Our blogs and videos choice: dna

In Vivo Assembly of DNA Constructs-Columbia Technology Ventures

Eliminates the use of restriction enzymes and many PCR amplification steps by employing the use of double stranded DNA breaks to trigger in vivo homologous recombination • Equally applicable to small as well as large DNA fragment assembly • The cycling of only two selectable markers and the use orthogonal endonucleases allows for repeated rounds of assembly of many DNA fragments • The assembled DNA is in a known location of the yeast genome and can thus be easily transferred from one strain to another Patent Status: PCT/US10/32962 Licensing Status: Available for Licensing and Sponsored [more...]

Date: 2010-10-02 16:40:33

Blog posts (45) | Videos (20)

Plasmid DNA Recovery Using Size-Exclusion and Perfusion ...

Finally, a second size-exclusion chromatography step was carried out to purify the plasmid DNA from other small molecular-weight contaminants. Analytical methods proved that the purified plasmid DNA had a purity of 95% after Sephacryl S1000. Plasmid identity was confirmed by restriction enzyme digestion. Biological activity of the purified plasmid was confirmed in vivo; immunized mice developed a positive antibody response against all HCV structural antigens. This procedure offers an alternative to traditional methods that use organic reagents, mutagenic and toxic compounds, and [more...]

Date: 2008-09-01 04:00:00

A non-radioactive, PFGE-based assay for low levels of DNA double ...

A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells This digital document is a journal article from DNA Repair, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: A PFGE method was adapted to measure DNA double-strand breaks (DSBs) in mammalian [more...]

Date: 2010-09-10 23:55:39

DNA repair causes damage - The Scientist - Magazine of the Life ...

A supposedly accurate DNA repair mechanism employed by cells to fix double-strand breaks can surprisingly increase the nearby mutation rate by up to 1400 times, providing a possible explanation for the accumulation of tumor-causing mutations in cancerous tissues. Previously, "we saw that DNA breaks are present in precancerous legions," said molecular oncologist Thanos Halazonetis of the University of Geneva, who did not participate in the research. "These breaks, based on this finding now, could perhaps explain mutations in oncogenes that drive cancer progression." Cancer [more...]

Date: 2007-08-27 07:00:00

Quick Nucleic Acid Facts

Anne Marie's Chemistry BlogBy Anne Marie Helmenstine, Ph.D., Chemistry Guide | My Bio | RSSMy BioMy BlogMy ForumRSS If you're taking general chemistry, organic, or biochemistry, you'll need to understand some basic concepts about nucleic acids, the polymers used to code the genetic information of organisms. Here are some quick nucleic acid facts to get you started: Nucleic acids are the molecules that code the genetic information of organisms. The two nucleic acids used in repair, reproduction and protein synthesis are deoxyribonucleic acid (DNA, shown) and ribonucleic acid [more...]

Date: 2010-09-27 17:16:46

what is used to splice or cut DNA during the gel electrophoresis??

Question by nefi the dj: what is used to splice or cut DNA during the gel electrophoresis?? please use words an 8th grade student could understand!!!!!!!!!!!!!!!!!!!!!!!!!!! IM NO SCIENTIST!!!!! OK??? Best answer: Answer by chad5871 Proteins called restriction enzymes (or restriction endonucleases). They cut double-stranded DNA (which describes many species’, including human, DNA). Know better? Leave your own answer in the [more...]

Date: 2010-10-03 20:04:34

DNA art imitates life: Construction of a nanoscale Mobius strip

The circulations of earth's warmer and cooler ocean currents for example, describe a Möbius shape. Other topological structures are common to biological systems, particularly in the case of DNA, the 3 billion chemical bases of which are packed by the chromosome inside the cell, using topological structures. "In bacteria, plasmid DNA is wound into a supercoil," Yan explains. "Then the enzymes can come in and cut and reconfigure the topology to relieve the torsion in the supercoil so that all the other cellular machinery can have access to the gene for replication, transcription and so [more...]

Date: 2010-10-08 21:50:00

Slowing the translocation of double-stranded DNA using a nanopore ...

