Our blogs and videos choice: automated dna sequencing

Nobel season '10: Chemistry

I've also seen that different large-scale DNA-sequencing approaches, primarily shotgun sequencing, are on the map in Swedish media which I think is really strange. Firstly because Fred Sanger, who developed shotgun sequencing, is 92 years old and a double Nobel laureate in chemistry already (1958 and 1980), and secondly because the field of large-scale sequencing still hasn't peaked by any means. Perhaps Leroy Hood (again) could be considered for the development of the first automated DNA sequencer? Anyway, the technologies that are advancing the field have been developed by private companies [more...]

Date: 2010-10-06 07:00:00

Blog posts (23) | Videos (25)

DNA sequencing » Blog Archive » DNA Sequencing Methods

DNA Sequencing – How DNA Sequencing Works – Methods for … An explanation of how DNA sequencing works. … Typically, the automated sequencing method is only accurate for sequences up to a maximum of about 700-800 base-pairs in length. … DNA Sequencing Simulation (Sanger Method) Within each reaction mixture are the following items: a DNA segment of unknown … The other DNA sequencing method (named for Allan Maxam and Walter … Next-Generation DNA Sequencing Methods 921k – Adobe PDF – View as html generation DNA sequencing technologies highlight the striking impact … [more...]

Date: 2010-09-28 04:29:58

"Polymerase Chain Reaction (PCR)" Biology Animation Library ...

"Polymerase Chain Reaction (PCR)" Biology Animation Library :: Dolan DNA Learning Center: "Polymerase Chain Reaction Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA. This animation is featured in our 'Spotlight Collection' on Polymerase Chain Reaction, along with video interviews with Kary Mullis, a 3D molecular animation of PCR, and several laboratory [more...]

Date: 2010-08-23 17:54:00

The Picasso of DNA

Church thrived in Gilbert's lab. In 1977 he developed a way of automating a key step of Gilbert's DNA sequencing method. DNA strands are made up of combinations of four bases, molecules that are denoted by the letters A, T, C, and G. [more...]

Date: 2010-07-22 20:46:05

PLoS ONE: Whole-Genome Sequencing and Assembly with High ...

In this paper we describe SHRAP (SHort Read Assembly Protocol), a sequencing protocol and assembly methodology designed to assemble a complex mammalian genome with high fidelity using short reads from such technologies. ...... (1990) Automated DNA sequencing of the human HPRT locus. Genomics 6: 593–608. Find this article online; Roach JC, Boysen C, Wang K, Hood L (1995) Pairwise end sequencing: a unified approach to genomic mapping and sequencing. Genomics 26: 345–353. [more...]

Date: 2007-05-30 00:00:00

Quick & Reliable DNA Purification and Sequencing…

BioCel DNA Purification and Sequencing Royston, UK -- An applications bulletin is available from Agilent Automation Solutions that describes an automated system that provides for quick and reliable DNA purification and sequencing. The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems. Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting [more...]

Date: 2010-08-14 14:00:00

The Foundations of P4 Medicine | P4 Medicine at Ohio State

For example, automated DNA sequencing is now moving into a third technology generation. I predict that in less than 5 years we will be able to sequence an individual's genome for under $1000. Thus, your genome sequence will soon become [more...]

Date: 2010-10-13 16:19:51

Efavirenz Plasma Concentrations and Cytochrome 2B6 Polymorphisms ...

Genomic DNA was extracted from the peripheral whole blood of each subject with a DNA extraction kit (Qiagen, Valencia, CA). CYP2B6 polymorphisms were genotyped by resequencing using primers and polymerase chain reaction (PCR) conditions described by Lamba and associates.15 Sequencing was carried out with an ABI Prism 3700 Automated Sequencer using the PCR primers or internal primers (sequence available on request). Sequences were assembled using the Phred-Phrap-Consed [more...]

