Our blogs and videos choice: dna

Plasmid double locus sequence typing for IncHI2 plasmids, a ...

Plasmid double locus sequence typing for IncHI2 plasmids, a subtyping scheme for the characterization of IncHI2 plasmids carrying extended-spectrum β-lactamase and quinolone resistance genes Aurora García-Fernández and Alessandra Carattoli* Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy *Corresponding author. Tel: +39-06-49903128; Fax: +39-06-49387112; E-mail: alecara{at}iss.it Received October 9, 2009. Revision requested January 25, 2010. Revision received February 26, 2010. Accepted March 4, 2010. Abstract Objectives IncHI2 [more...]

Date: 2010-03-31 07:00:00

Blog posts (34) | Videos (6)
 


Sandwalk: Nobel Laureate: Walter Gilbert

The Nobel Prize in Chemistry 1980. "for their contributions concerning the determination of base sequences in nucleic acids" Walter Gilbert (1932 - ) was awarded the Nobel Prize for developing a chemical method of sequencing DNA (with Allan Maxam). The method relied on chemical reactions that cleaved DNA at specific residues. By carrying out partial reactions where only one cleavage occurred in each DNA strand, it was possible to separate the cleavage products on an acrylamide gel and determine the position of each residue by the length of the fragment. The chemical sequencing strategy has [more...]

Date: 2008-12-10 14:35:00


Basics: Sequencing DNA, Part 1 « Genetic Inference

However, in certain respects Sanger Sequencing is still top-of-the-range. The 4-channel capillary approach lets you sequence DNA pretty fast, and each machine can often do hundreds of reactions at once. It was these machines that first sequenced the human genome. In addition, you can sequence relatively long sequences of DNA; up to 1000 nucleotides (after that, it gets difficult to tell the difference between molecules of different sizes). We have yet to make a machine that can sequence DNA fragments of this length faster than Sanger Sequencing (yet…). The modern Sanger Sequencing machines [more...]

Date: 2009-04-17 21:31:39


Neandertal DNA and Proteins

With the completion of the Neandertal genome by the team of Dr. Svante Paabo and a closer look at their proteins by Gregory Hannon’s team at CSHL, scientists reveal incredible similarities between Neandertals and humans. Neandertals, the extinct species of what are most likely our closest relatives, lived on earth at the same time as our human ancestors but died out about 30,000 years ago. With the sequence of their genome now complete, we can compare the DNA to humans and chimpanzees to learn more about what makes humans unique as a species. The discovery of fossils is an exciting link to [more...]

Date: 2010-05-19 13:58:17


Origin of the human malaria parasite in gorillas - TalkRational

But researchers from the US, three African countries, and Europe have examined malaria parasites in great ape faeces. They found the DNA from western gorilla parasites was the most similar to human parasites. Liu, W. et al. (2010) Origin of the human malaria parasite Plasmodium falciparum in gorillas. Nature, 467, 420-425. Plasmodium falciparum is the most prevalent and lethal of the malaria parasites infecting humans, yet the origin and evolutionary history of this important pathogen remain controversial. Here we develop a single-genome amplification strategy to identify and characterize [more...]

Date: 2010-09-22 17:35:43


Junk DNA and Complexity Science: A Sad mix

A quote from an extremely mediocre book, Complexity: A Guided Tour. Like most people coming out of the 'complexity sciences', the author has a mediocre grasp of molecular biology, both current developments, and the history of the field. It's frustrating that these network people keep hyping supposedly new revolutionary discoveries made the past 10 years of the genome era - discoveries, that in fact have been known for decades. For people clearly new to biology, molecular biology before the year 2000 was a big black hole of ignorance. There are several claims that make me angry in this book, [more...]

Date: 2010-05-21 19:56:10


Scientists say rare plant has biggest genome yet « Xenophilia ...

A genome is the full complement of an organism’s DNA, complex molecules that direct the formation and function of all living organisms. The size of an organism’s genome is typically measured by the number of bases it contains – base pairs being the building blocks of DNA. The human genome, for example, has about 3 million bases and measures about 6 feet in length. The marbled lungfish has a whopping 130 million bases. And the 12-inch (30-centimeter) flower studied by Leitch turns out to have 150 million. Outside experts were impressed. “This is certainly an enormously large genome,” [more...]

Date: 2010-10-08 08:37:15


University Of Pune SYLLABI OF BACHELOR OF PHARMACEUTICAL SCIENCES ...

DNA Hybridization, Blotting techniques, polymerase chain reaction (PCR) Site directed mutagenesis, Restriction Fragment Length Polymorphism (RFLP), Human Gene therapy, DNA fingerprinting. Gene synthesis & gene machine, Gene sequencing [more...]