It is now possible to slow and trap a single molecule of double-stranded DNA (dsDNA), by stretching it using a nanopore, smaller in diameter than the double helix, in a solid-state membrane. By applying an electric force larger than the threshold for stretching, dsDNA can be impelled through the pore. Once a current blockade associated with a translocating molecule is detected, the electric field in the pore is switched in an interval less than the translocation time to a value below the threshold for stretching. According to molecular dynamics (MD) simulations, this leaves the dsDNA [more...]

Date: 2010-09-02 17:18:59

.ada.aimi.kisah: dna replication.ape tu?

in this chapter, there's three processes to synthesis protein from DNA. replication(DNA>DNA)> Transcription(DNA>RNA) > Translation(RNA>Protein) just focus on Eukaryotes jea tau. prokaryotes tak masuk dalam syllabus. :) replication of DNA - separation of double strands of DNA is by Helicase. and to prevent the rewinding of the strands, there is SSBp.(Single strand Binding protein) to help. - Leading strand (5' to 3') is copied by DNA Polymerase III which functioned as template for the replication. - lagging strand ( 5' to 3') is produced by DNA Polymerase III and form Okazaki Fragment. [more...]

Date: 2010-08-18 19:04:00

Case 40-2009 — NEJM

A rapid test of a specimen from a buccal swab was negative for influenza A and B antigens, and no parasites were seen on a peripheral-blood smear; other test results are shown in Table 1Table 1 Laboratory Data. .... On the third day, a test for antinuclear antibodies was positive at a dilution of 1:40, in a speckled pattern, and negative at dilutions of 1:80 and 1:160 (reference range, negative at 1:40 and 1:160); a test for antibodies to double-stranded DNA was negative; [more...]

Date: 2009-12-23 21:55:59

DNA replication

The leading strand is the template strand of the DNA double helix so that the replication fork moves along it in the 3' to 5' direction. This allows the new strand synthesized complementary to it to be synthesized 5' to 3' in the same [more...]

Date: 2010-06-28 03:08:46

double stranded dna test - HealthBoards Message Boards

What is a normal ANA test? I read like 1:40. Mine was 1:1280! I'm told it was also speckeled. I had blood drawn for a double-stranded DNA. How long does that take and what is the normal reading for that? Thanks, [more...]

Date: 2009-03-20 17:18:01

my ANA test shows positive,also low levels of C4 & C3.what does it ...

also high TSH level(low T3 & T4 levels),anemia,Rf negative,CRP nagative,Anti ds/DNA negative.I’m too fatigue;having joint pain(rarely),cold extremities,numbness,CNS problems… Tags: amp, anemia, cold extremities, crp, dna, fatigue, high tsh level, joint pain, numbness, rf Posted in test One Response to “my ANA test shows positive,also low levels of C4 & C3.what does it mean?” A positive ANA with a negative double stranded DNA means you have some sort of connective tissue disorder but they have ruled out Lupus. The fatigue is from the anemia, and the cold extremities and numbness are [more...]

Date: 2010-05-20 23:21:14

Vector Art | Free Clip Art | Cancer Icons, Ribbon, Double Helix ...

The vector package contains 4 eps illustrator vectors of cancer icons, which include: no smoking sign, cancer ribbon, dna strand, and nucleus graphic. These vectors are usable to anyone running adobe illustrator 8 or higher. I would encourage all you good hearted designers to donate or volunteer online or at a local cancer related care center or hospital- to show the world that designers do care. For more great graphic design vector resources please check out our sponsor’s ads above. Enjoy. Click here to download Cancer Vectors Free 10 Responses to “Free Illustrator Vectors- April is [more...]

Date: 2008-04-10 04:05:17

Syyn Labs' Glowing DNA - Hack a Day

This beautiful music syncronized double helix was made by Syyn labs. Last time we saw them, they had created that amazing rube goldberg style music video for OK Go. This 100 foot long LED DNA strand took over 1000 combined man hours to build. It took 512 LEDs, 32 LED controllers, 4 Arduinos, 4 computers, over a mile of wire and a very dedicated team, which included . It takes input from beatmatching/VU software as well as a 32 button console or an iPad. You can catch a video of it in action after the break, and they plan on releasing a timelapse of the build in the near [more...]

