Faster DNA Analysis at Room Temperature
Faster DNA Analysis at Room Temperature Researchers in Canada have combined DNA microarrays with microfluidic devices, which are used for the precise control of liquids at the nanoscale. An article in an upcoming issue of the journal Biomicrofluidics, describes how the first combined device can be used for sorting DNA. Read more on Newswise Technorati Tags: Analysis, faster, Room, [more...]
Date: 2010-08-10 03:32:52
Scientist Solutions - DNA MicroArray Basics
Just came across this resource today, while looking up MicroArray companies. Its pretty nice and a great beginner step. DNA microarrays can be thought of as the massively parallel version of Northern blotting. While the Northern blotting technique is capable of analysing for each experiment the expression of one single gene in different conditions, microarrays allow the exploration of the expression levels of thousands of genes in several different conditions in one single run. As Northern blotting and many other classic techniques widely used in molecular biology, the fundamental basis of [more...]
Date: 2009-03-19 19:31:20
Familial Cancer Associated with a Polymorphism in ARLTS1 — NEJM
George Adrian Calin, M.D., Ph.D., Francesco Trapasso, M.D., Ph.D., Masayoshi Shimizu, B.S., Calin Dan Dumitru, M.D., Ph.D., Sai Yendamuri, M.D., Andrew K. Godwin, Ph.D., Manuela Ferracin, Ph.D., Guido Bernardi, Ph.D., Devjani Chatterjee, Ph.D., Gustavo Baldassarre, M.D., Shashi Rattan, B.S., Hansjuerg Alder, Ph.D., Hideaki Mabuchi, M.D., Takeshi Shiraishi, M.D., Lise Lotte Hansen, Ph.D., Jens Overgaard, M.D., D.M.Sc., Vlad Herlea, M.D., Francesca Romana Mauro, M.D., Guillaume Dighiero, M.D., Benjamin Movsas, M.D., Laura Rassenti, Ph.D., Thomas Kipps, M.D., Ph.D., Raffaele Baffa, M.D., Ph.D., [more...]
Date: 2009-06-01 21:13:36
Epigenetic Transgenerational Actions of Vinclozolin on Promoter ...
A methylated DNA immunoprecipitation with methyl-cytosine antibody followed by a promoter tilling microarray (MeDIP-Chip) procedure was used to identify 52 different regions with statistically significant altered methylation in the sperm promoter epigenome. Mass spectrometry bisulfite analysis was used to map the CpG DNA methylation and 16 differential DNA methylation regions were confirmed, while the remainder could not be analyzed due to bisulfite technical limitations. Analysis of these validated regions identified a consensus DNA sequence (motif) that associated with 75% of the promoters. [more...]
Date: 2010-10-02 03:04:02
Natural Health News: Supplement Suppresses Breast Cancer Cells
Expression of cell cycle regulatory genes were determined by DNA-microarray analysis. Effect of BD on invasiveness was assessed by cellular adhesion, migration, and invasion assays. Results. BD treatment of cells MDA-MB-231 resulted in the cytostatic inhibition of cell proliferation with IC50 22.2, 19.1, and 17.5 μg/mL for 24, 48, and 72 hours, respectively. The inhibition of proliferation was mediated by the upregulation expression of CCNG1, CHEK1, CDKN1C, GADD45A, and E2F2, whereas BD downregulated expression of CCNA1 and CDK6 genes. The induction of expression of GADD45A and inhibition of [more...]
Date: 2010-10-11 16:51:00
Free molecular biology software for Macs | Bioinformatics Blog
Posted on the October 12th, 2010 under Free mac software by bioinformatics-blog 1. Serial Cloner Serial Cloner is fantastic all-in-one workbench; import and manipulate sequences, construct plasmid and restriction site maps, determine %GC and fragment TM, extract and ligate fragments, perform virtual PCR… and lots more, all in one window using a very intuitive graphical interface. 2. 4Peaks 4Peaks is an extremely user friendly DNA sequence chromatogram viewer and editor from the extremely talented guys at Mekentosj. It’s miles better than any of it’s clunky counterparts… try it, [more...]
