Our blogs and videos choice: cell dna extraction

Information About Aminocentesis | I am pregnant!

Except for the traditional conventional blood sample, there are lots of methods utilizing a variety of sampling now available in profiling DNA for paternity identification. A single of these advanced systems is known as amniocentesis. It truly is in addition referred to as amniotic fluid test or AFT. Originally, amniocentesis is a prenatal method to diagnose the chromosomal abnormalities and fetal infections. The amniocentesis is performed inside the second trimester or throughout the 14th to 20th weeks of pregnancy. In this action, the OB-GYN uses ultrasound to guide a thin needle into the [more...]

Date: 2010-10-12 10:47:28

Blog posts (53) | Videos (26)

HPV DNA Test for Cervical Cancer

According to Lab Tests Online, the HPV DNA test still requires a sample of cells from the cervix. It is NOT a blood test. I emphasize this point because most of the news I’ve seen either got it wrong or didn’t clarify the testing procedure. For most people, DNA tests still mean a blood test even though DNA can be extracted from other samples, such as the ubiquitous cheek swab. Currently, the HPV DNA test is used in combination with the traditional pap smear. However, recent studies have shown that HPV testing may be used alone and make it possible for women to prolong the time between [more...]

Date: 2007-10-22 11:30:43

March 12, 2010: DNA EXTRACTION LAB

Students extracted DNA from wheat germ by using hot water, detergent, and isopropyl alcohol.  Hot water was used to denature DNase (an enzyme that cuts up DNA).   Detergent was used to remove the protein and lipids that make up the membrane of the cell.  This will help the cell as well the nucleus break apart, allowing the DNA to be released.  Detergent works well here, the same way it works to remove protein and fat off of your dishes at home.  Since DNA is not soluble in alcohol, the DNA will seperate from the solution and become [more...]

Date: 2010-03-12 20:09:34

Baby DNA test: Who is the father? Part II

A baby DNA test is one means of establishing with certainty who the daddy of the unborn child is. One can of course choose to wait till after the child is born although it is important to know that this is not necessary as a paternity test can be conclusively carried out while the mother is still pregnant. How is the Baby DNA testing done? There are two main methods through which the unborn baby’s DNA can be sampled. Amniocentesis: this is carried out anywhere between week 14 and week 20 of the pregnancy and is normally done as a routine procedure to locate abnormalities in the chromosomes. [more...]

Date: 2010-09-02 10:47:14

Tecan is first choice for quality and reliability

You are here: Home » News » Featured news » Tecan is first choice for quality and reliability Tecan is first choice for quality and reliability Publication date: 2 September 2010 Author: kdm communications limited Tagged with: Auto Q Biosciences, Kudrat Rahman, Regional Molecular Genetics Service (RMGS), Stuart Bayliss, Tecan The Regional Molecular Genetics Service (RMGS) at St Mary’s Hospital, Manchester, is using a Freedom EVO® liquid handling workstation in combination with chemagen’s proven nucleic isolation technology to perform fully automated DNA extraction. Supplied by [more...]

Date: 2010-09-02 16:21:24

The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...

There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]

Date: 2010-10-04 21:15:46

IVF Mail - single cell DNA Extraction

Hi all.. please can you tell me how we can extract DNA from single cell  for downstream amplification using real time PCR (PGD analysis). please if you know tell me the name of such kit used and the company manufacture it. really I am very interested in this [more...]

Date: 2010-10-04 22:33:19

geek speak - Bio-Synthesis, Inc. Begins Mitochondrial DNA Testing

Bio-Synthesis, Inc. begins Mitochondrial DNA Testing or MtDNA, making it one of only a handful of labs to perfect the technology. MtDNA Testing allows Forensic scientist to get a profile from samples that are old or possibly damaged, where the usual technique may not be effective. (Media-Newswire.com) - Recently Bio-Synthesis, Inc. has begun Mitochondrial or MtDNA Testing making it one of only 4 labs in the United States who have perfected the technology and offer it commercially. This expansion has allowed for more varied test options and opens the door to new industries that may benefit [more...]

Date: 2008-12-03 09:43:00

Basic Principles

Nuclear or genomic DNA is found in all nucleated cells as well as in the reproductive cells (eggs and sperm). The amount of DNA we can expect to find in different cells and types of evidence is found in Table 1. ... The second method is a specialized 'nonorganic' extraction using Chelex beads. Chelex beads can only be used when PCR-based DNA testing is going to be used. The basic DNA extraction procedures, whether organic or non-organic, can be adapted for special [more...]

