Our blogs and videos choice: purification of dna

Tecan and PhyNexus collaborate to automate protein purification ...

Tecan and PhyNexus have developed standard processes for automating protein purification using PhyTip® columns integrated onto a Freedom EVO® liquid handling platform. The Freedom EVO is equipped with a liquid handling arm and a MultiChannel Arm™, and controlled using specially adapted scripts of standard Freedom EVOware® operating software. Although primarily used for protein purification, the disposable PhyTip columns can be supplied packed with various types of resin, making their application range extremely broad. The majority of PhyTip columns do not require additional hardware, the [more...]

Date: 2010-07-28 09:19:39

Blog posts (41) | Videos (25)
 


The Purification of Plasmid DNA for Clinical Trials Using Membrane ...

ABSTRACTMembrane chromatography offers a good solution to the challenge of developing an efficient chromatographic procedure for plasmid DNA purification. The large convective pores of anion exchange membranes allow plasmid DNA to access all the anionic binding sites of the membrane at high flow rates. Here we demonstrate that the pIDKE2 plasmid can be purified from a recombinant Escherichia coli lysate using a Sartobind D membrane combined with size exclusion chromatography to render material with 95% purity and an average yield of 50%. This process yields therapeutically suitable plasmid [more...]

Date: 2010-02-01 05:00:00


Octapharma licenses new BAC purification ligand

Bioprocess purification chromatography tech wins BAC new deal after Swiss protein maker Octapharma licenses it for commercial-scale manufacture. The technology in question, CaptureSelect, uses camelid-derived single domain antibody fragments to produce affinity ligands capable of capturing specific proteins during biomanufacturing. BAC claims the ligands offer increased stability and broad antigen-specific binding compared to traditional affinity purification methods. Under the new agreement, Lachen-headquartered Octapharma will use a CaptureSelect affinity ligand it developed in [more...]

Date: 2010-08-17 09:00:00


Magickal Oils of Indonesian Shamanism Part 1 —earthquakes-today ...

Once the purification process has been completed the candle may be extinguished but allow the incense be totally consumed. There is this belief that Kawiyang oil should be taken out of one’s dwelling place when a member of one’s household passes away. Only after the deceased has been buried is it permissible to return the cupu with the oil to its original place within the home. It is also said that the possessor of the Kawiyang oil should not quarrel with one’s wife or commit adultery for this would cause ill fortune. If violated one should rectify the situation by pouring the blood of [more...]

Date: 2010-10-10 05:48:38


Associate Director, Purification Process Development

... including initial method design through process scale and MFG support of existing processes. This person will be held accountable for purification process design including but not limited to initial toxicology runs, clinical [more...]

Date: 2010-09-21 23:29:35


Ridiculous Emergency Response Scenarios

That's assuming you want to hit the tank and bypass the extensive water purification process that would wash out your chemical. Using a chemical to poison a city water system is really unrealistic. But imagine my surprise to hear that, given a scenario where there are casualties resulting from poisoning the drinking water, the emergency responders want to set up a decontamination line and collect contaminated material. Exactly what are you going to decontaminate??? There is no persistent chemical hazard here, no danger of widespread exposure to additional persons, no contaminated material [more...]

Date: 2010-10-11 14:05:00


Water Purification Process – How Valuable Are The Water ...

There are several steps involved in removing the pollutants present in drinking water that's without treatment through using a water purification process. Nevertheless, detailing this completely is quite the tedious work that is why [more...]

Date: 2010-09-09 22:05:23


Sr. Development Specialist I - Downstream - Chemical Engineering

Scope of work includes a variety of downstream unit operations, such as harvest clarification, capture and recovery, various chromatography column purifications, UF/DF and normal flow filtrations etc. This individual will contribute as a key member of ... •Have excellent knowledge and experience with protein purification process development and GMP manufacturing support, including protein capture and recovery, chromatography purification, filtration, and UF/DF etc. [more...]

Date: 2010-09-27 06:00:00


Analytical Biochemist, Protein Purification and Characterization

We are seeking an experienced research associate with expertise in protein purification and bio-analytical techniques with a focus on antibodies. Demonstrated proficiency in the design, execution, optimization and troubleshooting of purification processes at the bench scale (10mg to 5g) is required. Proven experience in biochemical and biophysical characterization of proteins is essential. Ability to analyze, interpret and effectively present data at group and departmental… Naturejobs – All [more...]

