Searching for cellular switches
As he “engaged science further” as an undergraduate at the University of California, San Diego, he began to appreciate how those basic atoms and molecules came together to create the building blocks of life, such as DNA, RNA and the proteins that control the body."Understanding these basic components of life made me want to further explore how biology, chemistry and physics met at a molecular interface within cells," Buchler said. He first chose to focus on physics then directed his studies more toward biology and theory as a doctoral student at the University of Michigan in Ann [more...]
Date: 2010-10-12 04:00:00
The cell nucleus – a mystery of evolution | DNA Sequencing
For example, eachDrosophila nucleus contains four pairs of chromosomes, each containing 23 pairs of human core. Chromosomes consist of DNA-histone proteins as a wound coil wire wound. If this DNA is wrapped around the genes of genes, those copied into RNA, which contain DNA. The soul itself is a double membrane separating the contents enclosed by the nuclear cytoplasm of the cell keeps the rest. This double membrane is studdedlet certain molecules pass through pores. RNA copies of genes, for example, pass through the pores from the nucleus to the cytoplasm e. There are activities for the [more...]
Date: 2010-10-06 14:50:15
Best 2010 Ebook Collection Part 4
Dean J.R. - - - Extraction Methods for Environmental Analysis Dean M. - - - The Human ATP-Binding Cassette (ABC) Transporter Superfamily Dean M.W. - - - $30 Music School Dean T. - Gleek J. - - Internet Marketing Secrets Revealed Deans G.K. - Kroeger F. - Zeisel S. - Winning the Merger Endgame: A Playbook for Profiting from Industry Consolidation Deans P.C. - - - E-commerce and M-commerce technologies Deb S. - - - Video data management and information retrieval Deb S. (ed.) - Deb S.P. (ed.) - - p53 Protocols Debarre O. - - - Higher-Dimensional Algebraic Geometry Debevec P. - - - [more...]
Date: 2010-10-11 18:41:15
Coralville's IDT Partners with Marshall University
The Marshall Institute for Interdisciplinary Research (MIIR) has announced it has entered into an applied research and product development partnership with leading biotechnology company Integrated DNA Technologies (IDT). According to the terms of the agreement, scientists at MIIR will be developing optimized biomolecular analyses or “assays” to be used by IDT for the detection and quantification of ribonucleic acid (RNA). The goal of the co-development project is to significantly improve the specificity of IDT’s current assay methods without substantially increasing the cost. IDT’s [more...]
Date: 2010-05-21 20:59:12
5 minute DNA Extraction in a Shot Glass
A blog that offers research to help you achieve and maintain health… "If we don't play God, who will?" – James Watson Intro: 5 minute DNA Extraction in a Shot Glass Despite its exotic-sounding name, DNA is ubiquitous – it can be found in every cell of every living thing and almost everywhere on the planet. Nonetheless, we rarely come face-to-face with the molecule itself – and it’s not because DNA is difficult to find or isolate! In this instructable, we’ll show you how to isolate your own DNA with little more than some dish soap, table salt, high-proof alcohol, a shot glass, and [more...]
Date: 2009-11-13 04:41:42
Healthcare Sales & Marketing Network: James Godsey, Ph.D., to Join ...
Mr. Jones added that "Dr. Lorincz has led Digene's research since the company's founding. He was responsible for the development of the company's lead product - the Hybrid Capture® 2 HPV DNA Test. By expanding our scientific leadership team, Dr. Lorincz will have more time to foster our relationships with global scientific thought leaders and to explore possible new 'frontiers' for the company's growth." In addition to his work at Veridex and GenProbe, Dr. Godsey was President/COO of ThermoGenesis Corporation and he held a number of increasingly responsible positions with DADE Microscan [more...]
Date: 2006-10-27 21:26:00
JoVE: Electroeluting DNA Fragments (Video Protocol)
Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution from glass or silica particles, DEAE-cellulose membranes, "crush and soak method", electroelution or very often expensive commercial purification kits. Thus, selecting a method will depend mostly of what is available in the laboratory. The [more...]
Date: 2010-09-05 22:00:00
CIENCIASMEDICASNEWS: New Functions For New Genes, Non-coding RNAs ...