Date: 2010-08-01 06:59:59

Discussion of Paternity DNA Testing | Forever Young

That is why, it is strongly suggested that people who are thinking about chorionic villus sampling or amniocentesis prenatal DNA paternity diagnostic tests to talk over with their obstetrician just before initiating the check. For other alternatives, a non-invasive paternity DNA testing known as fetal genetic substance assessment is accessible too. It is in response to the risks to fetus posed by earlier mentioned invasive techniques. The engineering used in fetal genetic material tests enables obstetricians to determine the paternity of an unborn little one via only maternal blood sample. [more...]

Date: 2010-10-13 16:15:06

DNA analysis – Using reagents?

The only problem is that when the cell's DNA is released from its protective nuclear membrane during extraction, the cell's DNA becomes exposed to its very own DNases. Some extraction protocols call for the use of special reagents that [more...]

Date: 2010-08-10 04:18:09

The Purification of Plasmid DNA for Clinical Trials Using Membrane ...

ABSTRACTMembrane chromatography offers a good solution to the challenge of developing an efficient chromatographic procedure for plasmid DNA purification. The large convective pores of anion exchange membranes allow plasmid DNA to access all the anionic binding sites of the membrane at high flow rates. Here we demonstrate that the pIDKE2 plasmid can be purified from a recombinant Escherichia coli lysate using a Sartobind D membrane combined with size exclusion chromatography to render material with 95% purity and an average yield of 50%. This process yields therapeutically suitable plasmid [more...]

Date: 2010-02-01 05:00:00

Freshman Biology: Biomolecules, Connecting to Evolution

Use Models to show DNA. 4.) Utah Cell Scale Interactive to show the different organic biomolecules. Thursday 10/14: SWBAT: Describe the four major categories of organic polymers. 1.) Finish Vocabulary from Wednesday. 2.) Chlorophyll Extraction Lab. 3.) Walking to the orchard? Monday 10/18: SWBAT: Describe the four major categories of organic polymers. 1.) Around the room classification of biomolecules. 2.) Pyramid with Biomolecules thrown in. 3.) DNA building as two teams. 4.) Review cell scale on Utah website. Tuesday 10/19: SWBAT: Analyze DNA sequences to determine evolutionary [more...]

Date: 2010-10-12 11:31:00

DNA Testing During Pregnancy

The cool thing is that DNA testing during pregnancy can make such determinations during pregnancy. This can be done by blood or saliva tests after the child is born, but before the child is born, the procedure is a little more complicated. A paternity during pregnancy must be done by a process called an amniocentesis. What is Amniocentesis? With an amniocentesis, the paternity test before birth is done on the amniotic fluid. This can take place between 14 and 20 weeks of pregnancy. With the guidance of an ultrasound, the physician will insert a needle into the uterus and into the amniotic [more...]

Date: 2010-07-03 10:11:55

Strawberry DNA Extraction | Serendip's Exchange

DNA Isolation using Strawberries (wear eye protection, or be careful).  Working in groups (for supervision), but each individual will do their own. Place 1 strawberry in the plastic bag.  Remove air and seal. Mash strawberry for 2 minutes. Add ~10 ml DNA Extraction buffer (Pre-measured).  Remove air and seal. Mash for 1 minute. Pour solution through coffee filter  into paper cup Pour ~2 ml of this solution back into the 15 ml tube.  It will be very red. Pour ~ 4 ml (2X the volume of strawberry solution) of cold ethanol (found on freeze door in 50 ml tubes) so that it lays on top of [more...]

Date: 2008-07-30 07:00:00

30-min DNA isolation protocol for filamentous fungi

Rapid and efficient protocol for DNA extraction and molecular … DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption 30-min DNA isolation protocol for filamentous fungi Be the first to comment - What do you think? Posted by admin - March 21, 2010 at 5:07 pm Categories: DNA ISOLATION, Diversity, Dna, Fungi  Tags: 30-min, DNA ISOLATION, filamentous, Fungi, [more...]

Date: 2010-03-22 01:07:05

DIY DNA extraction, Royal Society, and maps.

Having donned white coat and purple gloves we then spent over an hour extracting our DNA from some cheek cells using surfactants and alcohol, Pasteur pipettes and micro pipettes. I was too successful, in that I produced such a big glob [more...]