Date: 2010-10-06 14:29:27


The rate of DNA synthesis E. The series Related posts: DNA REPLICATION (VERY REALISTIC 3D ANIMATION) DNA riposte is the routine of duplicating the double-stranded... HOW DOES EUKARYOTIC DNA REPLICATION DIFFER FROM PROKARYOTIC [more...]

Date: 2010-06-09 07:16:14

[转载]Assay Design and Handling Primers (for Users of QuantiFast ...

If designing your own primers, please follow the guidelines provided in table "General guidelines for design of primers". Since fluorescence from SYBR Green I increases strongly upon binding of the dye to any double-stranded DNA, [more...]

Date: 2010-05-19 05:27:00

Canine dna Testing, dna Extraction from Blood, What does dna Stand For

Canine dna testing is normally achieved through a small extraction of blood, hair, cheek swab or tissue sample... Tests can be done through private independent laboratories and cost from $65 up to $250 but can cost even more. The benefits can be quite amazing as we will discover later on....but first lets answer some basic questions.... What does dna stand for...? It stands for Deoxyribonucleic Acid - and is the basic foundation to all genetics. Chromosomes are made up of the Deoxyribonucleic Acid which is a double strand which divides into genes.  Your dog will have from [more...]

Date: 2009-11-23 18:25:20

Protein Synthesis, DNA Replication - DNA Station

Protein Synthesis, DNA Replication DNA replication is the process of copying a double-stranded DNA molecule to form two double-stranded molecules. The process of DNA replication is a fundamental process used by all living organisms as it is the basis for biological inheritance. As each DNA strand holds the same genetic information, both strands can serve as templates for the reproduction of the opposite strand. The template strand is preserved in its entirety and the new strand is assembled from nucleotides — this process is called “semiconservative replication”. The resulting [more...]

Date: 2010-07-27 02:56:58

Structural Biology of DNA Repair: Spatial Organisation of the ...

Nonhomologous end joining (NHEJ) plays a major role in double-strand break DNA repair, which involves a series of steps mediated by multiprotein complexes. A ring-shaped Ku70/Ku80 heterodimer forms first at broken DNA ends, [more...]

Date: 2010-07-02 07:00:00

Benitec Limited (ASX:BLT) Further Update On Successful US Patent ...

a stuffer fragment (which is a length of double stranded DNA coding for a hairpin loop in the dsRNA. Further, claim 7 describes methods for using a construct according to claim 5, and claim 10 describes methods for using a construct [more...]

Date: 2010-09-30 03:03:23

autism center of pittsburgh – Do Cell Phones Really Cause Cancer ...

Drs. Singh and Lai reported a decade ago that cell phone radiation quickly causes DNA single-strand and double-strand breaks at well below the current federal safety level for exposure. This causes a condition called micronuclei. Dr. George Carlo is a public health expert who conducted wireless technology research. He showed that human blood exposed to cell phone radiation had a 300% increase in genetic damage in the form of micronuclei. He says that wireless technology is “potentially the biggest health insult” this nation has ever seen, bigger than cigarette smoking and asbestos. Even [more...]

Date: 2010-10-13 13:00:17

Expression Profiling of PBMC-based Diagnostic Gene Markers ...

Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients Shigeto Kobayashi1, Akihiko Ito2, Daisuke Okuzaki3,4, Hiroaki Onda3,5, Norikazu Yabuta3, Ippei Nagamori3, Kazuo Suzuki6,7, Hiroshi Hashimoto1 and Hiroshi Nojima3,4,5,* 1Department of Rheumatology and Internal Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan 2Division of Molecular Pathology, Department of Cancer Biology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan 3Department of [more...]

Date: 2010-09-04 18:43:52

Science Retrospective: Who was the First to Isolate a Gene? | RNAi ...

