The Bacillus and Myxococcus Developmental Networks and Their ...
ChIP-chip : DNA from chromatin immunoprecipitation (ChIP) is used to probe a DNA microarray. EBP : enhancer-binding proteins. Endospore : a dormant structure produced within a cell of certain gram-positive bacteria; after release by [more...]
Date: 2007-06-09 07:00:00
How is a DNA microarray different that gel electrophoresis?
I know that they are both used to kind of sort DNA and whatnot, but what’s different? Best answer: Answer by _haze_ A DNA microarray (also commonly known as gene or genome chip, DNA chip, or gene array) is a collection of microscopic DNA spots, commonly representing single genes, arrayed on a solid surface by covalent attachment to a chemical matrix. DNA arrays are different from other types of microarray only in that they either measure DNA or use DNA as part of its detection system. Qualitative or quantitative measurements with DNA microarrays utilize the selective nature of DNA-DNA or [more...]
Date: 2010-07-29 20:16:32
DNA microarrays to monitor the health of farmed fish
The antibody microarray is the arrangement of antibodies on a small plate of glass, each antibody being able to recognize and bind specifically to a protein in the blood during an infection . When an antibody has bound to a protein present in the blood sample , a reagent fluoresces red or green . This allows to visualize the presence and extent of infection. As for the protein chip , it is a piece of litmus paper on which are disposed of proteins characteristic of several diseases that may affect the flounder ( eg Edwardsiellose ) . If the fish is or has been affected by a disease, it has [more...]
Date: 2010-09-06 15:24:16
What are gel elctrophoresis and DNA microarray? How is each used ...
Question by neonpinkispink: What are gel elctrophoresis and DNA microarray? How is each used in the study of genetics? Best answer: Answer by brainybroad269 Gel electrophoresis is the use of a gel to separate pieces of DNA (or RNA, or protein) under an electric field. The simplest form separates pieces by size. More complex (2-dimensional) techniques can separate proteins by size and charge. A DNA microarray is a collection of short DNA sequences (“oligonucleotides”) immobilized on a piece of glass or other support (the “gene chip”). When you incubate the chip with your experimental [more...]
Date: 2010-09-25 04:09:26
Technology Review: Searching for Disease Genes Gets Easier
David Barker: The on the chip were chosen to represent the common variation in the genome mapped in the HapMap. The microarray does a good job of capturing all that variation and can help discover genes that control risk of major diseases, such as cancer, asthma, diabetes, and heart disease. TR: What kinds of projects are under way?DB: We are working with an Icelandic company, DeCode, which will genotype half the population of Iceland and will look for associations in all kinds of diseases (see "Genomics into Drugs").Lots of other projects are in progress. Children's Hospital [more...]
Date: 2006-12-13 08:00:00
Epigenetic Transgenerational Actions of Vinclozolin on Promoter ...
A methylated DNA immunoprecipitation with methyl-cytosine antibody followed by a promoter tilling microarray (MeDIP-Chip) procedure was used to identify 52 different regions with statistically significant altered methylation in the sperm promoter epigenome. Mass spectrometry bisulfite analysis was used to map the CpG DNA methylation and 16 differential DNA methylation regions were confirmed, while the remainder could not be analyzed due to bisulfite technical limitations. Analysis of these validated regions identified a consensus DNA sequence (motif) that associated with 75% of the promoters. [more...]
Date: 2010-10-02 03:04:02
Stem cells, obesity finding lead Nobel predictions (Reuters) | MMC ...
For the chemistry prize, to be awarded on October 6, Pendlebury favors Patrick Brown of Stanford University School of Medicine in California, who invented DNA microarrays, also known widely as gene chips, which are broadly used by [more...]
Date: 2010-10-07 13:13:17
DNASTAR Releases Next-Gen RNA-Seq and ChIP-Seq Software - SEQanswers
Currently, DNASTAR produces Lasergene, a sequence analysis software suite; SeqMan NGen for the assembly of second and third generation DNA sequence data; ArrayStar, a microarray gene expression visualization and analysis package; QSeq, an RNA-Seq and ChIP-Seq data alignment and analysis tool integrated with ArrayStar; and GenVision, a data visualization software [more...]
Date: 2010-09-22 20:01:13
Scientist Solutions - DNA MicroArray Basics
Just came across this resource today, while looking up MicroArray companies. Its pretty nice and a great beginner step. DNA microarrays can be thought of as the massively parallel version of Northern blotting. While the Northern blotting technique is capable of analysing for each experiment the expression of one single gene in different conditions, microarrays allow the exploration of the expression levels of thousands of genes in several different conditions in one single run. As Northern blotting and many other classic techniques widely used in molecular biology, the fundamental basis of [more...]
Date: 2009-03-19 19:31:20
MEMS Works.com ― [This Domain is for sale] Microfluidics - CPR ...