Date: 2010-09-28 22:51:01


Benefits of Omega 3 Fatty Acids - MESO-Rx

It also helps in the proper working of the lungs, which means the purification process of the blood works absolutely fine. 2. Sugar levels in the body are maintained in the normal range due to the intake of the omega 3 fatty acids. It is a good solution for the diabetes. Intake of adequate amount of these fatty acids doesn’t allow the excessive sugar to get accumulated in the body. It digests the sugar content by promoting the proper functioning of the pancreas. Doctors generally recommend to the diabetes patients to eat the food items containing the omega 3 fatty [more...]

Date: 2010-10-12 09:52:21


ISPUB - Identification Of Lipase – Producing Psychrophilic Yeast ...

Lambda HindIII was used as DNA marker. After the electrophoresis, gel was stained with ethidium bromide and documented by the Gel Doc 2000 (Bio-Rad). Purification of PCR product was done by using QiAquick Gel Extraction Kit. Then, the purified PCR product obtained was sequenced and submitted for BLAST (Basic Local Alignment Search Tools) at NCBI (National Center for Biotechnological Information). Lipase assay by titrationActivity of lipase was assayed by titration using oil emulsion (polyvinyl alcohol: olive oil; 3:1) as substrate (Arima et al., 1972). The assay medium consists of 5ml oil [more...]

Date: 2010-08-25 00:51:13


Protein Chemist Mumbai, Quality Control Manager : Yashraj ...

The candidate is expected to be proficient in protein isolation and purification tools. The incumbent must have thorough knowledge and experience in protein chemistry, protein isolation and purification from large volumes of human biological fluids and validation, protein refolding and process design. The applicant should have publications in international journals as an evidence of his/her expertise in recombinant protein technologies. Posts Email Id: ybresearchscientist@gmail.com Quality Control Manager Job Description: The team leader would be responsible for quality control programmes [more...]

Date: 2010-10-02 07:00:00


Automation, Robotics in the use of Plasmid purification for the ...

I am looking for scientists who have tried plasmid purification in miniprep format (96 well). We are automating many of our processes using a TECAN robot and are looking for a plasmid purification kit that produces transfection-grade [more...]

Date: 2005-10-26 19:48:46


JoVE: Electroeluting DNA Fragments (Video Protocol)

Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution from glass or silica particles, DEAE-cellulose membranes, "crush and soak method", electroelution or very often expensive commercial purification kits. Thus, selecting a method will depend mostly of what is available in the laboratory. The [more...]

Date: 2010-09-05 22:00:00


"Condemnation without investigation is the height of ignorance ...

Monday, September 27, 2010 by Mike Adams, the Health Ranger Editor of NaturalNews.com (See all articles...) (NaturalNews) It has been a few days since Abbott Laboratories, makers of Similac infant formula, issued a recall after discovering some of its products may have been contaminated with beetles (and beetle larvae). The mainstream media ran with this story and parents everywhere suddenly got concerned that their babies might be drinking beetle parts. But are beetles really the scariest thing in Similac? Not by a long shot if you know anything about nutrition. I purchased a 24-oz [more...]

Date: 2010-10-13 04:54:00


Research Associate (San Diego) | PCR Jobs

"How To Answer Any Question An Interviewer Could Possibly Throw At You! ... " Click Here! Purpose of the Position:  Responsible for research and/or development of molecular diagnostic test kits, reagents and procedures in a team environment.  Performs experiments independently after receiving general direction from supervisor. Makes detailed observations, analyzes data and interprets results.  Prepares technical reports, summaries, protocols and quantitative analyses.  Maintains and develops skills in molecular biology and company technology through reading of internal reports [more...]

Date: 2010-10-01 19:07:24


solutions manual to introduction to particle technology ...

High-Throughput Solutions for DNA Purification Using MagneSil … INTRODUCTION. MagneSilâ„¢ technology was developed specifically to meet the changing … small particle size allows rapid movement in a magnetic field, resulting … plasmid DNA in <2 hours with no manual interventions. Unique magnetic … http://www.promega.com/pnotes/75/8554_07/8554_07.pdf * pdf N07848_ver2.5 MagMAX Express User Manual Principle of the MagMAXâ„¢ Express magnetic particle processor . ….. Chapter 3: Introduction to the MagMAXâ„¢ Express …. The operating principle employed is MPP (inverse [more...]

Date: 2010-10-13 07:08:10


Expressing, purifying and characterizing proteins : PSI-Nature ...

