Our blogs and videos choice: pcr dna test

ERV's latest blog on the FDA paper. One step too far?.

PNAS paper, using standard PCR to ID 'positives' and 'negatives', with insane numbers of bands as a result of non-specific primer binding. Insane numbers. As if this is 1990 and we dont know what 'Real Time PCR' and 'Taqman Probes' are. Im going to ignore that and grant the premise they really have IDed viral sequences. And they arent XMRV. They dont cluster anywhere near XMRV on a phylogenetic tree. Theyre something else entirely. Okay, well, logically it could be contamination from mouse DNA. So they checked for that... by looking for mouse mitochondrial DNA. *blink* I understand [more...]

Date: 2010-08-23 07:00:00

Blog posts (6) | Videos (19)
 


HTB – Early infant diagnosis

She concluded that promising POC assays for EID today include: IQuum’s LIAT, SAMBA, CIGHT p24 and possibly CLONDIG’s viral load assay. comment This was an incredibly useful overview and it looks like we can be optimistic about having a POC test for EID in the next couple of years. Other presentations at the conference dealt with the challenges of access to EID. In the same session, Shaffiq Essajee noted that EID access has improved and in some countries more than 50% of exposed infants are tested. But infant testing is usually linked to PMTCT and if coverage is low, so is infant [more...]

Date: 2010-09-30 06:04:59


Blood test detects HIV/AIDS in infants aged 6 months in India DNA ...

Unlike other tests, the HIV DNA PCR test does not rely on the presence of antigens or antibodies in the blood for diagnosis. Instead, it identifies genetic material by highlighting sequences of the virus within the subject’s DNA. All babies born to mothers with HIV are born with HIV antibodies. The currently available tests can predict the chances of HIV infection in infants borne to infected mothers only after the completion of 18 months. This delay in diagnosis often caused a problem for doctors because they found starting the treatment using the life-saving Anti-Retroviral Treatment [more...]

Date: 2010-07-19 22:55:54


Variants of PCR (1)

The bacteriophage T4 DNA polymerase was also tested shortly after the first reports of PCR. It has a higher fidelity of replication than the Klenow fragment. Since it is also destroyed by heat, it has seen little use since the development of thermostable polymerases. The DNA polymerase from Thermus aquaticus (or Taq), was the first thermostable polymerase used in PCR, and is still the one most commonly used. The enzyme can be isolated from its 'native' bacterial source, or from a cloned gene expressed in E. coli. The Stoffel fragment is produced from a truncated gene for Taq polymerase, [more...]

Date: 2008-09-11 03:56:00


- “Paternity Testing”

Paternity testing results? - Yahoo! UK & Ireland Answers Does anybody know how reliable dna testing was in 1982?, could it have … Seriously i think you would be better to ask a Medical Doctor, rather then random ppl … uk.answers.yahoo.com/question/index?qid… Advanced Testing Center - 10 Commercial St , Foxboro, MA 02035 … … igm test pcr test hiv rna test igg testing igm testing pcr testing dna test dna testing confidential herpes testing paternity test paternity testing. … www.getfave.com/12341786-advanced-testing-center court- ordered paternity testing in michigan? how would [more...]

Date: 2010-04-29 23:36:17


FIRST HIV PATIENT NOT DETECTED BY HIV DNA PCR ANTIGEN TEST

FIRST HIV PATIENT NOT DETECTED BY HIV DNA PCR ANTIGEN TEST HIV Patients who were earlier confirmed positive for HIV either through DNA PCR test or Western blot Antibody test after undergoing treatment with HOO-IMM PLUS from HOOTONE [more...]

Date: 2010-09-04 07:36:45



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