Date: 2010-09-08 19:57:50

Company Profile : Diagnostic supplier : Suyog Diagnostics : Mumbai ...

The company is representing world renowned Diagnostics Companies and selling Diagnostics Kits for Coagulation disorders, Viral Markers for HIV, HBV, HCV, HDV, HEV, Biochemistry, Chromatography kits etc. Life Science Division: The company is representing world renowned Diagnostics Companies and selling Chemicals, Bio - Chemicals, FISH Probes, Radio Chemicals, Reagents, Cell Bio, Electrophoresis grade, Neuro chemicals, Molecular Biology grade, DNA Extraction kits, PCR Kits, Restriction Enzymes, DNA Ladders & modifying enzymes, Proteomics, Cell Culture reagents, Plant Tissue Culture Grade [more...]

Date: 2010-07-09 15:29:00

Strawberry DNA Extraction Lab

Why do we use the dishwashing liquid and meat tenderizer at the beginning of the DNA extraction process?  (Hint: think cell membranes!) Why did we add rubbing alcohol at the end? Explain. (HINT: Look up the words: SOLUBLE and [more...]

Date: 2010-09-30 15:36:53

Odyssey moving slowly toward the dock; Whale samples being ...

Next, we will take a drop of DNA from each whale and put it in a special plate and send it to our collaborator at Oregon State. His team will then perform a related technique that will provide a DNA fingerprint for each whale. This information will identify each whale uniquely and show familial relationships. Then we may be able to separate our whales into families and see if we sampled any whales twice. The DNA extractions started today so that work is in process. In addition, James continues his work on chemical dispersant toxicity in human cells, while Cathy ramps up her efforts of [more...]

Date: 2010-09-30 21:19:00

Noticias de Salud: Tecan is first choice for quality and reliability

Un blog con noticias de actualidad relacionadas con el mundo de la salud. Novedades, encuestas, estudios, informes, entrevistas. Con un sencillo lenguaje dirigido a todo el mundo. Y algunos consejos turísticos para pasarlo bien The Regional Molecular Genetics Service (RMGS) at St Mary’s Hospital, Manchester, is using a Freedom EVO® liquid handling workstation in combination with chemagen’s proven nucleic isolation technology to perform fully automated DNA extraction. Supplied by chemagen’s UK distributor, Auto Q Biosciences, this integrated system is housed within a Class 1 biological [more...]

Date: 2010-09-02 16:25:00

Science Fair Project: DNA Extraction

Step 1. Topic. When doing a science fair project, your first step is to select a topic. You have selected the topic DNA. Since a science fair project should be an experiment, I recommend extracting DNA from plant or animal cells. [more...]

Date: 2010-05-11 22:20:06

Research Assistant II - OHSU Human Resources

This is a research position involving cell culture, RNA and DNA extraction, PCR, Western and Northern blots and other advanced molecular biology techniques. Incumbent will attend weekly labmeetings and present research upon request. [more...]

Date: 2010-09-30 08:00:00

Lab 8: Strawberry DNA Extraction (Sat).

o Challenge students to form connections between biology, global issues and local impacts. o Raise students’ awareness about bioethics and biological careers. o Further students’ interest in biology. This course is designed for students to develop an inquiry point of view toward living systems in the context of a changing environment. Laboratory experiments heighten and integrate events at the molecular, cellular and population levels of organization. Specific topics include cell structure and function, biochemical mechanisms, and principles of genetics and evolution. Independent reading [more...]

Date: 2009-01-10 13:25:00

Nonhomologous DNA End Joining in Cell-Free Extracts

The authors proposed three independent mechanisms for the end-joining based on the sequence at the junctions, which were single-stranded DNA ligation, template-directed ligation, and postrepair ligation . Later, other studies helped [more...]

Date: 2010-08-07 07:00:00

DNA: Size Does Matter. But is it Science or Art? « Exploratorium ...

Then I randomly found this website, which starts with the same idea as our DNA Cheek Cell Extraction Demo but takes it to a whole new level:  You send your cheek cells in the mail to their company.  They extract your DNA from your cells, then use PCR to amplify unique sections of DNA in your genome.  This DNA is loaded into a gel and a current is run through the gel to separate the DNA based on size.  UV dye is added to the whole thing to make your DNA strands visible, a photograph is taken, and voila!…a giant canvas print with the color scheme of your choice is ready to hang on your [more...]

