Our blogs and videos choice: dna

DNA: The Tree of Life ( Humanity Hacking Immortality )

Visit my website http Seek Knowledge for “Life” Music by korn (Evolution) Visit my blog key-universe.blogspot.com DNA en.wikipedia.org Nucleic acid en.wikipedia.org Genetics en.wikipedia.org Protein en.wikipedia.org RNA en.wikipedia.org Polymer en.wikipedia.org Nucleotide en.wikipedia.org Nucleobase en.wikipedia.org Genetic code en.wikipedia.org Amino acid en.wikipedia.org Chromosome en.wikipedia.org DNA replication en.wikipedia.org Eukaryote en.wikipedia.org Helix en.wikipedia.org DNA sequencing en.wikipedia.org Junk DNA en.wikipedia.org Triple-stranded DNA en.wikipedia.org History of [more...]

Date: 2010-08-27 22:27:16

Blog posts (151) | Videos (31)

mrna dna | DNA Sequencing

Translation: DNA to mRNA to Protein | Learn Science at Scitable Genes encode proteins, and the instructions for making proteins are decoded in two steps: first, a messenger RNA (mRNA) molecule is produced through the transcription . Mrna Dna | TutorVista | Missense mRNA molecules are created when template DNA strands or the mRNA strands themselves undergo a It is synthesised from DNA as and when necessary. It carries. Complementary DNA The central dogma of molecular biology outlines that in synthesizing proteins, DNA is transcribed into mRNA, which is translated into protein. Possibly [more...]

Date: 2010-06-30 09:52:38

DNA Polymerase and other factors

DNA polymerase can add free nucleotides to only the 3’ end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, therefore, needs a primer at which it can add the first nucleotide. Primers consist of RNA and DNA bases with the first two bases always being RNA, and are synthesized by another enzyme called primase. An enzyme known as a helicase is required to unwind DNA from a double-strand structure to a [more...]

Date: 2008-07-27 11:03:00

Scientist Solutions - DNA MicroArray Basics

Just came across this resource today, while looking up MicroArray companies. Its pretty nice and a great beginner step. DNA microarrays can be thought of as the massively parallel version of Northern blotting. While the Northern blotting technique is capable of analysing for each experiment the expression of one single gene in different conditions, microarrays allow the exploration of the expression levels of thousands of genes in several different conditions in one single run. As Northern blotting and many other classic techniques widely used in molecular biology, the fundamental basis of [more...]

Date: 2009-03-19 19:31:20

Berkeley Lab News Center Press Release: New Electrostatic-based ...

In a typical experiment, a microarray is prepared and mounted in a well chamber and the DNA is hybridized (a standard technique in which complementary single strands of DNA bind to form double-stranded DNA "hybrids"). A suspension of negatively-charged silica microspheres is then dispersed through gravitational sedimentation over the microarray surface, a process which takes about 20 minutes. Because the substrate or background surface of the microassay is positively charged, the silica microspheres will spread across the entire surface and adhere to it. However, on surface areas containing [more...]

Date: 2008-06-30 16:00:00

Caffeine King: DNA Lemon Lime Energy Drink Review

DNA Lemon Lime comes in a mostly black can, with a yellow skull in a green star. Surprisingly, it has a lack of emphasis on the DNA strand behind the bare cranium. You'd expect with the name "DNA," that the can would have a scientific inspired theme, instead of the No Fear-type look used. Either way, the design is still simple without being empty, and is aggressive without overemphasizing it. A light, almost grainy lime begins the flavour, with its sweetness being more defiant and brazen than its sourness, resulting in it having a confection-like quality. Then there's a quiet birth of lemon [more...]

Date: 2010-06-04 19:06:00

Could taking a nutritional supplement keep you young?

It might be more real than not. Scientists working at the biotech giant Geron Corporation have isolated a molecule, TA-65, which has the ability to increase telomerase activity (It is important to note that his find has not been evaluated in published peer reviewed studies as of yet). This is an exciting breakthrough for telomerase is an enzyme that adds DNA sequence repeats to the 3′ end of DNA strands in the telomere regions, which are found at the ends of eukaryotic chromosomes. The telomeres contain condensed DNA material, giving stability to the chromosomes. These telomere DNA [more...]

