Complete DNA Sequencing & Synthesis Services,DNA Sequencing Analysis System. Custom DNA Synthesis Quick Turnaround w/ 100% Accuracy Guaranteed Expression available. AltaVista found 18,300,000 results DNA replication DNA polymerases, isolated from cells, and artificial DNA primers are used to initiate DNA synthesis at known sequences in a template molecule. … How is dna synthesis made DNA polymerase is the enzyme that catalyzes the reactions involved in … After DNA synthesis is complete, the DNA is typically isolated and purified. … DNA and Protein Synthesis DNA and Protein [more...]
Date: 2010-06-13 03:43:08
BioPet DNA Breed Identification Kit | I Love Dogs Blog
Use DNA to discover the breeds present in your dog Painlessly collect your dog’s DNA sample and send it to BioPet for quick analysis Over 60 qualified breeds are on file Fast results via USPS within 2 weeks Even a prepaid return envelope for the DNA sample is included Product Description Advances in DNA testing technology mean that it has become possible to identify the ancestry of any dog by performing a simple test using a cheek swab. The DNA required for the test to be run is isolated from cells that are trapped on the DNA ID Card. All cells carry the same genetic material, regardless of [more...]
Date: 2009-12-18 14:44:21
Reveo's direct DNA sequencing using tunneling microscopy « The ...
Reveo is developing an ambitious technology to stretch out and deposit taut DNA on conductive surfaces for electronic base detection using one or more STM tips and tunneling current measurements. The linearization and deposition of nucleic acid sequences will likely be done using molecular combing. Reveo’s approach requires atomically flat and positively charged substrate surfaces (e.g. self-assembled monolayers on gold substrates or treated graphite substrates). In addition to molecular combing, Reveo has proposed other methods to linearize DNA, including electrophoretic and hydrodynamic [more...]
Date: 2010-08-25 17:49:12
In Cancer, One Ring To Rule Them All
Imagine that a machine draws your blood, screens it for genetic mutations and chemical variations that can cause cancer, and then pops out a drug tailor-made for your DNA. The hypothetical drug would target and fix the point irregularities which have accumulated over time that can lead to the formation of tumors — and cancer. "Personalized cancer drug delivery? Depending on the approach, it could be as soon as 10 to 15 years away," says Viterbi professor Andrea Armani , an assistant professor of the Mork Family Department of Chemical Engineering and Materials Science. Armani has received [more...]
Date: 2010-10-01 06:00:00
A GMO primer — What you should know about genetically modified ...
Ninety-five percent of the sugar-beet crop in the U.S. is genetically modified, but a recent federal ruling may have a huge impact on that. When it all began: The technologies that led to genetically modified foods were developed in the 1970s, with the advent of recombinant DNA technology. At the time, there were a lot of ethical debates and concerns about crossing the species barrier and transgenic technology. The focus then was on bacteria — mainly E. coli — and putting genes from different species into bacteria. Big Business: In the 1980s, Monsanto pushed to develop and use GM [more...]
Date: 2010-10-12 16:28:00
Graphene could revolutionize DNA sequencing - physicsworld.com
By feeding individual strands of DNA through nanometre-sized holes, researchers in the Netherlands say they have proved the principle of a revolutionary new DNA sequencing technique. The breakthrough is part of a worldwide race to develop fast and low-cost strategies to analyse these codes that underpin the chemistry of life. The genetic profile – or "genome" – of an organism is determined by recording the full sequence of acid base pairs that make up its DNA. In 2003, the Human Genome Project made history by determining the entire human genetic code – 3 billion DNA base pairs that took [more...]
Date: 2010-07-21 08:44:52
Research Technician (Cambridge MA) | PCR Jobs
Utilize molecular techniques, such as DNA isolation, PCR, and DNA hybridization assays, to determine bacterial associations in oral health and disease. See details at http://mim.forsyth.org. Required Education, Experience and Skills: Position requires a Bachelors degree with experience in molecular biology/microbiology. Good computer and organizational skills are required. The Forsyth Institute is located in close proximity of public transportation. Employee benefits include health and dental coverage, long-term disability, Forsyth contribution to a retirement plan, three weeks vacation, [more...]
