Gene Sequencing: DNA analysis
Not everyone can properly and correctly analyze the DNA. Apartyears of study devoted to knowledge of DNA, DNA analysts to go through years of experience and practice in laboratories and practice of DNA testing and analysis in order to analyze the DNA and how it works. The training of DNA analysts understanding of sophisticated tools and machines developed for DNA analysis. proper management and use of these devices would make them more efficient in the pursuit of DNA to be analyzed correctly. Most of the DNA consists of 23Pairs of chromosomes, mitochondrial DNA inherited from the mother, and [more...]
Date: 2010-09-22 05:00:00
Scientists say rare plant has biggest genome yet « Xenophilia ...
A genome is the full complement of an organism’s DNA, complex molecules that direct the formation and function of all living organisms. The size of an organism’s genome is typically measured by the number of bases it contains – base pairs being the building blocks of DNA. The human genome, for example, has about 3 million bases and measures about 6 feet in length. The marbled lungfish has a whopping 130 million bases. And the 12-inch (30-centimeter) flower studied by Leitch turns out to have 150 million. Outside experts were impressed. “This is certainly an enormously large genome,” [more...]
Date: 2010-10-08 08:37:15
DNA strides into organic synthesis
The walker and three 'stations', made from specific DNA sequences, organise themselves along the track using the normal pairing of DNA bases. 'The walker will move unidirectionally along the track,' Liu says. 'We attached chemical reactants to the walker and each station on the track. As the DNA walker moves from station to station, a programmed series of chemical reactions takes place.' The walking strand of DNA moves to each station (blue, red and green) in turn and reacts with the attached reagent © Nature Nanotechnol. In this way, the Harvard team was able to perform three acylation [more...]
Date: 2010-10-10 22:46:00
What Does DNA Stand For in Science | Intelligent Mag
Before cells are divided, the chromosomes duplicate first; this is what we call replication of DNA. This is the division of cells which results in two cells that have to contain the same information like their mother-cell. There are four nitrogenous bases within the DNA molecule. Those are: Adenine, Cytosine, Thymine and Guanine. Genes are composed of bases. In case of DNA damaging, the sequence of DNA will be changed. This happens when there is mutagen factor involved. These mutagens are highly radioactive. The damage that occurs as a result of the radiation is determined by the mutagen [more...]
Date: 2010-10-09 19:47:49
The effect of DNA signals on nucleosome formation, chromatin ...
Much of the human genome is non-coding and it seems likely that at least some of the non-coding DNA is functionally important. However, only little is known about the possible function of the bulk of the human genome. Since essentially all the nuclear DNA is packaged into chromatin, it is likely that functional effects of non-coding DNA are mediated by the chromatin structure. By making use of human genomic sequence information now available, we showed that a specific set of periodic DNA motifs (period-10 VWG/CWB) encoded in genomic DNA is predicted to influence human chromosome function. The [more...]
Date: 2010-08-25 23:15:17
Casey Anthony Team Gets “Touch DNA” Testing | The Caylee Daily
Recent Comments hopeful for justice (Wash Pa) on North Carolina Stepmother of Missing Girl Zahra Appears in CourtMaggie on Anthony Defense Gets OK To Look At Coffin Flies, Caylee’s HairErica on Anthony Defense Gets OK To Look At Coffin Flies, Caylee’s HairMorgandy on North Carolina Stepmother of Missing Girl Zahra Appears in CourtPete86047 on Rescue of 33 Trapped Chilean MinersPeggy@Al on Anthony Defense Gets OK To Look At Coffin Flies, Caylee’s Hairshoozeyque on Anthony Defense Gets OK To Look At Coffin Flies, Caylee’s Hair ORLANDO SENTINEL: The judge in the Casey Anthony case [more...]
Date: 2010-09-29 09:20:31
Heliscope builds world's fastest DNA sequencer | Singularity Hub
The Short: Technology Review reports that for just $1,350,000 USD you can have have the worlds fastest commercially available DNA sequencer, called the Heliscope. The machine developed by Helicos BioSciences takes just one hour to read 1.3 billion base pairs from a strand of DNA. Here is a picture of the beauty: HeliScope™ Single Molecule Sequencer The Long: The Heliscope is being marketed as a DNA microscope. It is unique in the field of DNA sequencing because it sequences an actual DNA strand whereas most other sequencing technologies use PCR to create millions of copies of an original [more...]
