GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are involved in various disease problems in humans and aquatic animals and are known to be phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific diagnostic test is warranted for detection and characterization of this pathogen.In the present study, Fish and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]
Date: 2010-10-08 20:08:05
Plasmid DNA Extraction Kit
DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the [more...]
Date: 2010-08-04 07:44:57
A robust universal method for extraction of genomic DNA from ...
The intactness of DNA is the keystone of genome-based clinical investigations, where rapid molecular detection of life-threatening bacteria is largely dependent on the isolation of high-quality DNA. Various protocols have been so far developed for genomic DNA isolation from bacteria, most of which have been claimed to be reproducible with relatively good yields of high-quality DNA. Nonetheless, they are not fully applicable to various types of bacteria, their processing cost is relatively high, and some toxic reagents are used. The routine protocols for DNA extraction appear to be [more...]
Date: 2010-08-21 07:44:00
If You Were Able To Extract Dna From The Bacteria Using The Same ...
would you expect a yield of DNA to be the same, more or less? explain. Related questions I’m Looking For Some Opinions On This Piece Of Writing.? (1) I’m Looking For Some Opinions On This Piece Of Writing.? (1) Don’t Know What To Do, My Mother In Law Actually Is Having A Psychotic Break.? (4) Don’t Know What To Do, My Mother In Law Actually Is Having A Psychotic Break.? (4) Would I Ever Find A Girl Like Me? My Perfect Match? (12) First of all the answer from jai is bogus. He is talking about transformation, not DNA extraction (sorry jai). As for your question, jeez it just depends [more...]
Date: 2010-09-29 22:15:19
Clue to pregnant blood pressure - NHS Choices - Your health, your ...
The researchers created bacteria that contain the DNA sequence for making mouse, rat or human angiotensinogen. In this way they could use the bacteria to produce lots of the angiotensinogen protein, which could then be extracted and purified. ... While the results of this research are compelling, raised blood pressure is only one initial symptom of pre-eclampsia. Pre-eclampsia is also characterised by other symptoms, namely protein in the urine and fluid retention. [more...]
Date: 2010-10-07 17:15:00
O.N.L.Y 1 %: 10 Tips For Better DNA Gel Extraction Results
Nabi s.a.w bersabda, maksudnya: Jabir mengatakan bahawa Rasulullah s.a.w pernah bersabda, "Wahai manusia, aku telah meninggalkan untuk kalian 2 perkara. Jika kalian menggunakannya tidak akan tersesat selamanya iaitu Al-Quran dan Ahlul Bait keturunanku." (HR At-Tarmizi dan ia menghasankannya) What is it about gel extraction of DNA that makes it a pain? Maybe it’s poor product yields or maybe it’s because the process uses harsh chemicals (chaotropic salts, ethidium bromide, ethanol, heat) that will damage or denature DNA and potentially decrease cloning success. In this article I share some [more...]
Date: 2010-10-11 04:17:00
see the world from science lens: ScienceDaily Environment ...
Yersinia pestis bacteria confirmed as cause of Middle Ages 'Black Death' plague epidemic (October 8, 2010) -- The latest tests conducted by anthropologists in Germany have proven that the bacteria Yersinia pestis was indeed the causative agent behind the "Black Death" that raged across Europe in the Middle Ages. ... > full story Scientists trick bacteria into embedding small molecules in cell wall (October 8, 2010) -- Scientists have engineered the cell wall of the Staphylococcus aureus bacteria, tricking it into incorporating foreign small molecules and embedding them within the cell wall. [more...]
Date: 2010-10-11 09:21:00
The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...
There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]
Date: 2010-10-04 21:15:46
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
University researchers working to perfect micro DNA technology ...
University of Virginia researchers are working to develop “lab-on-a-chip,” which could drastically change such scenarios by shrinking technology that requires time, laboratories full of people and expensive equipment into a glass slide smaller than a credit card. Such technology has been promised for close to two decades, warns James Landers, a University of Virginia professor of chemistry and mechanical engineering and an associate professor of pathology. He is the lead researcher for the lab- on-a-chip project. While he is cautious about the “unknowns” yet to be dealt with, Landers [more...]