Date: 2010-10-13 16:28:37
My Helical Tryst: Single Molecule DNA Sequencing
There are a number of companies that have recently launched competing technology platforms that are capable of sequencing DNA at the single molecule level. You may ask, why? Well, the answer is simply cost-effectiveness. DNA sequencing technology has been around for several decades, and to date, the Human Genome Project (HGP) is considered the pinnacle application of that technology. The cost of the HGP was approximately $3 billion and took about a decade and half. It was the molecular biology equivalent of putting a man on the moon. But what is the next step? Well, if we can systematically [more...]
Date: 2009-02-11 22:17:00
Genomic DNA extraction protocol protocols and methods
Research news, bioproducts, protocols, reagents, forums Genomic DNA extraction protocol High molecular weight genomic DNA extraction protocol: Buffer and reagent: Genomic DNA extraction buffer (250ml): 1M Tris.Cl (pH 8.0) 2.5ml 0.5M EDTA (pH 8.0) 50 ml Pancreatic RNase 5 mg 10% SDS 12.5 ml -------------------------------------------------- Adjust pH to 8.0 and adjust volume to 250ml with ddH2O Saturated phenol (pH 8.0) 10M ammonium acetate (NH4Ac) Protocol: 1. Weigh 0.5-1g fresh tissue and put in motar. Add liquid nitrogen to snap freeze tissue and blend tissue to [more...]
Date: 2006-08-29 00:00:00
Special offer -- free DNA isolation services from Oragene•DNA samples
DNA Genotek partner company, Precision Biomarker, is offering free DNA isolation services to Oragene DNA customers until Sept 30, 2010. ... Their services include: nucleic acid isolation, SNP genotyping, expression profiling, qPCR protocol design and processing, and custom bioinformatic analysis. Precision Biomarker Resources serves as a Genomics Service Provider for investigators working with DNA Genotek's Oragene•DNA sample collection system. [more...]
Date: 2010-07-27 13:37:00
Colony PCR: Another Use For Toothpicks « Promega Connections
Colony PCR: Another Use For Toothpicks July 26, 2010 by Isobel I still remember my JOY (sadly, yes) when I first learned about colony PCR. It allowed me to avoid a laborious procedure involving genomic DNA isolation, restriction digestion, and (the dreaded) Southern blotting. I was trying to show definitively that a transposon had successfully been inserted into a target gene. Using colony PCR, I could just amplify the DNA from a colony and then show that the transposon increased the size of the expected PCR product compared to a control. Joy. I could also use colony PCR to screen DNA [more...]
Date: 2010-07-26 15:13:31
TTC VIDEO – Major Transitions in Evolution
New technologies, such as DNA analysis, together with the latest fossil discoveries, make this a fast-changing subject that has grown far more detailed and complete than the presentation you may have learned in school. Among the scientific and popular misconceptions that have been overturned are these: * Neandertals: Recently sequenced DNA from Neandertals suggests that they were not an isolated throwback but contributed some genes to the modern human lineage. Genetic and other clues show that behaviorally Neandertals were very human-like. * Ardipithecus: The 2009 discovery of a relatively [more...]
Date: 2010-10-11 03:10:44
E. Coli Plasmid Vectors: Methods and Applications (Methods in ...
E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) (archive) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, N.J.), V. 235.) Table of Contents The Function and Organization of Plasmids Finbarr Hayes Choosing a Cloning Vector Andrew Preston Escherichia coli Host Strains Nicola Casali Chemical Transformation of E. coli W. Edward Swords Electroporation of E. coli Claire A. Woodall DNA Transfer by Bacterial Conjugation [more...]
Date: 2004-04-04 08:00:00
MO BIO Laboratories, Inc. DNA Isolation Products Used to Analyze ...
27 /PRNewswire/ — MO BIO Laboratories, Inc., the leader in soil, water and microbial nucleic acid purification, announced today that the company’s DNA isolation products were used to analyze DNA from microbes sampled from the oil plume created by the Deepwater Horizon explosion. The study published this week in the journal Science, examined the prevalence of hydrocarbon degrading genes in microbial …Further information: Article [more...]
Date: 2010-08-28 10:43:15
Tecan is first choice for quality and reliability
You are here: Home » News » Featured news » Tecan is first choice for quality and reliability Tecan is first choice for quality and reliability Publication date: 2 September 2010 Author: kdm communications limited Tagged with: Auto Q Biosciences, Kudrat Rahman, Regional Molecular Genetics Service (RMGS), Stuart Bayliss, Tecan The Regional Molecular Genetics Service (RMGS) at St Mary’s Hospital, Manchester, is using a Freedom EVO® liquid handling workstation in combination with chemagen’s proven nucleic isolation technology to perform fully automated DNA extraction. Supplied by [more...]