Microscale behavior of fluids Silicone rubber and glass microfluidic devices. Microscale behavior of fluids Silicone rubber and glass microfluidic devices. Top: a photograph of the devices. Bottom: DIC micrographs of a serpentine channel ~15 m wide. The behavior of fluids at the microscale can differ from 'macrofluidic' behavior in that factors such as surface tension, energy dissipation, and fluidic resistance start to dominate the system. Microfluidics studies how these behaviors change, and how they can be worked around, or exploited for new uses. At small scales (channel diameters of [more...]
Date: 2010-07-30 07:00:00
ChIP Enrichment Analysis can speed up drug discovery
Email Subscription Enter your email address to subscribe to this blog and receive notifications of new posts by email. Blog Stats 96,578 hits Blogroll Get on Live chat with me WordPress.com WordPress.org blogs I read biohacker Biotech Blog Biotech Life Eye on DNA Gene Genie Gene Sherpa Open access News POSTED RIN Science Roll Scientific Blog Youth Curry links vote for Nextweb awards microarray Global Health Vision MicrobiologyBytes UK Web Focus offers Rate my Blog Rate my Blog Clinical Search India's silicon valley Bangalore in trouble as State's governer imposes President Rule, due to [more...]
Date: 2010-09-16 06:45:50
End of Microplates? Novel Electronic Biosensing Technology Could ...
End of Microplates? Novel Electronic Biosensing Technology Could Facilitate a New Era of Personalized Medicine The multi-welled microplate, long a standard tool in biomedical research and diagnostic laboratories, could become a thing of the past thanks to new electronic biosensing technology developed by a team of microelectronics engineers and biomedical scientists at the Georgia Institute of Technology. Associate professor Muhannad Bakir (left), from Georgia Tech’s School of Electrical and Computer Engineering, holds a prototype electronic microplate, while Professor John McDonald, from [more...]
Date: 2010-09-19 21:30:02
Expression Profiling of PBMC-based Diagnostic Gene Markers ...
Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients Shigeto Kobayashi1, Akihiko Ito2, Daisuke Okuzaki3,4, Hiroaki Onda3,5, Norikazu Yabuta3, Ippei Nagamori3, Kazuo Suzuki6,7, Hiroshi Hashimoto1 and Hiroshi Nojima3,4,5,* 1Department of Rheumatology and Internal Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan 2Division of Molecular Pathology, Department of Cancer Biology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan 3Department of [more...]
Date: 2010-09-04 18:43:52
Lab-on-Chip – ST – Genetic Analysis
The In-Check platform is based on a silicon chip that integrates all the functions needed to identify given oligonucleotide sequences in a sample, including microfluidic handling, a miniaturized PCR reactor and a custom microarray. STMicroelectronics and Veredus Laboratories Team to Diagnose Avian Flu Using Rapid-Detection Point-of-Need [more...]
Date: 2010-10-06 09:16:00
SpringerProtocols: Abstract: The State-of-the-Art of Chromatin ...
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the DNA sequences associated with it. ChIP has been widely used to map the localization of post-translationally modified histones, histone variants, transcription factors, or chromatin-modifying enzymes on the genome or on a given locus. In [more...]
Date: 2009-08-01 04:00:00
DNA sequencing » Blog Archive » Microarray
Find more about Microarray on Wikipedia’s sister projects: A microarray is a multiplex lab-on-a-chip. It is a 2D array on a Genome.gov | DNA Microarray Technology Fact sheet on DNA microarray technology, published by the National Human Genome Research Institute. Microarray Technology: An introduction to DNA Microarray Learn how the microarray technology works: A short tutorial that gives an introduction to the microarray technique and it’s [more...]
Date: 2010-08-19 12:26:07
20000 Genes Under the Brain: Genes->Brain->Man
To assess gene expression in each small bit of tissue, researchers expose its RNA to a gene chip, or DNA microarray. These small devices are coated with clusters of identical DNA molecules, called probes, within separate areas. Each probe binds to the RNA of a specific gene—the one that contains a complementary set of chemical units, or bases. In a DNA molecule, the base adenine (A) sticks to thymine (T), and guanine (G) pairs with cytosine (C). Thus, the sequence that would bind to the strand illustrated above is (from top to bottom): T, C, C, T, G, C, A. In this way, a chip records which [more...]
Date: 2010-09-02 16:33:00
DNA Microarray (Genome Chip) protocols and methods
DNA Microarray (Genome Chip) (archive) This simple, printer-friendly site has been created and maintained by Leming Shi, Ph.D. You'll find the basics on DNA microarray technology and a list of academic and industrial links related to this exciting new technology. Last update 02-May-2001, Rating Good of 2 votes. Comments Read more Discussion Job [more...]
Date: 2001-02-01 08:00:00