Our blogs and videos choice: purification of dna

Healing Dna With Light, Sound And Acupuncture

There are many other ways to enhance the torsion field in our body like sunlight, negatively charged fresh air, clean water, natural medicines, and healthy foods.   Another way to improve and repair DNA on a cellular level is acupuncture. Acupuncture can be used on the pericardium and heart meridians to activate the thymus gland and improve immune function. This can be very useful in treating autoimmune diseases. Increased the energy in these meridians can activate higher frequencies. Higher frequencies lead to lower cellular oxidation rates and less cellular aging. Acupuncture on the Du [more...]

Date: 2010-09-07 12:05:19

Blog posts (21) | Videos (5)
 


JoVE: Electroeluting DNA Fragments (Video Protocol)

Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution from glass or silica particles, DEAE-cellulose membranes, "crush and soak method", electroelution or very often expensive commercial purification kits. Thus, selecting a method will depend mostly of what is available in the laboratory. The [more...]

Date: 2010-09-05 22:00:00


Berkeley Lab News Center Press Release: New Electrostatic-based ...

In a typical experiment, a microarray is prepared and mounted in a well chamber and the DNA is hybridized (a standard technique in which complementary single strands of DNA bind to form double-stranded DNA "hybrids"). A suspension of negatively-charged silica microspheres is then dispersed through gravitational sedimentation over the microarray surface, a process which takes about 20 minutes. Because the substrate or background surface of the microassay is positively charged, the silica microspheres will spread across the entire surface and adhere to it. However, on surface areas containing [more...]

Date: 2008-06-30 16:00:00


Lab Rat: DNA Extraction

Precipitate the DNA by adding isopropanol. Isopropanol is another old favourite, it just clumps DNA, making very very hard-to-see pellets. You've probably guessed by now but the next stage is: centrifuging, to collect the pellet. 14. Wash pellet with ethanol. Ethanol removes any residual salt as there will be some magnesium and various others in the DNA (DNA has an overall negative charge due to the phosphate backbone which collects positively charged salts). Washing involves adding ethanol, shaking very gently and then (surprise surprise) centrifuging to pellet the DNA again and remove all [more...]

Date: 2008-08-12 09:21:00


Strawberry DNA Extraction | Serendip's Exchange

DNA Isolation using Strawberries (wear eye protection, or be careful).  Working in groups (for supervision), but each individual will do their own. Place 1 strawberry in the plastic bag.  Remove air and seal. Mash strawberry for 2 minutes. Add ~10 ml DNA Extraction buffer (Pre-measured).  Remove air and seal. Mash for 1 minute. Pour solution through coffee filter  into paper cup Pour ~2 ml of this solution back into the 15 ml tube.  It will be very red. Pour ~ 4 ml (2X the volume of strawberry solution) of cold ethanol (found on freeze door in 50 ml tubes) so that it lays on top of [more...]

Date: 2008-07-30 07:00:00


Antibody Purification Using Membrane Adsorbers - Results from a ...

Dec 1, 2008 By: Leonardo Giovannoni, PhD, Marco Ventani, Uwe Gottschalk, PhD BioPharm International Volume 21, Issue 12 ABSTRACT Most antibody manufacturers currently use a three-column platform comprising Protein A affinity chromatography for product capture, followed by anion exchange (AEX) chromatography in flow-through mode to extract negatively charged contaminants, and then cation exchange (CEX) chromatography or hydrophobic interaction chromatography (HIC) in retention mode to remove positively charged contaminant species. This article presents a new process that uses membrane [more...]

Date: 2008-12-01 05:00:00


What do the bands in a DNA gel contain?

sweetgal2301 |  Thursday, 23 September 2010 at 8:18 pm They contain DNA. As a gel, an agarose medium is porous, which means … we see the agarose as a lump of gel .. in fact it is made up of clump of gel like balls attached to each other. so in between these balls there are spaces. So these bases can be used by certain molecules to move around. So when you give an electric current to negative or positive charged molecules … they can move through these pores to their opposite attracting charge. the DNA product is usually negatively charged so they will migrate from the positive end [more...]