Date: 2010-07-14 19:14:34

A step forward in understanding the BRCA2 'cancer gene' « Cancer ...

“Understanding how BRCA2 masterminds the DNA repair kit to prevent tumour formation is a vital step towards developing new treatments for this type of breast cancer.” This discovery is not going to immediately change the way that people with BRCA2-related cancer are treated. But it’s an extremely important finding for cancer and DNA repair researchers around the world, as they work to understand the disease and develop more effective treatments. As we’ve written about before, half the battle in cancer research is developing the tools and techniques to answer vital questions. And now [more...]

Date: 2010-08-27 09:49:03

Expression Profiling of PBMC-based Diagnostic Gene Markers ...

Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients Shigeto Kobayashi1, Akihiko Ito2, Daisuke Okuzaki3,4, Hiroaki Onda3,5, Norikazu Yabuta3, Ippei Nagamori3, Kazuo Suzuki6,7, Hiroshi Hashimoto1 and Hiroshi Nojima3,4,5,* 1Department of Rheumatology and Internal Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan 2Division of Molecular Pathology, Department of Cancer Biology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan 3Department of [more...]

Date: 2010-09-04 18:43:52

Sandwalk: Nobel Laureate: Walter Gilbert

The Nobel Prize in Chemistry 1980. "for their contributions concerning the determination of base sequences in nucleic acids" Walter Gilbert (1932 - ) was awarded the Nobel Prize for developing a chemical method of sequencing DNA (with Allan Maxam). The method relied on chemical reactions that cleaved DNA at specific residues. By carrying out partial reactions where only one cleavage occurred in each DNA strand, it was possible to separate the cleavage products on an acrylamide gel and determine the position of each residue by the length of the fragment. The chemical sequencing strategy has [more...]

Date: 2008-12-10 14:35:00

[DNA: Replication, Repair, and Recombination] Inefficient ...

Inefficient Proofreading and Biased Error Rates during Inaccurate DNA Synthesis by a Mutant Derivative of Saccharomyces cerevisiae DNA Polymerase δ* Stephanie A. Nick McElhinny ‡ , Carrie M. Stith § , Peter M. J. Burgers § and Thomas A. Kunkel ‡ ‡Laboratory of Molecular Genetics and Laboratory of Structural Biology, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709 and the §Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110 1 To whom [more...]

Date: 2007-01-19 08:00:00

Artificial Life Created in a Laboratory for the First Time ...

They manufactured a new chromosome from artificial DNA in a test tube, then transferred it into an empty cell and watched it multiply – the very definition of being alive. The man-made single cell “creature”, which is a modified version of one of the simplest bacteria on earth, proves that the technology works. Now Dr Venter believes organism, nicknamed Synthia, will pave the way for more complex creatures that can transform environmental waste into clean fuel, vaccinate against disease and soak up pollution. But his development has also triggered debate over the ethics of “playing [more...]

Date: 2010-05-21 13:46:05

autism center of pittsburgh – Do Cell Phones Really Cause Cancer ...

Drs. Singh and Lai reported a decade ago that cell phone radiation quickly causes DNA single-strand and double-strand breaks at well below the current federal safety level for exposure. This causes a condition called micronuclei. Dr. George Carlo is a public health expert who conducted wireless technology research. He showed that human blood exposed to cell phone radiation had a 300% increase in genetic damage in the form of micronuclei. He says that wireless technology is “potentially the biggest health insult” this nation has ever seen, bigger than cigarette smoking and asbestos. Even [more...]

Date: 2010-10-13 13:00:17

$1.00 - Bio2010

DNA polymerase I catalyzes the elongation of the new DNA strands in prokaryotes. c. The lagging strand is synthesized as Okazaki fragments. d. The leading strand elongates from its 3' to 5' end. e. Helicases initiate replication by winding the two strands together. Save Answer 16. (Points: 5) The appropriate adjective to describe DNA replication is a. progressive. b. lytic. c. semiconservative. d. nondisruptive. e. natural. Save Answer 17. (Points: 5) In a sample of double-stranded DNA from a human, you have determined that 20 percent of the nitrogenous base is adenine. What percentage should [more...]