Generally, the use of affinity tags to drive protein purification is widespread within PSI centers. Many tags can be used, but most groups use an N-terminal hexahistidine tag, partly because evidence suggests that hexahistidine tags do not normally have much effect on the N-terminal structure 4 and partly because histidine tags are small compared with other tags and less likely to affect solubility. An alternative for integral membrane proteins is to add a specific membrane-integrating fusion sequence for translation of integral membrane construct (Mistic); such fusions are proving useful for [more...]

Date: 2010-06-16 07:00:00


Protein Purification: Principles and Practice | Shanghai Xiu ...

product The third ? me? edition of this classic guide to PROTEINS betting? day of purification of m? methods, principles and r? f? differences. As in the volumes small-d? C lunch?? S br? more t t, Scopes F? S Guide? Beginners and experienced researchers? Rhyme? of th? orie? application. With the book, the reader is able to int? Grate the m? Methods in protocols optimal f? The R t? Che. Messages? GE too t t? D? Criteria in volumes pr? C? Teeth purification PROTEINS like? Good practical advice? GE, which is pr? Feel? in … more>> Protein Purification: Principles and Practice Tags: Practice, [more...]

Date: 2010-09-30 17:02:27


Antibody Purification Using Membrane Adsorbers - Results from a ...

Dec 1, 2008 By: Leonardo Giovannoni, PhD, Marco Ventani, Uwe Gottschalk, PhD BioPharm International Volume 21, Issue 12 ABSTRACT Most antibody manufacturers currently use a three-column platform comprising Protein A affinity chromatography for product capture, followed by anion exchange (AEX) chromatography in flow-through mode to extract negatively charged contaminants, and then cation exchange (CEX) chromatography or hydrophobic interaction chromatography (HIC) in retention mode to remove positively charged contaminant species. This article presents a new process that uses membrane [more...]

Date: 2008-12-01 05:00:00


Plasmid DNA Recovery Using Size-Exclusion and Perfusion ...

Finally, a second size-exclusion chromatography step was carried out to purify the plasmid DNA from other small molecular-weight contaminants. Analytical methods proved that the purified plasmid DNA had a purity of 95% after Sephacryl S1000. Plasmid identity was confirmed by restriction enzyme digestion. Biological activity of the purified plasmid was confirmed in vivo; immunized mice developed a positive antibody response against all HCV structural antigens. This procedure offers an alternative to traditional methods that use organic reagents, mutagenic and toxic compounds, and [more...]

Date: 2008-09-01 04:00:00


Water Purification Process – How Effective Are The Water ...

There are many steps involved in removing the pollutants present in water that is untreated through the use of a water purification process. Nevertheless, explaining this completely is fairly a tedious work that’s the reason we are compressing everything right into a easy outline, to ensure that you will get the common concept. This method had been around sometime ago, before technologies decided our planet. There is this organic way of purifying water. Water through rainfall enters the actual dirt and the ground water purification this as well as cleanses it as this passes via several [more...]

Date: 2010-09-09 16:15:29


Water Purification Process – How effective are the Water ...

There are several actions involved in treatment of contaminants present in drinking water that is untreated through using a water purification process. Nevertheless, detailing this completely is quite the tiresome job that is why we are compressing everything right into a simple describe, to ensure that you will obtain the common idea. This method experienced been around long ago, before technology decided the planet. There is this particular organic way of cleansing drinking water. Drinking water from rainfall enters the actual soil as well as the floor filter systems it as well as cleansing [more...]

Date: 2010-09-17 07:47:52


Water Purification Process – How Helpful Are The Water ...

There are many actions involved with removing the pollutants contained in water that is (Cheap Viagra) without treatment through the use of a water purification process. However, detailing it thoroughly is fairly the tiresome job that’s the reason we are compressing every thing right into a simple outline, to ensure that you’ll get the common concept. This process experienced existed long ago, before technologies decided our planet. There was this particular organic way of purifying water. Drinking water through rain enters the actual soil as well as the ground water filtration process [more...]

Date: 2010-09-10 22:04:31


One In That Most Useful Purification Activity: Ft . Spa

One In That Most Useful Purification Activity: Ft . Spa Everyday our body grows unseen ±nner poisons this originate emotional tension together with uninteresting each day process. Be honest! You are well being are at chances thanks to harmful makes a difference invading our ±nner body which leads in order to made weaker health and the propensity to get health conditions develops into increased warmth and well being mitigation could possibly be problematic. Your purify ankle health spas mat could certainly get the job done that you efficiency the duty of obtaining unwelcome conditions. [more...]