Date: 2010-09-02 16:21:24
Science and Nature DNA Extraction Kit « : Blisstree - Serious ...
Genetics and science are everywhere even at the London Toy Fair. I attended the Toy Fair today for PlayLibrary.com and came across the Science and Nature Australia booth. Before I found them, I had asked at the booth selling Science Museum kits whether they had any for forensic science. They didn’t and only had a chemistry set. How old school. Science and Nature, however, has several different science kits and toys. One is Biology Madness that includes instructions for 26 experiments and a DVD with five experiments filmed. The experiments cover many areas of science and include these about [more...]
Date: 2007-01-24 20:47:45
5 minute DNA Extraction in a Shot Glass
A blog that offers research to help you achieve and maintain health… "If we don't play God, who will?" – James Watson Intro: 5 minute DNA Extraction in a Shot Glass Despite its exotic-sounding name, DNA is ubiquitous – it can be found in every cell of every living thing and almost everywhere on the planet. Nonetheless, we rarely come face-to-face with the molecule itself – and it’s not because DNA is difficult to find or isolate! In this instructable, we’ll show you how to isolate your own DNA with little more than some dish soap, table salt, high-proof alcohol, a shot glass, and [more...]
Date: 2009-11-13 04:41:42
From iPSC to induced beta-cells, iN and iCM: dedifferentiation vs ...
96 Genomic DNA Isolation Mini Kit (Tissues/Cells), ABP-PP-JG090S $229.00, the best solution for for high through-put DNA isolation, first of a brand new product series offered from Allele. Promotion of the Week 081610-082210: last call for half price Premium Agarose for DNA or RNA gel. Place order today and secure the low price. 1. Zhou, Q., J. Brown, A. Kanarek, J. Rajagopal, and D.A. Melton, In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Nature, 2008. 455(7213): p. 627-32. 2. Vierbuchen, T., A. Ostermeier, Z.P. Pang, Y. Kokubu, T.C. Sudhof, and M. Wernig, Direct [more...]
Date: 2010-08-19 08:39:19
Genetic Fingerprinting of Bacillus thuringiensis Isolates by ...
Genetic Fingerprinting of Bacillus thuringiensis Isolates by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)(Published in Nepal Journal of Science and Technology 10(2009) 97-103)Gyan Sundar Sahukhall, Upendra Thapa Shrestha1, Binod Lekhak2, Anjana Singh2, Viswanath Prasad Agrawal11Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal. 2Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepale-mail: email@example.com AbstractRandom Amplified Polymorphic DNA (RAPD) is a method of producing a genetic fingerprint of a [more...]
Date: 2010-01-21 14:06:00
Plasmid isolation protocol — Isolation of DNA – miniprep
Be the first to comment - What do you think? Posted by admin - March 9, 2010 at 1:45 am Categories: Biology, Genetic, Protocols, Science, ppt files Tags: Dna, isolation, miniprep, Plasmid DNA, Plasmid isolation [more...]
Date: 2010-03-09 09:45:18
Noticias de Salud: Tecan is first choice for quality and reliability
Un blog con noticias de actualidad relacionadas con el mundo de la salud. Novedades, encuestas, estudios, informes, entrevistas. Con un sencillo lenguaje dirigido a todo el mundo. Y algunos consejos turísticos para pasarlo bien The Regional Molecular Genetics Service (RMGS) at St Mary’s Hospital, Manchester, is using a Freedom EVO® liquid handling workstation in combination with chemagen’s proven nucleic isolation technology to perform fully automated DNA extraction. Supplied by chemagen’s UK distributor, Auto Q Biosciences, this integrated system is housed within a Class 1 biological [more...]
Date: 2010-09-02 16:25:00
GATE 2011 Syllabus for Biotechnology | GATE 2011 Biotechnology ...
Recombinant DNA Technology: Restriction and modification enzymes; Vectors: plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; cDNA and genomic DNA library; Gene isolation; Gene cloning; Expression of cloned gene; Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain reactions; DNA fingerprinting; Southern and northern blotting; In-situ hybridization; RAPD; RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy.w w w.w a y 2 f r e s h e r s . c o m Bioinformatics: Major bioinformatics resources (NCBI, [more...]