Date: 2008-08-05 01:50:23

E. Coli Plasmid Vectors: Methods and Applications (Methods in ...

E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) (archive) E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, N.J.), V. 235.) Table of Contents The Function and Organization of Plasmids Finbarr Hayes Choosing a Cloning Vector Andrew Preston Escherichia coli Host Strains Nicola Casali Chemical Transformation of E. coli W. Edward Swords Electroporation of E. coli Claire A. Woodall DNA Transfer by Bacterial Conjugation [more...]

Date: 2004-04-04 08:00:00

A non-radioactive, PFGE-based assay for low levels of DNA double ...

A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells This digital document is a journal article from DNA Repair, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: A PFGE method was adapted to measure DNA double-strand breaks (DSBs) in mammalian [more...]

Date: 2010-09-10 23:55:39

The Randomly Grad Life: My Research Explained: Polymerase Chain ...

New primers attach to your melted DNA. One primer binds to old template DNA and another primers binds to the newly extended DNA. The polymerase once again extends on each strand. On the template strand, the same thing as above happens. But on the new strand, the polymerase will stop because the DNA doesn't go forever. It ends exactly at the end of the first primer. Then the temp raises to 95C and we are ready for the next cycle. Now we almost have exactly what we need. After a few cycles the amount of DNA with ends is in a much larger excess than template DNA and just long copies and that [more...]

Date: 2010-04-21 16:00:00

What characteristics of DNA polymerase III make it an ideal enzyme ...

The key features of DNA polymerase III are its catalytic potency, its fidelity, and its processivity. It is able to catalyze the addition of ~103 bases per second, compared to only about 10 per second for DNA polymerase I,with an error frequency of about 1:104–105. One of the unique features of DNA polymerase III is its ability to continuously synthesize very long (thousands of bases) stretches of DNA, unlike DNA polymerase I. This is a consequence of its processivity; the β2 sliding clamps ensure that it remains bound to the template strand DNA. In contrast, DNA polymerase I, without a [more...]

Date: 2010-03-07 11:59:00

What Does DNA Stand For in Science | Intelligent Mag

Before cells are divided, the chromosomes duplicate first; this is what we call replication of DNA. This is the division of cells which results in two cells that have to contain the same information like their mother-cell. There are four nitrogenous bases within the DNA molecule. Those are: Adenine, Cytosine, Thymine and Guanine. Genes are composed of bases. In case of DNA damaging, the sequence of DNA will be changed. This happens when there is mutagen factor involved. These mutagens are highly radioactive. The damage that occurs as a result of the radiation is determined by the mutagen [more...]

Date: 2010-10-09 19:47:49

Dr. Pandey “pulls off” new DNA sequencing method

The current method to sequence DNA is called gel-electrophoresis. This process is very costly and time consuming. However, Dr. Pandey and his research team have proposed a way to make DNA sequencing far easier and cheaper than the current method. For those of you scratching your heads and wondering what the purpose of DNA sequencing could possibly be, imagine in the futurew going to the doctor and getting customized medicine just for you based on your DNA. This medicine would do exactly what it needed to in order to fix your problem because it was made specifically for you and only you. [more...]

Date: 2010-09-23 17:36:11

physics nobel prize awared to andre geim and konstantin novoselov ...

Top news :  Physics Nobel goes to graphene researchers DNA Interacts with Graphene An illustration of how fluorescent-tagged DNA interacts with functionalized graphene. Both single-stranded DNA (A) and double-stranded DNA (B) are adsorbed onto a graphene surface, but the interaction is stronger with ssDNA, causing the fluorescence on the ssDNA to darken more. C) A complimentary DNA nears the ssDNA and causes the adsorbed ssDNA to detach from the graphene surface. Original post: Grephene on Twitter Top news articlesGrephene, grephene nobel, Grephene on [more...]

Date: 2010-10-05 17:39:35

Antibody Therapies and the New Generation of DNA Sequencing ...