Date: 2008-07-14 16:58:33
DNA or RNA friendship necklaces - Boing Boing
Genes are written in DNA and RNA molecules using a code of chemical units called bases. These bases form pairs, which is critical to their function. In DNA, A (adenine) pairs with T (thymine) and G (guanine) pairs with C (cytosine). In RNA, G pairs with C and A pairs with U (uracil). The charms are based on shapes of the base molecules. They are strung onto a chain at the place where they would normally connect to the sugar-phosphate backbone. This set of two necklaces comes with your choice of A-T, A-U, or G-C. The charms are about 3/4 inches long, are made with high-quality [more...]
Date: 2010-07-12 18:49:05
DNA art imitates life: Construction of a nanoscale Mobius strip
The circulations of earth's warmer and cooler ocean currents for example, describe a Möbius shape. Other topological structures are common to biological systems, particularly in the case of DNA, the 3 billion chemical bases of which are packed by the chromosome inside the cell, using topological structures. "In bacteria, plasmid DNA is wound into a supercoil," Yan explains. "Then the enzymes can come in and cut and reconfigure the topology to relieve the torsion in the supercoil so that all the other cellular machinery can have access to the gene for replication, transcription and so [more...]
Date: 2010-10-08 21:50:00
Xiang-Jun's Corner: Further details of the DNA story revealed by ...
I read Watson's "The Double Helix" book a while ago, and overall I am quite familiar with the DNA story. Still, I found this account fascinating: it provides a "CAST LIST" in "The search for the structure of DNA" with photos (p521); and it succinctly summarizes the relationships among the key players. In science, no other story shows more dramatically the collaborative and competitive nature among scientists working on similar projects. Franklin’s X-ray diffraction photograph 51 of B-form DNA (see figure above, from Wikipedia), with its unambiguous evidence that DNA was helical, proved [more...]
Date: 2010-10-01 03:42:00
The cell nucleus – a mystery of evolution | DNA Sequencing
For example, eachDrosophila nucleus contains four pairs of chromosomes, each containing 23 pairs of human core. Chromosomes consist of DNA-histone proteins as a wound coil wire wound. If this DNA is wrapped around the genes of genes, those copied into RNA, which contain DNA. The soul itself is a double membrane separating the contents enclosed by the nuclear cytoplasm of the cell keeps the rest. This double membrane is studdedlet certain molecules pass through pores. RNA copies of genes, for example, pass through the pores from the nucleus to the cytoplasm e. There are activities for the [more...]
Date: 2010-10-06 14:50:15
DNA Testing - Legal DNA Tests DNA Testing Center Paternity Test ...
DNA Identity Testing Center is a leading provider for Legal DNA Test, Legal DNA paternity testing, Immigration DNA Testing, Infidelity Detection and DNA testing services. Incoming freshmen at UC Berkeley will receive a cotton swab kit in the mail to participate in voluntary DNA testing as university-wide project. This will be the first mass genetic testing by a university looking for three specific genes as following: 1- Ability to break down alcohol 2- Difficulty to digesting dairy products 3- Regulating body’s ability to absorb the vitamins contained in green, leafy vegetables Despite the [more...]
Date: 2010-09-13 17:51:00
Genes | Free Full-Text | The Application of Next Generation ...
Recently various high-throughput approaches based on bisulfite conversion combined with next generation sequencing have been developed and applied for the genome wide analysis of DNA methylation. These methods provide single base pair resolution, quantitative DNA methylation data with genome wide coverage. We review these methods here and discuss some technical points of special interest like the sequence depth necessary to reach conclusions, the identification of clonal DNA amplification after bisulfite conversion and the detection of non-CpG methylation. Future application of these methods [more...]
Date: 2010-06-04 07:00:00
Free DNA Testing to students at UC Berkeley
Despite the recent headlines for home DNA test designed for paternity and relationship analysis, people behind the university effort beleieve this should yield some exciting results. Jasper Rine, professor of genetics at the university and the faculty member who is leading the project said " this is an educational project, not a research project". Once the samples are returned, they will be sent to the private laboratory for testing and the reults will be available in about one week. Participant must attend Rine's public lecture on September 13 in order to get their results. The program will [more...]