Date: 2010-09-22 19:28:38

Research assistant, DNA hash pooling | MetaFilter Jobs

... project (www.nanoinfobio.org), the appointed Research Assistant will be responsible for experimental validation of this technique using molecular biology methodologies such as DNA extraction, PCR, RFLP and automated sequencing. [more...]

Date: 2010-08-06 00:24:19

Doe Contractor-grantee Workshop Sequencing Technologies

A bottleneck in gel-based systems is the manual gel-preparation step, which is both time consuming and a potential source of variability in DNA sequencing. Barry Karger (Northeastern University) discussed a fully automated, closed-end, [more...]

Date: 2010-07-05 19:44:31


GeneDesign automates the process of determining which base pairs -- the building blocks of DNA -- should be linked together in a particular order to make a gene, according to Jef Boeke, Ph.D., professor of molecular biology and genetics and ... the tasks required to design and manipulate synthetic DNA sequences. The program allows the user to start with either the sequence of the amino acid making up the protein or the bases making up the gene that codes for that protein. [more...]

Date: 2006-02-15 08:00:00

DNA sequencing « Lenovo Laptop

Cognition of DNA sequences has gone indispensable for basic biological research, other inquiry legs utilizing DNA sequencing, and in numerous Gene Expression applied fields such as diagnostic, biotechnology, forensic biology and biological systematics. The advent of DNA sequencing has significantly accelerated biological inquiry and discovery. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of the human genome, in the Human Genome Project. Related projects, frequently by scientific collaboration across continents, receive [more...]

Date: 2010-10-12 16:36:19

The Spittoon » My $0.02 about The $1000 Genome

Although Davies generally appears to be a proponent of consumer genomics, he also provides examples of the possible risks of widespread DNA testing in addition to the benefits. While reading this book, I found strong parallels between Davies’ general themes and 23andMe’s overall philosophy. Both Davies and 23andMe stress the importance of “empowering the individual” and “democratizing genetics”.  Direct-to-consumer genetic testing puts the power of knowledge into the hands of the individual, in contrast to what is often deemed the paternalistic nature of the medical [more...]

Date: 2010-10-07 22:16:18

Quick and reliable DNA purification and sequencing

Keep up with the latest research, events and funding news. Add the  observer,  dissemination and  funding widget to your website or blog The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems.  Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting Station, a high-speed precision pipettor. With its versatile shelf configuration options, the [more...]

Date: 2010-07-28 13:03:49

dna synthesizer

Complete DNA Sequencing & Synthesis Services,DNA Sequencing Analysis System. Custom DNA Synthesis Get the synthetic advantage. Guaranteed Expression available. AltaVista found 823,000 results . DNA/RNA- Synthesizer – DNA/RNA-Synthesizer DNA/RNA Synthesizers. Oligo-Purifiers. Laboratories. Partners. Company  We are the future of DNA/RNA synthesis. K&A Laborgeraete. Impressum | Druckversion. DNA / RNA Oligonucleotide Synthesizer: MerMade Whether your DNA or RNA synthesizer requirements are a few custom oligos a week or several thousand a day we have a MerMade that will fit your [more...]

Date: 2010-07-11 06:58:31

Official launch of iBOL activates world's largest biodiversity ...

Toronto – The largest biodiversity genomics initiative ever undertaken – an international effort to build a digital identification system for all life on Earth – will be officially activated this week. The International Barcode of Life Project (iBOL) will be launched by the Minister of Research and Innovation, Glen Murray, during an event at Toronto’s CN Tower on Saturday, September 25 at 6:30 p.m. By enabling automated, rapid and inexpensive species identifications, iBOL will transform biodiversity science and its applications throughout society. “We are witnessing alarming rates [more...]

Date: 2010-09-24 13:22:46

dna sequencing

DNA sequencing reactions all use a primer to initiate DNA synthesis.  A Quicktime animation of how dideoxy nucleotides are incorporated into DNA in sequencing reactions. Nucleic acid sequence This sequence is written as a succession of letters representing a real or hypothetical DNA molecule or Sequences can be read from the biological raw material through DNA . DNA Sequencing – Real Time DNA analysis Information With the latest chellenges in DNA Sequencing, our organization looks to unravel the myths, truths and most essential assets in the deconstruction of genes. DNA Sequencing The [more...]