Our antibody database targets protein-coding genes. Although they form only 1% of the total human genome, they account for 85% of the mutations that lead to disease. DNA sequencing (defining the sequence of the 4 base amino acids within the DNA strand) and then using antibodies specific to those sequences, is a vital part of disease research. Sanger sequencing is the standard for doing this. However, in 2007 a next generation (or next gen) sequencing technique was devised. Lauded as the biggest advancement in genomics since microarray technology, it has radically improved diagnostic [more...]

Date: 2010-09-27 10:34:46

DNA strides into organic synthesis

The walker and three 'stations', made from specific DNA sequences, organise themselves along the track using the normal pairing of DNA bases. 'The walker will move unidirectionally along the track,' Liu says. 'We attached chemical reactants to the walker and each station on the track. As the DNA walker moves from station to station, a programmed series of chemical reactions takes place.' The walking strand of DNA moves to each station (blue, red and green) in turn and reacts with the attached reagent © Nature Nanotechnol. In this way, the Harvard team was able to perform three acylation [more...]

Date: 2010-10-10 22:46:00

Molecular Biology Lecture III Prof. Graham Walker Video | DnaTube ...

This is what's known as the leading strand because DNA, the synthesis of the new strand can go -- Which is going 5 prime to 3 prime is going in the same direction as the movement of the replication fork. ... nAnd it's continually jumping. And I told you that the little RNA primer is used to start each strand. And then the DNA polymerase is able to elongate that. And then at the end these little nicks in here, the RNA has to be removed, fill in the gap and then it's sealed [more...]

Date: 2009-02-04 08:00:00

Nick Denton: Zuckerberg's Original Idea for Facebook Was Dark ...

Facebook CEO Mаrk Zuckerberg іѕ mentioned 10 times in Ben McGrath’s much ballyhooed Nеw Yorker profile οn blog impressario Nick Denton. Thе mοѕt interesting mention іѕ in thе context οf a story аbουt Zuckerberg and Denton serving οn a panel together at a News Corp. retreat in Monterey, a fact whісh in and οf itself іѕ kind οf ominous: “I іn fact like thе guy,” he said. “Apparently, his first thουght for Facebook wаѕ this dаrk Facebook. like, thе thουght wаѕ that іt wаѕ going to bе a place for people to bitch аbουt each other, and then іt [more...]

Date: 2010-10-13 12:01:14

the Foresight Institute » DNA springs enable mechanical control of ...

Further, structural DNA nanotechnology, particularly scaffolded DNA origami, has been proposed to provide frameworks for use in nanotechnology to spatially organize functional components (see, for example “Modular DNA nanotubes provide programmable scaffolds for nanotechnology” and “Advancing nanotechnology by organizing functional components on addressable DNA scaffolds“. Now, these two threads, mechanical control of chemical reactions and DNA nanotechnology have been brought together. Stuart Wolpert writes from the UCLA newsroom “UCLA physicists control chemical reactions [more...]

Date: 2010-10-08 23:45:02

BMI1-mediated histone ubiquitylation promotes DNA double-strand ...

We found that BMI1 and RING2 are recruited to sites of DNA double-strand breaks (DSBs) where they contribute to the ubiquitylation of γ-H2AX. In the absence of BMI1, several proteins dependent on ubiquitin signaling, including 53BP1, BRCA1, and RAP80, are impaired in recruitment to DSBs. Loss of BMI1 sensitizes cells to ionizing radiation to the same extent as loss of RNF8. The simultaneous depletion of both proteins revealed an additive increase in radiation sensitivity. These data uncover an unexpected link between the polycomb and the DNA damage response pathways, and suggest a novel [more...]

Date: 2010-10-06 20:38:28

Starchild Skull 2010 DNA Result - Earth Changes and the Pole Shift

Part of the nuclear DNA have can thus reduced to its ( expected ) of human origin, while have surrendered to others subsections at all , no significant similarities and similarities to other earthly creatures. That is, the genotype of the father was definitely not found in the database of the National Institutes of Health (NIH ) in Maryland. The NIH is the world 's largest database of genetic information of many species of viruses , bacteria , crustaceans, fish to all types of plants and animals , including primates and humans. These results of DNA analysis were repeated several times and [more...]

