined by MS for both the old and new histones, based on the mass difference between these two pools of histones. [more...]

Date: 2010-10-08 00:00:00


JAK2 V617F Mutation in Unexplained Loss of First Pregnancy — NEJM

Meta-analyses and reviews1 suggest that disorders promoting venous thrombosis, collectively termed “thrombophilias,” contribute to the pathogenesis of fetal loss. The high variability among studies with respect to the associated risk between such ... Women were screened by means of a nested allele-specific polymerase-chain-reaction assay performed on DNA extracted from circulating leukocytes. Multiparametric conditional logistic-regression analysis was performed. [more...]

Date: 2007-11-07 21:55:30


Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...

RAPD-PCR mixture was set up that contained 20-50 ng of genomic DNA, 40 pmol of primer, 1 U of Taq polymerase (Bangalore GENEI.), 200 uM (each) deoxynucleoside triphosphate, 10 mM Tris-Cl (pH 8.3), 50 mM KCl, 3 mM MgCl2 and 1% DMSO. Each reaction mixture was overlaid with 25 ul of mineral oil and amplified with a Perkin-Elmer Cetus DNA Thermal Cycler model TC-1 as follows: (i) Initial denaturation, 1 cycle consisting of 5 min at 94oC and (ii) 35 cycles, with 1 cycle consisting of 1 min at 94oC, 2 min at 36oC, and 3 min at 72oC, followed by a final extension step at 72oC for 10 min. RAPD [more...]

Date: 2009-09-25 16:32:00


Regulation of DNA polymerase POLD4 influences genomic instability ...

Dear Colleagueaaa, Here is an article you may find interesting. Elucidation of a C-Rich Signature Motif in Target mRNAs of RNA-Binding Protein TIAR. http://biowizard.com/abstract.php?pmid=17682065 Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA Mol Cell Biol. 2007 Aug 6; PMID: 17682065 BioWizard [more...]

Date: 2010-09-30 18:42:02


Molecular Biology Lecture III Prof. Graham Walker Video | DnaTube ...

This is what's known as the leading strand because DNA, the synthesis of the new strand can go -- Which is going 5 prime to 3 prime is going in the same direction as the movement of the replication fork. ... nAnd it's continually jumping. And I told you that the little RNA primer is used to start each strand. And then the DNA polymerase is able to elongate that. And then at the end these little nicks in here, the RNA has to be removed, fill in the gap and then it's sealed [more...]

Date: 2009-02-04 08:00:00


(IUCr) Structure of BT_3984, a member of the SusD/RagB family of ...

The gene encoding BT_3984 (GenBank NP_812895; Swiss-Prot Q8A0N7) was amplified by polymerase chain reaction (PCR) from B. thetaiotaomicron VPI-5482 genomic DNA using PfuTurbo DNA polymerase (Stratagene) and I-PIPE (Insert) primers [more...]

Date: 2010-09-22 07:00:00


Characterization of a Y-Family DNA Polymerase eta from the ...

Similar experiments were performed in a study on TLS past a benzopyrene adduct by another thermostable Y-family DNA polymerase, archaeal Dpo4, although the organic solvents were added to stabilize the benzopyrene moiety in the [more...]

Date: 2010-06-29 07:00:00


What is the role of enzymes in the DNA replication process?

C i think, I thought it could be A but the last words say from scratch but enzymes like dna polymerase require primers to start the base sequence so further bases can be read so it cant be A Know better? Leave your own answer in the comments! This entry was posted on Tuesday, October 5th, 2010 at 3:16 am and is filed under Enzymes. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own [more...]

Date: 2010-10-05 10:16:50


Long Mate Pair library construction backgrounder - SEQanswers

Only a couple of data points here, but they suggest that there is something intrinsic to the genomic DNA that drastically alters the nick translation speed of the DNA polymerase being used. We are making a new library this week... [more...]

Date: 2010-06-22 20:37:20


Interactive

Welcome to the Interactive Section of the site. Here we will be posting papers and other replication news. Please feel free to add your comments to any of the postings here. Active Site Mutations in Mammalian DNA Polymerase {delta} Alter Accuracy and Replication Fork Progression Michael W. Schmitt, Ranga N. Venkatesan, Marie-Jeanne Pillaire, Jean-Sebastien Hoffmann, Julia M. Sidorova, and Lawrence A. Loeb J. Biol. Chem. 2010 285: [more...]

Date: 2010-10-11 08:02:00



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