ISPUB - What's There In Our Genes? A Case For A More Inclusive And ...
We need better reviews, use of both qualitative and quantitative data as well as much more rigorous testing of newer and older models. Only then may we make full use of this large amount of data that has already been collected over the years. What we have described here, for now, presents part of the picture seen from the Indian subcontinent as an example. However, this situation is probably true of other parts of the world as well, perhaps even those areas where it has been assumed that the data is likely to be homogenous. Thus, even biological anthropologists need to add new sensitivities [more...]
Date: 2010-10-07 04:39:48
The Science: Gene Mutation
Gene Mutation In general mutation may be defined as a sudden change in the genetic material. In recent years the term mutation has generally been restricted to processes that result in a direct alteration of gene contents unrelated to observable chromosome changes. Thus, mutation at the level of nitrogenous base sequences of DNA, i.e., at the genetic level is called gene mutation or point mutation. According to strickberger, ‘point mutation or gene mutation has come to mean the process by which new alleles of gene are produced’. Gene mutation can arise spontaneously or they may be [more...]
Date: 2010-03-27 09:00:00
Antibody Therapies and the New Generation of DNA Sequencing ...
Our antibody database targets protein-coding genes. Although they form only 1% of the total human genome, they account for 85% of the mutations that lead to disease. DNA sequencing (defining the sequence of the 4 base amino acids within the DNA strand) and then using antibodies specific to those sequences, is a vital part of disease research. Sanger sequencing is the standard for doing this. However, in 2007 a next generation (or next gen) sequencing technique was devised. Lauded as the biggest advancement in genomics since microarray technology, it has radically improved diagnostic [more...]
Date: 2010-09-27 10:34:46
RNA-Seq: Comparison of sequencing-based methods to profile DNA ...
The four most frequently used sequencing-based technologies are the bisulfite-based methods MethylC-seq and reduced representation bisulfite sequencing (RRBS), and the enrichment-based techniques methylated DNA immunoprecipitation sequencing (MeDIP-seq) and methylated DNA binding domain sequencing (MBD-seq). We applied all four methods to biological replicates of human embryonic stem cells to assess their genome-wide CpG coverage, resolution, cost, concordance and the influence of CpG density and genomic context. The methylation levels assessed by the two bisulfite methods were concordant [more...]
Date: 2010-09-21 10:00:06
Scientists Discover New Repair Mechanisme For DNA Damage
“Actually DNA is highly reactive,” Nature quoted him as saying. A number of environmental toxins and chemotherapy drugs are alkylation agents that can attack DNA. When a DNA base becomes alkylated, it forms a lesion that distorts the shape of the molecule enough to prevent successful replication. Human cells contain a single glycosylase, named AAG, that repairs alkylated bases. It is specialized to detect and delete “ethenoadenine” bases, which have been deformed by combining with highly reactive, oxidized lipids in the body. However, AAG also handles many other forms of akylation [more...]
Date: 2010-10-05 07:59:00
Mother of All Humans Lived 6000 Years Ago
Further, for so few--only 21.6 nucleotides out of 16,569--DNA differences to have accumulated at anything near the measured mutation rates, a much shorter time than "200,000 years" must have transpired since Mitochondrial Eve arrived on the scene. To stretch out across evolutionary time the occurrences of so few DNA changes requires a gymnastic juggling of the coefficients used in the various models, and appears to require a biologically unrealistic, super-slow mutation rate. Making the data fit vast timescales requires the use of a broken, circular-reasoning-based, evolutionary [more...]
Date: 2010-09-07 08:00:00
Nanodevices on Track as Medical Tools | Focus
At left is a tensegrity structure made of wooden rods and string and, center, a diagrammatic image of one constructed with DNA struts (colored ladders folded into rods) and DNA cable strands (colored single lines). At right is an electron micrograph of an actual nanoscale tensegrity structure built using the new DNA-based, self-assembling nanofabrication capabilities. The scale bar equals 20 nanometers. “This new self-assembly–based nanofabrication technology could lead to nanoscale medical devices and drug delivery systems, such as virus mimics that introduce drugs directly into diseased [more...]
Date: 2010-07-30 20:45:40
Isaiah Washington- a true warrior for his people | Sierra Express ...
To many it was surprising when Sierra Leone’s President Ernest Bai Koroma decided to give citizenship to the 25 year acting veteran Isaiah Washington after he traced his ancestral roots through DNA to the Mende and Temne people of Sierra Leone. (Isaiah Washington at The Africa Policy Forum) The ‘Grey’s Anatomy’ star became the first African American to get citizenship through DNA, and President Koroma became the first African leader to award citizenship based on DNA. It is not surprising when a few days ago Africa’s most populated nation, Nigeria, decided to follow the footsteps [more...]