Date: 2010-09-16 14:56:00
Witness The World's First Double Helix Bridge In Sunny Singapore
Designed to resemble the helicoidal structure of human DNA, the structure has been built almost entirely out of corrosion-free stainless steel that also features state-of-the-art computer controlled mood lighting once dusk sets in. Built according to the vision of the Australian company Cox Group, Singapore’s own Architects 61 and engineering outfit Arup the structure also features nearly 2,250 metre long steel tubes winding in opposing directions. The connection with DNA is also denoted from the two pairs of letters that appear in green and red on the bridge facade once the sun goes down. [more...]
Date: 2010-10-11 09:36:54
PCR Rap | sciencerapper
An ingenious technique called Polymerase Chain Reaction (PCR, in the labaratory vernacular) uses a Polymerase to selectively an cheaply amplify certain sequences of DNA from very small amounts of starting materials. The invention of PCR piggy-backe don many existing technologies, and we have used PCR to learn many, many things about biology. The following rap about the Polymerase Chain Reaction that highlights a few things: the beautiful way in which DNA is copied (using enzymes called Polymerases) the great insight of Kary Mullis to create a useful tool that could copy DNA in monumental, [more...]
Date: 2010-09-04 14:18:04
Dienekes' Anthropology Blog: Y-STR mega-paper (Ballantyne et al. 2010)
To rectify this, we analyzed a large number of 186 Y-STR markers in nearly 2000 DNA-confirmed father-son pairs, covering an overall number of 352,999 meiotic transfers. Following confirmation by DNA sequence analysis, the retrieved mutation data were modeled via a Bayesian approach, resulting in mutation rates from 3.78 × 10−4 (95% credible interval , 1.38 × 10−5 − 2.02 × 10−3) to 7.44 × 10−2 (95% CI, 6.51 × 10−2 − 9.09 × 10−2) per marker per generation. With the 924 mutations at 120 Y-STR markers, a nonsignificant excess of repeat losses versus gains (1.16:1), as [more...]
Date: 2010-09-05 12:00:00
Chernobyl: a DNA discovery on substitution rates
Chernobyl species decline linked to DNA Victoria Gill writes about the following paper: Historical mutation rates predict susceptibility to radiation in Chernobyl birds (full text PDF) written by A.P. Moller, J. Erritzoe, F. Karadas, and T.A. Mousseau affiliated with French, Norwegian, Danish, Turkish, and American institutions. They wanted to figure out which species are going to be affected by events of the Chernobyl type - that clearly increase the mutation rates etc. Which of them will see their abundance decline quickly? The answer sounds logical but it's always helpful to confirm a [more...]
Date: 2010-08-23 04:50:00
SpringerProtocols: Abstract: The State-of-the-Art of Chromatin ...
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the DNA sequences associated with it. ChIP has been widely used to map the localization of post-translationally modified histones, histone variants, transcription factors, or chromatin-modifying enzymes on the genome or on a given locus. In [more...]
Date: 2009-08-01 04:00:00
Starchild Skull 2010 DNA Result - Earth Changes and the Pole Shift
Part of the nuclear DNA have can thus reduced to its ( expected ) of human origin, while have surrendered to others subsections at all , no significant similarities and similarities to other earthly creatures. That is, the genotype of the father was definitely not found in the database of the National Institutes of Health (NIH ) in Maryland. The NIH is the world 's largest database of genetic information of many species of viruses , bacteria , crustaceans, fish to all types of plants and animals , including primates and humans. These results of DNA analysis were repeated several times and [more...]
Date: 2010-09-26 08:31:32
DNA sequencing » Blog Archive » DNA Analysis
DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a DNA profiling involved restriction enzyme digestion, followed by Southern blot analysis. DNA fingerprint: Definition from Answers.com DNA fingerprint n. An individual’s unique sequence of DNA base pairs, determined by exposing a sample of the person’s DNA to molecular [more...]
Date: 2010-08-31 16:03:47
DNA methylation analysis of multiple tissues from newborn twins ...
DNA methylation analysis of multiple tissues from newborn twins reveals both genetic and intrauterine components to variation in the human neonatal epigenome Miina Ollikainen1,2, Katherine R. Smith3, Eric Ji-Hoon Joo1,2, Hong Kiat Ng1,2, Roberta Andronikos1,2, Boris Novakovic1,2, Nur Khairunnisa Abdul Aziz1,2, John B. Carlin2,3, Ruth Morley1,2, Richard Saffery1,2,* and Jeffrey M. Craig1,2,* 1Developmental Epigenetics, Murdoch Childrens Research Institute, Royal Children’s Hospital, Parkville, Victoria 3052, Australia 2Department of Paediatrics, University of Melbourne, Parkville, [more...]