Date: 2010-09-01 10:17:23
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are involved in various disease problems in humans and aquatic animals and are known to be phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific diagnostic test is warranted for detection and characterization of this pathogen.In the present study, Fish and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]
Date: 2010-10-08 20:08:05
Evaluation of Five Methods for Total DNA Extraction from Western ...
DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. Methodology/Principal Findings From individual western corn rootworm beetles, Diabrotica virgifera virgifera, DNA extractions by the SDS method, CTAB method, DNAzol® reagent, Puregene® solutions and DNeasy® column were compared in terms of DNA quantity and quality, cost of materials, [more...]
Date: 2010-08-13 07:00:00
Low Satellite DNA Variability in Natural Populations of Drosophila ...
Low Satellite DNA Variability in Natural Populations of Drosophila antonietae Involved in Different Evolutionary Events Fernando Faria Franco, Fabio Melo Sene and Maura Helena Manfrin From the Campus de Sorocaba, Universidade Federal de São Carlos, CEP 18052-780, Sorocaba, Brazil (Franco); the Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil (Sene and Manfrin); and the Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil (Sene and [more...]
Date: 2010-05-24 09:27:37
AIR™ DNA Sample Prep Kits Now Available for Illumina-Compatible ...
Bioo Scientific has expanded its line of kits for next generation sequencing sample preparation with the introduction of the AIR Genomic and AIR Paired-End DNA Sequencing Kits, designed for use with the llumina GAII and HiSeq 2000 sequencers. These kits offer flexible, cost-effective, single day sample preparation protocols. It has become obvious that the use of the same preparatory methods to identify and quantify rare sequences and perform large scale comparative evolutionary studies is not ideal and that there are clear advantages for particular applications over others. Bioo [more...]
Date: 2010-10-05 18:12:01
Intellectual Property Expert Group (ipeg) » Blog Archive » Are the ...
These are just two recent cases in the US but the implication on DNA sequence patents may be far reaching. Back in 2000, when the new economy was still alive, there was a lot of focus on DNA sequence patents. A lot of companies were very cautious and a lot of genetic test developments were stalled because of patent issues. When asking an expert you could get philosophical answers: either you licence or you ignore or you litigate. Now in 2000 there were still patents from the early days of DNA sequencing around. Some of these patents might have been inventive as the inventive step was a lot [more...]
Date: 2010-09-20 16:55:26
Hisar Practical Training Course Recombinant DNA Techniques ...
Date: 2010-10-05 04:07:03
Plasmid DNA Isolation
Everything that you every wanted to know about Plasmid DNA Isolation, and trust me when I say EVERYTHING!!! Comment by Qr1Zz 2010-07-3 20:44:59 omg.. no glows, drinking in the lab, always working while your sitting on your chair and not wearing a lab coat, what kind of hobby scientists are [more...]
Date: 2010-07-03 20:44:59
Yonder Biology Kicks Off World Cup Fever with Custom DNA Art
Yonder Biology, a DNA Art Company, is offering Custom World Cup DNA Art Images. Just name your favorite country when you purchase a DNA Art Portrait from Yonder Biology and Yonder will combine the two – your DNA image and your country’s colors- to create a one of kind DNA Art. Soccer – Is it in your DNA? Each unique DNA image will be printed on canvas, photo paper or as a premium Face Mount image. How Spanish are you… really? Yonder Biology DNA Art Portraits can be viewed at www.yonderbiology.com. How to purchase your very own DNA Portrait: The process begins with a visit to the [more...]
Date: 2010-07-10 04:01:00
CIENCIASMEDICASNEWS: Recurrent Granulibacter bethesdensis ...
DNA was isolated from human tissue by using the Maxwell 16 Tissue DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. DNA concentrations were measured by using a UV spectrophotometer (NanoDrop, Wilmington, DE, USA). The 16S rRNA and methanol dehydrogenase subunit 1 (GeneID YP_744165.1) genes of G. bethesdensis were analyzed by using a PCR and primer sequences 16S-forward: 5'-TCGGGTGGGCACTCTAAAGG-3', 16S-reverse: 5'-GCATCACTGCCTAGCTTCCC-3', MDH-forward: 5'-CCGCAATACGGTCAATTCG-3', and MDH-reverse: 5'-GCCGATCTTCCAGGTTTCTTC-3'. Each reaction mixture [more...]