Date: 2010-10-07 20:00:21
20000 Genes Under the Brain: Genes->Brain->Man
To assess gene expression in each small bit of tissue, researchers expose its RNA to a gene chip, or DNA microarray. These small devices are coated with clusters of identical DNA molecules, called probes, within separate areas. Each probe binds to the RNA of a specific gene—the one that contains a complementary set of chemical units, or bases. In a DNA molecule, the base adenine (A) sticks to thymine (T), and guanine (G) pairs with cytosine (C). Thus, the sequence that would bind to the strand illustrated above is (from top to bottom): T, C, C, T, G, C, A. In this way, a chip records which [more...]
Date: 2010-09-02 16:33:00
The effect of DNA signals on nucleosome formation, chromatin ...
Much of the human genome is non-coding and it seems likely that at least some of the non-coding DNA is functionally important. However, only little is known about the possible function of the bulk of the human genome. Since essentially all the nuclear DNA is packaged into chromatin, it is likely that functional effects of non-coding DNA are mediated by the chromatin structure. By making use of human genomic sequence information now available, we showed that a specific set of periodic DNA motifs (period-10 VWG/CWB) encoded in genomic DNA is predicted to influence human chromosome function. The [more...]
Date: 2010-08-25 23:15:17
SIUC researcher part of NSF 'tree of life' project
A key aspect of the study will involve researchers from all over the world in a scientific community-based DNA sequencing project. The researchers are asking fellow scientists everywhere to send Anderson and others interesting samples of annelids for DNA sequencing. In his laboratory at SIUC, Anderson routinely does such work, turning around a sample in just a couple of days. SIUC doctoral, graduate and undergraduate students will assist in the work, gaining invaluable research experience in the process. Anderson will enter all the information into a database, which eventually will be [more...]
Date: 2010-10-06 22:10:48
Harvard-MIT study examines Graphene | UWIRE
Pore length is crucial when identifying individual DNA bases, Branton said, adding that the study “represents the first time that a molecule—DNA in our case—has been put through a nanopore that is extremely short.” Scientists in the past have studied protein-based nanopores that span five to 10 nanometers in length. But because the distance between two bases in a DNA molecule is 0.5 nanometers, such pores are ineffective, as they obstruct the resolution of 10 to 15 bases at a time. In contrast, graphene nanopores—which span a length of 0.5 nanometers—allow a single base to occupy [more...]
Date: 2010-10-05 20:48:04
Legendary Leaders and Ancestry
AfricanAncestry.com, the pioneers of DNA-based ancestry tracing for African Americans, recently revealed the paternal roots of King and Garvey through their sons — Martin Luther King III and Dr. Julius Garvey respectively – during a special DNA Reveal Dinner hosted by the Leon H. Sullivan Foundation’s African Policy Summit in Atlanta on Sept. 26. For the King Family, AfricanAncestry.com’s results complement the paper-trail research they’ve done on Dr. King’s paternal side, which traces to Ireland. Martin Luther King III’s test also revealed his mother’s line, the late [more...]
Date: 2010-10-01 14:35:47
(BLOG) - Lifestyle in Computers: Exonerating the innocent: using ...
As with normal fingerprints, governments around the world have built a database of DNA fingerprints since the late 1980s. DNA often is found on the crime scene in the form of bodily fluids, such as blood. Using these samples, DNA can be compared in a laboratory using gel electrophoresis, which uses a magnetic field to separate the strands of DNA. Unfortunately, especially in the early history of DNA fingerprinting, mistakes were often made. It is estimated that one tenth of a percent of DNA comparisons are incorrectly done. This may not seem like a lot, but considering that there are more [more...]
Date: 2010-10-13 18:35:00
Child Support and Paternity Testing » Child Support Law
Although “reasonable” expectations and common law have always been proud of having a solid blow was landed in the stomach, paternity claims common practices accepted by the scientific community that DNA tests back. Currently, a growing number of fathers are surprised by the fact that a surprising, if not more children, they believed, they are not biologically real. Surprising revelations of this kind often bring a degree of psychological trauma that many men are not prepared to handle an extremely high number of cases that alleged the man, the father discovered, in addition to all, [more...]
Date: 2010-09-04 02:24:57
BioPet DNA Breed Identification Kit | I Love Dogs Blog
Use DNA to discover the breeds present in your dog Painlessly collect your dog’s DNA sample and send it to BioPet for quick analysis Over 60 qualified breeds are on file Fast results via USPS within 2 weeks Even a prepaid return envelope for the DNA sample is included Product Description Advances in DNA testing technology mean that it has become possible to identify the ancestry of any dog by performing a simple test using a cheek swab. The DNA required for the test to be run is isolated from cells that are trapped on the DNA ID Card. All cells carry the same genetic material, regardless of [more...]