Date: 2008-05-14 07:37:47

5 minute DNA Extraction in a Shot Glass

A blog that offers research to help you achieve and maintain health… "If we don't play God, who will?" – James Watson Intro: 5 minute DNA Extraction in a Shot Glass Despite its exotic-sounding name, DNA is ubiquitous – it can be found in every cell of every living thing and almost everywhere on the planet. Nonetheless, we rarely come face-to-face with the molecule itself – and it’s not because DNA is difficult to find or isolate! In this instructable, we’ll show you how to isolate your own DNA with little more than some dish soap, table salt, high-proof alcohol, a shot glass, and [more...]

Date: 2009-11-13 04:41:42

Plasmid v Genomic DNA Extraction:The Difference | Bitesize Bio

If you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in [more...]

Date: 2009-04-16 08:49:09

Research Progress Report I

Without the qPCR results, it is hard to say which clean-up procedure is better, but it seems that both have about the same yield of DNA. There was not enough DNA in the PC+Q sample to run a qPCR. Another thing to consider is that this experiment was performed with a Qiagen gel extraction kit, not a kit designed specifically for DNA clean-up. Using such a kit (they do have one) may possibly yield better results. Future Experiments: I am currently growing up 250mL worth of AFT1 cells for my next ChIP experiment. I will probably be comparing the old bead beater with the new bead [more...]

Date: 2010-10-04 01:35:29

BioStorage adds lab sample preparation services

Following sample processing BioStorage allocates aliquots of blood or DNA for use in future testing. BioStorage uses its tracking and inventory management system, ISISS, for informed sample prioritisation. By moving into sample preparation services BioStorage can help clients mitigate the risk of variables that can impact on sample integrity. Taking control of these aspects of sample management helps BioStorage ensure quality of samples it ships for laboratory testing. Furthermore, BioStorage believes the additional services will ultimately lower costs for clients, in part by streamlining [more...]

Date: 2010-10-13 09:00:00

Plasmid DNA Extraction Kit

DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the environment as well as diagnosing disease and genetic disorders. The GenQuik™ Plasmid DNA Extraction Kit, utilizing an exclusive membrane technology in the form of convenient spin columns and 96 well plates, makes the isolation of high quality plasmid DNA from recombinant E.coli cultures simple, rapid and cost-effective. For Details on GenQuick ™ Plasmid DNA [more...]

Date: 2010-07-29 17:42:05

Isolation of active constituents of ACORUS CALAMUS RHIZOME Extract ...

Isolation of active constituents of ACORUS CALAMUS RHIZOME Extract and Evaluation of its Anti-Cancer Activity. The Molecular Basis of Cancer and By-products of Tumor Cells. The process of carcinogenesis is the result of DNA damage that [more...]

Date: 2010-10-13 07:16:43

TopBP1 and DNA polymerase-α directly recruit the 9-1-1 complex to ...

TopBP1 and DNA polymerase-α directly recruit the 9-1-1 complex to stalled DNA replication forks Shan Yan and W. Matthew Michael The Biological Laboratories, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138 Correspondence to W. Matthew Michael: mmichael{at}fas.harvard.edu Abstract TopBP1 and the Rad9–Rad1–Hus1 (9-1-1) complex activate the ataxia telangiectasia mutated and Rad3-related (ATR) protein kinase at stalled replication forks. ATR is recruited to stalled forks through its binding partner, ATR-interacting protein (ATRIP); however, it is unclear [more...]

Date: 2009-03-23 07:00:00

University researchers working to perfect micro DNA technology ...

University of Virginia researchers are working to develop “lab-on-a-chip,” which could drastically change such scenarios by shrinking technology that requires time, laboratories full of people and expensive equipment into a glass slide smaller than a credit card. Such technology has been promised for close to two decades, warns James Landers, a University of Virginia professor of chemistry and mechanical engineering and an associate professor of pathology. He is the lead researcher for the lab- on-a-chip project. While he is cautious about the “unknowns” yet to be dealt with, Landers [more...]

Date: 2008-11-14 05:01:00

"Happy and Healthy" A Cancer Free Living Blog: Bone Marrow ...

Go to http://www.marrow.org/, fill out the online registration form and get your at home registration kit within a few weeks. Once you receive the kit, follow the simple directions and send back your DNA sample. The sample will then be analyzed and the results are compared to potential recipients. Like myself you may of heard "bone marrow transplant" and had visions of long needles and painful procedures, but getting registered is nothing more than a cheek swab. That's right, a cotton swab to the inside of the cheek. Nothing more than what you see for a DNA test on TV. If you're a match for [more...]