Date: 2010-09-23 19:55:35


Why Do Histones Bind Tightly To DNA? | Why

Histones are an important part that helps bind the DNA chromosomes together. They are amino acids that are composed of protein and are positively charged. Since the acidic part of the DNA is negative and the Histones are positively charged, it helps bind the DNA together. Protein interactions play an important role in the functions of the DNA. The five types of Histones are H1 (H5), H2A, H2B, H3, H4 and they are consistently present in all forms of DNA. DNA (Deoxyribo Nucleic Acid) is the genes that a living organism, cell, virus inherits and sometimes referred to as a “blueprint” (basic [more...]

Date: 2010-09-21 17:56:17


DNA Isolation and Modeling « NWABR

This entertaining and inexpensive protocol was adapted from Biotechnology in the Classroom, UC Davis and Diane Sweeney, Biology — Exploring Life. First we, the teacher-students line up for our materials: Dr. Hutchison regales us as we select strawberries and collect materials: This flexible protocol has been used with first graders all the way through high school and administrators. You can make it as in depth as you like. All materials can be bought in a grocery store, there are no expensive reagents. This was my lovely strawberry, in a sealed plastic bag and on a paper towel, [more...]

Date: 2010-03-07 00:00:57


Part I – THE ANTI-GRAVITATION EFFECT IN OUR EVERYDAY LIVING

Following the electrical charge (+), and the direction of the vector toward the right → this positive charge used as a unit of measurement is associated to a DIMENSION. Now as Karisma uses the negative charge (-) and the direction of the vector toward the left in the equation 0=2-0-0-2 ← this negative charge used as a unit of measurement is related to the anti-dimension (is as a mirror, an illusory image). In these equations the VECTOR symbolizes the direction in the FLOW OF LIGHT/ENERGY. Also in these equations when the dense charge (+) is in equilibrium with the negative charge, [more...]

Date: 2010-10-08 03:10:25


Why Do Hydrogen Bonds Form?

It is the interaction between hydrogen and an electronegative atom which has a partial negative charge. In order to create a bond, the hydrogen having the positive charge, must be covalently bonded to another electronegative atom which has the negative charge. These electronegative atoms that form a bond between hydrogen atoms are called hydrogen-bond donors. This atom can either be oxygen, nitrogen or fluorine. Whether bonded to a hydrogen atom or not, the electronegative atom of these elements is a hydrogen bond acceptor. Example of this is the ethanol, a bond between hydrogen and oxygen. [more...]

Date: 2010-10-03 16:52:58


PLANT TISSUE CULTURE TECHNIQUE AND PCR TECHNOLOGY

SSR assays require a minimal amount of genomic DNA. Most of the crop improvement programs are confined to evaluation and selection of naturally occurring clonal variations. In vitro culture techniques provide an alternative means of plant propagation and an important tool for crop improvement. The in vitro cultures are also of low risk for genetic variation since it is more resistant to genetic changes while occurrence of cell division in vitro condition. Gel electrophoresis is an important molecular biology tool which enables us to study DNA. It can be used to determine the sequence of [more...]

Date: 2010-09-08 04:07:38


DNA fingerprinting simplified

One of the most frequently used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic technique often used in genetic or DNA fingerprinting. The procedure is achieved by moving negatively charged nucleic acid molecules through a gelatinous substance known as agarose by using an electric field. Andrew, in collaboration with his father and other researchers, completed the study, which is published in the January 2008 issue the American Society of Horticultural Science’s journal HortTechnology. The youngest author ever to publish in an ASHS journal, Andrew [more...]

