WCU installs new DNA sequencing equipment | The Reporter
The studies that forensic science faculty members are planning for Western Carolina University’s new next-generation DNA sequencer could help introduce the technology into crime laboratory casework. Brittania Bintz holds a pico-titer plate containing DNA and reagents for sequencing. Western Carolina University forensic science faculty recently completed installation of a new next-generation DNA sequencer called a GS Junior System. WCU acquired and recently completed installation of a GS Junior System, an instrument that Roche Diagnostic Corp. launched globally in May. The instrument [more...]
Date: 2010-09-14 21:02:20
Can I use sheared genomic DNA as starting material of bacterial ...
My question is whether initially sheared genomic dna can be safely used for genome sequencing and assembly by 454 technology. By 'initially', I mean genomic DNA was sheared during extraction process. I attach gel image of my genomic dna samples (marker is lambda dna digested with EcoRI and HindIII). Although I know that dna fragmentation step is involved in standard genome sequencing by GS FLX titanium, I am concerned because I think it might be possible that shearing during extraction can affect randomness of standard fragmentation step and so on. Thanks in [more...]
Date: 2010-10-12 08:03:29
inqaba biotec and BODE Technology to host 2nd Annual African DNA ...
Oligonucleotide synthesis; Sanger sequencing service using the ABI 3130XL and ABI 3500XL sequencers; High-throughput DNA sequencing service using a Roche /454 GS FLX sequencer; Customised services like amplicon cloning, [more...]
Date: 2010-03-01 07:59:59
The Evilutionary Biologist: This Week's Citation Classic
Definitely, the Sanger method started a new epoch. I'm wondering if now we are living the end of the Sanger era. The DNA sequencing technology from 454 Life Sciences seems to be expanding quite fast. If the technology is as good as they say, it might bury the Sanger method into ancient history. Sunday, May 13, 2007 7:11:00 AM [more...]
Date: 2007-05-11 02:56:00
DNA sequencing - the 454 method
Watch the Wellcome Trust's animation about 454 machines, which are able to read one gigabase of DNA sequence in a couple of days, at a cost of $0.02 per 1000 [more...]
Date: 2010-05-21 13:28:22
Researchers question whether bad knees are in the genes
454 DNA sequencing at engencore.sc.edu This entry was written by EnGenCore, posted on September 29, 2010 at 1:32 pm, filed under Uncategorized. Bookmark the permalink. Follow any comments here with the RSS feed for this post. Comments are closed, but you can leave a trackback: Trackback [more...]
Date: 2010-09-29 13:32:45
Genome-Wide DNA Methylation Maps in Follicular Lymphoma Cells ...
Methodology/Principal Findings We performed methylation-enriched genome-wide bisulfite sequencing of FL cells and normal CD19+ B-cells using 454 sequencing technology. The methylated DNA fragments were enriched with methyl-binding proteins, treated with bisulfite, and sequenced using the Roche-454 GS FLX sequencer. The total number of bases covered in the human genome was 18.2 and 49.3 million including 726,003 and 1.3 million CpGs in FL and CD19+ B-cells, respectively. 11,971 and 7,882 methylated regions of interest (MRIs) were identified respectively. The genome-wide distribution of these [more...]
Date: 2010-09-30 21:12:52
Life Technologies To Buy DNA Sequencing Company, Ion Torrent ...
Formed by life sciences pioneer Dr. Jonathan Rothberg, founder of CuraGen, 454 Life Sciences and co-founder of Raindance Technologies, Ion Torrent has revolutionized DNA sequencing by enabling a direct connection between chemical and [more...]
Date: 2010-08-18 12:00:00
Technical Writer – Bioinformatics
CT-Branford, About Roche 454 Life Sciences: Roche 454 Life Sciences develops and commercializes instrumentation, reagents, and services for high-throughput DNA sequencing. Simple, unbiased sample preparation and massively parallel [more...]
Date: 2010-10-10 16:01:57
Roche and IBM Research target whole genome sequencing for $100 to ...
Quick and reliable DNA sequencing could help Roche find patients that react best to specific drugs, helping reduce side-effects and drug development costs. The deal should accelerate Roche's understanding of diseases at a molecular level. One hopes that this can provide the insights that help identify drug targets, pathways and accelerate the development of healthcare solutions for the world's unmet medical needs. Check out more on this youtube video link Schematics of the DNA transistor operation for the control of the translocation of a DNA through a nanopor A membrane containing the [more...]
Date: 2010-07-02 03:13:00
PRESS RELEASE Roche and IBM Collaborate to Develop NanoporeBased ...
PRESS RELEASE: Roche and IBM Collaborate to Develop Nanopore-Based DNA Sequencing Technology Thu Jul 01 00:01:06 2010 EDT Collaboration aims to accelerate human genome analysis and enable advancements in personalized healthcare YORKTOWN [more...]
Date: 2010-07-01 04:02:50
Bioinformatics programmer for Next-Gen DNA Sequencing Core, NYU ...
