Top 10 Cutting-Edge Advances in CSI Technology
Investigators itching to retrieve a fingerprint, but hesitant to disturb material that could be subjected to DNA analysis, may soon be able to use a non-contact retrieval technique. 5. Portable Laser How many people can brag that they carry a portable laser to work? Now smaller, lighter, and more powerful than ever, investigators can use this high-tech tool to find more trace evidence and process crime scenes faster, and we all know that time is of the essence in crime fighting. Nothing short of amazing, even a miniscule piece of a nearly-invisible blond hair found using laser [more...]
Date: 2010-10-06 16:59:00
DNA Art Blog » Blog Archive » PCR Process
We use primer sets that are optimally designed to span the specific genes you choose to have isolated for your canvas. Our technique focuses on the prevention of pre-PCR mis-priming and primer dimerization. Our primers are designed using software with algorithms that check for the potential of secondary structure formation and annealing of primers to itself or within primer pairs. We use hot start Taq Polymerase is designed to enhance the specificity, sensitivity, and yield of the target DNA sequence. Following PCR amplification and purification of the template DNA, we use restriction [more...]
Date: 2010-10-12 15:19:03
HTB – Early infant diagnosis
She concluded that promising POC assays for EID today include: IQuum’s LIAT, SAMBA, CIGHT p24 and possibly CLONDIG’s viral load assay. comment This was an incredibly useful overview and it looks like we can be optimistic about having a POC test for EID in the next couple of years. Other presentations at the conference dealt with the challenges of access to EID. In the same session, Shaffiq Essajee noted that EID access has improved and in some countries more than 50% of exposed infants are tested. But infant testing is usually linked to PMTCT and if coverage is low, so is infant [more...]
Date: 2010-09-30 06:04:59
Variants of PCR (1)
The bacteriophage T4 DNA polymerase was also tested shortly after the first reports of PCR. It has a higher fidelity of replication than the Klenow fragment. Since it is also destroyed by heat, it has seen little use since the development of thermostable polymerases. The DNA polymerase from Thermus aquaticus (or Taq), was the first thermostable polymerase used in PCR, and is still the one most commonly used. The enzyme can be isolated from its 'native' bacterial source, or from a cloned gene expressed in E. coli. The Stoffel fragment is produced from a truncated gene for Taq polymerase, [more...]
Date: 2008-09-11 03:56:00
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING ...
GENOMIC CHARACTRIZATION OF AEROMONAS HYDROPHILA ISOLATES USING RAPD-PCR TECHNIQUE by Mitali Dhiman and S. S. Mishra Central Inland Fisheries Resear Aeromonas hydrophila are involved in various disease problems in humans and aquatic animals and are known to be phenotypically, serologically and genetically quite diverse. Development and use of a sensitive and specific diagnostic test is warranted for detection and characterization of this pathogen.In the present study, Fish and water samples from river Hooghly were streaked onto Aeromonas selective growth medium (Rimler-shotts [more...]
Date: 2010-10-08 20:08:05
EpiCentral: Examining blocking lesions in ancient DNA
Blocking lesions prevent amplification and sequencing of affected molecules, thus limiting the analysis of DNA derived from ancient samples. Heyn et al. recently developed a new method--polymerase extension profiling (PEP)--that reveals occurrences of polymerase stalling on DNA templates. This sequencing-based technology allows detection of damage on a single-molecule level. The technique used CircLigase™ ssDNA Ligase for high-efficiency ligation of single-stranded adaptors (containing the Roche 454 A sequence) to the 3’ ends of primer-extension products. The authors found evidence of [more...]
Date: 2010-10-07 20:29:00
Bio Saga Blog: 5-Day Hands-on Workshop on Molecular Biotechnology ...
Recombinant DNA Technology: Cutting & pasting of DNA molecules (i.e. restriction digestion, ligation, DNA gel eectrophoresis, and gel extraction.) Bioinfo part: Bioinformatics of DNA database / SequenceAnalysis: Pairwise sequence alignment, Multiple sequence alignment, Pattern search.Tools: BLAST, CLUSTALW/X, CLC Bio Main Workbench Genetic Engineering: Transformation and plasmid purification, cloning and sub-cloning, amplification of DNA fragments by polymerase chain reaction (PCR). Bioinfo part: Designing of PCR primers In-silico cloning Searching for homologous and paralogous [more...]
Date: 2010-09-29 05:41:00
Assessment of nematode biodiversity using DGGE of 18S rDNA ...