DNA regions have been known for many years to confer both negative and positive regulation of protein synthesis. "The fact that RNA is much easier to manipulate than DNA could mean that this discovery has a vast therapeutic potential" explains Ørom. The work has been led by Ramin Shiekhattar, group leader at the Centre for Genomic Regulation (CRG) in Barcelona and now at the Wistar Institute and co-authored by Cedric Notredame and Roderic Guigo from the Bioinformatics and Genomics Programme at the Centre for Genomic Regulation (CRG). Shiekhattar and collaborators have seen a new role for a [more...]
Date: 2010-10-08 13:03:00
Plant RNA/DNA Purification Kit
Total DNA and RNA from 50 mg of tobacco, tomato and peach were simultaneously isolated with Bio-Synthesis's Plant RNA/DNA Purification Kit (with the optional on-column RNase treatment). Next, 2µL of DNA from each 150 µL elution was mixed in 20 µL of PCR reaction and used in a real-time PCR (95ºC for 3min and 40 cycles at 95 ºC for 15 sec. and 60 ºC for 30 sec.). DNA from tobacco (green), tomato (red) and peach (blue) was successfully amplified, indicating the high quality of the DNA for downstream application. Primer dimer (black) also was shown. Figure 3. Isolate High Quality Total RNA [more...]
Date: 2010-08-16 04:44:00
LAB TECH – DUKE – Molecular biology/evolution/genomics (Durham, NC ...
DUTIES: Responsibilities will include: DNA and RNA extractions, sample prep for Illumina library construction and sequencing, standard and quantitative real-time PCR, maintenance of live insects in the lab, as well as lab organization, ordering, and management. Experience with any or all of the above is desirable. The first few weeks of the position will involve helping to set up the lab (ordering, etc). Depending on skills and interests, opportunities could also include genome sequence analysis, use of molecular databases, molecular phylogenetics, design of PCR and sequencing primers, field [more...]
Date: 2010-10-11 18:43:41
New Class of Objects Encoded Within the Genome: Non-Coding RNA ...
The idea that molecules of RNA can have a DNA-regulating effect is well established. More than 1,000 so-called microRNAs are known to science, for example, and their effect on silencing genes has been well described. According to Shiekhattar, he assumed that long ncRNAs would also silence genes, not promote their activation. To his surprise, the researchers found that depleting a cell of ncRNAs actually decreased the degree of overall gene expression of neighboring genes, revealing a role for ncRNAs in potentiating gene expression. In fact, when Shiekhattar and his colleagues depleted adult [more...]
Date: 2010-10-07 01:31:09
ERV's latest blog on the FDA paper. One step too far?.
PNAS paper, using standard PCR to ID 'positives' and 'negatives', with insane numbers of bands as a result of non-specific primer binding. Insane numbers. As if this is 1990 and we dont know what 'Real Time PCR' and 'Taqman Probes' are. Im going to ignore that and grant the premise they really have IDed viral sequences. And they arent XMRV. They dont cluster anywhere near XMRV on a phylogenetic tree. Theyre something else entirely. Okay, well, logically it could be contamination from mouse DNA. So they checked for that... by looking for mouse mitochondrial DNA. *blink* I understand [more...]
Date: 2010-08-23 07:00:00
Science Webulagam: Ribonucleic acid (RNA)
Ribonucleic acid (RNA) is a biologically important type of molecule that consists of a long chain of nucleotide units. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. RNA is very similar to DNA, but differs in a few important structural details: in the cell, RNA is usually single-stranded, while DNA is usually double-stranded; RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom); and RNA has the base uracil rather than thymine that is present in DNA. RNA is transcribed from DNA by enzymes called RNA [more...]
Date: 2010-04-27 13:28:00
FinchTalk: From Reads to Data Sets, Why Next Gen is not like ...
The first widely-used sequencing systems were based on the “Sanger” method. DNA was synthesized in the presence of chain terminating radioactive dideoxy-nucleotides (1). Mixtures of DNA fragments were separated by size using gel electrophoresis and the bases were identified and entered into a computer through manual techniques. Automated DNA sequencing instruments arrived later. These instruments made DNA sequencing thousands of times more efficient by detecting fluorescently labeled fragments and sending the information directly to a computer (2). For the first time, it became possible [more...]