Date: 2010-08-10 12:59:39
My Weblog on Bioinformatics, Genome Science, Next Generation ...
Just saw this paper Sequencing and analysis of an Irish human genome. AFAIK WGS is usually done at 30x coverage. In this paper, the authors “describe a novel method for improving SNP calling accuracy at low genome coverage using haplotype information.” I thought it was pretty good considering that they had 99.3% of the reference genome covered for 10.6x coverage. That leaves only like 21 Mbases missing .. For those interested in the tech details Four single-end and five paired-end DNA libraries were generated and sequenced using a GAII Illumina Genome Analyzer. The read lengths of the [more...]
Date: 2010-10-12 10:49:00
How Do I separate 2 DNA fragment of similar length on an ...
Question by MrBurlish: How Do I separate 2 DNA fragment of similar length on an electrophoresis gel? One is 2600 base pairs and one is 2700 base pairs Best answer: Answer by Suzy Since you have the DNA fragments already cut that means you’ve already used an enzyme to do so. After that, you use the micropipet to insert the DNA into the gel slots and run electricity through the gel for ‘x’ time. The larger the fragment, the less it’ll move down the gel. The smaller the fragment the more it will move. So you should see the 2600 basepair move a teeny bit more than the 2700 base pair dna [more...]
Date: 2010-10-06 12:01:00
DNA 2050 One Year Anniversary Event « Chicago Travel Blog ...
DNA 2050 is a store that will never go out of style. Why? It specializes in denim and even if your taste in fashion includes marabou feathers, everyone owns a pair of jeans. DNA 2050 opened in The 900 Shops one year ago and they’re celebrating with a weekend full of of festivities! From October 15-17, receive 15 percent off all items (even non-denim apparel and accessories), as well as an additional gift with any denim purchase. View the latest in fall fashion at one of the trunk shows taking place throughout the weekend and be sure to try your luck at the raffle giveaways. Oh, and did we [more...]
Date: 2010-10-10 19:54:07
To obtain these structures, a new, efficient solid-phase synthesis protocol was developed as well. To determine the binding affinity and selectivity of these compounds among all possible 4-base pair sequences of DNA, a high throughput (HT), fluorescence intercalator displacement (FID) assay was employed. The FID results indicate that these compounds are good DNA minor groove binders as predicted (more than 50% fluorescence decrease at 10 μM for A/T only sequences); the amidine group improves the binding affinity and selectivity compared to amino acid-benzimidazole conjugates without an [more...]
Date: 2010-08-25 22:40:17
DNA methylation analysis of multiple tissues from newborn twins ...
DNA methylation analysis of multiple tissues from newborn twins reveals both genetic and intrauterine components to variation in the human neonatal epigenome Miina Ollikainen1,3, Katherine R. Smith2, Eric Ji-Hoon Joo1,3, Hong Kiat Ng1,3, Roberta Andronikos1,3, Boris Novakovic1,3, Nur Khairunnisa Abdul Aziz1,3, John B. Carlin2,3, Ruth Morley1,3, Richard Saffery1,3,*† and Jeffrey M. Craig1,3,† 1Developmental Epigenetics and 2Clinical Epidemiology and Biostatistics Unit, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria 3052, Australia [more...]
Date: 2010-08-24 16:08:26
Male Gender Identity in an Individual With Complete Androgen ...
I remember that guy drawing DNA double-bonds, DNA base pairs, A, T, C, G, and droning on about how the DNA analysis was done and how DNA works and what DNA is made of and blah blah blah… He was so boring and so uninspiring and didn't [more...]
Date: 2010-06-27 20:20:48
Trinity - Biology (Trent Uni Trip)
The DNA runs through an agarose gel matrix due to a current and the smaller the piece (the fewer base pairs) the further the DNA will go. The DNA can then be analysed; for example DNA from a crime scene against suspects or finding faulty genes for diagnosis of disorders like cystic [more...]
Date: 2010-10-08 00:00:00
Couple Shocked To Find Out They Are Half Siblings | The Frisky
The pair, who have a young son, recently confirmed through DNA testing that they are each other’s half-siblings. Understandably, the couple has chosen to remain anonymous and go by fake names in the press, fearing how public knowledge of the news would affect their son. It was discovered that things were a little too all in the family for James and his girlfriend Maura when James reconnected with his estranged mother and started telling her about his long-term girlfriend. Once the mother learned the full name of Maura’s father, Tom, she confessed to her son that the dad he was raised [more...]