Date: 2010-10-12 19:55:00

Biomarkers in oropharyngeal squamous cell carcinoma

Oropharyngeal squamous cell carcinomas (OSCC) constitute about 5% of all cancers in the western world and the incidence and mortality rates of this tumor have shown little improvement over the last 30 years.  Molecular targeted therapy, a promising strategy for the treatment of OSCC and other cancers, requires the understanding of specific molecular events of carcinogenesis and the different pathological, partly interrelated pathways. Extended knowledge of the prognostic or predictive value of molecular biomarkers in oropharyngeal cancer is necessary to allow a better characterization and [more...]

Date: 2010-09-13 09:32:00

DNA Extraction from Mouse Tail

NOTICE: YOU NEED TO FILL YOUR PROFILE PROPERLY TO BE ABLE TO DOWNLOAD VIDEO. LOOK BELOW TO SEE THE NECESSARY INFORMATION TO BE FILLED. Your acedemic background information is missing. Please click here to fill this information. Your occupation information is missing. Please click here to fill this information. Your current school and company information is missing. Please click here to fill this information. Your current "about me" information is missing. Please click here to fill this [more...]

Date: 2010-08-30 19:33:07

RNA/DNA extraction

RNA/DNA extraction follows simple principles: lysis of cells containing DNA/RNA; wash cell debris; removing proteins with a protease; precipitating the DNA/RNA with an alcohol; resuspension/elution of DNA/RNA [more...]

Date: 2010-09-03 11:56:44

Antibody Purification Using Membrane Adsorbers - Results from a ...

Dec 1, 2008 By: Leonardo Giovannoni, PhD, Marco Ventani, Uwe Gottschalk, PhD BioPharm International Volume 21, Issue 12 ABSTRACT Most antibody manufacturers currently use a three-column platform comprising Protein A affinity chromatography for product capture, followed by anion exchange (AEX) chromatography in flow-through mode to extract negatively charged contaminants, and then cation exchange (CEX) chromatography or hydrophobic interaction chromatography (HIC) in retention mode to remove positively charged contaminant species. This article presents a new process that uses membrane [more...]

Date: 2008-12-01 05:00:00

SciWorks to Host BioTechnology Days 9/23/10 and 9/24/10

Winston-Salem, NC: The future of the Piedmont Triad resides in science education and the growth of the biotech industry. As part of the NC Science Festival and in response to the increased need for science education, SciWorks Science Center is partnering with the NC BioNetwork and Connect a Million Minds, an initiative of Time Warner Cable, to provide hands-on activities that will show how biotechnology is part of our every day lives. Also participating is the Center for Excellence in Research, Teaching and Learning (CERTL) of Wake Forest University Baptist Medical Center. Middle School [more...]

Date: 2010-09-21 12:00:00

At Home Paternity Test | Prenatal Paternity Test

The convenience of these at home tests is great as well.  Once the test comes to your home, you can use it at your convenience, and the mail it back in the free packaging that comes with the test.   Once you’ve mailed it back, the results will be sent to you within 3-5 working days.  Sometimes it will take a bit longer if the samples are difficult, but for the most part, you will receive the results quickly. Basically, the paternity tests can determine parentage of a child by genetic testing.  When you swab the inside of the cheek, it scrapes off skin cells that contain DNA.  These [more...]

Date: 2010-07-06 03:54:50

Top ten most accessed articles in August

Cell lysis and DNA extraction of gram-positive and gram-negative bacteria from whole blood in a disposable microfluidic chip  Madhumita Mahalanabis, Hussam Al-Muayad, M. Dominika Kulinski, Dave Altman and Catherine M. Klapperich  Lab Chip, 2009, 9, 2811 – 2817, DOI: 10.1039/b905065p  Programmable diagnostic devices made from paper and tape  Andres W. Martinez, Scott T. Phillips, Zhihong Nie, Chao-Min Cheng, Emanuel Carrilho, Benjamin J. Wiley and George M. Whitesides  Lab Chip, 2010, 10, 2499 – 2504, DOI: 10.1039/c0lc00021c  Microfluidics without pumps: reinventing the [more...]