Date: 2010-09-02 07:32:33


Synthetic Biology: Building Life (Potential & Dangers ...

But today, applications of our understanding of DNA in areas such as genetic engineering and cloning seem primitive compared to synthetic biology, a field in which the basic building blocks of life are pieced together from scratch to suit the needs of research, medicine, and the environment. Synthetic biology combines knowledge of genetics and cell biology with principles of engineering and is limited only by scientists’ imaginations.  But the field is still in its infancy, and initial ventures into figuring out how best to put the basic components of DNA together synthetically have [more...]

Date: 2009-03-10 17:15:19


Watch Splice Full Movie Online | Download Splice movie Full Length ...

They specialize in splicing together DNA from diverse animals to develop fantastical new hybrids. The charismatic couple wants to make use of human DNA in the new hybrid – anything that could yield astronomical healthcare positive [more...]

Date: 2010-10-09 06:30:52


DNA art imitates life: Construction of a nanoscale Mobius strip

The circulations of earth's warmer and cooler ocean currents for example, describe a Möbius shape. Other topological structures are common to biological systems, particularly in the case of DNA, the 3 billion chemical bases of which are packed by the chromosome inside the cell, using topological structures. "In bacteria, plasmid DNA is wound into a supercoil," Yan explains. "Then the enzymes can come in and cut and reconfigure the topology to relieve the torsion in the supercoil so that all the other cellular machinery can have access to the gene for replication, transcription and so [more...]

Date: 2010-10-08 21:50:00


My Helical Tryst: Single Molecule DNA Sequencing

There are a number of companies that have recently launched competing technology platforms that are capable of sequencing DNA at the single molecule level. You may ask, why? Well, the answer is simply cost-effectiveness. DNA sequencing technology has been around for several decades, and to date, the Human Genome Project (HGP) is considered the pinnacle application of that technology. The cost of the HGP was approximately $3 billion and took about a decade and half. It was the molecular biology equivalent of putting a man on the moon. But what is the next step? Well, if we can systematically [more...]

Date: 2009-02-11 22:17:00


The Human Provenance Project « Free Movement

It sounds like the title of a dystopian science fiction film, and it is every bit as bad as it sounds. The first I heard of it was on 14th September 2009 after this letter was circulated to UKBA stakeholders. I had a little rant about it at the time in another forum, complaining there was something unpleasantly eugenicist about it, lamenting the daft liberal arts graduates who approved it who understand neither science nor statistics and suggesting they start measuring skull size and penis length for good measure. Free Movement is a proud graduate in, er, history, incidentally. The Human [more...]

Date: 2009-10-02 14:11:16


THE SOUL! A Personal Reflection On The Reality Of The Human Soul ...

THE SOUL! A Personal Reflection On The Reality Of The Human Soul For All Those Who Long To Rekindle The Sense Of The Great Mystery Of The Human Soul Which Seems To Have Been Lost In The Post-Modern Era. By jamesmessig Hi Folks, I thought that you would enjoy this writing on the great dignity of the human soul.  This article offers a sharp contrast to the main topics on which I tend to post, and was written and compiled from my heart. I have often posted articles on extreme manned star ship technologies, and as a physicist, I will continue to do such. However,  I offer this rather long [more...]

Date: 2010-10-10 06:55:33


My Weblog on Bioinformatics, Genome Science, Next Generation ...

Just saw this paper Sequencing and analysis of an Irish human genome. AFAIK WGS is usually done at 30x coverage. In this paper, the authors “describe a novel method for improving SNP calling accuracy at low genome coverage using haplotype information.” I thought it was pretty good considering that they had 99.3% of the reference genome covered for 10.6x coverage. That leaves only like 21 Mbases missing .. For those interested in the tech details Four single-end and five paired-end DNA libraries were generated and sequenced using a GAII Illumina Genome Analyzer. The read lengths of the [more...]

Date: 2010-10-12 10:49:00


Chemical synthesis of the mouse mitochondrial genome « too New to yoU

However, this study used the basic synthesis unit is a nucleotide containing thousands of DNA (deoxyribonucleic acid) fragments. Because the basic synthesis unit larger, the final synthesis in the verification is correct the genome will bring some trouble. Thus, the research team invented a new method of synthesis of the genome, using the basic synthesis unit is only 60 nucleotides of DNA containing fragments, placing them well-designed environment, you can connect into the whole genome. The group believes that this is by far the easiest way to synthetic genome. In order to verify the [more...]

Date: 2010-10-13 07:19:52


Overcoming Bias : Sex At Dawn Is Right

I like to think of myself as courageously seeking out important truths, however uncomfortable. But like most would-be-courageous folk, I don’t really know what I want until I get it. I was excited to read the contrarian Sex at Dawn, which suggests sexual promiscuity is our forager heritage. But that pretty sparkler was really a grenade – its uncomfortable truths shook me to my core. To hear a different view, immediately afterward I read Evolutionary Biology of Human Female Sexuality , which supports a standard view of foragers as long-term pair-bonders. By comparison, EBoHFS is [more...]