Date: 2010-06-01 18:56:29
DNA sequencing » Blog Archive » DNA Sequencing Methods
DNA Sequencing – How DNA Sequencing Works – Methods for … An explanation of how DNA sequencing works. … Typically, the automated sequencing method is only accurate for sequences up to a maximum of about 700-800 base-pairs in length. … DNA Sequencing Simulation (Sanger Method) Within each reaction mixture are the following items: a DNA segment of unknown … The other DNA sequencing method (named for Allan Maxam and Walter … Next-Generation DNA Sequencing Methods 921k – Adobe PDF – View as html generation DNA sequencing technologies highlight the striking impact … [more...]
Date: 2010-09-28 04:29:58
The biggest genome EVER « Mr. Barlow's Blog
The human genome is composed of a total of just over 3 billion DNA base pairs, basically 3 billion letters of instructions. Instructions to make a human. Scientists have now found the biggest genome ever! A rare Japanese flower named Paris japonica sports an astonishing 149 billion base pairs, making it about 50 times the size of a human genome—and the largest genome ever found. Until now, the biggest genome belonged to the marbled lungfish with 130 billion base pairs. The genome of the new record-holder would be taller than Big Ben if stretched out end to end. Amazingly it fits inside [more...]
Date: 2010-10-09 23:00:22
[DNA: Replication, Repair, and Recombination] Inefficient ...
Inefficient Proofreading and Biased Error Rates during Inaccurate DNA Synthesis by a Mutant Derivative of Saccharomyces cerevisiae DNA Polymerase δ* Stephanie A. Nick McElhinny ‡ , Carrie M. Stith § , Peter M. J. Burgers § and Thomas A. Kunkel ‡ ‡Laboratory of Molecular Genetics and Laboratory of Structural Biology, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709 and the §Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110 1 To whom [more...]
Date: 2007-01-19 08:00:00
GENE DESIGN PROGRAM FROM JOHNS HOPKINS SIMPLIFIES, AUTOMATES AND ...
GeneDesign automates the process of determining which base pairs -- the building blocks of DNA -- should be linked together in a particular order to make a gene, according to Jef Boeke, Ph.D., professor of molecular biology and genetics and ... the tasks required to design and manipulate synthetic DNA sequences. The program allows the user to start with either the sequence of the amino acid making up the protein or the bases making up the gene that codes for that protein. [more...]
Date: 2006-02-15 08:00:00
How can we get cancer if DNA Polymerase corrects errors.?
If our DNA Polymerase corrects all the errors in base pairing in our DNA than how do we get cancer? If im not wrong we get cancer because the cell builds the wrong protein due to wrong nitrogenous bases. Thanks! [more...]
Date: 2010-09-18 03:49:19
Critiquing LaPlant et al, 2010, in Nature Neuroscience, Part 1 ...
What is blown up at the bottom is one of those specific base pairs, cysteine. What DNA methylation does is put a methyl group (highlighted in yellow) onto the cysteine. It doesn't look like much, but this methyl group is very important. [more...]
Date: 2010-10-13 10:54:28
Mechanistic Studies with DNA Polymerases Reveal Complex Outcomes ...
Biochemical analysis of DNA polymerase activity has shown that these enzymes utilize a series of molecular checkpoints that typically promote formation of “Watson-Crick” base pairing geometry and, therefore, stable propagation of DNA [more...]
Date: 2010-08-19 07:00:00
Using a Home DNA Test Kit
A home DNA test kit is exclusively used in the sampling stage of a home based DNA paternity test. The key component in the kit is usually a pair of serrated cotton swabs, and some DNA free sample tubes. These two components allow the [more...]
Date: 2010-10-11 20:41:48
When will the average price for commercially available DNA ...
Is the price of DNA synthesis going the way of Moore's Law? Recent prices from top synthesis companies (average price per base pair): GeneArt: $0.53 DNA 2.0: $0.69 Codon Devices: $0.79 Mr. Gene: $0.39. See http://www.1centbp.com/ for [more...]
Date: 2010-09-16 18:40:00
DNA Transcription (Advanced)
Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT The Central Dogma of Molecular Biology: “DNA makes RNA makes protein” Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place… inserting it with [more...]