He had the tools at his fingertips – the strains of transduced lac, a reliable exonuclease, and a method of separating and purifying each DNA strand from the lambda double helix. Nevertheless, he offered the idea to Ippen who took it back to the Beckwith lab; subsequently, she was pushed aside by the aggressive members of the group. So Ihler decided to act quickly. He grew up two transducing phages each with the lac operon in opposite orientations, he separated the DNA strands using poly-UG hybridization and density equilibrium centrifugation, and then hybridized the two opposite lambda [more...]

Date: 2010-10-06 21:59:43

Science Webulagam: Ribonucleic acid (RNA)

Ribonucleic acid (RNA) is a biologically important type of molecule that consists of a long chain of nucleotide units. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. RNA is very similar to DNA, but differs in a few important structural details: in the cell, RNA is usually single-stranded, while DNA is usually double-stranded; RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom); and RNA has the base uracil rather than thymine that is present in DNA. RNA is transcribed from DNA by enzymes called RNA [more...]

Date: 2010-04-27 13:28:00

PART 19 -- Forbidden Gates - DEFENDER PUBLISHING: PART 19 ...

Retroviruses essentially inject single-stranded rna strands into somatic (body) cells during “infection.” These ssrna strands access nucleotide pools in the host cell and form a double-stranded dna copy. This dsdna can then incorporate itself into the host chromosome using a viral enzyme called “integrase.” The new “fake gene” then orders the cell to make more mrna copies of the original virus rna. These then travel out of the cell and infect the next cell, and so on. Perhaps this type of genetic rewriting is implied in Genesis 10:8, which says, “And Cush begat Nimrod: he began [more...]

Date: 2010-10-09 14:05:00

Since the last post in Sept....

This drug is suppose to be killing the two double stranded DNA virus' that are obvious on her body. It is giving her body a head start going into the transplant. She has a transplant workshop on Tuesday. Wednesday is a colposcopy because her pap came back abnormal again. It usually reveals abnormal cells, so because of her history with this disease, it needs checked frequently to make sure these cells haven't changed into cancer. On Thursday, blood draws occur. All of these procedures can be done outpatient or on pass, so I'm checking into these options this week. It will give her a change of [more...]

Date: 2010-10-10 21:30:00


During replication of DNA, which one of the following enzymes polymerizes the Okazaki fragments? 1. DNA Polymerase I 2. DNA Polymerase II 3. DNA Polymerase III 4. RNA Polymerase I 178. During phagocytosis, the metabolic process called respiratory burst involves the activation of: 1. Oxidase 2. Hydrolase 3. Peroxidase 4. Dehydrogenase 179. Which one of the following is the complementary sequence of 5' TTAAGCTAC 3’? 1. 5’ GTACGCTTAA 3’ 2. 5’ AATTCGCATG 3’ 3. 5’ CATGCGAATT 3’ 4. 5’ TTAAGCGTAC 3’ 180. Base substitution [more...]

Date: 2010-09-02 16:44:36

MC² » Nanotechnology: Artificial Pore Created

Their paper, “Translocation of double-stranded DNA through membrane-adapted phi29 motor protein nanopores,” will appear in the journal Nature Nanotechnology, Sept. 27, 2009. The engineered channel could have applications in nano-sensing, gene delivery, drug loading and DNA sequencing,” says Guo. Guo derived the nanomotor used in the study from the biological motor of bacteriophage phi29, a virus that infects bacteria. Previously, Guo discovered that the bacteriophage phi29 DNA-packaging motor uses six molecules of the genetic material RNA to power its DNA genome through its protein [more...]

Date: 2009-09-30 16:23:59

stem cells development

Cord Blood Banking - Banking Cord Blood Banking Cord Blood - Expectant Parents Birthing Plan - Stem Cell Transplant Are aborted babies used for stem cell research? Or are they just thrown in the trash? Are there certain stages of development that are used for the research? I’ve heard that the umbilical cord can also be used. Kind of. Living human embryos are destroyed to harvest embryonic stem cells. Catholics believe that life is a sacred gift from God and needs to be treated with all human dignity from the moment of conception to the point of natural death. For this reason, the [more...]