Recently, extraction of soil DNA, amplification of 18S rDNA genes using nematode consensus primers and subsequent separation by denaturing gradient gel electrophoresis (DGGE) has been used to estimate nematode diversity in soil. Here, we investigate an alternative approach whereby nematodes are first extracted from the soil prior the 18S rDNA gene amplification using universal primers. We used this system to estimate nematode species richness in 10 soil samples-five from Scotland and five from the Netherlands. There was no direct correlation between species richness as estimated [more...]
Date: 2010-09-22 06:07:53
Specific replication origins promote DNA amplification in fission ...
We propose that these features predispose replication origins to re-fire within a single S phase, or to remain active after passive replication. Key words: Cell cycle, DNA amplification, DNA replication, Fission yeast, [more...]
Date: 2010-09-01 16:30:57
ISPUB - Comparison and Development of A Rapid Extraction Methods ...
Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological, Archaeological, Museum, Medical and Forensic Specimens. New York, NY: Springer Verlag. (s)7. Holland, M.M. and T.J. Parsons. 1999. Mitochondrial DNA sequence analysis— validation and use for forensic casework. Forensic Science Review. 11: 22 – 49. (s)8. Matysiak-Scholze,U., Dimmeler,S. and Nehls,M. (1996) Technical Tips Online, T40011. (s)9. Near T.J., Bolnic D.I. and Wainwright P.C., Fossil calibrations and molecular divergence time estimates in centrarchid fishes, Evolution, 2005, 59(8): 1768-1782 (s)10. Paabo [more...]
Date: 2010-06-06 01:06:13
Genes | Free Full-Text | The Application of Next Generation ...
Recently various high-throughput approaches based on bisulfite conversion combined with next generation sequencing have been developed and applied for the genome wide analysis of DNA methylation. These methods provide single base pair resolution, quantitative DNA methylation data with genome wide coverage. We review these methods here and discuss some technical points of special interest like the sequence depth necessary to reach conclusions, the identification of clonal DNA amplification after bisulfite conversion and the detection of non-CpG methylation. Future application of these methods [more...]
Date: 2010-06-04 07:00:00
A day in the life of… Sara
I prepare the appropriate master mixes and primer probes for my samples to prepare them for DNA amplification. I can test for DNA amplification, and therefore if my primers worked, by running an agarose gel electrophoresis on the PCR product against DNA ladder standard material. After sending an electrical current through my gel and buffer, I observe how chunks of DNA from the PCR product have moved (or raced) through the gel relative to the standards (I hope to see a tie at a certain ‘ladder step’ in each lane). At this point in my day I secretly do a celebratory fist pump and heel [more...]
Date: 2010-07-22 23:14:43
Origin of the human malaria parasite in gorillas - TalkRational
But researchers from the US, three African countries, and Europe have examined malaria parasites in great ape faeces. They found the DNA from western gorilla parasites was the most similar to human parasites. Liu, W. et al. (2010) Origin of the human malaria parasite Plasmodium falciparum in gorillas. Nature, 467, 420-425. Plasmodium falciparum is the most prevalent and lethal of the malaria parasites infecting humans, yet the origin and evolutionary history of this important pathogen remain controversial. Here we develop a single-genome amplification strategy to identify and characterize [more...]
Date: 2010-09-22 17:35:43
Genome Biology | Full text | Optimization and clinical validation ...
Novel features of this platform include the development of a robust algorithm that accurately predicts PCR bias during DNA amplification and can be used to improve PCR primer design, as well as a powerful statistical concept for inferring .... This probability is reduced when intra-primer hairpin formation is predicted, and increased according to degree of complementarity between tag sequence and viral sequence. In this manner, the probability that each nucleotide will be [more...]
Date: 2007-05-27 00:00:00
SNAGG Technology: New Micro Chip Offers Huge Anti-Theft Benefits ...
David Nordschow Amplification, LLC to use SNAGG technology in all products We’ve all heard the personal horror stories about players losing a favorite instrument, or having their entire stage rig stolen and their lives totally disrupted. For owners of one company’s products, the danger of gear loss will be lowered significantly. David Nordschow Amplification, LLC (DNA) announced that it will incorporate anti-theft technology into every product it builds. Partnering with Snagg Technology, DNA is the first bass amplification company to offer RFID (Radio Frequency Identification) technology [more...]
Date: 2010-10-11 08:01:30
DNA sequencing in drug discovery: Market prospects for ...
Covers microarrays, DNA sequencing kits, chromatography, mass spectrometry, DNA amplification, and many other instruments in the market, along with its sub-segments. The market includes high-performance sequencing, [more...]
Date: 2010-07-22 02:22:58
Ensuring Successful PCR Using Online Resources-eNotes
This system quantitates DNA prior to multiplex short tandem repeat (STR) amplification, a method used for DNA typing, including paternity testing, forensic DNA analysis and human identity testing. [more...]