Date: 2010-07-09 03:20:29

CN Tower to Double as DNA Barcode

25 to look like the world's biggest DNA barcode as part of the official launch of the University of Guelph-based International Barcode of Life Project (iBOL). iBOL is the world’s largest biodiversity genomics initiative aimed at creating a digital identification system of all life on Earth using DNA barcoding. The international project will be launched by Glen Murray, Ontario’s minister of research and innovation, at the CN Tower at 6:30 p.m. University of Guelph president Alastair Summerlee and iBOI scientific director Paul Hebert, a professor in the University’s Department of [more...]

Date: 2010-09-24 13:58:11

automated dna sequencing

Fluorescence-based DNA sequencing systems use dideoxy chain  A text file of the inferred DNA sequence is output directly. Automated DNA Sequencing Guidelines: One of the most common problems encountered in automated DNA sequencing  DNA sequences will be available in 2-4 days after they are submitted. Automated DNA Sequencing Request Automated DNA Sequencing Request. Date: Requested By: Principal Investigator: P.O. DNA SEQUENCING. – See website for volume discount on BigDye XTerminator sequencing . Automated DNA Sequencing – Biocompare Automated DNA Sequencing DNA Sequencing | [more...]

Date: 2010-07-09 06:51:50

Genomics: The next generation | Wellcome Trust

The advent of the Human Genome Project and the development of faster, cheaper DNA sequencing revolutionised medical and biological research - and the technology is still evolving. Mun-Keat Looi looks at the platforms pushing us closer toward the ‘$1000 genome’. At the turn of the millennium, genomics captured the public's imagination with the publication of the first draft of the human genome and the insights it offered into our biology. When the Human Genome Project started in 1991, DNA sequencing entailed laborious radiation-based methods, with researchers manually loading [more...]

Date: 2009-07-28 11:00:00

FinchTalk: From Reads to Data Sets, Why Next Gen is not like ...

The first widely-used sequencing systems were based on the “Sanger” method. DNA was synthesized in the presence of chain terminating radioactive dideoxy-nucleotides (1). Mixtures of DNA fragments were separated by size using gel electrophoresis and the bases were identified and entered into a computer through manual techniques. Automated DNA sequencing instruments arrived later. These instruments made DNA sequencing thousands of times more efficient by detecting fluorescently labeled fragments and sending the information directly to a computer (2). For the first time, it became possible [more...]

Date: 2009-01-06 19:16:00

HHMI News: Timothy Harris to Deliver Public Talk at Janelia Farm

The event is free and open to the public, but tickets are required for admission. Directions for obtaining the tickets are available on the HHMI web site at www.hhmi.org/janelia/events.html. Seating is limited to 250 people. The lecture is the seventh in a series called “Dialogues of Discovery at Janelia Farm.” Past speakers in the series have included Thomas R. Cech, former president of HHMI and an HHMI investigator at the University of Colorado, Boulder; Roian Egnor, a fellow at the Janelia Farm Research Campus; Ronald M. Evans, an HHMI investigator at the Salk Institute for Biological [more...]

Date: 2010-10-04 17:01:00

Bioinformatics Programmers Require For Scigenom Labs Pvt Ltd /M.Sc ...

We conduct research work aimed at understanding the role of DNA sequence variation in human health and disease. Our state of the art DNA sequencing laboratory providing Sanger sequencing, analysis and genotyping service to research institutions, investigators and laboratories worldwide. Name of the Position : Bioinformatics Programmers Number of Opening : one Name of the Institute : Scigenom Labs Pvt Ltd Functional Area :Application Programming, Maintenance Experience: 1 - 5 Years Location:Ernakulam / Kochi/ Cochin Desired Candidate Profile : The person in this position should have good [more...]

Date: 2010-08-31 18:47:29

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