Date: 2008-05-14 04:52:12


Imagine Day Activity: Extract Your Own DNA! | iGEM of UBC

This is important because we are trying to gather a large amount of DNA in one place.  If they are negatively charged they will repel each other.  If the negative charge is matched by a positive charge (the Sodium ions) it will make it much easier for the DNA to gather together. Step 5: Fill the rest of the shot glass of alcohol -You must be VERY careful in this step to pour the alcohol on TOP of the spit and not let it mix.  You can do this by pouring it down the side of the glass very gently and slowly.  Note: you must have steady hands, so no drinking the alcohol beforehand. Why are we [more...]

Date: 2010-09-06 23:53:48


Dr. Mona Harrison MD. “Sacred Water” Pt1

Especially the brain and liver have water that has a large number of negatively charged hydrogen ions. A water cluster is a ring of six molecules that share common hydrogen bonds. The six-sided shape enters the cell most easily, like a master key that opens the locks on many doors. In its free state in nature, the water molecule has two positively charged hydrogen ions and one negatively charged oxygen ion. Cellular processes in plants and animals store energy in the hydrogen ions by capturing free electrons. The stored electrons change the hydrogen ions from a positive charge to a negative [more...]

Date: 2010-10-05 22:52:42


Why the high energy bond in ATP isn't very high « Chemiotics II

Lotsa stuff, basically scientific — molecular biology, organic chemistry, medicine (neurology), math — and music Wavefunction had an interesting post about ATP and bond energies.  http://wavefunction.fieldofscience.com/2010/09/does-bond-cleavage-require-or-release.html.  Like most of his posts, it got me to thinking, particularly about a lot of the blarney written about ATP.  I was taught that ATP contains a ‘high energy’  phosphate bond.  This really isn’t correct.  ATP is used by the cell as ‘money’.  Some activities such as breaking down glucose  produce ATP [more...]

Date: 2010-09-27 01:05:13


what properties of DNA allow it to be analyzed using gel ...

Question by b3th: what properties of DNA allow it to be analyzed using gel electrophoresis? Best answer: Answer by dpfw16 The main property would be it’s negative charge and, secondarily, it’s size. The DNA is positively charged, so during gel electrophoresis, you apply a current and the DNA moves toward the negative electrode (opposites attract). Also, as DNA moves, smaller pieces move faster than larger pieces, so you get spreading out (this is assuming the DNA is cut up- if it’s whole, then the entire piece will run as one piece). So, that’s why I answered negative charge and, [more...]

Date: 2010-10-01 03:59:26


Biosensors: Morpholino offers you more

Molecular biologists and geneticists often use DNA microarrays to detect DNA, measure gene expression levels and detect the single-nucleotide polymorphisms that are responsible for various genetic diseases. A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the [more...]

Date: 2010-10-13 09:52:22


Biosensors: Morpholino offers you more

A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the ability to withstand wear and tear over many cycles). Fig. 1: Scanning electron microscopy image (left) of a cluster of parallel SiNWs, and a schematic illustration (right) of the Morpholino-based sensing [more...]

Date: 2010-10-13 12:52:44


In the gel electrophoresis chamber the DNA fragments migrate ...

Question by J J: In the gel electrophoresis chamber the DNA fragments migrate toward the _____ charged pole? A) positively B) negatively C) positively or negatively charged pole, depending on the charge of the fragment D) enzymatically E) radioactively I think C but wanted to make sure Best answer: Answer by Scientist VK DNA is slightly negative due to the phosphate group on the nucleotide so the fragments are always attracted to the positive pole The answer is A What do you think? Answer [more...]

Date: 2010-09-29 19:54:53


Electrostatic trap catches tiny particles - physicsworld.com

Since their invention about 40 years ago this technique has been used with great success in biophysics, helping researchers to unravel the complex elasticity and folding dynamics of DNA, for instance. But, because optical tweezers struggle to hold on to objects that are significantly smaller than the wavelength of light, they cease to work for objects that are smaller than 100 nm. Charge rather than size Now, a group at ETH Zurich has developed an alternative mechanism for trapping particles that does not suffer from the same limitation. The device works by suspending particles within an [more...]

Date: 2010-10-06 17:00:00



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