AcroMetrix Announces the Launch of Calibration Panel for CMV DNA ...
The panel was designed to provide traceable results and possess commutable properties across assay systems. A material is considered "commutable" if it behaves like a patient sample on different diagnostic platforms. The OptiQuant CMVtc Calibration Panel is intended for use as a standard to assess the performance of molecular diagnostic test procedures for the determination of human cytomegalovirus (CMV) DNA. Accurate diagnosis of patient disease states requires reference materials that are not only traceable to a higher-order standard, but also closely mimic true patient samples, a property [more...]
Date: 2010-10-10 09:15:40
Research Technician (Cambridge MA) | PCR Jobs
Utilize molecular techniques, such as DNA isolation, PCR, and DNA hybridization assays, to determine bacterial associations in oral health and disease. See details at http://mim.forsyth.org. Required Education, Experience and Skills: Position requires a Bachelors degree with experience in molecular biology/microbiology. Good computer and organizational skills are required. The Forsyth Institute is located in close proximity of public transportation. Employee benefits include health and dental coverage, long-term disability, Forsyth contribution to a retirement plan, three weeks vacation, [more...]
Date: 2010-10-13 16:28:37
Electrophoresis: Agarose Gel Electrophoresis, Gel Electrophoresis ...
Purchase includes free access to book updates online and a free trial membership in the publisher’s book club where you can select from more than a million books without charge. Chapters: Agarose Gel Electrophoresis, Gel Electrophoresis, Sds-Page, Capillary Electrophoresis, Terminal Restriction Fragment Length Polymorphism, Temperature Gradient Gel Electrophoresis, Two-Dimensional Gel Electrophoresis, Dielectrophoresis, Comet Assay, Electrophoresis, Qpnc-Page, Serum Protein Electrophoresis, Electrophoretic Mobility Shift Assay, Dna Separation by Silica Adsorption, Immunoelectrophoresis, [more...]
Date: 2010-07-30 11:54:06
Complete DNA Sequencing & Synthesis Services,DNA Sequencing Analysis System. Custom DNA Synthesis Quick Turnaround w/ 100% Accuracy Guaranteed Expression available. AltaVista found 18,300,000 results DNA replication DNA polymerases, isolated from cells, and artificial DNA primers are used to initiate DNA synthesis at known sequences in a template molecule. … How is dna synthesis made DNA polymerase is the enzyme that catalyzes the reactions involved in … After DNA synthesis is complete, the DNA is typically isolated and purified. … DNA and Protein Synthesis DNA and Protein [more...]
Date: 2010-06-13 03:43:08
Dienekes' Anthropology Blog: mtDNA of Yemeni and Ethiopian Jews
The way I see it, only a large-scale study of all global Jewish populations may uncover verified ancient Jewish lineages for both Y-chromosomes and mtDNA. The recent studies on Jews have uncovered several genetic sub-clusters of Jews, and only lineages that occur in 2 or more of these clusters, and preferably geographically separated ones have a strong claim of representing original Jewish lineages. There is a limit on what can be uncovered about the past from the study of living populations. American Journal of Physical Anthropology doi: 10.1002/ajpa.21360 Mitochondrial DNA reveals distinct [more...]
Date: 2010-07-14 18:00:00
Genotyping of Chlamydia trachomatis strains from culture and ...
Mol Cell Probes . 2010 Oct 7; Ruettger A, Feige J, Slickers P, Schubert E, Morré SA, Pannekoek Y, Herrmann B, de Vries HJ, Ehricht R, Sachse K Current typing methods of Chlamydia (C.) trachomatis are mainly based on the diversity of the ompA gene, which is coding for the major outer membrane protein A. The present study aimed at facilitating genotyping of strains of this obligate intracellular human pathogen by developing a DNA microarray assay using the ArrayTube™ format for individual samples and the ArrayStrip™ format for higher throughput. See the original post here: Genotyping of [more...]
Date: 2010-10-12 21:11:53
Loyola University Chicago Stritch School of Medicine opens for ...