Date: 2010-10-12 20:00:14


The Quest for Height: Grow Taller | Increase Height | Bone Size ...

Previously, we discussed some methods that may govern cellular senescence and in turn growth plate senescence including DNA Methylation and telomere length.  Endochondral ossification stops as a result of cellular senescence in the growth plate.  DNA Methylation and telomere length may regulate senescence in the stem cells but alkaline phosphatase may determine the terminal chondrogenic differentiation in growth plate chondrocytes.  Studying growth plate alkaline phosphatase activity may be a key in curing dwarfism and in increasing height in normal individuals. F-spondin regulates [more...]

Date: 2010-10-09 18:32:00


Cellular Clock Movement Supports Seismic Modifications In ...

Human cells have only got so many lives, same as cats. When divided over time they are altered, slow down and gradually quit separating – this is referred to in scientific terms as cellular senescence – . Already biologists understand that a cellular timer made up of structures at the end of the chromosome called Telomeres, document any activity regarding the cells’ lives and the number that have been exhausted. Until this point, researchers have yet to learn how the running of the clock determines the mortality of the cells. An investigation [more...]

Date: 2010-10-11 21:49:26


Information About Non Paternity And Non Maternity

All the same, requirements for consent and counselling for DNA testing are unique from each country's laws and policies. DNA testing would be the most state-of-the-art and precise technique to figure out both non-paternity and non-maternity. In figuring out if the presumed parents are genuine biological ones or in determining actual biological mother or father-child bond, subjects undergo parental screening. This sort of practice uses the genetic fingerprinting (or DNA profiling), that is a approach to recognize identity by means of DNA profiles. A paternity test serves as an inherited proof [more...]

Date: 2010-10-09 18:01:01


PLoS ONE: Analysis of Short Tandem Repeats by Parallel DNA Threading

Abstract TopThe majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the [more...]

Date: 2009-11-13 08:00:00


My faith is falsifiable, Professor Coyne. Is yours? | Uncommon Descent

The creation of a machine that was capable of conversing at length about any topic – including its own mental states and life story – in such a way that it could fool an audience of intelligent people into thinking that it was human. Observations that would cause me to abandon belief in Intelligent Design 1. An empirical or mathematical demonstration that the probability of the emergence of life on Earth during the past four billion years as a result of purely natural processes, without any intelligent guidance and starting from a random assortment of organic chemicals, is greater than [more...]

Date: 2010-10-13 00:36:41


Restriction Fragment Length Polymorphism

The analysis of a relatively small number (4-6) of highly polymorphic regions or loci within the human genome, commonly referred to as minisatellites or variable number of tandem repeats (VNTRs), is sufficient to generate DNA profiles [more...]

Date: 2010-08-01 06:59:59


Bio-hackers? Biology Is Technology: Robert H. Carlson

DNA for these purposes is synthesized either in the lab where it is to be used or elsewhere in a dedicated facility and delivered by express mail. Mail-order oligonucleotides were used in 2002 to build a functional poliovirus genome from constituent molecules for the first time. Since then constructs of ever-increasing length have been announced, and a full bacterial chromosome was recently synthesized from short oligos.”This capability so far ahead of what I would have thought possible. The author claims that the technology is moving forward in a fashion consistent with the famous [more...]

Date: 2010-10-10 04:54:00


Aniplex Books R.O.D -The Complete- Blu-ray Box

Originally created as a series of light novels and manga volumes, R.O.D READ OR DIE was brought to animated life by the triumvirate of Creator/Screenwriter Hideyuki Kurata, Director Koji Masunari and Animation Character Designer Masashi Ishihama. The trio has since worked together on the anime series Kamichu! (with R.O.D Original Character Designer Taraku Uon) and the recent full-length animated movie Welcome to THE SPACE SHOW (with R.O.D. -THE TV- producer and ―Besame Mucho‖ collaborator Tomonori Ochikoshi), however, their seminal R.O.D creation remains their most beloved and best-known [more...]

Date: 2010-10-12 10:07:00


Mutation Rate In Humans Gets Measured By Direct Sequencing

With better measurements of mutation rates, we could improve the calibration of the evolutionary clock, or test ways to reduce mutations, for example. Even with the latest DNA sequencing technology, the researchers had to design a special strategy to search for the vanishingly rare mutations. They used next-generation sequencing to establish the order of letters on the two Y chromosomes and then compared these to the Y chromosome reference sequence. Having identified 23 candidate SNPs – or single letter changes in the DNA – they amplified the regions containing these candidates and [more...]

