article - Aspergillus PCR formidable challenges and progress
lyase which might be contaminated by fungal DNA. . For DNA extraction, either spin columns or an automated method can be performed. Sources of contamination during fungal DNA extraction may also include buffers and spin columns (the [more...]
Date: 2009-04-08 07:00:00
Tecan is first choice for quality and reliability
You are here: Home » News » Featured news » Tecan is first choice for quality and reliability Tecan is first choice for quality and reliability Publication date: 2 September 2010 Author: kdm communications limited Tagged with: Auto Q Biosciences, Kudrat Rahman, Regional Molecular Genetics Service (RMGS), Stuart Bayliss, Tecan The Regional Molecular Genetics Service (RMGS) at St Mary’s Hospital, Manchester, is using a Freedom EVO® liquid handling workstation in combination with chemagen’s proven nucleic isolation technology to perform fully automated DNA extraction. Supplied by [more...]
Date: 2010-09-02 16:21:24
Efavirenz Plasma Concentrations and Cytochrome 2B6 Polymorphisms ...
Genomic DNA was extracted from the peripheral whole blood of each subject with a DNA extraction kit (Qiagen, Valencia, CA). CYP2B6 polymorphisms were genotyped by resequencing using primers and polymerase chain reaction (PCR) conditions described by Lamba and associates.15 Sequencing was carried out with an ABI Prism 3700 Automated Sequencer using the PCR primers or internal primers (sequence available on request). Sequences were assembled using the Phred-Phrap-Consed [more...]
Date: 2010-09-22 19:28:38
Research assistant, DNA hash pooling | MetaFilter Jobs
... project (www.nanoinfobio.org), the appointed Research Assistant will be responsible for experimental validation of this technique using molecular biology methodologies such as DNA extraction, PCR, RFLP and automated sequencing. [more...]
Date: 2010-08-06 00:24:19
Company Profile : Diagnostic supplier : Suyog Diagnostics : Mumbai ...
The company is representing world renowned Diagnostics Companies and selling Diagnostics Kits for Coagulation disorders, Viral Markers for HIV, HBV, HCV, HDV, HEV, Biochemistry, Chromatography kits etc. Life Science Division: The company is representing world renowned Diagnostics Companies and selling Chemicals, Bio - Chemicals, FISH Probes, Radio Chemicals, Reagents, Cell Bio, Electrophoresis grade, Neuro chemicals, Molecular Biology grade, DNA Extraction kits, PCR Kits, Restriction Enzymes, DNA Ladders & modifying enzymes, Proteomics, Cell Culture reagents, Plant Tissue Culture Grade [more...]
Date: 2010-07-09 15:29:00
تقنية البلمرة الجزيئية » تعرف عن عملية استخلاص شريط الحمض النووي ...
The VX Viral DNA/RNA Purification CorProtocol™ 25101 allows for walk-away automated preparation of low copy viral DNA/RNA from low total nucleic acid-yielding samples. Extracted viral DNA/RNA is of high quality and suitable for a wide variety of downstream applications. The default sample volume is 200 µL but can be scaled up to 500 µL with the use of adjustments to this protocol coupled with an alternative run file ( VX_RNA_DNA_CorProtocol_250_500ul_25101_06.CAS4 ) on the instrument. Increased volumes of certain reagents are required for the extraction of sample volumes greater [more...]
Date: 2010-07-09 16:20:26
Virology Journal | Full text | Molecular epidemiology of sexually ...
DNA was successfully extracted from a limited scraping of externally visible EGW using an automated DNA extraction system. The potential falsenegative rate for this study was 12% (13/105). A possible explanation is that insufficient DNA was present to generate an amplicon or the MY09-11 primer set, used in the initial screening for HPV positivity, may be sub-optimal for specific genotypes. With regard to this latter argument, it should be noted that a total of 15 out of a possible 17 high or probably high risk genotypes were detected with this methodological approach. Of the panel of high or [more...]
Date: 2009-08-09 07:00:00
DNA Testing 101
The swabs are then deposited into paper envelopes, which allow the sample to dry and preserve the DNA. The sample envelopes are handled only by authorized personnel during the transportation to the laboratory where the DNA extraction and analysis will take place. Initiating DNA Extraction In the laboratory, every step of the sample processing is done under strict security measures and according to the indicated protocol, in order to avoid contaminations or any other inconvenience. All the documentation is verified assuring that the information on the envelopes is clearly legible and accurate. [more...]