Date: 2009-04-14 00:00:01
Hisar Practical Training Course Recombinant DNA Techniques ...
Date: 2010-10-05 04:07:03
In Vivo Assembly of DNA Constructs-Columbia Technology Ventures
Eliminates the use of restriction enzymes and many PCR amplification steps by employing the use of double stranded DNA breaks to trigger in vivo homologous recombination • Equally applicable to small as well as large DNA fragment assembly • The cycling of only two selectable markers and the use orthogonal endonucleases allows for repeated rounds of assembly of many DNA fragments • The assembled DNA is in a known location of the yeast genome and can thus be easily transferred from one strain to another Patent Status: PCT/US10/32962 Licensing Status: Available for Licensing and Sponsored [more...]
Date: 2010-10-02 16:40:33
Graduate/Postdoctoral researcher in Ravenna- University of Bologna ...
DNA extraction, - PCR amplification of mitochondrial / nuclear loci – sequencing – database management, - troubleshooting during laboratory analyses. Candidates should have a Master's Degree in biological science or related fields. [more...]
Date: 2010-08-11 09:00:43
Epigenetics Papers: DNA Methylation Analysis by MethyLight Technology
Authors discussed each step for this procedure: (1) determining the site of interest for methylation analysis; (2) methylation-specific primers and fluorogenic probes design; (3) genomic DNA isolation; (4) bisulfite conversion; (5) real- time methylation analysis; and (6) data processing. For primer design, some complications particular to the bisulfite modification should be taken into account: All unmethylated cytosine residues in the genome are converted this reduction in genomic complexity reduces the annealing specificity of PCR primers and fluorogenic probes, which not only [more...]
Date: 2007-08-19 01:26:00
Software Packages presenti in Ubuntu 10.04 Lucid Lynx Categoria ...
ncbi-epcr (2.3.12-1) : Tool to test a DNA sequence for the presence of sequence tagged sites; ncbi-rrna-data (6.1.20090809-1) : large rRNA BLAST databases distributed with the NCBI toolkit .... poa (2.0+20060928-2) : Partial Order Alignment for multiple sequence alignment; praat (5.1.25-1) : program for speech analysis and synthesis; primer3 (1.1.4-1) : Tool to design flanking oligo nucleotides for DNA amplification [more...]
Date: 2010-09-12 09:46:00
Evaluation of Five Methods for Total DNA Extraction from Western ...
DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. Methodology/Principal Findings From individual western corn rootworm beetles, Diabrotica virgifera virgifera, DNA extractions by the SDS method, CTAB method, DNAzol® reagent, Puregene® solutions and DNeasy® column were compared in terms of DNA quantity and quality, cost of materials, [more...]
Date: 2010-08-13 07:00:00
IVF Mail - single cell DNA Extraction
Hi all.. please can you tell me how we can extract DNA from single cell for downstream amplification using real time PCR (PGD analysis). please if you know tell me the name of such kit used and the company manufacture it. really I am very interested in this [more...]
Date: 2010-10-04 22:33:19
Chemical synthesis of the mouse mitochondrial genome « too New to yoU
However, this study used the basic synthesis unit is a nucleotide containing thousands of DNA (deoxyribonucleic acid) fragments. Because the basic synthesis unit larger, the final synthesis in the verification is correct the genome will bring some trouble. Thus, the research team invented a new method of synthesis of the genome, using the basic synthesis unit is only 60 nucleotides of DNA containing fragments, placing them well-designed environment, you can connect into the whole genome. The group believes that this is by far the easiest way to synthetic genome. In order to verify the [more...]
Date: 2010-10-13 07:19:52
(IUCr) Expression, purification and crystallization of Chaetomium ...
The copy number of the gene integration in the genome of Pichia pastoris was determined to be between 7 and 12. PCR amplifications were carried out based on genomic DNA using primers corresponding to [more...]
Date: 2010-08-28 07:00:00
DNA Amplification System
Buyer: the Republic of Korea (PPS) Public Procurement [more...]
Date: 2010-06-07 05:43:50
PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading
Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]
Date: 2009-11-13 08:00:00
Diagnosed With Genital Warts: Could This Have Been Misdiagnosed ...
The hc2 High-Risk HPV DNA Test™ (DNAwithPap™)* using Hybrid Capture2 (hc2) technology is an In Vitro nucleic acid hybridization assay with signal amplification using microplate chemiluminescence for the qualitative detection of thirteen high-risk types of human papillomavirus (HPV) DNA in cervical specimens. The HPV types detected by the assay are the high-risk HPV types 16/18/31/33/35/39/45/51/52/56/58/59/68… The hc2 High-Risk HPV DNA Test cannot determine the specific HPV type present. http://www.digene.com Genital warts can cause abnormal cell changes but most of these [more...]