Date: 2010-10-13 16:15:25


Water Purification Process – How effective are the Water ...

There are several steps involved with treatment of pollutants contained in drinking water that is without treatment through the use of the water purification process. However, detailing it thoroughly is quite the tiresome job that’s the reason we’re compressing everything into a easy describe, so that you’ll get the common idea. This process had been around sometime ago, even before technology ruled the planet. There was this organic way of cleansing drinking water. Water from rainfall makes its way into the dirt as well as the ground water purification this and cleanses it as being [more...]

Date: 2010-09-30 17:32:39


HyperHep B (Hepatitis B Immune Globulin (Human)) Drug Information ...

A direct relationship between the partitioning of the pathogenic prion protein and transmissible spongiform encephalopathy infectivity during the purification of plasma proteins. Transfusion 2001. 41(4):449-55. 24. Lee DC, Stenland CJ, Hartwell RC, et al. Monitoring plasma processing steps with a sensitive Western blot assay for the detection of the prion protein. J Virol Methods 2000. 84(1):77-89. 25. Cai K, Miller JL, Stenland CJ, et al. Solvent-dependent precipitation of prion protein. Biochim Biophys Acta 2002. 1597(1):28-35.Last reviewed on RxList: 11/11/2008 Acute Exposure [more...]

Date: 2008-11-11 07:00:00


BIO-SYNTHESIS - Custom Antibody Custom Peptide Synthesis Custom ...

BIO-SYNTHESIS, INC. is a leading life science products company with over 20 years of experience in the design and synthesis of Custom Peptide, small molecules and reagents for small scale research and bulk pharmaceutical trials. Using state of the art technology in our well-equipped laboratories. gp120 Antibody Catalog# : 4777 Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, [more...]

Date: 2010-10-13 10:37:00


Do You Know How Municipal Water Purifying Systems Purify Water ...

Many municipal water treatment systems utilize a 3-stage process. In the primary stage, sewage is stored in tanks where the heavy solids may sink to the base. This enables the lighter components, like grease as well as oil, to rise up. After getting rid of all the solids which have built up in the base of the tank as well as the lighter ones on top, the water is prepared to be directed on to the second phase of the procedure. The second treatment the wastewater goes through removes all the biological matter that is either suspended or dissolved in it. At this phase indigenous microorganisms [more...]

Date: 2010-10-13 12:45:32


Quick and reliable DNA purification and sequencing

Keep up with the latest research, events and funding news. Add the  observer,  dissemination and  funding widget to your website or blog The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems.  Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting Station, a high-speed precision pipettor. With its versatile shelf configuration options, the [more...]

Date: 2010-07-28 13:03:49


Quick & Reliable DNA Purification and Sequencing…

BioCel DNA Purification and Sequencing Royston, UK -- An applications bulletin is available from Agilent Automation Solutions that describes an automated system that provides for quick and reliable DNA purification and sequencing. The demand for quick and reliable DNA sample preparation for sequencing has risen dramatically during recent years. The long and complex pipetting protocols needed for DNA preparation have been a challenge for many lab automation systems. Agilent Automation Solutions met this challenge by designing a BioCel system based around the Agilent Vertical Pipetting [more...]

Date: 2010-08-14 14:00:00


Pharma & Biotech Global Week in Review 29 September 2010 from IP ...

US: Genentech seeks declaratory judgment of non-infringement of ‘antibody purification’ patents (Patent Docs) US: Senators vigorously object to the inclusion of patent settlement provisions in FY 2011 Appropriations Bill (FDA Law Blog) US: A high wire balancing act: FDA and CMS to consider parallel review of medical products (FDA Law Blog) US: Reform of the de novo classification process needs to be a top priority (FDA Law Blog) USPTO looking for ways to incentivize humanitarian technologies (Patent Docs) US: Biosimilar approval pathway: Who will be first? (Patent Docs) US statement to [more...]

Date: 2010-09-28 22:14:01


Industrial Protein Purification

Theresa's Biotech / Biomedical BlogBy Theresa Phillips, Biotech / Biomedical GuideMy BioMy BlogMy ForumRSS Pall Life Sciences, I've learned that methods for purifying a protein from a large-scale fermentation are not much different from methods in the lab. The main difference really seems to be the cost of scaling up processes, since, obviously, the prospect of using an expensive buffer or other media in the lab is much less daunting than if you're talking about 10,000 litres of the stuff. According to Jason, anything you can do in the lab you can do at large scale, but it's a matter [more...]