Loyola University Chicago is comprised of four campuses: Lake Shore (LSC), Water Tower (WTC), Medical Center, and the John Felice Rome Center in Italy; and is home to ten schools and colleges: arts and sciences, business administration, communication, education, graduate studies, law, medicine, nursing, continuing and professional studies, and social work. Loyola also serves as the U.S. host university to the Beijing Center for Chinese Studies in Beijing, China. Recognizing Loyola’s excellence in education, U.S.News and World Report has ranked Loyola consistently among the "top national [more...]
Date: 2010-10-11 13:33:50
Free Online Guides: Invasive honeysuckle increase risk of tick ...
It has sensory organs on its front two legs, so it'll stand there and wave those legs around trying to detect your heat and your carbon dioxide signature. And when it gets closer, it kind of zig zags as it's approaching you, because it's homing in on your signal and when it gets really close, it grabs on."Sometimes I'll just stand there and watch the ticks do this," he says grinning. "It's pretty amusing."My record trap, the one that blew the rest out of the water, had 5,000 nymph lifestage lone-star ticks on it. We've done capture studies that suggest the nymphs don't travel much more than [more...]
Date: 2010-10-13 15:20:00
Antibodies to DNA — NEJM
In the Farr assay, radiolabeled DNA is incubated with serum, and the DNA–anti-DNA complexes are precipitated with ammonium sulfate or polyethylene glycol. This assay may be the most specific test for systemic lupus erythematosus, ...... L. J. Mason, C. T. Ravirajan, D. S. Latchman, D. A. Isenberg. (2001) A human anti-dsDNA monoclonal antibody caused hyaline thrombi formation in kidneys of 'leaky' SCID mice. Clinical and Experimental Immunology 126:1, 137-142 [more...]
Date: 1998-05-07 07:00:00
Diagnosed With Genital Warts: Could This Have Been Misdiagnosed ...
The hc2 High-Risk HPV DNA Test™ (DNAwithPap™)* using Hybrid Capture2 (hc2) technology is an In Vitro nucleic acid hybridization assay with signal amplification using microplate chemiluminescence for the qualitative detection of thirteen high-risk types of human papillomavirus (HPV) DNA in cervical specimens. The HPV types detected by the assay are the high-risk HPV types 16/18/31/33/35/39/45/51/52/56/58/59/68… The hc2 High-Risk HPV DNA Test cannot determine the specific HPV type present. http://www.digene.com Genital warts can cause abnormal cell changes but most of these [more...]
Date: 2009-11-28 05:29:18
Research Scientist Sep 14, 2010 Posting date: April 2010 Description: Seeking energetic and meticulous Research Scientist to lead a team in developing DNA purification protocols and real-time PCR assays to support Spartan’s real-time DNA analyzer. Responsibilities will include collaborating with industry and research partners, managing technicians, and writing technical notes and publications. Microbiology, virology and experience in food safety an asset. Qualifications: Ph.D. in Biological Sciences Peer-reviewed publications Extensive experience with molecular biology techniques, [more...]
Date: 2010-09-14 22:04:55
PLANT TISSUE CULTURE TECHNIQUE AND PCR TECHNOLOGY
SSR assays require a minimal amount of genomic DNA. Most of the crop improvement programs are confined to evaluation and selection of naturally occurring clonal variations. In vitro culture techniques provide an alternative means of plant propagation and an important tool for crop improvement. The in vitro cultures are also of low risk for genetic variation since it is more resistant to genetic changes while occurrence of cell division in vitro condition. Gel electrophoresis is an important molecular biology tool which enables us to study DNA. It can be used to determine the sequence of [more...]
Date: 2010-09-08 04:07:38
An ELISA-based high throughput protein truncation test for ...
Here, we report the next generation HTS-PTT using triple-epitope-tagged proteins and demonstrate, for the first time, its efficacy on clinical genomic DNA samples for BRCA1/2 analysis. Methods: Segments of exons 11 of BRCA1/2 open reading frames were PCR amplified from either blood derived genomic DNA or cell line mRNA. PCR primers incorporate elements for cell-free transcription/translation and epitope tagging. Cell-free expressed nascent proteins are then antibody-captured onto the wells of a microtiter plate and the relative amount of truncated polypeptide measured using antibodies against [more...]