Date: 2009-01-06 19:16:00
Upcoming Next-Gen Sequencing Conferences | RNA-Seq Blog
NeXt-GENeration sequencing platforms create sequence reads of DNA fragments for genome variation studies, RNA for transcriptome studies, DNA-protein interactions for epigenetic studies, and chromosomal DNA of large genome nucleotide variations for copy number studies. The X-Gen Congress and Expo is uniquely designed to facilitate the cross fertilization of established and emerging genomic technologies, along with exciting applications. In addition, you will learn why data is the driving force that enables genomic discoveries. Next-Gen Sequencing Congress April 26th – 27th, 2011 [more...]
Date: 2010-09-29 15:13:14
IDT rises to 6th in 'Best Places to Work'
Named in the top 10 small companies for the second consecutive year Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has been named one of the top 10 ‘Best Places to Work in Industry’, in a poll of research staff conducted by a leading magazine. Published in the May issue of ‘The Scientist’, the poll put IDT 6th in the small company category, exceeding its 2009 placement of 8th. Dr. Joseph A. Walder, IDT’s founder and CEO commented, “We are pleased to be named for a second year as a leading place to work, especially since this also reflects the [more...]
Date: 2010-06-09 11:28:36
Modeling shows that factors beyond crowding affect how molecules ...
Cells are crowded with macromolecules such as proteins, RNA and DNA. Using a Brownian dynamics simulation of an Escherichia coli cytoplasm model, Georgia Tech researchers propose that crowding and hydrodynamic interactions dominate in vivo macromolecular motion. The motion of macromolecules within cells is normally random, occurring through Brownian motion that causes the molecules to diffuse through the cellular cytoplasm, which has viscosity similar to that of water. Researchers have studied the movement of fluorescent protein molecules injected into E. coli cells, but don't yet understand [more...]
Date: 2010-10-13 12:52:44
The Building Blocks of Evolution
Molecular evolution at the level of DNA, RNA, and proteins is precipitated by one or more of the following: - Base substitution, whereby one amino acid (nitrogenous base) is replaced by another. This is called point mutation. - Deletion of a group of bases. - Addition of a group of bases, e.g. if there are multiples of three, one or more amino acids may be deleted from the protein. - Inversion, or removal of a segment of DNA accompanied by reinsertion elsewhere but in inverted order. Related to inversion in a way is translocation, wherein a portion of DNA is attached to a homologous [more...]
Date: 2010-09-22 20:09:00
PhD Student for Ecology and Ecological Epigenetics in Plants ...
Recently however, evidence is accumulating that epigenetic processes, such as DNA methylation and chromatin modification, may play an important role in the short-term adaptation of plants to rapidly changing environmentats, such as are the consequence of climate change.In a European wide project (EpiCOL), considering the role of epigenetic regulation mechanisms as an alternative means towards adaptation in plants, we work on delivering “proof of principle” for the ecological and evolutionary significance of epigenetic variation. The project is a cooperation between ecologists, [more...]
Date: 2010-10-13 03:56:15
SURE Animations for Higher Biology | Citocromo P450
Posted on septiembre 14, 2010, 8:12 am, by admin, under Citocromo P450. This is a showreel for SURE Animations, a high quality 3-D animation suite for the SQA Higher Biology curriculum. The software contains 20 fully annotated animations with additional detailed descriptions of the physical processes. The animations cover: Cell types, Cell absorption, Diffusion and osmosis, Plasma membrane fluid mosaic model, Active transport, Glycolosis, Mitochondrial structure, Krebs’ cycle, Cytochrome system, Photosynthesis: Light-dependent stage, Photosynthesis: Calvin cycle, Antibody, DNA, DNA and RNA, [more...]
Date: 2010-09-14 08:12:34
Elevated Cervical White Blood Cell Infiltrate Is Associated with ...
Throughout the study, genital HIV RNA and DNA were detected in each person. Combined genital HIV (RNA and DNA) was detected at 49.2% of study visits and was associated with an elevated concentration of cervical white blood cell infiltrate (odds ratio, 2.52 ; ). Infiltrate was not associated with a clinical disorder or patient‐reported symptoms. Conclusions.  Despite antiretroviral therapy adherence and clinically suppressed plasma viremia, HIV was intermittently detected in genital secretions and was associated with [more...]