Date: 2009-08-27 14:01:30


Evidence of altered DNA integrity in the brain regions of suicidal ...

Deoxyribonucleic acid (DNA) integrity plays a significant role in cell function. There are limited studies with regard to the role of DNA damage in bipolar affective disorder (BP). In the present study, we have assessed DNA integrity, conformation, and stability in the brain region of bipolar depression (BD) patients (n=10) compared to age-matched controls (n=8). Genomic DNA was isolated from 10 postmortem BD patients' brain regions (frontal cortex, Pons, medulla, thalamus, cerebellum, hypothalamus, Parietal, temporal, occipital lobe, and hippocampus) and from the age-matched control [more...]

Date: 2010-10-09 21:50:38


How Do I separate 2 DNA fragment of similar length on an ...

Question by MrBurlish: How Do I separate 2 DNA fragment of similar length on an electrophoresis gel? One is 2600 base pairs and one is 2700 base pairs Best answer: Answer by Suzy Since you have the DNA fragments already cut that means you’ve already used an enzyme to do so. After that, you use the micropipet to insert the DNA into the gel slots and run electricity through the gel for ‘x’ time. The larger the fragment, the less it’ll move down the gel. The smaller the fragment the more it will move. So you should see the 2600 basepair move a teeny bit more than the 2700 base pair dna [more...]

Date: 2010-10-06 12:01:00


Ancient Aliens 2010: The Visitors pt 2/10 - Get X News

History Channel Documentary (2009)- www.youtube.com UFO Files: Ancient Aliens- www.youtube.com Human’s Extraterrestrial Origins- www.youtube.com Scientists Find Extraterrestrial Genes in Human DNA- www.agoracosmopolitan.com Scientists Confirm Extraterrestrial Genes in Human DNA- www.agoracosmopolitan.com Legacy of the Black Gods – In Time before time (Coming forth from The Akashic Records) Author: Paul Simons (Nebu Ka Ma’at) The Genealogy of Mankind from Ganawah, to Lemuria, to Atlantis, to Egypt and today- www.tamarehouse.com [more...]

Date: 2010-10-10 09:01:04


MCB Exam II / Flashcards - Create Free Flashcards

The complete set of information in an [more...]

Date: 2010-10-11 07:17:28


The Returning Casualty Blog » We thank our partners in DNA analysis

Many professionals have helped TRC in its mission, and the DNA analysts have been among the most supportive. Some of the best in the nation have come forth to assist. TRC’s first door opened at Family Tree DNA, a Houston based service that uses state of the art DNA technology to discover the ancestry of its clients (See the website www.familytreedna.com for interesting information about DNA technology, including an interview with BBC.). The company’s President and CEO, Bennett Greenspan, promptly replied to TRC’s request for assistance. Mr. Greenspan and TRC’s General Counsel, Wesley [more...]

Date: 2010-07-14 06:42:23


Wildlife crime case solved with the help of RZSS scientists ...

Wildlife forensic scientists were able to analyse fragments of the horn and they were not only able to use the DNA from the horn to identify which species of animal the horn belonged to but also the individual zoo animal from which the horn had been taken. This helped investigators to solve the mystery the horn’s origin. The horn came from a white rhino like this one. A white rhino's front horn can grow to be over a metre in length. Credit:RZSS The DNA from the horn was matched to a blood sample take from an African white rhino called ‘Simba’ who had been housed at Colchester [more...]

Date: 2010-10-13 15:17:08


Witness The World's First Double Helix Bridge In Sunny Singapore

Designed to resemble the helicoidal structure of human DNA, the structure has been built almost entirely out of corrosion-free stainless steel that also features state-of-the-art computer controlled mood lighting once dusk sets in. Built according to the vision of the Australian company Cox Group, Singapore’s own Architects 61 and engineering outfit Arup the structure also features nearly 2,250 metre long steel tubes winding in opposing directions. The connection with DNA is also denoted from the two pairs of letters that appear in green and red on the bridge facade once the sun goes down. [more...]

Date: 2010-10-11 09:36:54


Forensic DNA was first used to convict criminals in 1985. Is this ...

Question by Morgan: Forensic DNA was first used to convict criminals in 1985. Is this the same thing as gel electrophoresis? I take AP bio but I obviously forget. Best answer: Answer by CryBabiesShouldntCry A short history from http://www.dna.gov/basics/analysishistory ‘DNA fingerprinting’ or DNA typing (profiling) as it is now known, was first described in 1985 by an English geneticist named Alec Jeffreys. Dr. Jeffreys found that certain regions of DNA contained DNA sequences that were repeated over and over again next to each other. He also discovered that the number of repeated [more...]

Date: 2010-10-03 04:23:14



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