Date: 2010-10-04 16:47:13
Research Technician Sep 14, 2010 Posting date: April 2010 Description: Seeking energetic and meticulous Research Technician to develop DNA purification protocols and real-time PCR assays for Spartan’s DNA analyzers. Responsibilities will include designing, performing, and analyzing experiments. Qualifications: B.Sc. in Biological Sciences Experience with molecular biology techniques, preferably including real-time PCR Strong interpersonal and communication skills To apply, please forward your resume to [more...]
Date: 2010-09-14 22:08:03
Thrombosis Journal | Full text | Expression of sterol regulatory ...
DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]
Date: 2009-02-18 08:00:00
Coralville's IDT Partners with Marshall University
The Marshall Institute for Interdisciplinary Research (MIIR) has announced it has entered into an applied research and product development partnership with leading biotechnology company Integrated DNA Technologies (IDT). According to the terms of the agreement, scientists at MIIR will be developing optimized biomolecular analyses or “assays” to be used by IDT for the detection and quantification of ribonucleic acid (RNA). The goal of the co-development project is to significantly improve the specificity of IDT’s current assay methods without substantially increasing the cost. IDT’s [more...]
Date: 2010-05-21 20:59:12
High-Content Fluorescent-Based Assay for Screening Activators of ...
Activation of DNA damage checkpoint pathways, including Chk2, serves as an anticancer barrier in precancerous lesions. In an effort to identify small-molecule activators of Chk2, the authors developed a quantitative cell-based assay using a high-content analysis (HCA) platform. Induction of phosphorylated Chk2 was evaluated using several different parameters, including fold induction, Kolmogorov-Smirnov score, and percentage of positively stained cells. These measurements were highly correlated and provided an accurate method for compound ranking/binning, structure-activity relationship [more...]
Date: 2008-06-19 07:00:00
Comet Assay from AMSBIO
Out of this world DNA damage assays from AMSBIO. See DNA fragmentation in a whole new way. To learn more about Comet Assay, go to : www.amsbio.com Video Rating: 4 / [more...]
Date: 2010-09-18 13:54:45
The effect of DNA signals on nucleosome formation, chromatin ...
Much of the human genome is non-coding and it seems likely that at least some of the non-coding DNA is functionally important. However, only little is known about the possible function of the bulk of the human genome. Since essentially all the nuclear DNA is packaged into chromatin, it is likely that functional effects of non-coding DNA are mediated by the chromatin structure. By making use of human genomic sequence information now available, we showed that a specific set of periodic DNA motifs (period-10 VWG/CWB) encoded in genomic DNA is predicted to influence human chromosome function. The [more...]
Date: 2010-08-25 23:15:17
JAK2 V617F Mutation in Unexplained Loss of First Pregnancy — NEJM
Meta-analyses and reviews1 suggest that disorders promoting venous thrombosis, collectively termed “thrombophilias,” contribute to the pathogenesis of fetal loss. The high variability among studies with respect to the associated risk between such ... Women were screened by means of a nested allele-specific polymerase-chain-reaction assay performed on DNA extracted from circulating leukocytes. Multiparametric conditional logistic-regression analysis was performed. [more...]
Date: 2007-11-07 21:55:30
Improving Molecular Detection of Fungal DNA in Formalin-Fixed ...
assays, followed by sequencing of the DNA fragments ob- tained. The protocols were assessed for time spent in perform- ing the procedure, quality of DNA detection, and efficiency of fungal-DNA detection. MATERIALS AND METHODS [more...]
Date: 2010-08-27 20:37:26
To obtain these structures, a new, efficient solid-phase synthesis protocol was developed as well. To determine the binding affinity and selectivity of these compounds among all possible 4-base pair sequences of DNA, a high throughput (HT), fluorescence intercalator displacement (FID) assay was employed. The FID results indicate that these compounds are good DNA minor groove binders as predicted (more than 50% fluorescence decrease at 10 μM for A/T only sequences); the amidine group improves the binding affinity and selectivity compared to amino acid-benzimidazole conjugates without an [more...]
Date: 2010-08-25 22:40:17
Standardized Comet Assay System For Detection of DNA Damage ...