Date: 2010-06-10 06:30:29


GSK sues Roche for patent infringement - FiercePharma

The U.K. drugmaker is suing Roche, alleging that its production of Herceptin infringes its patents covering "antibody purification." And Genentech immediately fired back with a lawsuit of its own, asking a U.S. judge to affirm that it hasn't stepped on GSK's patents. At issue are two patents GSK obtained in 2008 and last month, Bloomberg reports. Apparently, they cover a particular process that GSK believes to be used during Herceptin manufacturing. Roche and Genentech infringe the patent by "making and/or having made therapeutic antibody products, including without limitation Herceptin," [more...]

Date: 2010-09-22 14:14:40


DNA Art Blog » Blog Archive » PCR Process

We use primer sets that are optimally designed to span the specific genes you choose to have isolated for your canvas. Our technique focuses on the prevention of pre-PCR mis-priming and primer dimerization. Our primers are designed using software with algorithms that check for the potential of secondary structure formation and annealing of primers to itself or within primer pairs. We use hot start Taq Polymerase is designed to enhance the specificity, sensitivity, and yield of the target DNA sequence. Following PCR amplification and purification of the template DNA, we use restriction [more...]

Date: 2010-10-12 15:19:03


Nucleic Acid Separation

The separation of mRNA from different varieties of RNA wants a special strategy. The Nucleic Acid Separation beads strategy is being used in this separation technique. The isolation and Nucleic Acid Purification are very a lot important for the delicate reactions. The purification is essential for several experiments. We need pure mRNA for doing RT PCR. The RT is nothing however the technology of DNA from the mRNA utilizing the viral enzyme reverse transcriptase. The newly introduced system of protein sure with magnetic beads shall be very helpful in addition to yield very pure particles of [more...]

Date: 2010-09-30 12:19:53


MicrobeWorld - 10 Tips for the Isolation of High Quality RNA from Soil

RNA Isolation from soil is one of the most difficult applications we perform in environmental molecular biology. RNA purification is always an arduous task and from soil it becomes a bigger challenge. One of the biggest problems is the yield of RNA from soil. Because typical yields of RNA are so much lower than for DNA, usually between 10-20% of the yield of DNA, starting with a larger amount of sample is desired. This requires a method that uses larger and slower processing methods. The other major issue is, of course, the humic acid and inhibitor content of soil co-contaminating the RNA. [more...]

Date: 2010-03-24 05:22:52


Plasma Protein Purification Technician (1245)

Participates in manufacturing of cGMP plasma protein products by preparing chemicals, buffers, and operating equipment used in the plasma manufacturing process. Must work well in a team environment. Cleans, sanitizes and cares for [more...]

Date: 2008-08-07 12:00:00


Water Purification Process – How great are the water purification ...

Essentially, in the event that we should know of the water purification process, we need to understand first what we tend to be precisely dealing with the following. What exactly are all of us attempting to water purifying from? Just what should we end up being removing in water to make it genuine? Nicely, there are many different things that we need to remove through drinking water, chemicals, bacteria along with other pollutants that originated in the ground by itself because of the air pollution which was removed through various resources, mainly human being created. These pollutants made [more...]

Date: 2010-09-15 13:33:00


Economic Drivers and Trade-Offs in Antibody Purification Processes ...

ABSTRACTIncreasing titers in mammalian cell culture has recently shifted the focus of process development efforts toward improving the economics of product recovery and purification processes. Some argue that a paradigm shift may be required to ensure timely and cost-effective delivery of future antibody candidates. This paper discusses the key process economic drivers and the impact of scale and titer on downstream processing (DSP) economic trade-offs. The success of therapeutic monoclonal antibodies (MAbs) in the treatment of indications such as cancer and autoimmune diseases has fuelled [more...]

Date: 2009-03-02 05:00:00


Clean Genes: Michigan Tech Chemist Culls the Good Synthetic DNA ...

Synthetic DNA purification process developed by Shiyue Fang, Michigan Tech Chemistry Department image July 22, 2010— Birds do it, bees do it. Even scientists in labs do it. But the scientists can’t hold a candle to the birds and the bees, who can make gobs of primo DNA without even thinking about it.DNA is a critical element of gene therapy, and scientists working to develop cures for diseases make it with synthesizers. Unfortunately, synthesizers don’t do nearly as good a job as cells in stringing nucleic acids together to make DNA, and many of the resulting sequences—called [more...]

Date: 2010-07-22 19:46:37



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