Date: 2010-09-21 11:01:37

DNA Transcription (Advanced)

Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT The Central Dogma of Molecular Biology: “DNA makes RNA makes protein” Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place… inserting it with [more...]

Date: 2010-10-03 03:02:09

(BLOG) - Lifestyle in Computers: Exonerating the innocent: using ...

As with normal fingerprints, governments around the world have built a database of DNA fingerprints since the late 1980s. DNA often is found on the crime scene in the form of bodily fluids, such as blood. Using these samples, DNA can be compared in a laboratory using gel electrophoresis, which uses a magnetic field to separate the strands of DNA. Unfortunately, especially in the early history of DNA fingerprinting, mistakes were often made. It is estimated that one tenth of a percent of DNA comparisons are incorrectly done. This may not seem like a lot, but considering that there are more [more...]

Date: 2010-10-13 18:35:00

O.N.L.Y 1 %: 10 Tips For Better DNA Gel Extraction Results

Nabi s.a.w bersabda, maksudnya: Jabir mengatakan bahawa Rasulullah s.a.w pernah bersabda, "Wahai manusia, aku telah meninggalkan untuk kalian 2 perkara. Jika kalian menggunakannya tidak akan tersesat selamanya iaitu Al-Quran dan Ahlul Bait keturunanku." (HR At-Tarmizi dan ia menghasankannya) What is it about gel extraction of DNA that makes it a pain? Maybe it’s poor product yields or maybe it’s because the process uses harsh chemicals (chaotropic salts, ethidium bromide, ethanol, heat) that will damage or denature DNA and potentially decrease cloning success. In this article I share some [more...]

Date: 2010-10-11 04:17:00

DNA Polymerase and other factors

DNA polymerase can add free nucleotides to only the 3’ end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, therefore, needs a primer at which it can add the first nucleotide. Primers consist of RNA and DNA bases with the first two bases always being RNA, and are synthesized by another enzyme called primase. An enzyme known as a helicase is required to unwind DNA from a double-strand structure to a [more...]

Date: 2008-07-27 11:03:00

BMI1-mediated histone ubiquitylation promotes DNA double-strand ...

Ismail Hassan Ismail1,2, Christi Andrin1, Darin McDonald1, and Michael J. Hendzel1 1Department of Oncology, University of Alberta, Edmonton, T6G 1Z2 Alberta, Canada 2Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt Correspondence to Michael J. Hendzel: mhendzel{at}ualberta.ca Abstract Polycomb group (PcG) proteins are major determinants of cell identity, stem cell pluripotency, and epigenetic gene silencing during development. The polycomb repressive complex 1, which contains BMI1, RING1, and RING2, functions as an E3-ubuiquitin ligase. We found that BMI1 and [more...]

Date: 2010-10-04 15:56:59

Dr. Pandey “pulls off” new DNA sequencing method

The current method to sequence DNA is called gel-electrophoresis. This process is very costly and time consuming. However, Dr. Pandey and his research team have proposed a way to make DNA sequencing far easier and cheaper than the current method. For those of you scratching your heads and wondering what the purpose of DNA sequencing could possibly be, imagine in the futurew going to the doctor and getting customized medicine just for you based on your DNA. This medicine would do exactly what it needed to in order to fix your problem because it was made specifically for you and only you. [more...]

Date: 2010-09-23 17:36:11

The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...

There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]

Date: 2010-10-04 21:15:46

BMI1-mediated histone ubiquitylation promotes DNA double-strand ...

We found that BMI1 and RING2 are recruited to sites of DNA double-strand breaks (DSBs) where they contribute to the ubiquitylation of γ-H2AX. In the absence of BMI1, several proteins dependent on ubiquitin signaling, including 53BP1, BRCA1, and RAP80, are impaired in recruitment to DSBs. Loss of BMI1 sensitizes cells to ionizing radiation to the same extent as loss of RNF8. The simultaneous depletion of both proteins revealed an additive increase in radiation sensitivity. These data uncover an unexpected link between the polycomb and the DNA damage response pathways, and suggest a novel [more...]