Date: 2008-11-14 05:01:00
Rapid Boiling Method for Plasmid DNA Extraction
In the previous post, it had already explained about how to extract plasmid DNA by using alkaline lysis method. In this particular post, it will be explained the other method to extract plasmid DNA instead of using alkaline lysis method. It is Rapid Boiling method, an alternative to alkaline lysis method that was developed by Holmes and Quigley. Here, the cells are lysed partially allowing plasmids to escape, whereas the bacterial chromosomal DNA remains trapped in the cell debris. High temperature is then used to denature the chromosomal DNA, after which reannealing allows the plasmids to [more...]
Date: 2009-09-01 07:00:00
Plasmid DNA Extraction Using Alkaline Lysis Method
At this point bacterial chromosomal DNA is usually seen as a white precipitate. Centrifuge the tubes for 2-5 min at full speed in a microfuge. Place new sterile tubes into a rack, label them, and add 250 ~ of isopropanol to each tube. Remove the tubes from the microfuge, being careful not to disturb the precipitate. Remove the supernatant with a 1-mL pipet, avoiding the white precipitate as much as possible. Transfer the liquid phase into the new set of labeled tubes containing the isopropanol. Vortex the tubes for 5-10 s and centrifuge the tubes in the microfuge for 30 s at high speed. The [more...]
Date: 2008-08-11 07:00:00
Why is the germ ideal for DNA extraction? (in a wheat seed)?
A seed is basically just an egg. The “germ” of a grain is the actual embryo within the seed, while the majority of the rest of the seed is fuel that the new plant will use for its initial growth, and is basically little more than starches and sugars. In short, ALL the DNA in the seed is in the [more...]
Date: 2010-04-16 02:58:01
Molecular biology Practical
For Latest Career Opportunities in Biotechnology and Life Sciences | Please visit | Navin K BIOTECH | India's No. 1 Biotechnology and Life Sciences Career Blog | log on to [more...]
Date: 2010-06-29 19:47:00
Plasmid DNA Extraction Kit
DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the environment as well as diagnosing disease and genetic disorders. The GenQuik Plasmid DNA Extraction Kit, utilizing an exclusive membrane technology in the form of convenient spin columns and 96 well plates, makes the isolation of high quality plasmid DNA from recombinant E.coli cultures simple, rapid and cost-effective. For Details on GenQuick Plasmid DNA [more...]
Date: 2010-07-29 17:42:05
Changes in the rumen bacterial community in response to sunflower ...
After 0, 3, and 10 d, rumen content samples were taken for DNA extraction and FISH analysis (fluid). Total bacteria and the Butyrivibrio group were studied in microbial DNA by terminal RFLP analysis (T-RFLP), and real-time PCR was used to quantify Butyrivibrio bacteria that produce vaccenic acid or stearic acid. In rumen fluid samples, total bacteria and clostridial clusters IX and XIV were analyzed by FISH. Dietary supplementation with SO plus FO seemed to induce important changes in the total bacteria and Butyrivibrio populations, and a high interindividual variation was observed, and the [more...]
Date: 2010-08-13 16:00:51
Top ten most accessed articles in August
Cell lysis and DNA extraction of gram-positive and gram-negative bacteria from whole blood in a disposable microfluidic chip Madhumita Mahalanabis, Hussam Al-Muayad, M. Dominika Kulinski, Dave Altman and Catherine M. Klapperich Lab Chip, 2009, 9, 2811 – 2817, DOI: 10.1039/b905065p Programmable diagnostic devices made from paper and tape Andres W. Martinez, Scott T. Phillips, Zhihong Nie, Chao-Min Cheng, Emanuel Carrilho, Benjamin J. Wiley and George M. Whitesides Lab Chip, 2010, 10, 2499 – 2504, DOI: 10.1039/c0lc00021c Microfluidics without pumps: reinventing the [more...]
Date: 2010-09-28 08:45:53