The AMSBIO range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signalling, DNA damage, electrophoresis, glycobiology, post translational modification and stem cell biology. Worldwide HQ AMS Biotechnology (AMSBIO) 184 Milton Park Abingdon Oxon OX14 4SE UK Tel: +44 1235-828200 Fax: +44 1235-820482 Email: email@example.com Web: [more...]
Date: 2010-09-18 07:00:00
Sperm DNA damage measured by the alkaline Comet assay as an ...
Date: 2010-09-22 07:00:00
Prediction of the Risk of Myocardial Infarction from Polymorphisms ...
We used a fluorescence- or colorimetry-based allele-specific DNA-primer–probe assay system to determine the genotypes of 112 polymorphisms of 71 candidate genes in 2819 unrelated Japanese patients with myocardial infarction (2003 men and 816 women) ...... (2004) An association analysis between genetic polymorphisms of matrix metalloproteinase-3 and methylenetetrahydrofolate reductase and myocardial infarction in Japanese. Journal of Thrombosis and Haemostasis 2:3, 527-528 [more...]
Date: 2002-12-12 05:00:00
Whole blood and tissue fungal DNA quantification in the diagnosis ...
3.2. Fungal DNA quantification. Fungal DNA was detected by the PenAsp assay in all nasal biopsy samples except in the samples from two dogs with nasal neoplasia (Fig. 1). Although the relative copy numbers overlapped between the four [more...]
Date: 2008-10-01 07:00:00
A non-radioactive, PFGE-based assay for low levels of DNA double ...
A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells This digital document is a journal article from DNA Repair, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: A PFGE method was adapted to measure DNA double-strand breaks (DSBs) in mammalian [more...]
Date: 2010-09-10 23:55:39
Rapid Detection of Respiratory Tract Viral infections and Co ...
Respiratory samples were tested using the combination of two commercially available RT-PCR DNA microarray systems allowing a rapid detection of influenza A strains including the new A/H1N1v strain as well as twenty other common or newly discovered respiratory viruses. Concomitantly a generic and classical real time RT-PCR assay was performed to detect all the circulating influenza A strains in the same samples. Of the 95 respiratory samples tested, 30 (31%) were positive for the detection of influenza A/H1N1v infection by both RT-PCR DNA microarray and classical real time RT-PCR detection [more...]
Date: 2010-08-28 06:34:00
Whole-Genome Sequencing in a Patient with Charcot–Marie–Tooth ...
DNA-sequencing methods that involve chemical assays other than the traditional Sanger dideoxy-chain-termination method. Next-generation-sequencing methods produce much larger quantities of data at less expense, but the individual raw [more...]
Date: 2010-03-31 20:55:45
gc chlamydia dna probe
Chlamydia / GC, DNA Probe w / Rflx? is a bill that I have in the word "description" It's a long series of tests that the tests are completed routine blood tests. DNA probe assays for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) or commonly called (STD) sexually [more...]
Date: 2010-06-18 08:07:48
Neurokinin-1 Receptor Antagonist Treatment in Polymicrobial Sepsis ...
Sample concentration was estimated from the standard curve. DNA assay was performed fluorometrically by using Hoechst dye 33256 . The sample concentration was then corrected for the DNA content of the tissue . [more...]
Date: 2010-08-07 07:00:00
Development of a Novel Fluorescence Assay Based on the Use of the ...
Human -alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the position of guanine. ... Oligonucleotide synthesis was performed by the solid-phase - cyanoethyl-phosphoramidite method . Ammonia treatment was performed at room temperature overnight to minimize decomposition of O6-methylguanine. For the same reason, the dimethylformamidino group was selected for the protection of [more...]
Date: 2010-07-17 07:00:00
DNA Preparation of Bone Marrow Sample for Backtracking | Cancer ...
To isolate DNA from supernatant, the 500µl of 2X concentration of PBS is used.4. 500µl of PCI was added into the tube for 30 minutes.5. Later, the tube was spun at 10000X ϑ for 10 minutes.6. The aqueous supernatant was removed to a new tube without disturbing the PCI layer.7. The PCI layer was discarded and add in 1:10 volume of 10M NH4OAc into the tube to mix.8. Next, the 2X volume of ethanol was added and mixed up the ethanol and precipitated DNA.9. The ethanol was removed from the mixture. The precipitation was washed one time with 500µl 70% ethanol.10. Again, the ethanol was removed [more...]