Date: 2010-09-26 08:31:32

Plant DNA Recombinases: A Long Way to Go

Mutants sensitive to DNA damaging agents like UV and ionizing radiation showed deficiency in recombination . The complex nature of the process and crosstalk between homologous recombination and other pathways like transcription, repair , ... At this stage, the newly formed double stranded DNA has the old parental strand and a new incoming strand. It is therefore called heteroduplex. These heteroduplexes further extended to mediate complete strand exchange . [more...]

Date: 2009-12-14 03:07:53

Florida State: A-maize-ing DNA research – Vital Signs Health Blog ...

Florida State: A-maize-ing DNA research Uncategorized — posted by Marissa Cevallos on September, 14 2010 2:57 PM Two ears of buttered corn lie on a plate. Inspect them for just a few seconds, and you’d find some telltale bump or abnormality to tell them apart. If they came from the same kind of seed with the same genetic material, why don’t they look exactly the same? Scientists still don’t know why cells with the same genome can have different traits. But that’s the puzzle that Florida State University and Florida A&M University biologists are looking to solve with a $1.4 million [more...]

Date: 2010-09-14 18:57:41

Vector Art | Free Clip Art | Cancer Icons, Ribbon, Double Helix ...

The vector package contains 4 eps illustrator vectors of cancer icons, which include: no smoking sign, cancer ribbon, dna strand, and nucleus graphic. These vectors are usable to anyone running adobe illustrator 8 or higher. I would encourage all you good hearted designers to donate or volunteer online or at a local cancer related care center or hospital- to show the world that designers do care. For more great graphic design vector resources please check out our sponsor’s ads above. Enjoy. Click here to download Cancer Vectors Free 10 Responses to “Free Illustrator Vectors- April is [more...]

Date: 2008-04-10 04:05:17

Gel electrophoresis separates DNA fragments based on their…?

Question by Nicole Has-Ellison: Gel electrophoresis separates DNA fragments based on their…? A) recognition sequence B) length C) positive charge D) chemical bonds Best answer: Answer by BP B) length but others things like salt, whether the DNA is linear or a circle, whether the DNA is single or double stranded, whether the DNA is intact or has nicks in one or both of it’s strands all affect its speed of migration in a gel… What do you think? Answer [more...]

Date: 2010-10-09 04:10:59

ThinkGeek :: DNA Portrait

It's art! It's you! Your deoxyribonucleic acid is what makes you the unique snowflake you are. The genetic dance of Adenine, Thymine, Guanine and Cytosine, in infinite combinations, has its own beauty. With the advent of forensic electrophoresis, your own beautiful genetic makeup can be your own personalized art. First, we collect a few cheek cells. It's a painless process - just swipe the inside of your mouth with a cotton swab. Then, you send your sample to our labs where our monkey scientists replicate a few clone-troopers extra strands of DNA, dye them, embed them in a gel, and apply [more...]

Date: 2009-06-11 20:41:14

Feline Fur Flushes Out Felons

The hair cats leave in their owners' homes through their frequent grooming can help authorities to catch criminals. Here, Catster member Leo shows off his grooming technique. New research has revealed that cat fur can help to identify and convict criminals. Dr. Leslie Lyons, a cat genetics expert, pioneered the research. Lyons, who works at the University of California Davis School of Veterinary Medicine, was already compiling information on cat DNA, when she had an “A-ha!” moment. “I’m a big fan of CSI: Crime Scene Investigation,” she says. In two episodes of [more...]

Date: 2010-09-29 14:43:50

Select double-stranded DNA binding sites for transcription factors.

Can double-stranded DNA 'decoys' of transcription factors be used as drugs? How might this work? Has anyone done this?How could your data be used to find otherwise unknown transcription factor binding sites in a genome?How could your data be used to map transcriptional networks in a newly sequenced genome?How could you get data on multiple transcription factors simultaneously or in [more...]