Date: 2010-10-12 11:08:47
Genomics in Endocrinology · DNA Microarray Analysis in Endocrine ...
Services Find all our services for youAds, Reprints & Bulk SalesAuthorsBooksellersBook ReviewsInstructors & LecturersLibrariansRights & PermissionsSocietiesSubscription Agencies We are happy to help you!Discounts & SpringerTokenDVD & CD-ROM SupportManage your AccountOnline Exam CopiesOrder processRights & PermissionsSpringerAlerts & NewsletterSpringer & Open Access Our Email ServicesSpringerAlerts OverviewSpringerAlerts for JournalsSpringerAlerts for Book SeriesSpringerAlerts for BooksSpringerAlerts for LibrariansSpringerAlerts for [more...]
Date: 2007-02-22 08:00:00
Ganit Labs Bangalore Genomics/DNA Microarray Scientist/RA/Lab ...
Ganit Labs Bellary Road, Hebbal, Bangalore 560024 Positions: Scientist/RA/Lab Director/Informatics Manager Genomics Applications and Informatics Technology Labs (Ganit Labs) is a newly formed, Bangalore-based, public private-partnership initiative. Ganit Labs would work in the field of functional genomics and next generation sequencing. Currently we are building a state-of-the art modern genomics lab along with the best computational facility that would present entrepreneurial atmosphere of start-up companies and academic rigor and solid training of research institutes/universities. The [more...]
Date: 2010-08-22 04:26:12
The Quality Stocks Stock Newsletter For Smallcap Companies Blog ...
Shivji noted the 15 years of genomic research and leadership experience of Dr. Schroth, and his tireless efforts to advance gene expression techniques using DNA sequencing, microarrays, PCR and next-gen sequencing, all of which has resulted in numerous publications and patents spanning the entire field of genomic analysis. Dr. Schroth expressed his excitement over the opportunity to push the SmartChip platform forward as a perfect solution for multiple global research efforts that are focused on making improved and more targeted therapeutics, and called the SmartChip system “the most [more...]
Date: 2010-10-12 17:57:42
Microarray Analysis and Tumor Classification — NEJM
There are two basic approaches to generating microarray data. In a two-color array, two samples of RNA, each labeled with a different dye, are simultaneously hybridized to the array (Figure 2Figure 2 Overview of DNA Microarray Analysis. [more...]
Date: 2006-06-07 20:56:39
Predictive Molecular Pathology — NEJM
However, fresh tissue is highly preferable for DNA-microarray analysis, and its use may become mandatory in future assays to allow investigation of the status of drug targets. A global change in the handling of tumor tissue is [more...]
Date: 2009-03-27 14:12:49
GATE 2011 Syllabus for Biotechnology | GATE 2011 Biotechnology ...
Recombinant DNA Technology: Restriction and modification enzymes; Vectors: plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; cDNA and genomic DNA library; Gene isolation; Gene cloning; Expression of cloned gene; Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain reactions; DNA fingerprinting; Southern and northern blotting; In-situ hybridization; RAPD; RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy.w w w.w a y 2 f r e s h e r s . c o m Bioinformatics: Major bioinformatics resources (NCBI, [more...]
Date: 2010-09-01 10:17:23
SemiFind.gr - Συνέδριο XEMET 2010 - 8th Southeast European ...
Analysis and interpretation of results · Common problems during the process. 9.00-14.00 Workshop WEN3: DNA MicroArray Technology in Pharmacogenomics. 9.00-12.00 Theoretical part · Basic principles of Microarray analysis [more...]
Date: 2010-08-19 07:00:00
DNASTAR Releases Next-Gen RNA-Seq and ChIP-Seq Software - SEQanswers
Currently, DNASTAR produces Lasergene, a sequence analysis software suite; SeqMan NGen for the assembly of second and third generation DNA sequence data; ArrayStar, a microarray gene expression visualization and analysis package; QSeq, an RNA-Seq and ChIP-Seq data alignment and analysis tool integrated with ArrayStar; and GenVision, a data visualization software [more...]