Date: 2009-11-28 05:29:18
Oligonucleotide Synthesis: Methods and Applications (Methods in ...
Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene [more...]
Date: 2004-07-30 07:00:00
Human Epidermal Growth Factor Receptor 2 Testing: Where Are We? — JCO
Fig 1. Schematic representation of evaluation of HER2/neu amplification/overexpression and rationally designed, targeted therapeutic options. qRT-PCR, quantitative reverse transcription polymerase chain reaction; FISH, fluorescent in situ hybridization; IHC, immunohistochemistry; PI3K, phosphatidylinositol 3-kinase; AKT, protein kinase B; mTOR, mammalian target of rapamycin. Amplification is the primary mechanism of HER2 overexpression.5 Gene amplification was initially detected by Southern hybridization in frozen tumor specimens and was subsequently found to correlate with overexpression at [more...]
Date: 2010-09-29 22:01:53
ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...
Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]
Date: 2010-08-25 00:51:13
Research Associate (San Diego) | PCR Jobs
"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:Â Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.Â Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.Â Prepares technical reports, summaries, protocols and quantitative analyses.Â Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]
Date: 2010-10-01 19:07:24
Omics! Omics!: What should freshman biology cover?
I can think of some problem set items that can be jettisoned (Maxam-Gilbert sequencing is a historical curiosity at this point; I can think of much more relevant procedures to do on paper). One topic I've convinced myself belongs in the early treatment is the proteasome, and not because I once spent a lot of time thinking about it (and also saw some financial gain -- though I no longer have such an interest in it). This is definitely a field which didn't exist on solid ground when I went through school, so it's absence from my early education First, it fits neatly into one of the key themes [more...]
Date: 2010-03-23 23:23:00
DNA fingerprinting simplified
One of the most frequently used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic technique often used in genetic or DNA fingerprinting. The procedure is achieved by moving negatively charged nucleic acid molecules through a gelatinous substance known as agarose by using an electric field. Andrew, in collaboration with his father and other researchers, completed the study, which is published in the January 2008 issue the American Society of Horticultural Science’s journal HortTechnology. The youngest author ever to publish in an ASHS journal, Andrew [more...]
Date: 2008-05-14 04:52:12
Thrombosis Journal | Full text | Effect of PlA1/A2 glycoprotein ...
Genomic DNA was prepared from peripheral lymphocytes by the salt precipitation method . The PlA1/A2 alleles of the GP IIIa gene were identified on the basis of MspI enzyme site restriction analysis after amplification of a 476 base pairs GP IIIa fragment (sens amorce 5'-ATA-AGC-TTA-GCT-ATT-GGG-AAG-TGG-TAG-GGC-CTG-3', antisens amorce 5'-CTT-CTG-ACT-CAA-GTC-CTA-ACG-3'). The GP IIIa gene was amplified using the polymerase chain reaction (PCR) method. Each amplification product was verified on an agarose gel. Amplification results in a 476 base pairs (bp) fragment. Digestion was obtained with [more...]
Date: 2008-01-15 08:00:00
Heliscope builds world's fastest DNA sequencer | Singularity Hub
The Short: Technology Review reports that for just $1,350,000 USD you can have have the worlds fastest commercially available DNA sequencer, called the Heliscope. The machine developed by Helicos BioSciences takes just one hour to read 1.3 billion base pairs from a strand of DNA. Here is a picture of the beauty: HeliScope™ Single Molecule Sequencer The Long: The Heliscope is being marketed as a DNA microscope. It is unique in the field of DNA sequencing because it sequences an actual DNA strand whereas most other sequencing technologies use PCR to create millions of copies of an original [more...]
Date: 2008-07-14 16:58:33
Colony PCR: Another Use For Toothpicks « Promega Connections
Colony PCR: Another Use For Toothpicks July 26, 2010 by Isobel I still remember my JOY (sadly, yes) when I first learned about colony PCR. It allowed me to avoid a laborious procedure involving genomic DNA isolation, restriction digestion, and (the dreaded) Southern blotting. I was trying to show definitively that a transposon had successfully been inserted into a target gene. Using colony PCR, I could just amplify the DNA from a colony and then show that the transposon increased the size of the expected PCR product compared to a control. Joy. I could also use colony PCR to screen DNA [more...]