Location : NYU Langone Medical Center, New York, New York, USA Deadline for applications : August 1, 2010. Description : BACKGROUND: NYU Medical Center operates a core DNA sequencing lab with Illumina and 454 sequencers. The Sequencing Bioinformatics group provides data management with both basic and advanced bioinformatics data analysis. (The search for this position has been re-opened as of July 10, 2010). RESPONSIBILITIES: Manage all aspects of bioinformatics associated with running a 454 Genome Sequencer. Meet with scientists to determine experimental design, set up runs, process [more...]
Date: 2010-07-19 15:44:06
Genomics: The next generation | Wellcome Trust
The advent of the Human Genome Project and the development of faster, cheaper DNA sequencing revolutionised medical and biological research - and the technology is still evolving. Mun-Keat Looi looks at the platforms pushing us closer toward the ‘$1000 genome’. At the turn of the millennium, genomics captured the public's imagination with the publication of the first draft of the human genome and the insights it offered into our biology. When the Human Genome Project started in 1991, DNA sequencing entailed laborious radiation-based methods, with researchers manually loading [more...]
Date: 2009-07-28 11:00:00
Next Generation Sequencing » Tweaking the 454 sequencing protocol ...
In the new issue of Biotechniques, a paper by Maricic and Pääbo (login may be required) describes how a change to the standard 454 sequencing protocol can dramatically increase the size of the library of DNA that goes into the actual sequencing reaction. The trick used is to replace the last step in the library preparation where single stranded DNA is released from streptavidin beads. The original 454 protocol employes NaOH denaturation for this step, but the researchers found that this procedure results in a loss of over 99% of the DNA. However, When they replaced the NaOH denaturation [more...]
Date: 2009-01-07 20:04:53
IDT enters exclusive agreement with 454 Life Sciences to provide ...
IDT enters exclusive agreement with 454 Life Sciences to provide custom primers for GS FLX Titanium Chemistry 4 October 2010 – HPLC purified and MS certified primers for genome sequencing CORALVILLE, IA: Integrated DNA Technologies (IDT), the custom biology company, has signed an exclusive agreement with 454 Life Sciences, a Roche company, to provide researchers with custom primers for the GS FLX Titanium Chemistry, used on the company’s GS FLX System and GS Junior System. Available as FusionPrimers or Rapid Library MID (Molecular Identification) Adaptor Oligos, these products are used [more...]
Date: 2010-10-04 16:16:19
PhD Scholarship for Molecular Ecology and Ecological Epigenetics ...
A (partial) 454 based transcriptome sequence is available, and more than 10.000 SNPs, hundreds of EST-SSRs, and routine protocols for DNA methylation study are available for this species. Recently, an ecological epigenetics line has been started up, which aims to investigate in what way DNA methylation could be important for rapid adaptation to rapidly changing environmental conditions. A central theme in all lines is how inbreeding affects gene expression, and how inbreeding might disrupt epigenetic regulation mechanisms. The institute is well equipped, has a very modern molecular lab, and [more...]
Date: 2010-10-12 12:30:00
PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading
Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]
Date: 2009-11-13 08:00:00
Comments: Life Technologies buys Ion Torrent « Omically Speaking
There was some exciting news yesterday from the next-generation sequencing (NGS) field, with the buy-out of Ion Torrent - a start-up that’s developed a novel DNA sequencing machine – by the life-sciences behemoth Life Technologies (for a whopping 350million dollars). The technology for sequencing of DNA – the blueprint of all life – is at an exciting stage. Ten years ago, the complete human genome was sequenced at the cost of several billion dollars and decades of attempt. Today, a full genome can be sequenced for as little as $10,000 and in less than a week. The price and time are [more...]
Date: 2010-08-18 17:13:21
EpiCentral: Examining blocking lesions in ancient DNA
Blocking lesions prevent amplification and sequencing of affected molecules, thus limiting the analysis of DNA derived from ancient samples. Heyn et al. recently developed a new method--polymerase extension profiling (PEP)--that reveals occurrences of polymerase stalling on DNA templates. This sequencing-based technology allows detection of damage on a single-molecule level. The technique used CircLigase™ ssDNA Ligase for high-efficiency ligation of single-stranded adaptors (containing the Roche 454 A sequence) to the 3’ ends of primer-extension products. The authors found evidence of [more...]
Date: 2010-10-07 20:29:00
Blogging the Singularity » DNA Sequencing: New directions, and ...
DNA sequencing technology has continued to advance since the Human Genome Project, a task that took over a decade, hundreds of person years, at a cost of $3 billion. Subsequent technology developments, fueled by innovations in nucleic acid chemistry and detection have provided about a million-fold reduction in cost and equally dramatic improvement in throughput. However, much lower cost and more efficient methods for DNA sequencing will be required to make it feasible for routine healthcare practice and to reach the vision of personalized medicine. A team at IBM Research, in collaboration [more...]
Date: 2010-10-11 15:04:24