Date: 2010-07-16 08:22:19
Biotechnology, XII: Model Paper « Home works and Assignments Online
Which vectors are used in human genome sequencing & why? Explain the blue white method of screening the recombinant plasmid? A single cell of E.coli is supposed to produce about 2000 different proteins. under optimum conditions. One of the desired intercellular enzymes produced is in the form of 2500 molecules/cells. If the molecular weight of that enzyme is 100000D.How many E.coli cells are required to produce 1gm of enzymes. What are secondary metabolites? Name the secondary metabolites produced by the following plants. (a)Atropa beladona (b) Cinchona officinalis. SECTION C (3 marks [more...]
Date: 2010-10-13 05:07:00
Predictive Molecular Pathology — NEJM
However, fresh tissue is highly preferable for DNA-microarray analysis, and its use may become mandatory in future assays to allow investigation of the status of drug targets. A global change in the handling of tumor tissue is [more...]
Date: 2009-03-27 14:12:49
Expression, Purification and Activity Assay of the Recombinant ...
about 700 bp was purified by DNA purification kit. Both the purified cDNA fragment of the Fc-COMT gene and pET-30a(+) vector were cut individually by. EcoRI and XhoI The digested products were separately purified using gel purification [more...]
Date: 2010-09-03 08:10:44
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are involved in various disease problems in humans and aquatic animals and are known to be phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific diagnostic test is warranted for detection and characterization of this pathogen.In the present study, Fish and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]
Date: 2010-10-08 20:08:05
Natural Health News: Supplement Suppresses Breast Cancer Cells
Expression of cell cycle regulatory genes were determined by DNA-microarray analysis. Effect of BD on invasiveness was assessed by cellular adhesion, migration, and invasion assays. Results. BD treatment of cells MDA-MB-231 resulted in the cytostatic inhibition of cell proliferation with IC50 22.2, 19.1, and 17.5 μg/mL for 24, 48, and 72 hours, respectively. The inhibition of proliferation was mediated by the upregulation expression of CCNG1, CHEK1, CDKN1C, GADD45A, and E2F2, whereas BD downregulated expression of CCNA1 and CDK6 genes. The induction of expression of GADD45A and inhibition of [more...]
Date: 2010-10-11 16:51:00
IQGAP1 translocates to the nucleus in early S-phase and ...
In support of this, we used immunoprecipitation assays to show that the nuclear pool of IQGAP1 in G1/S-arrested cells associates with DNA replication complex factors RPA32 and PCNA. More important, the siRNA-mediated silencing of IQGAP1 [more...]
Date: 2010-10-06 13:10:04
The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...
There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]
Date: 2010-10-04 21:15:46
Alnylam Scientists Present New Data on RNAi Therapeutics at ...
Further, several of the presentations describe the design, synthesis, and analytical characterization of siRNAs with optimized properties, including: “Solid-phase synthesis of 5'-di- and tri-phosphates and their modified analogs of DNA, RNA and chemically modified oligonucleotides,” by Ivan Zlatev, Ph.D., Scientist; “Parallel high throughput synthesis of chemically modified 21-27mer siRNA sequences,” by Satya Kuchimanchi, Ph.D., Associate Director, Small Scale Synthesis; “Novel method for the confirmation of siRNA sequence by LC-MS/MS,” by Gary Lavine, Ph.D., Senior [more...]
Date: 2010-08-24 12:13:50
Variants of PCR (1)
The bacteriophage T4 DNA polymerase was also tested shortly after the first reports of PCR. It has a higher fidelity of replication than the Klenow fragment. Since it is also destroyed by heat, it has seen little use since the development of thermostable polymerases. The DNA polymerase from Thermus aquaticus (or Taq), was the first thermostable polymerase used in PCR, and is still the one most commonly used. The enzyme can be isolated from its 'native' bacterial source, or from a cloned gene expressed in E. coli. The Stoffel fragment is produced from a truncated gene for Taq polymerase, [more...]