Date: 2010-10-13 00:54:32
How Can Diet Affect Sperm Quality?
Zinc and folic acid, play a role in the synthesis of DNA and RNA. Deficiency of zinc is as a clinical symptom of reduced spermatogenesis and reduced fertility. Zinc is found in shellfish and lean meat, and folic acid in dark green leafy vegetables and fortified cereals What dietary factors reduce sperm quality? Along with diet, some other factors in the lifestyle of the man may be responsible for infertility. Obesity: The increased body fat binds free testosterone in the blood, and probably favors the conversion of testosterone to female hormone estrogen. Inspect and maintain your weight in a [more...]
Date: 2010-10-11 04:51:49
Research Progress Report I
RNA-seq utilizes high-throughput DNA sequencing method for mapping and quantifying transcriptomes (the complete set of transcripts in a cell). In this method, RNA is converted to complementary DNA fragments. Each molecule is sequenced in a high-throughput way to obtain short sequences from one or both ends. 30-400 base pairs are read, and these reads are then aligned to a reference genome, a reference transcript, or “de novo,” without the genomic sequence. This yields a genome-scale transcription map that has the transcriptional structrure and shows gene expression levels. Bead [more...]
Date: 2010-10-04 01:19:26
Integrated DNA Technologies Introduces Custom Labels - Iowa City ...
Coralville Company First to Introduce Custom Labels Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, is the first company in the industry to introduce Custom Label Design to their website. With the Custom Label Design Tool, customers can tailor the information provided on their labels. By designing different custom labels, users can easily organize and manage their orders to prevent potential confusion in order receipt and retrieval. A drag-and-drop interface allows them to include information from a variety of sources including PO#, Sequence Name, Molecular [more...]
Date: 2009-06-19 18:47:55
PCR Rap | sciencerapper
An ingenious technique called Polymerase Chain Reaction (PCR, in the labaratory vernacular) uses a Polymerase to selectively an cheaply amplify certain sequences of DNA from very small amounts of starting materials. The invention of PCR piggy-backe don many existing technologies, and we have used PCR to learn many, many things about biology. The following rap about the Polymerase Chain Reaction that highlights a few things: the beautiful way in which DNA is copied (using enzymes called Polymerases) the great insight of Kary Mullis to create a useful tool that could copy DNA in monumental, [more...]
Date: 2010-09-04 14:18:04
تقنية البلمرة الجزيئية » تعرف عن عملية استخلاص شريط الحمض النووي ...
The VX Viral DNA/RNA Purification CorProtocol™ 25101 allows for walk-away automated preparation of low copy viral DNA/RNA from low total nucleic acid-yielding samples. Extracted viral DNA/RNA is of high quality and suitable for a wide variety of downstream applications. The default sample volume is 200 µL but can be scaled up to 500 µL with the use of adjustments to this protocol coupled with an alternative run file ( VX_RNA_DNA_CorProtocol_250_500ul_25101_06.CAS4 ) on the instrument. Increased volumes of certain reagents are required for the extraction of sample volumes greater [more...]
Date: 2010-07-09 16:20:26
DNA Sequencing Could Get Public-Market Boost - Venture Capital ...
Its technology is also not limited to DNA sequencing, according to the company. Other potential markets include the study of chemical and structural modifications of DNA, and the processing of RNA and proteins, according to its filing. Pacific Bio’s net loss for the first six months of 2010 was $63 million, up from $35.1 million in the first half of 2009, according to its S-1 filing. Net losses for 2009 were $87.7 million. As of June 30, it had an accumulated deficit of $255 million. It had revenue of $1.2 million for the first six months of this year, compared to none for the corresponding [more...]
Date: 2010-08-17 20:41:11
Species Nutrition Isolyze, Chocolate Milk, 4-pound Tub
This procedure has the added benefit of preserving 3 valuable components of whey immunoglobins, lactoferrins, and glycomacropeptides that are necessary for optimal immune system function and protein synthesis reactions. And if all this werent enough, ISOLYZE tastes great too! Gone are the days of the chemical- or sour-tasting whey isolates! With ISOLYZE, you get the best of both worlds great quality and great taste! List Price: $ 99.99 Price: $ 63.70 Vegan Sublingual B-12 – 90 tabs,(Deva Nutrition) Category: Vitamin B & B Complex Low Carb, Low Sodium, Dairy Free, Gluten Free, Vegan, Wheat [more...]