Date: 2010-04-30 07:30:00
Abbott Receives FDA Approval for First Automated Molecular Test ...
It is the first and only approved test capable of automating HBV viral load testing from sample extraction to final results. The Abbott RealTime HBV assay, based on real-time PCR (polymerase chain reaction) technology, is now available for laboratories that use the Abbott m2000 automated instrument system for molecular diagnostic testing. The test offers sensitive measurement (quantitation) of HBV in human plasma or serum from individuals chronically infected with HBV. The assay is intended for use as an aid in the management of patients with chronic HBV infection undergoing anti-viral [more...]
Date: 2010-09-01 05:00:00
Evaluation of a nested PCR targeting IS6110 of Mycobacterium ...
Blood culture was done in a completely automated blood culture system, BacT/Alert 3D® (Biomιrieux, NC, USA) according to the manufacturer's instructions. The blood culture results were communicated to the treating physicians in real time. Buffy coat preparation and DNA extraction Buffy coat was prepared from the third fraction of blood as indicated above. DNA was extracted in batches using QiaAmp blood mini kit (Qiagen GmBH, Hilden, Germany) as per the manufacturer's instructions. The extracted DNA was stored at -20°C and used for nPCR testing. PCR testing by targeting IS6110 sequence [more...]
Date: 2010-10-11 18:24:39
BioStorage adds lab sample preparation services
Following sample processing BioStorage allocates aliquots of blood or DNA for use in future testing. BioStorage uses its tracking and inventory management system, ISISS, for informed sample prioritisation. By moving into sample preparation services BioStorage can help clients mitigate the risk of variables that can impact on sample integrity. Taking control of these aspects of sample management helps BioStorage ensure quality of samples it ships for laboratory testing. Furthermore, BioStorage believes the additional services will ultimately lower costs for clients, in part by streamlining [more...]
Date: 2010-10-13 09:00:00
The prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae ...
Conclusions Prevalence of N. gonorrhoeae and C. trachomatis was detected to be low in Turkish women, and the difference between the groups was not significant. Both agents were more prevalent in subjects over 25 years of age. Content Type Journal ArticleDOI 10.1007/s00404-010-1694-8Authors Fahriye Eksi, Department of Medical Microbiology, Gaziantep University, Faculty of Medicine, Universite Bulvari, Gaziantep, 27310 TurkeyEbru Dikensoy, Department of Obstetrics and Gynecology, Gaziantep University, Faculty of Medicine, Gaziantep, TurkeyEfgan Dogan Gayyurhan, Department of Medical [more...]
Date: 2010-09-26 20:23:31
Noticias de Salud: Tecan is first choice for quality and reliability
Un blog con noticias de actualidad relacionadas con el mundo de la salud. Novedades, encuestas, estudios, informes, entrevistas. Con un sencillo lenguaje dirigido a todo el mundo. Y algunos consejos turísticos para pasarlo bien The Regional Molecular Genetics Service (RMGS) at St Mary’s Hospital, Manchester, is using a Freedom EVO® liquid handling workstation in combination with chemagen’s proven nucleic isolation technology to perform fully automated DNA extraction. Supplied by chemagen’s UK distributor, Auto Q Biosciences, this integrated system is housed within a Class 1 biological [more...]
Date: 2010-09-02 16:25:00
DNA sequencing reactions all use a primer to initiate DNA synthesis. A Quicktime animation of how dideoxy nucleotides are incorporated into DNA in sequencing reactions. Nucleic acid sequence This sequence is written as a succession of letters representing a real or hypothetical DNA molecule or Sequences can be read from the biological raw material through DNA . DNA Sequencing – Real Time DNA analysis Information With the latest chellenges in DNA Sequencing, our organization looks to unravel the myths, truths and most essential assets in the deconstruction of genes. DNA Sequencing The [more...]
Date: 2010-07-09 03:20:29
Eppendorf to market Akonni TruTip™ « Frederick County Biotech ...
HAUPPAUGE, N.Y. & FREDERICK, Md.–(BUSINESS WIRE)–Eppendorf North America and Akonni Biosystems today announced they have entered into a joint marketing agreement to promote Akonni TruTip nucleic acid extraction kits configured for use with Eppendorf epMotion automated pipetting systems. Under the agreement, Eppendorf will promote the extraction kits to clinical, clinical research and forensic laboratories in North America – providing users with access to the industry’s most rapid and reliable means to automatically extract PCR-ready DNA and/or RNA from larger volume [more...]
Date: 2010-04-26 16:36:03