Date: 2010-09-02 23:42:00
fenian32: Colin Duffy sexually violated in prison search, court told
The court was told that DNA samples taken from a seatbelt, in what is believed to have been the getaway car, and the tip of a latex glove that was found in the vehicle, match DNA samples taken from Duffy and therefore link him to the car. An apparent attempt to burn the car, which had been bought 13 days before the attack through Autotrader for £380, had failed. A forensic scientist told the court that the chance of the DNA found on the latex glove belonging to any other person but Duffy was one in a billion. The probe also heard that DNA on a mobile phone and two matchsticks found inside [more...]
Date: 2010-10-13 15:58:36
Plasmid DNA isolation solution III - 10760030-1 : PlantMedia ... Plasmid DNA Isolation, Solution III Common buffer used for DNA isolation Technical Information: 5M Potassium Acetate (60ml) Glacial Acetic Acid (11.5ml) H2O (28.5ml) [more...]
Date: 2010-10-04 12:58:18
MOBIO Laboratories Releases UltraClean Microbial DNA Isolation Kit ...
Cookie cutter DNA extraction kits simply won't break through tough microbial samples. UltraClean® Microbial DNA Isolation Kit from MO BIO Laboratories, is specifically designed to quickly and safely extract high quality DNA from a wide [more...]
Date: 2010-06-28 13:38:07
Genetic ancestry has no effect on asthma response in African ...
Their breathing function was tested before and after the six-week treatment protocol. Genetic testing was done on DNA isolated from a single blood sample. “This is one of the first studies to use genetic ancestry to help clarify whether response to medication differs from race or ethnic groups. Fortunately, our results suggest that this potentially life-saving asthma medication is equally beneficial across groups,” said Dr. Keoki Williams, the study’s senior author. The study is published online at the Journal of Allergy and Clinical Immunology. (ANI) Posted by Tanushree on Sep 28th, [more...]
Date: 2010-09-28 10:02:07
plasmid dna isolation
The DNA of prokaryotic cells (bacteria) is relatively simple in comparison to the DNA of eukaryotic cells. Possibly Related Posts: 1994 INAC Seaweed collection expedition to the Antarctic Peninsula Epigenetics Webinar (Part 2 of 4) As a sequence of the genome sequencing of 7 Products Sounds Seen # 1 – 1 opponent Bioluminescent [more...]
Date: 2010-06-21 04:08:42
Cytoplasmic & Nuclear RNA Purification Kit
RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]
Date: 2010-08-16 04:58:00
Extraction of DNA from Fish Fins (Amrita University)
Many different methods and technologies are available for the isolation of genomic DNA. Here, we describe a method for the isolation of PCR-ready genomic DNA from zebra-fish tissues. Video Rating: 0 / 5Source: DNA [more...]
Date: 2010-10-09 19:26:00
I will start to do DNA experiment on Indonesian local jelly fish ...
I will start to do DNA experiment on Indonesian local jelly fish (including DNA and protein extraction)? Question Is there any suggestion what is the key point/step for DNA and protein isolation and purification protocols from jellyfish? Please suggest some good references, too. Thank you Answer Well. This isn't a thing that you should ask on Yahoo! answers. I think that you should ask somebody which really knows what he's talking about, especially if it is a practical experiment and not just a curiosity. As the other guy said you should see a professor from the [more...]
Date: 2010-09-21 14:23:26
A robust universal method for extraction of genomic DNA from ...
The intactness of DNA is the keystone of genome-based clinical investigations, where rapid molecular detection of life-threatening bacteria is largely dependent on the isolation of high-quality DNA. Various protocols have been so far developed for genomic DNA isolation from bacteria, most of which have been claimed to be reproducible with relatively good yields of high-quality DNA. Nonetheless, they are not fully applicable to various types of bacteria, their processing cost is relatively high, and some toxic reagents are used. The routine protocols for DNA extraction appear to be [more...]
Date: 2010-08-21 07:44:00
Is there a different type of primer i have to use when running PCR ...
Question by bob oso: Is there a different type of primer i have to use when running PCR with genomic DNA compared to cDNA? Im still in school but when i run a PCR, I normally isolate RNA first then RT to cDNA and run PCR with that. This time i isolated the genomic DNA from the sample and ran PCR using the beta-actin primer i always used. Dimers were faintly visible on my gel but nothing really came up like it normally would have or should have. I was told that when running PCR with genomic DNA, a different kind of primer had to be used; one that is specific to genomic DNA. Im confused as to [more...]