Date: 2010-10-12 07:29:47
The Godless Geek Blog: Titan's Atmosphere Could Be Producing the ...
There are many theories about how exactly life began on our planet. The most commonly held belief of course, is that life began in the oceans. But a recent experiment using radio waves to simulate the effects of ultraviolet radiation from the sun striking the top of Saturn's largest moon Titan's thick atmosphere, has shown that life may actually have begun in the sky. The experiment showed that when the ultraviolet radiation strikes Titan's atmosphere it can break apart molecules in the air, like molecular nitrogen and methane, leading to the production of amino acids and the nucleotide bases [more...]
Date: 2010-10-11 02:02:00
The Aspergillus Website blog: New Research to Help Identify ...
those that target DNA/RNA synthesis e.g. flucytosine. Mammalian cells make extensive use of DNA & RNA so this drug lacks specificity. Difficult to use. Clearly the best existing target in terms of specificity is the cell wall. In this news report it states that this research group have just announced the $1.5 million funding of new research into drugs that will act on a component of the construction of cell walls (UDP-galactopyranose mutase) in fungi with the aim of disrupting the process and thus making it very difficult for the fungus to make its own cell wall and grow normally. This [more...]
Date: 2010-09-29 10:13:00
What are gel elctrophoresis and DNA microarray? How is each used ...
Question by neonpinkispink: What are gel elctrophoresis and DNA microarray? How is each used in the study of genetics? Best answer: Answer by brainybroad269 Gel electrophoresis is the use of a gel to separate pieces of DNA (or RNA, or protein) under an electric field. The simplest form separates pieces by size. More complex (2-dimensional) techniques can separate proteins by size and charge. A DNA microarray is a collection of short DNA sequences (“oligonucleotides”) immobilized on a piece of glass or other support (the “gene chip”). When you incubate the chip with your experimental [more...]
Date: 2010-09-25 04:09:26
DNA Polymerase and other factors
DNA polymerase can add free nucleotides to only the 3’ end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, therefore, needs a primer at which it can add the first nucleotide. Primers consist of RNA and DNA bases with the first two bases always being RNA, and are synthesized by another enzyme called primase. An enzyme known as a helicase is required to unwind DNA from a double-strand structure to a [more...]
Date: 2008-07-27 11:03:00
Kinked nanopores slow DNA passage for easier sequencing
(Nanowerk News) In an innovation critical to improved DNA sequencing, a markedly slower transmission of DNA through nanopores has been achieved by a team led by Sandia National Laboratories researchers. Solid-state nanopores sculpted from silicon dioxide are generally straight, tiny tunnels more than a thousand times smaller than the diameter of a human hair. They are used as sensors to detect and characterize DNA, RNA and proteins. But these materials shoot through such holes so rapidly that sequencing the DNA passing through them, for example, is a problem. In a paper published this week [more...]
Date: 2010-08-02 15:18:18
Reconstructing human appearance from DNA -- Signs of the Times News
This is a relatively robust, but not foolproof, gender test, according to Kayser. Hair colour prediction underway Beyond gender, the human traits with highest predictive accuracy are those affected by pigmentation, simply because they are the least complex. Red hair colour, which is determined by a single gene, can be predicted with a certainty of around 93%. For other hair types, accuracy is slightly lower, but still promising. Kayser reported confidence levels as high as 85, 81 and 80% for predicting black, brown and blond hair respectively. Perhaps the most progress has been made in [more...]
Date: 2010-09-28 13:51:40
20000 Genes Under the Brain: Genes->Brain->Man
To assess gene expression in each small bit of tissue, researchers expose its RNA to a gene chip, or DNA microarray. These small devices are coated with clusters of identical DNA molecules, called probes, within separate areas. Each probe binds to the RNA of a specific gene—the one that contains a complementary set of chemical units, or bases. In a DNA molecule, the base adenine (A) sticks to thymine (T), and guanine (G) pairs with cytosine (C). Thus, the sequence that would bind to the strand illustrated above is (from top to bottom): T, C, C, T, G, C, A. In this way, a chip records which [more...]