Date: 2010-09-22 20:01:13
cDNA Microarray Gene Expression Profiling of Hedgehog Signaling ...
Cell cycle analysis demonstrated accumulation of GANT61-treated cells at the G1/S boundary. cDNA microarray gene expression profiling of 18,401 genes identified Differentially Expressed Genes (DEGs) both common and unique to HT29 and GC3/c1. Analyses using GenomeStudio (statistics), Matlab (heat map), Ingenuity (canonical pathway analysis), or by qRT-PCR, identified p21Cip1 (CDKN1A) and p15Ink4b (CDKN2B), which play a role in the G1/S checkpoint, as up-regulated genes at the G1/S boundary. Genes that determine further cell cycle progression at G1/S including E2F2, CYCLIN E2 (CCNE2), CDC25A [more...]
Date: 2010-10-02 18:05:44
BIVDA.COM - OligoMix® Oligonucleotide Mixture
OligoMix.com LC Sciences provides a unique new tool, OligoMix® enabling low cost, multiplex custom oligonucleotides for various applications serving diverse needs in genomics, proteomics, protein engineering, synthetic biology, drug discovery, and emerging new fields of science such as next-generation sequencing. OligoMix® is a MIXTURE of a large number of DESIGNED synthetic DNA oligonucleotide sequences delivered in a single microtube. OligoMix® is a product developed in response to the need for pooled oligonucleotides used in multiplexing reactions. We synthesize thousands of [more...]
Date: 2010-10-10 21:29:50
Ovarian Cancer Diagnostics | Ovaire
DNA testing reagents for cancer CTC Test markets Biochip Innovations New companies with new approaches "Laggards" Companies that have not met expectations Protein important molecular markers used in tissue staining Biomarkers under investigation Mergers and Acquisitions Status of pharmacogenomic tests Microarrays Markets in clinical laboratories The lists of suppliers and main products Unmet market needs This report includes market analysis of the following tests: The Profile Cancer CTC, fecal occult blood, PSA, H. pylori, IHC, ISH, and evidence of specific cancers: Bladder [more...]
Date: 2010-10-11 18:38:23
ChIP Enrichment Analysis can speed up drug discovery
Email Subscription Enter your email address to subscribe to this blog and receive notifications of new posts by email. Blog Stats 96,578 hits Blogroll Get on Live chat with me WordPress.com WordPress.org blogs I read biohacker Biotech Blog Biotech Life Eye on DNA Gene Genie Gene Sherpa Open access News POSTED RIN Science Roll Scientific Blog Youth Curry links vote for Nextweb awards microarray Global Health Vision MicrobiologyBytes UK Web Focus offers Rate my Blog Rate my Blog Clinical Search India's silicon valley Bangalore in trouble as State's governer imposes President Rule, due to [more...]
Date: 2010-09-16 06:45:50
TriMark Publications Announces Release of Its Nutraceuticals and ...
Packed with the latest information relating to new products and industry trends, this analysis not only quantifies but also qualifies the nutraceuticals market in the areas of research, product development and investment opportunities. ... DNA Sequencing and PCR Markets - Genomics World Markets - High-Growth Diagnostic Tests Markets - Indian Pharmaceutical Industry - Key Diagnostic Testing Markets - Mammography World Markets - Medical Imaging Markets - Microarray Markets [more...]
Date: 2010-10-11 21:42:02
FTDNA Family Finder test
(BUSINESS WIRE)--Family Tree DNA, the first and largest genealogical DNA testing company, today announced the launch of the Family Finder DNA test for connecting family members across all ancestral lines. The test utilizes Affymetrix’ recently launched Axiom™ genotyping technology and the GeneTitan® System to confidently match a wide range of family relationships within five generations. Family Finder represents a major advancement over earlier genealogical tests, which revealed only connections along specific paternal lines (for males) or maternal lines (males or females). [more...]