Date: 2010-10-03 03:02:09
How would you use agarose gel electrophoresis of DNA to detect ...
well you would need a template DNA to compare it to…also some digestion enzyme to cleave it at known base pairs. you would introduce the enzyme to both the template DNA and the one you want to compare to it. digest them. run them in the gel and you’ll see bands at different bp markers for each column. Know better? Leave your own answer in the [more...]
Date: 2010-10-12 12:03:47
Claim White flower has world and 39s longest genome plasmid dna
The size of an organism's genome is typically measured by the number of bases it contains — base pairs being the building blocks of DNA. The human genome [more...]
Date: 2010-10-07 22:46:50
LabCorp is the First National Laboratory to Offer - DNA Paternity ...
LabCorp's New Paternity Test Uses Promega Kits to Yield 'Double-Blind' Results LabCorp has begun offering a DNA-paternity testing service that uses a pair of kits that analyze 21 genetic markers, and that have “overlapping genetic [more...]
Date: 2010-04-01 06:33:02
DNA Programmed To Make Möbius Structures
The origami method relies on a long, single stranded segment of DNA, used as a structural scaffold and guided through base pairing to assume a desired shape. Short, chemically synthesized “staple strands,” composed of complementary [more...]
Date: 2010-10-04 15:53:17
20000 Genes Under the Brain: Genes->Brain->Man
To assess gene expression in each small bit of tissue, researchers expose its RNA to a gene chip, or DNA microarray. These small devices are coated with clusters of identical DNA molecules, called probes, within separate areas. Each probe binds to the RNA of a specific gene—the one that contains a complementary set of chemical units, or bases. In a DNA molecule, the base adenine (A) sticks to thymine (T), and guanine (G) pairs with cytosine (C). Thus, the sequence that would bind to the strand illustrated above is (from top to bottom): T, C, C, T, G, C, A. In this way, a chip records which [more...]
Date: 2010-09-02 16:33:00
dna: maternity jeans | Human Identification Daily
Does any one else out there have an impossible time finding maternity jeans?? Especially being short– it’s so hard to find pants. I’ve tried on a bunch of pairs, hoping that they had magically improved since my last pregnancy– but … dna: maternity jeans Related posts:DNA Maternity Testing – Home Legal Maternity DNA Test Teen who prefers Genes to Jeans | Ask Kate DNA Maternity Test | [more...]
Date: 2010-10-12 01:59:00
Artificial Life Created in a Laboratory for the First Time ...
They manufactured a new chromosome from artificial DNA in a test tube, then transferred it into an empty cell and watched it multiply – the very definition of being alive. The man-made single cell “creature”, which is a modified version of one of the simplest bacteria on earth, proves that the technology works. Now Dr Venter believes organism, nicknamed Synthia, will pave the way for more complex creatures that can transform environmental waste into clean fuel, vaccinate against disease and soak up pollution. But his development has also triggered debate over the ethics of “playing [more...]
Date: 2010-05-21 13:46:05
can some1 help with Genetics home work? | Mountain Bike Shoes
In humans, blood type inheritance is an example of A. complete dominance. B. codominance. C. incomplete dominance. D. predominance. 5. During division in cellular reproduction, the DNA and cytoplasm of the parent cell is distributed to two A. clones. B. chromosomes. C. daughter cells. D. simplified cells. 6. Suppose you’re looking through a microscope and you see an exchange of chromosome segments between chromosome pair 7 and chromosome pair 15. This condition is an example of A. translocation. B. duplication. C. inversion. D. relocation. 7. A DNA molecule has a unique [more...]
Date: 2010-10-11 06:15:39
Ultra High Throughput Sequencing in Human DNA Variation Detection ...
Home / PLoS ONE, Science/Tech/Health / Ultra High Throughput Sequencing in Human DNA Variation Detection: A Comparative Study on the NDUFA3-PRPF31 Region Ultra High Throughput Sequencing in Human DNA Variation Detection: A Comparative Study on the NDUFA3-PRPF31 Region Background Ultra high throughput sequencing (UHTS) technologies find an important application in targeted resequencing of candidate genes or of genomic intervals from genetic association studies. Despite the extraordinary power of these new methods, they are still rarely used in routine analysis of human genomic variants, in [more...]
Date: 2010-10-01 02:15:57
PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading
Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]
Date: 2009-11-13 08:00:00