MOBIO Laboratories Releases UltraClean Microbial DNA Isolation Kit ...
Cookie cutter DNA extraction kits simply won't break through tough microbial samples. UltraClean® Microbial DNA Isolation Kit from MO BIO Laboratories, is specifically designed to quickly and safely extract high quality DNA from a wide [more...]
Date: 2010-06-28 13:38:07
Efavirenz Plasma Concentrations and Cytochrome 2B6 Polymorphisms ...
Genomic DNA was extracted from the peripheral whole blood of each subject with a DNA extraction kit (Qiagen, Valencia, CA). CYP2B6 polymorphisms were genotyped by resequencing using primers and polymerase chain reaction (PCR) conditions described by Lamba and associates.15 Sequencing was carried out with an ABI Prism 3700 Automated Sequencer using the PCR primers or internal primers (sequence available on request). Sequences were assembled using the Phred-Phrap-Consed [more...]
Date: 2010-09-22 19:28:38
Virology Journal | Full text | Detection of hepatitis B virus DNA ...
Compared with the final volume of 40 μl DNA extracted from 20 μl plasmas according to the instruction of the real-time PCR kit, the DNA extraction steps in this study actually concentrated the plasma DNA with 20-fold. Thus, by reverting the concentrated DNA to the original level, the actual viral loads in these two plasmas were approximately 105, 110, and 600 copies/ml respectively (Table [more...]
Date: 2010-08-19 00:00:00
Effectiveness of DNA Extraction Protocols for Horticultural and ...
This study analyses the effectiveness of 12 previously published DNA extraction protocols used for the extraction of DNA in various species in contrast to 3 commercially available plant DNA extraction kits. In addition, an innovative, [more...]
Date: 2007-08-01 16:17:28
article - Aspergillus DNA contamination in blood collection tubes
manufacturers using a DNA extraction kit that has been proven free from fungal DNA (data not shown) (MycXtra;. Myconostica, Manchester, United Kingdom). Extracted. DNA was tested using a quality controlled commercial [more...]
Date: 2010-08-06 20:26:58
An evaluation of commercial DNA extraction kits for the isolation ...
Significance and Impact of the Study: The results of this study aid in the selection of an appropriate DNA extraction kit for a given soil sample. Its application could expedite sample processing for real-time PCR detection of a [more...]
Date: 2010-08-01 09:43:21
Consumer Reports Health Blog: Should you screen your genes at ...
UPDATE: Jim Cohn, a Walgreens spokesman, emailed us this statement this morning: "In light of the FDA contacting Pathway Genomics about its genetic test kit and anticipated ongoing discussions between the two parties, we’ve elected not to move forward with offering the Pathway product to our customers until we have further clarity on this matter." Absolutely about time. Dr's have hidden things and used thier control long enough. We have a right to know, even if it is flawed, what makes us who we are. Long ago, the idea that the earth was round was considered dangerous. Then it simply [more...]
Date: 2010-05-12 21:10:58
HTB – Early infant diagnosis
She concluded that promising POC assays for EID today include: IQuum’s LIAT, SAMBA, CIGHT p24 and possibly CLONDIG’s viral load assay. comment This was an incredibly useful overview and it looks like we can be optimistic about having a POC test for EID in the next couple of years. Other presentations at the conference dealt with the challenges of access to EID. In the same session, Shaffiq Essajee noted that EID access has improved and in some countries more than 50% of exposed infants are tested. But infant testing is usually linked to PMTCT and if coverage is low, so is infant [more...]
Date: 2010-09-30 06:04:59
Genome Biology | Full text | Genomic acquisition of a capsular ...
Bacterial strains and genomic DNA extraction. Bacterial cultures were grown in LB media and genomic DNA was extracted using the Qiagen Genomic DNA kit (Qiagen, Hilden, Germany). For morphological characterization, strains were cultured [more...]