Date: 2010-05-15 07:13:00
Genome.gov | DNA Microarray Technology Fact sheet on DNA microarray technology, published by the National Human Genome Research Institute. DNA MICROARRAY VIRTUAL LAB DNA microarray analysis is one of the fastest-growing new technologies in the field of genetic research. Find out how DNA microarray analysis is being used to [more...]
Date: 2010-08-19 12:24:23
Gene association analysis: a survey of frequent pattern mining ...
Generation of DNA microarray gene expression data introduces a variety of data analysis issues not encountered in traditional molecular biology or medicine. Frequent pattern mining (FPM) has been applied successfully in business and scientific data for discovering interesting association patterns, and is becoming a promising strategy in microarray gene expression analysis. We review the most relevant FPM strategies, as well as surrounding main issues when devising efficient and practical methods for gene association analysis (GAA). We observed that, so far, scalability achieved by efficient [more...]
Date: 2009-10-08 14:42:23
Chromosomal Microarray Analysis: Pros and Cons
Chromosomal Microarray Analysis: Pros and Cons Since the 1960s, the traditional way of screening for prenatal genetic disorders was through amniocentesis, an invasive procedure, which slightly increases the risk of miscarriage. A new method of genetic testing, called chromosomal microarray analysis, can detect submicroscopic genetic anomalies that other tests can not find. In this method, fetal DNA is obtained through a sample of a pregnant woman's blood. This technology is believed to be safer, faster and more accurate than the traditional, invasive test. While there are many benefits to [more...]
Date: 2009-04-21 09:26:00
Role of p400/mDomino in Cell-cycle Progression [Dna and Chromosomes]
DNA microarray analysis revealed that the p400/mDomino deletion from MEFs caused the impaired expression of many cell-cycle-regulatory genes, including G2/M-specific genes targeted by the transcription factors FoxM1 and c-Myc. These results indicate that p400/mDomino plays a key role in cellular proliferation by controlling the expression of cell-cycle-regulatory genes. Footnotes ↵* This work was supported in part by grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan. ↵ The on-line version of this article (available at http://www.jbc.org) [more...]
Date: 2010-09-17 13:38:21
Arthritis Research & Therapy | Full text | Correction: DNA ...
Correction: DNA microarray analysis of rheumatoid arthritis susceptibility genes identified by genome-wide association studies Hidehiko Sugino1, Hooi-Ming Lee1 and Norihiro Nishimoto1,2 1 Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamada-Oka, Suita-City, Osaka 565-0871, Japan2 Laboratory of Immune Regulation, Wakayama Medical University, 105 Saito Bio Innovation Center, 7-7-20 Saito-Asagi, Ibaraki-city Osaka, 567-0085, Japan author email corresponding author emailArthritis Research & Therapy 2010, 12:403doi:10.1186/ar3023 See related letter by Sugino et al., [more...]
Date: 2010-05-24 00:00:00
Potential Bias in GO::TermFinder — Brief Bioinform
The increased need for multiple statistical comparisons under conditions of non-independence in bioinformatics applications, such as DNA microarray data analysis, has led to the development of alternatives to the conventional Bonferroni correction for adjusting P-values. The use of the false discovery rate (FDR), in particular, has grown considerably. However, the calculation of the FDR frequently depends on drawing random samples from a population, and inappropriate sampling will result in a bias in the calculated FDR. In this work, we demonstrate a bias due to incorrect random sampling in [more...]
Date: 2010-09-03 01:02:23
DNA saliva samples for SNP and CNV analysis on microarrays
Studies show that DNA from Oragene saliva samples gives equivalent results to DNA from blood for PCR, SNPgenotyping and microarrays. ... For any type of genetic analysis (e.g. population studies, clinical genetic testing, paternity testing, pharmacogenomic testing), non-invasive and easy-to-use sample collection techniques are preferred because they increase compliance rates and reduce costs. Oragene•DNA is a DNA self-collection kit that is completely non-invasive, [more...]
Date: 2010-10-05 15:34:00