Date: 2010-09-02 16:33:00
CIENCIASMEDICASNEWS: Researchers report MALAT1 gene regulates pre ...
Researchers report this month that MALAT1, a long non-coding RNA that is implicated in certain cancers, regulates pre-mRNA splicing - a critical step in the earliest stage of protein production. Their study appears in the journal Molecular Cell. Nearly 5 percent of the human genome codes for proteins, and scientists are only beginning to understand the role of the rest of the "non-coding" genome. Among the least studied non-coding genes - which are transcribed from DNA to RNA but generally are not translated into proteins - are the long non-coding RNAs (lncRNAs). Before the human genome was [more...]
Date: 2010-10-01 22:18:00
Chlorella premium superfood delivers potent nutrition ...
Chlorella is abundant in RNA and DNA. Numerous research projects in the USA and Europe indicate that Chlorella may bind to persistent hydrocarbon and metallic toxins such as DDT, PCB, mercury, cadmium and lead. In Japan, interest in Chlorella has focused largely on its detoxifying properties. The fibrous materials in Chlorella may also help support healthy digestion. Analysis shows that Chlorella Pyrensoidosa contains a comparable variety of minerals, vitamins and amino acids to other algae: Chlorella is a plant with 5 to 10 times the chlorophyll content of any other algae. How is the Cell [more...]
Date: 2010-10-13 13:00:09
Michael Shermer: Why Darwin Matters | My Secret Projects
@voncello As for viruses: They're mostly just floating bits of DNA or RNA that sometimes cause problems when they screw up some cell's DNA or RNA pattern. Other times they help increase genetic diversity. So, virus “evolution” might [more...]
Date: 2010-10-13 04:09:42
Welcome to Amino Acids: Amino Acid Translation
The process of copying genetic information from DNA into RNA is called____ protein synthesis replication transcription duplication translation DNA synthesis ___is unique to DNA. adenine cytocine uracil thymine guanine A mRNA sequence with 30 nucleotides codes for______ 15 amino acids 90 amino acids 10 amino acids 3 amino acids Translation is a process which converts the information in ____into ___ "DNA, mRNA" "mRNA, DNA" "protein, DNA" "mRNA, protein Get the answers... Missense mutation in which part of KRT5 gene does epidermolysis bullosa with mottled pigmentation form? (Answers: 1) [more...]
Date: 2008-01-19 08:00:00
Genomics and Gene Sequencing IPOs Booming?
The Company was founded in 2005, has 159 employees, and has a proprietary DNA sequencing platform with integrated software and bioinformatics providing services for genomic based research (see Raygent profile). Applications for the complete genome sequencing services are cancer research, Mendelian Disease research, Rare Variant Disease research, and clinical trial optimization. The Company has raised $95.4M since inception through six VCs and 5% shareholders: Orbimed Advisors, Essex Woodlands Health Ventures, Prospect Venture Partners III, OVP Venture Partners, Enterprise Partners and [more...]
Date: 2010-10-05 04:13:00
UC Davis News & Information :: Brain Gene Shows Dramatic ...
In a computer-based search for pieces of DNA that have undergone the most change since the ancestors of humans and chimps diverged, "Human Accelerated Region 1" or HAR1, was a clear standout, said lead author Katie Pollard, assistant professor at the UC Davis Genome Center and the Department of Statistics. "It's evolving incredibly rapidly," Pollard said. "It's really an extreme case." As a postdoctoral researcher in the lab of David Haussler at UC Santa Cruz, Pollard first scanned the chimpanzee genome for stretches of DNA that were highly similar between chimpanzees, mice and rats. Then [more...]
Date: 2006-08-16 07:00:00
Integrated DNA Technologies Receives $12.0M of Senior Subordinated ...
Integrated DNA Technologies, Inc., a Coralville, Iowa-based supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development, has received $12.0m of senior subordinated debt from Golub Capital. IDT's business is the manufacture of custom, synthetic DNA and RNA oligonucleotides and genes. Today, the company synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT's manufacturing locations include facilities in Coralville, [more...]