Date: 2010-09-28 08:45:53

Prenatal Paternity Tests

The situation may arise where it becomes necessary to find out the baby’s paternity prior to them being born. Where this is the case prenatal Paternity Testing can be done. This test has to be done by a well trained doctor. There is currently no home testing that is available for prenatal testing. It is very important that a doctor is involved throughout the process because there are a number of factors that influences the foetuses health. So even though it may be important to the mother to determine paternity, the doctor will always consider the health of the child first. Also a prenatal [more...]

Date: 2010-08-29 20:02:31

Cytogenetics Clinical Lab Technologist Job / Mayo Clinic ...

Knowledge of genetics, molecular genetics, DNA extraction, PCR, microscopy, cell culture and aseptic technique is desirable. Experience with operation and troubleshooting of laboratory instrumentation and assay systems is desirable. CV/RESUME, COVER LETTER, and TRANSCRIPTS, unofficial copy accepted, MUST ALL BE INCLUDED FOR YOUR APPLICATION TO BE CONSIDERED. **A 2 YEAR COMMITMENT TO THIS POSITION IS REQUIRED. Benefit Eligibility: Yes Posting Begin Date: 08/06/2010 Posting End Date: Until Filled Employment Type: Non Exempt Hours/2-Weeks: 80 Schedule: Monday - Thursday Shift: [more...]

Date: 2010-10-09 21:08:40

Fayette Life: Fruitful DNA Extraction

What do strawberries and bananas have in common with Fayette County's Whitewater Middle School students? The answer is DNA. As it turns out, the genetic material of the fruits is very similar to that of humans. This was just one fascinating fact that approximately 290 seventh graders learned as they participated in a DNA extraction lab conducted by graduate students from the Georgia Plant Scholars Program of the University of Georgia’s (UGA) Plant Pathology Department. Using regular household items such as coffee filters, salt, dishwashing liquid, water and rubbing alcohol, students learned [more...]

Date: 2009-05-27 12:12:00

Extracting DNA from Your Cells

1 DNA Based on and adapted from the Genetic Science Learning Center's “How to Extract DNA from Any Living Thing” (learn_genetics_utah_edu/units/activities/extraction/) and BioRad's “Genes in a bottle” ... Adding the detergent to you cheek cell solution will break open the cell membranes and nuclear membranes and release your DNA into the solution. Step 2: Enzymes Add a pinch of enzyme (meat tenderizer) to your test tube. With your gloved thumb (or palm) covering the top [more...]

Date: 2010-04-15 13:51:06

Research Technician: Cell Culture (Cambridge)

We're seeking a research technician with strong mammalian cell culture skills. Responsibilities include embryonic stem cell culture work, maintaining colonies, DNA extractions, some occasional animal work, good record keeping is a must. Will need to be available occasional weekends for 3 hours of animal work. Requires a BS with solid independent research experience and/or up to 1 year of industry experience. Commonwealth Sciences, Inc. is a professional recruiting firm specializing in Science, Engineering and Green Technologies. We unite clients with top industry professionals by leveraging [more...]

Date: 2010-10-07 02:26:11

Part-time Lab Assistant-Tissue culture (Sorrento Valley)

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Sorrento Valley biotech start-up seeks a part-time Lab Assistant to be responsible for mammalian cell culture / cell line generation. Work hours: 15-25 hr/week Job duties: • Stable mammalian cell line generation • Mammalian suspension cell culture • Expression vector cloning including transformation, ligation, DNA gel extraction, and mini/midi prep • Protein purification and analysis work as needed • General lab maintenance Requirements: • BA with 2-3 years of experience • Strong [more...]

Date: 2010-04-02 03:24:50

DNA Isolation and Modeling « NWABR

This entertaining and inexpensive protocol was adapted from Biotechnology in the Classroom, UC Davis and Diane Sweeney, Biology — Exploring Life. First we, the teacher-students line up for our materials: Dr. Hutchison regales us as we select strawberries and collect materials: This flexible protocol has been used with first graders all the way through high school and administrators. You can make it as in depth as you like. All materials can be bought in a grocery store, there are no expensive reagents. This was my lovely strawberry, in a sealed plastic bag and on a paper towel, [more...]

Date: 2010-03-07 00:00:57

DNA Extraction

We first need to collect cell from the test subject. The skin inside of our mouths losses thousands of cells everyday. These cells are ideal for DNA Extraction. Once the “Cheek Cells” are collected you will need to place the swab used [more...]

Date: 2009-04-03 06:38:12

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