Date: 2010-08-27 00:00:00
Plasmid DNA Extraction Kit
DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the environment as well as diagnosing disease and genetic disorders. The GenQuik Plasmid DNA Extraction Kit, utilizing an exclusive membrane technology in the form of convenient spin columns and 96 well plates, makes the isolation of high quality plasmid DNA from recombinant E.coli cultures simple, rapid and cost-effective. For Details on GenQuick Plasmid DNA [more...]
Date: 2010-07-29 17:42:05
DNA Gel Extraction Kit
The DNA Gel Extraction Kit is designed for the rapid purification of DNA fragments from agarose gels. The kit allows for the removal of agarose, enzymes, dNTPs, ethidium bromide, dyes and detergents from the DNA fragment. The recovered DNA is of a high quality, and is suitable for a number of downstream applications including restriction enzyme digestions, ligations, labeling, hybridizations and sequencing. The kit employs the use of a proprietary resin for DNA purification. This resin is an extremely inert and stable substance and is able to preferentially bind nucleic acids in the presence [more...]
Date: 2010-03-05 04:40:00
IVF Mail - single cell DNA Extraction
Hi all.. please can you tell me how we can extract DNA from single cell for downstream amplification using real time PCR (PGD analysis). please if you know tell me the name of such kit used and the company manufacture it. really I am very interested in this [more...]
Date: 2010-10-04 22:33:19
"Happy and Healthy" A Cancer Free Living Blog: Bone Marrow ...
Go to http://www.marrow.org/, fill out the online registration form and get your at home registration kit within a few weeks. Once you receive the kit, follow the simple directions and send back your DNA sample. The sample will then be analyzed and the results are compared to potential recipients. Like myself you may of heard "bone marrow transplant" and had visions of long needles and painful procedures, but getting registered is nothing more than a cheek swab. That's right, a cotton swab to the inside of the cheek. Nothing more than what you see for a DNA test on TV. If you're a match for [more...]
Date: 2010-10-12 19:55:00
ScienceWiz DNA Kit
Contains 172 pieces to build a double helix, graduated test tube with caps, thermometer, pipettes, filter paper, 47 chromosome puzzle pieces, a chromosome tray, eppendorf tubes, DNA extraction buffers, ink base, in well, ink quill and a [more...]
Date: 2010-07-02 13:03:54
Preps in the Zyppy: How I Changed my DNA Miniprep, Gel Extraction ...
Breaking up is never easy, especially when you've been together a long time. But when you meet the right one it's so much easier. Here's how I ditched my old miniprep kit for something [more...]
Date: 2008-01-21 09:47:18
gel extraction protocols - SEQanswers
Hi, I'm hoping to collect some information on how you all handle the gel extraction step in the Illumina sample prep protocol. I have had some issues getting a decent yield of DNA out of 2% TAE agarose gels with the Qiagen QiaexII kit. What kits do you find work particularly well? Any tips for sterile gel prep, visualization, and band excision? Does EtBr cause downstream problems? Does anyone use the new(ish) high voltage electrophoresis buffers (Na-borate/Li-borate/Li-acetate) and/or visual stains (crystal violet/methylene [more...]
Date: 2009-05-27 00:26:23
New extraction kit for Oragene•DNA available from Beckman Coulter
DNAdvance SP Delivers High Yield with Oragene DNA [more...]
Date: 2010-07-13 14:32:00
Quick Kit for Bacterial Total DNA Isolation
This kit is a very quick and easy one for bacterial total DNA (chromosome and plasmid) isolation with fewer steps. It takes less than 15 min. for sample to be ready for loading on agarose gel electrophoresis. Using this kit, the plasmid takes only one from its three forms after isolation, which a appears as one clear and sharp band in the agarose gel after electrophoresis (in addition to chromosomal band). Making the detection of plasmid in agarose is much easier and also determined, if the sample has more than one plasmid. This kit is able to isolate total DNA from bacteria cell with high [more...]
Date: 2010-10-05 18:29:32
Evaluation of Five Methods for Total DNA Extraction from Western ...
DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. Methodology/Principal Findings From individual western corn rootworm beetles, Diabrotica virgifera virgifera, DNA extractions by the SDS method, CTAB method, DNAzol® reagent, Puregene® solutions and DNeasy® column were compared in terms of DNA quantity and quality, cost of materials, [more...]
Date: 2010-08-13 07:00:00
Science and Nature DNA Extraction Kit « : Blisstree - Serious ...
Genetics and science are everywhere even at the London Toy Fair. I attended the Toy Fair today for PlayLibrary.com and came across the Science and Nature Australia booth. Before I found them, I had asked at the booth selling Science Museum kits whether they had any for forensic science. They didn’t and only had a chemistry set. How old school. Science and Nature, however, has several different science kits and toys. One is Biology Madness that includes instructions for 26 experiments and a DVD with five experiments filmed. The experiments cover many areas of science and include these about [more...]
Date: 2007-01-24 20:47:45
Company Profile : Diagnostic supplier : Suyog Diagnostics : Mumbai ...
The company is representing world renowned Diagnostics Companies and selling Diagnostics Kits for Coagulation disorders, Viral Markers for HIV, HBV, HCV, HDV, HEV, Biochemistry, Chromatography kits etc. Life Science Division: The company is representing world renowned Diagnostics Companies and selling Chemicals, Bio - Chemicals, FISH Probes, Radio Chemicals, Reagents, Cell Bio, Electrophoresis grade, Neuro chemicals, Molecular Biology grade, DNA Extraction kits, PCR Kits, Restriction Enzymes, DNA Ladders & modifying enzymes, Proteomics, Cell Culture reagents, Plant Tissue Culture Grade [more...]
Date: 2010-07-09 15:29:00
Plasmid DNA extraction from e.coli for digestion mapping clony ...
Research news, bioproducts, protocols, reagents, forums Plasmid DNA extraction from e.coli for digestion mapping clony screening Plasmid DNA extraction from e.coli for digestion mapping clony screening: This protocol utilize the Qiagen buffer P1, P2 and P3 from Qiagen plasmid purification kit. RNase must be added before use. It is cost efficient since it doesn't use expensive plasmid purification columns. This protocol is only intended for screening positive clony. Once positive clonies are determined, purification from bacterial overnight culture can be done using plasmid [more...]
Date: 2006-08-30 07:00:00
Eppendorf to market Akonni TruTip™ « Frederick County Biotech ...
HAUPPAUGE, N.Y. & FREDERICK, Md.–(BUSINESS WIRE)–Eppendorf North America and Akonni Biosystems today announced they have entered into a joint marketing agreement to promote Akonni TruTip nucleic acid extraction kits configured for use with Eppendorf epMotion automated pipetting systems. Under the agreement, Eppendorf will promote the extraction kits to clinical, clinical research and forensic laboratories in North America – providing users with access to the industry’s most rapid and reliable means to automatically extract PCR-ready DNA and/or RNA from larger volume [more...]
Date: 2010-04-26 16:36:03
Genomic DNA Extraction » DNA Extraction Protocol Video
Video showing how DNA Extraction protocol should be conducted. It shows how we can extract dna from different organisms. If you want to learn about Genomic DNA Extraction and more about DNA Purifcation you should watch this video. All you need is DNA Extraction Kit. In School or College Lab this DNA Kit is ussually easy to find. Filed under: Genomic DNA Extraction by [more...]
Date: 2010-03-04 08:17:21
Research Progress Report I
Without the qPCR results, it is hard to say which clean-up procedure is better, but it seems that both have about the same yield of DNA. There was not enough DNA in the PC+Q sample to run a qPCR. Another thing to consider is that this experiment was performed with a Qiagen gel extraction kit, not a kit designed specifically for DNA clean-up. Using such a kit (they do have one) may possibly yield better results. Future Experiments: I am currently growing up 250mL worth of AFT1 cells for my next ChIP experiment. I will probably be comparing the old bead beater with the new bead [more...]
Date: 2010-10-04 01:35:29
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
Plasmid DNA Extraction Kit
DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the [more...]
Date: 2010-08-04 07:44:57