Date: 2010-04-26 09:25:00
Cytoplasmic & Nuclear RNA Purification Kit
RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Bio-Synthesis's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Bio-Synthesis's PCRSizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as the that from the RNA transcript. The lack of a product in lanes 1 and 3 indicate that no genomic DNA contamination is present in [more...]
Date: 2010-08-16 04:58:00
MicrobeWorld - 10 Tips for the Isolation of High Quality RNA from Soil
RNA Isolation from soil is one of the most difficult applications we perform in environmental molecular biology. RNA purification is always an arduous task and from soil it becomes a bigger challenge. One of the biggest problems is the yield of RNA from soil. Because typical yields of RNA are so much lower than for DNA, usually between 10-20% of the yield of DNA, starting with a larger amount of sample is desired. This requires a method that uses larger and slower processing methods. The other major issue is, of course, the humic acid and inhibitor content of soil co-contaminating the RNA. [more...]
Date: 2010-03-24 05:22:52
Medical Transcription From Home: What Is The Difference Between ...
DNA replication is the process by which one double helix of DNA is duplicated into two identical double helices which are also identical to the mother DNA. Translation is taking the RNA just made and using it to make a protein. Translation takes place after transcription of the genetic code into mRNA has been started. Translation refers to protein synthesis, in which proteins are made by reading the sequence information after the mature and processed mRNA by a ribosome. To me, transcription still refers to transcribing from a recording. But I thought you might want to find out why you’re [more...]
Date: 2010-08-05 18:01:00
- “Paternity Testing”
Paternity testing results? - Yahoo! UK & Ireland Answers Does anybody know how reliable dna testing was in 1982?, could it have … Seriously i think you would be better to ask a Medical Doctor, rather then random ppl … uk.answers.yahoo.com/question/index?qid… Advanced Testing Center - 10 Commercial St , Foxboro, MA 02035 … … igm test pcr test hiv rna test igg testing igm testing pcr testing dna test dna testing confidential herpes testing paternity test paternity testing. … www.getfave.com/12341786-advanced-testing-center court- ordered paternity testing in michigan? how would [more...]
Date: 2010-04-29 23:36:17
Complete DNA Sequencing & Synthesis Services,DNA Sequencing Analysis System. Custom DNA Synthesis Quick Turnaround w/ 100% Accuracy Guaranteed Expression available. AltaVista found 18,300,000 results DNA replication DNA polymerases, isolated from cells, and artificial DNA primers are used to initiate DNA synthesis at known sequences in a template molecule. … How is dna synthesis made DNA polymerase is the enzyme that catalyzes the reactions involved in … After DNA synthesis is complete, the DNA is typically isolated and purified. … DNA and Protein Synthesis DNA and Protein [more...]
Date: 2010-06-13 03:43:08
Taking a stride toward synthetic life | Harvard Gazette Online
“The reason it is a step toward artificial life is that the key component of all living systems is the ribosome, which does protein synthesis. It is the most conserved and one of the most complicated biological machines,” Church said. ... His work, he said, is exploring the properties of these RNA-like molecules, seeking variations that make them better early candidates to store and replicate genetic information than either DNA or RNA, which perform those functions in [more...]
Date: 2009-03-08 00:29:28
IntegenX Awarded DTRA Contract to Develop Pathogen Detection and ...
The microfluidics technology platform integrates advanced fluidics and analytical robotic capabilities to produce sample-to-answer performance for DNA and RNA sequencing, DNA-based forensics and human identity (HID) testing, biodefense, [more...]
Date: 2010-10-08 13:00:00
What is the different between a DNA virus and a RNA virus ...
Welcome to ScienceForums.Net! We welcome science discussion at all levels from beginners to researchers, covering topics from biology to computer science, and much more. Registration is fast and free, and allows you to post on the forums, so register now and join the discussions! After you've registered, come in and introduce yourself, or visit the forum index. If you need any help registering, posting, or if you just have some questions about our site, please feel free to contact us at staff at scienceforums dot net. Start new topics and reply to othersSubscribe to topics and forums to [more...]
Date: 2009-02-18 13:59:36