Date: 2010-07-26 15:13:31
Ultrasensitive DNA detection
Rapid and sensitive DNA detection methods have a wide range of uses from cancer and disease diagnosis to the detection of harmful bacteria in food and drink. Previous methods rely on optical methods and a time consuming pre-amplification of the sample, which while sensitive often take several weeks and require bulky apparatus. Electrochemical methods have been used to improve the speed and portability. Now, Elena Ferapontova, Kurt Gothelf and colleagues at Aarhus University have developed a lipase-based electrochemical technique that can detect as little as 20 attomoles (2 x 10-17 moles) of [more...]
Date: 2010-03-12 23:37:21
Is free, circulating DNA a useful marker for prostate cancer ...
The "New" Prostate Cancer InfoLink has been developed to become a primary source of accurate, current, and topical information about prostate cancer for patients and their families. The "New" Prostate Cancer InfoLink is intended for informational purposes only. It is not engaged in rendering medical advice or professional services. News and information provided on this site should not be used for diagnosing or treating any health problem or disease. The "New" Prostate Cancer InfoLink is not a substitute for professional care. If you have or suspect you may have a health problem, please [more...]
Date: 2010-09-28 12:30:40
FDA Approves Roche's Hepatitis B Viral Load Test | InfoGrok
The new Roche test provides a fully automated solution for the quantitative detection of hepatitis B virus (HBV) DNA in human plasma or serum for patients on HBV antiviral therapy. The Roche COBAS AmpliPrep / COBAS TaqMan HBV Test v2.0 has been validated to quantify diverse samples from genotypes A-H and pre-core mutants across a broad linear dynamic range of 20 IU/mL to 1.7E+08 IU/mL. The new assay uses a reduced sample input volume of 650 uL of either serum or plasma specimens and is standardized against the World Health Organization (WHO) standard for hepatitis B. This test is designed for [more...]
Date: 2010-09-23 14:10:06
GE Awards Microbiome Grants for Whole Genome Amplification of ...
The second grant, good for $440,000 over two years, will enable Stanford University scientists to develop an approach for plating, selecting, and amplifying whole genomic DNA from individual microbial cells in a hydrogel matrix, according to the grant’s abstract. And although the two grants collectively represent a little under $1 million of the approximately $42 million worth of grants recently awarded by the NIH for human microbiome research, they may help address many of the inherent technical difficulties of isolating, amplifying, and sequencing genetic material from microorganisms that [more...]
Date: 2010-09-10 19:33:53
CIENCIASMEDICASNEWS: Recurrent Granulibacter bethesdensis ...
DNA was isolated from human tissue by using the Maxwell 16 Tissue DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. DNA concentrations were measured by using a UV spectrophotometer (NanoDrop, Wilmington, DE, USA). The 16S rRNA and methanol dehydrogenase subunit 1 (GeneID YP_744165.1) genes of G. bethesdensis were analyzed by using a PCR and primer sequences 16S-forward: 5'-TCGGGTGGGCACTCTAAAGG-3', 16S-reverse: 5'-GCATCACTGCCTAGCTTCCC-3', MDH-forward: 5'-CCGCAATACGGTCAATTCG-3', and MDH-reverse: 5'-GCCGATCTTCCAGGTTTCTTC-3'. Each reaction mixture [more...]
Date: 2010-09-02 23:42:00
Fastest Evolving Technology – DNA Sequencing | Impact Lab
Human DNA sequence Investing in technology-driven fields is risky, especially when everyone touts them as the Next Big Thing. Sure, it’s easy to see quick gains, but you’re just as likely to see those gains vanish as the next-generation technology sneaks in and replaces it — the disruptor becomes the disrupted, so to speak. Perhaps the fastest evolving technology right now isn’t computer tech, but rather is found in DNA sequencing. We’ve gone from sequencing the first genome for about $2.7 billion in the Human Genome Project just a few years ago and rather quickly come down to [more...]
Date: 2009-08-24 22:52:00
Thrombosis Journal | Full text | Expression of sterol regulatory ...
DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]
Date: 2009-02-18 08:00:00
Methylation Test May Aid Colon Cancer Diagnosis (CME/CE) | Discuss ...
To develop a more sensitive test for DNA methylation, investigators employed a form of technology known as BEAMing (beads, emulsion, amplification, and magnetics). They performed PCR amplification of individual DNA fragments covalently linked to magnetic beads suspended in water-based nanoparticles immersed in oil droplets. The beads contained a DNA sequence specific for exon 1 of the vimentin gene, which is hypermethylated in colon cancer. Following PCR amplification, the beads were hybridized by means of fluorescent probes specific to the state of methylation and then sorted and analyzed by [more...]
Date: 2010-10-09 11:00:27