Date: 2010-08-20 03:21:00


The accuracy of DNA fingerprinting has been challenged for several reasons. First, because DNA segments rather than complete DNA strands are “fingerprinted”; a DNA fingerprint may not be unique; large-scale research to confirm the [more...]

Date: 2010-09-17 06:44:46

Error-Prone DNA Repair Activity during Somatic Hypermutation in ...

Duplications and deletions were associated with N additions, suggesting participation of a DNA polymerase with some degree of template independence during the repair of DNA breaks initiated by activation-induced cytidine deaminase. Other mutations were consistent with some in vitro activities of mammalian translesion DNA polymerase η: tandem base substitutions, strand slippage, and small insertions/deletions. The nature of substitution patterns shows that DNA lesions at shark Ig genes recruit DNA repair factors with a species-specific repertoire of activities. We speculate that the tandem [more...]

Date: 2010-10-07 06:53:59

DNA sequencing » Blog Archive » DNA Sequencing Methods

DNA Sequencing – How DNA Sequencing Works – Methods for … An explanation of how DNA sequencing works. … Typically, the automated sequencing method is only accurate for sequences up to a maximum of about 700-800 base-pairs in length. … DNA Sequencing Simulation (Sanger Method) Within each reaction mixture are the following items: a DNA segment of unknown … The other DNA sequencing method (named for Allan Maxam and Walter … Next-Generation DNA Sequencing Methods 921k – Adobe PDF – View as html generation DNA sequencing technologies highlight the striking impact … [more...]

Date: 2010-09-28 04:29:58

A quick 5 second animation of the chemistry involved in DNA synthesis

Click here for the short animation: http://bioweb.uwlax.edu/GenWeb/Molecular/Theory/DNA_synthesis/dnasynth.mov And here's the explanation: When DNA is synthesized the free 3´ hydroxyl (OH) group from the growing strand of DNA attacks the a phosphate on the next base to be added (see the red arrows below). Pyrophosphate is released and the new base forms a phosphodiester bond with the growing strand of DNA. The free 3´ hydroxyl group is then free to attack the next base to be added. This reaction is catalyzed by DNA polymerases. Similar reactions are catalyzed by RNA polymerases in the [more...]

Date: 2009-02-19 07:13:00

TopBP1 and DNA polymerase-α directly recruit the 9-1-1 complex to ...

TopBP1 and DNA polymerase-α directly recruit the 9-1-1 complex to stalled DNA replication forks Shan Yan and W. Matthew Michael The Biological Laboratories, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138 Correspondence to W. Matthew Michael: mmichael{at}fas.harvard.edu Abstract TopBP1 and the Rad9–Rad1–Hus1 (9-1-1) complex activate the ataxia telangiectasia mutated and Rad3-related (ATR) protein kinase at stalled replication forks. ATR is recruited to stalled forks through its binding partner, ATR-interacting protein (ATRIP); however, it is unclear [more...]

Date: 2009-03-23 07:00:00

Yearning for (re)animate Nature: Faunal Colonialism in ...

In the case of woolly mammoths found frozen in permafrost—which are among the best preserved specimens of extinct Pleistocene fauna—only short strands of mitochondrial DNA have been recovered—not the nuclear DNA that would be necessary for cloning (Lindahl 1999)”. Cited in Yule. (Wayne et al. also confirm the 100,000 years limit.) But in the future, as they say, it could be possible. And as an aside, We wonder when Bog’men’ Icemen, peat men etc and mummies, will be cloned, and the possible themepark/spectacular forms this may [more...]

Date: 2010-10-09 10:39:04

DNA Isolation and Modeling « NWABR

This entertaining and inexpensive protocol was adapted from Biotechnology in the Classroom, UC Davis and Diane Sweeney, Biology — Exploring Life. First we, the teacher-students line up for our materials: Dr. Hutchison regales us as we select strawberries and collect materials: This flexible protocol has been used with first graders all the way through high school and administrators. You can make it as in depth as you like. All materials can be bought in a grocery store, there are no expensive reagents. This was my lovely strawberry, in a sealed plastic bag and on a paper towel, [more...]

Date: 2010-03-07 00:00:57

Strand Displacement by DNA Polymerase III Occurs through a τ-ψ-χ ...