Date: 2008-09-11 03:56:00
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading
Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]
Date: 2009-11-13 08:00:00
Cytogenetics Clinical Lab Technologist Job / Mayo Clinic ...
Knowledge of genetics, molecular genetics, DNA extraction, PCR, microscopy, cell culture and aseptic technique is desirable. Experience with operation and troubleshooting of laboratory instrumentation and assay systems is desirable. CV/RESUME, COVER LETTER, and TRANSCRIPTS, unofficial copy accepted, MUST ALL BE INCLUDED FOR YOUR APPLICATION TO BE CONSIDERED. **A 2 YEAR COMMITMENT TO THIS POSITION IS REQUIRED. Benefit Eligibility: Yes Posting Begin Date: 08/06/2010 Posting End Date: Until Filled Employment Type: Non Exempt Hours/2-Weeks: 80 Schedule: Monday - Thursday Shift: [more...]
Date: 2010-10-09 21:08:40
IVF News - The Sperm Chromatin Structure Assay (SCSA?) Test ...
The Sperm Chromatin Structure Assay (SCSA®) test measures the percent of sperm in a semen sample that has fragmented DNA as well as the extent of DNA fragmentation. In the SCSA® test, sperm with very low levels of fragmentation fluoresce green, while sperm with moderate to high levels of fragmentation fluoresce red. The flow cytometer measures the ratio of red to green fluorescence in each of 5000 sperm. The percent of sperm with DNA fragmentation (red fluorescence) is expressed as the DNA Fragmentation Index (DFI; Evenson et al 2002). The SCSA® test is a rapid, consistent, statistically [more...]
Date: 2003-01-08 08:00:00
DNA method gives new perspective on the Mysteries of Nature
We demonstrate that this new approach enables the assignment of virtually all the generated DNA sequences to the correct source once sequencing anomalies are accounted for (miss-assignment rate less than 0.4%). Therefore, the method enables accurate sequencing and assignment of homologous DNA sequences from multiple sources in single high-throughput GS20 run. We observe a bias in the distribution of the differently tagged primers that is dependent on the 5' nucleotide of the tag. In particular, primers 5' labelled with a cytosine are heavily overrepresented among the final sequences, while [more...]
Date: 2007-02-15 17:01:00
Blog: Behavior Ecology And Sociobiology » Plant Molecular Biology ...
Plant Cell Reports 2001 , 20:731-737. Publisher Full Text Olhoft P, Somers D: L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Reports 2001 , 20:706-711. Publisher Full Text Trick HN, Finer JJ: Sonication-assisted Agrobacterium-mediated transformation of soybean embryogenic suspension culture tissue. Plant Cell Reports 1998 , 17:482-488. Publisher Full Text de Oliveira MLP, Febres VJ, Costa MGC, Moore GA, Otoni WC: High-efficiency Agrobacterium-mediated transformation of citrus via sonication and [more...]
Date: 2003-08-01 07:00:00
HTB – Early infant diagnosis
She concluded that promising POC assays for EID today include: IQuum’s LIAT, SAMBA, CIGHT p24 and possibly CLONDIG’s viral load assay. comment This was an incredibly useful overview and it looks like we can be optimistic about having a POC test for EID in the next couple of years. Other presentations at the conference dealt with the challenges of access to EID. In the same session, Shaffiq Essajee noted that EID access has improved and in some countries more than 50% of exposed infants are tested. But infant testing is usually linked to PMTCT and if coverage is low, so is infant [more...]
Date: 2010-09-30 06:04:59
Ph.D or post-doc assistantship in plant evolution/biogeography at ...
The successful applicant will choose one or more of these plant families to: generate flow cytometry data aimed at determining ploidy levels; generate nuclear DNA sequences and infer species trees; incorporate ecological data to investigate issues of niche conservatism vs. niche evolution in the selected groups. Requirements: Demonstrated experience in molecular, phylogenetic and flow-cytometry methods and/or ecological niche modeling will be highly valued in the selection process. Excellent knowledge of the English language, written and oral, required. Familiarity with additional European [more...]
Date: 2010-06-04 13:05:00