Date: 2010-10-01 19:54:46
Paternity Blood Test | Diet and Health Tips & Products Reviews
Today, the most accurate tests (99.99%) to determine the paternity of a child are made through DNA. Collecting DNA sample is done two ways: Buccal swab (swabbing the inside of the cheek) and blood samples. Known as PCR (polymerase chain reaction), the procedure is used in comparing samples of the childís DNA with that of the alleged father. Since the childís DNA is a fairly equal combination of DNA of his parents, excluding the DNA of the mother gives us a genetic profile of the biological father. However, before science knew how to isolate DNA, blood samples were the standard in a variety [more...]
Date: 2010-04-29 03:30:53
Plasmid DNA Extraction Kit
DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the environment as well as diagnosing disease and genetic disorders. The GenQuik Plasmid DNA Extraction Kit, utilizing an exclusive membrane technology in the form of convenient spin columns and 96 well plates, makes the isolation of high quality plasmid DNA from recombinant E.coli cultures simple, rapid and cost-effective. For Details on GenQuick Plasmid DNA [more...]
Date: 2010-07-29 17:42:05
Feedback from Interpol DNA User's Conference | DNA Project - South ...
This time last week I was sitting at the Interpol Headquarters in Lyon, France listening to the Chief of the USA FBI CODIS Unit talk about International DNA Exchange methods. This was only one of approximately 35 fascinating presentations delivered by representatives from over 50 countries. As you may well imagine, participating in a conference of this magnitude was extraordinary, and the value of being able to interact with the other representatives: priceless. A special mention of thanks needs to go to our generous sponsor, The Open Society Foundation for South Africa, which enabled us to [more...]
Date: 2010-09-29 10:51:03
Kuwait Institute Develops Alternative to Antibiotics
He said the team will proceed to the next process of testing and evaluating these isolated strains using DNA sequencing technology. ThePoultrySite News Desk. Sign up for free poultry news updates. Sign up for our ... 5M Publishing. Learn how to market your products here. 5M Enterprises Ltd., Benchmark House, 8 Smithy Wood Drive, Sheffield, S35 1QN, England. 5M Enterprises Inc., Suite 4120, CBoT, 141 West Jackson Boulevard, Chicago, IL, 60604-2900, USA. [more...]
Date: 2010-09-22 23:00:00
Protocol for plasmid dna isolation
Be the first to comment - What do you think? Posted by admin - March 9, 2010 at 12:19 am Categories: Biology, Genetic, Protocols, ppt files Tags: Dna, isolation, plasmid, powerpoint presentation, ppt, [more...]
Date: 2010-03-09 08:19:37
Gerard Biotech Spin Doctor Genomic DNA isolation kits
**From Tails to PCR in under 2 hours!** +Individual Lypilized tubes can digest samples for PCR in 1-2 hours +15 minute post digest cleanup for +PCR, 30 minute digest for Southerns May be stored safely at room temperature, no need to freeze or aliquot **No column, binding resin, or phenol** +Great yeilds from small samples; 1 mm tail snips, ear punches, young mice +Gentle process will not shear DNA +Eliminates the need for hazardous organic solvents [more...]
Date: 2010-09-29 10:13:26
Plant RNA/DNA Purification Kit
Total DNA and RNA from 50 mg of tobacco, tomato and peach were simultaneously isolated with Bio-Synthesis's Plant RNA/DNA Purification Kit (with the optional on-column RNase treatment). Next, 2µL of DNA from each 150 µL elution was mixed in 20 µL of PCR reaction and used in a real-time PCR (95ºC for 3min and 40 cycles at 95 ºC for 15 sec. and 60 ºC for 30 sec.). DNA from tobacco (green), tomato (red) and peach (blue) was successfully amplified, indicating the high quality of the DNA for downstream application. Primer dimer (black) also was shown. Figure 3. Isolate High Quality Total RNA [more...]
Date: 2010-08-16 04:44:00
Troubleshooting RNA Isolation | Bitesize Bio
Cause: No matter what method you use for RNA isolation, traces of DNA always carry through. This is true with TRIzol (phenol) preps and with silica spin filters. This can be caused by insufficient shearing of the genomic DNA during [more...]
Date: 2010-05-12 15:19:56
#extraction of plasmid dna - DNA07.pdf by reading.ac.uk - #pdf ...
This is the classical' method of isolating plasmid DNA; with it you can see the ... suppliers of materials for molecular biology, but these tend to be too costly ... Filename : DNA07.pdf Publisher : ncbe.reading.ac.uk [more...]
Date: 2010-07-27 05:22:33