Date: 2010-10-06 20:38:28

Berkeley Lab News Center Press Release: New Electrostatic-based ...

In a typical experiment, a microarray is prepared and mounted in a well chamber and the DNA is hybridized (a standard technique in which complementary single strands of DNA bind to form double-stranded DNA "hybrids"). A suspension of negatively-charged silica microspheres is then dispersed through gravitational sedimentation over the microarray surface, a process which takes about 20 minutes. Because the substrate or background surface of the microassay is positively charged, the silica microspheres will spread across the entire surface and adhere to it. However, on surface areas containing [more...]

Date: 2008-06-30 16:00:00

How the Most Influential Scientists Alive Today Unraveled the ...

DNA has been extensively researched by scientists wishing to further understand the complex way in which life is biochemically formed. DNA contains the genetic information that composes nearly all living organisms. In the early 1950s, molecular biologist James D. Watson led his team of researchers to the ground breaking discovery that DNA is a double-stranded chain, also referred to as a double helix. Using models, he showed how matching nucleotide base pairs from the two DNA strands interlocked to form a double helix. He then showed how the strands separate during replication, and how each [more...]

Date: 2010-09-23 10:31:56

$1.00 - Bio2010

DNA polymerase I catalyzes the elongation of the new DNA strands in prokaryotes. c. The lagging strand is synthesized as Okazaki fragments. d. The leading strand elongates from its 3' to 5' end. e. Helicases initiate replication by winding the two strands together. Save Answer 16. (Points: 5) The appropriate adjective to describe DNA replication is a. progressive. b. lytic. c. semiconservative. d. nondisruptive. e. natural. Save Answer 17. (Points: 5) In a sample of double-stranded DNA from a human, you have determined that 20 percent of the nitrogenous base is adenine. What percentage should [more...]

Date: 2008-08-05 01:50:23

Plant DNA Recombinases: A Long Way to Go

Mutants sensitive to DNA damaging agents like UV and ionizing radiation showed deficiency in recombination . The complex nature of the process and crosstalk between homologous recombination and other pathways like transcription, repair , ... At this stage, the newly formed double stranded DNA has the old parental strand and a new incoming strand. It is therefore called heteroduplex. These heteroduplexes further extended to mediate complete strand exchange . [more...]

Date: 2009-12-14 03:07:53

Select double-stranded DNA binding sites for transcription factors.

Can double-stranded DNA 'decoys' of transcription factors be used as drugs? How might this work? Has anyone done this?How could your data be used to find otherwise unknown transcription factor binding sites in a genome?How could your data be used to map transcriptional networks in a newly sequenced genome?How could you get data on multiple transcription factors simultaneously or in [more...]

Date: 2010-08-20 03:21:00

physics nobel prize awared to andre geim and konstantin novoselov ...

Top news :  Physics Nobel goes to graphene researchers DNA Interacts with Graphene An illustration of how fluorescent-tagged DNA interacts with functionalized graphene. Both single-stranded DNA (A) and double-stranded DNA (B) are adsorbed onto a graphene surface, but the interaction is stronger with ssDNA, causing the fluorescence on the ssDNA to darken more. C) A complimentary DNA nears the ssDNA and causes the adsorbed ssDNA to detach from the graphene surface. Original post: Grephene on Twitter Top news articlesGrephene, grephene nobel, Grephene on [more...]

Date: 2010-10-05 17:39:35

Gel electrophoresis separates DNA fragments based on their…?

Question by Nicole Has-Ellison: Gel electrophoresis separates DNA fragments based on their…? A) recognition sequence B) length C) positive charge D) chemical bonds Best answer: Answer by BP B) length but others things like salt, whether the DNA is linear or a circle, whether the DNA is single or double stranded, whether the DNA is intact or has nicks in one or both of it’s strands all affect its speed of migration in a gel… What do you think? Answer [more...]

Date: 2010-10-09 04:10:59

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