In addition to the well characterized processive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA templates, the enzyme possesses an intrinsic strand displacement activity on flapped templates. [more...]

Date: 2009-11-06 14:36:37

DNA: Size Does Matter. But is it Science or Art? « Exploratorium ...

Then I randomly found this website, which starts with the same idea as our DNA Cheek Cell Extraction Demo but takes it to a whole new level:  You send your cheek cells in the mail to their company.  They extract your DNA from your cells, then use PCR to amplify unique sections of DNA in your genome.  This DNA is loaded into a gel and a current is run through the gel to separate the DNA based on size.  UV dye is added to the whole thing to make your DNA strands visible, a photograph is taken, and voila!…a giant canvas print with the color scheme of your choice is ready to hang on your [more...]

Date: 2008-05-14 07:37:47

DNA Fingerprinting : I INTRODUCTION DNA Fing...

Accuracy of Results The accuracy of DNA fingerprinting has been challenged. First, because DNA segments rather than complete DNA strands are “fingerprinted”, there is a possibility that DNA samples taken from two individuals may yield [more...]

Date: 2005-08-12 23:36:00

Are Cell Phones and WiFi Hazardous to Your Health | All Global ...

9. increase the permeability of the blood-brain barrier; H. Lai, NP Singh, “Mangetic Field Induced DNA Strand Breaks in Brain Cells of the Rat,” Env Health Perspect, 5/04, pp. 687-94; H. Lai, “Evidence for Genotoxic Effects (RFR and ELF Genotoxity,” BioInitiative Report, 7/07; Levitt 10. Cause brain cells to leak: A. Goldsworthy, “Electromagnetic Fields and Health: Exec Report,” EM Radiation Research Trust, 2009. 11. H. Lai, NP Singh, “Mangetic Field Induced DNA Strand Breaks in Brain Cells of the Rat,” Env Health Perspect, 5/04, pp. 687-94; Levitt 12. Biological Effects of [more...]

Date: 2010-10-13 13:22:10

Next Generation Sequencing » Tweaking the 454 sequencing protocol ...

In the new issue of Biotechniques, a paper by Maricic and Pääbo (login may be required) describes how a change to the standard 454 sequencing protocol  can dramatically increase the size of the library of DNA that goes into the actual sequencing reaction. The trick used is to replace the last step in the library preparation where single stranded DNA is released from streptavidin beads. The original 454 protocol employes NaOH denaturation for this step, but the researchers found that this procedure results in a loss of over 99% of the DNA. However, When they replaced the NaOH denaturation [more...]

Date: 2009-01-07 20:04:53

DNA and the 'magic rings' trick | UC Health

A new study from UC Davis shows how, like a conjuring trick with interlocking rings, two interlocked pieces of DNA are separated after DNA is copied or repaired. The finding was published online Oct. 10 in the journal Nature Structural and Molecular Biology.While reconstituting the DNA repair system of yeast in a test tube, the researchers found that a complex of proteins called Sgs1, Top3 and Rmi1 allow one DNA strand to open and the other to pass through. “This protein complex does what magicians do,” said lead researcher Stephen Kowalczykowski, distinguished professor of microbiology [more...]

Date: 2010-10-12 17:23:14

DNA Sequencing Videos,Photos,Informations,Details,Collections ...

DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA. The most popular method for doing this is called the dideoxy method or Sanger method (named after its inventor, Frederick Sanger, who was awarded the 1980 Nobel prize in chemistry for this achievment). DNA is synthesized from four deoxynucleotide triphosphates. The top formula shows one of them: deoxythymidine triphosphate (dTTP). Each new nucleotide is added to the 3′ -OH group of the last nucleotide added. Link to discussion of DNA synthesis. The dideoxy method gets its name from [more...]

Date: 2010-08-25 06:59:00

DNA replication

The leading strand is the template strand of the DNA double helix so that the replication fork moves along it in the 3' to 5' direction. This allows the new strand synthesized complementary to it to be synthesized 5' to 3' in the same [more...]

Date: 2010-06-28 03:08:46

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