Date: 2009-12-18 14:44:21
New Repair Mechanism Has Been Discovered by Researchers « New ...
In 1953, the molecular structure of DNA was discovered by Watson and Francis Crick. Since then, one of the most amazing discoveries in genetic research has been the multitudes of protein machines that repair DNA. Recently, a “fundamentally new” DNA repair mechanism was discovered by researchers at Vanderbilt University! Science Daily describes it this way, “Tucked within its double-helix structure, DNA contains the chemical blueprint that guides all the processes that take place within the cell and are essential for life. Therefore, repairing damage and maintaining the integrity of [more...]
Date: 2010-10-13 08:16:14
Forensic Serology Information Page
What is Forensic Serology?According to forensic serologist Marcella Jones of www.ForensicMentor.com, forensic serology is the analysis of body fluids as they relate to forensic cases, including DNA analysis. Accordingly, the role of the forensic serologist involves:Examining evidence for the presence of body fluids e.g. blood, semen, hair, tissue, saliva, feces, and urineEvaluating evidence for potential DNA analysisEvaluating species of body fluidsPerform PCR (polymerase chain reaction) based typing of STR (short tandem repeat sequences) of genomic DNA, or mitochondrial sequence analysis of [more...]
Date: 2010-02-04 08:00:00
Newspaper Rock: "Actual Indian" defined
In Jocks Aren't Good Role Models, a correspondent questioned my use of the term “actual Indian.” She thought I was referring mainly to “full-blooded” Indians. No, I wasn’t—not even close. In this blog I’m usually inclusive about whom I label an Indian. The short version of who’s an “actual Indian” is “anyone whom Indians accept as an Indian.” More precisely, when Indians talk about who’s an Indian, here’s what I think they’re talking about—and therefore what I’m talking about. I’d say anyone who meets one or more of these criteria qualifies as an Indian. 1) [more...]
Date: 2008-11-07 07:05:00
DNA findings point to royal roots in African nations
Holland attributes his success to GeneTree, a Utah-based DNA testing service that came up with the Cameroon connection. But GeneTree's chief scientific officer, geneticist Scott Woodward, said Williams' case was far from unusual. "This isn't our home run," Woodward told me. "It takes a lot of regular work. But what did do was give us some nice clues and hints about where he should concentrate his efforts. Should he be looking in Cameroon, or should he be looking in Nigeria? That makes a big difference." In fact, previous genetic tests had indeed suggested that Holland's [more...]
Date: 2010-09-09 14:14:55
Engineering Mini project: DNA COMPUTERS
After seven days of intensive laboratory work, Adleman's test-tube contained the answer to the problem, subsequently visible as a series of dark bands on a DNA sequencing gel. On the face of it, it might hardly seem worth the bother, especially as Adleman already knew the answer before he started the experiment. But this was much more than a curious laboratory stunt. During the initial 'linking-up' stage of the process, Adleman's test-tube computer effectively performed an astonishing 10^14 calculations. And it did so with the consumption of only a tiny amount of energy, and in a tiny [more...]
Date: 2010-10-12 10:10:00
Thrombosis Journal | Full text | Expression of sterol regulatory ...
DNA was extracted from frozen cardiac muscle samples. The SCAP 2386A>G genotyping was based on PCR amplification, restriction enzyme analysis and DNA electrophoresis. The DNA samples were amplified by PCR, using the primers 5'-TTGTGCTGCGCGGCCACCTCA-3' and 5'-AGGAGGAAAGGGCAGCCGCAC-3'. PCR was performed in a volume of 50 μl. Cycle conditions were 94°C for 4 min, then 28 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min, with a final extension step of 5 min at 72°C in a PTC-225 thermal cycler (MJ research, Massachusetts, USA). 10% DMSO was included in PCR reaction. The [more...]
Date: 2009-02-18 08:00:00
Extra tool found in DNA's repair kit
Lesions to bases in DNA are caused by a combination of normal chemical activity within the cell and exposure to radiation and toxins coming from environmental sources including cigarette smoke, grilled foods, and industrial wastes. “Understanding protein-DNA interactions at the atomic level is important because it provides a clear starting point for designing drugs that enhance or disrupt these interactions in very specific ways,” says Brandt Eichman, associate professor of biological sciences at Vanderbilt University. “So it could lead to improved treatments for a variety of diseases, [more...]
Date: 2010-10-08 14:58:00
Have we figured out what is causing honeybee colony collapse ...
But the Army/Montana team, using a new software system developed by the military for analyzing proteins, uncovered a new DNA-based virus, and established a linkage to the fungus, called N. ceranae. The big breakthrough here was identifying the virus using the Army’s new software. In combination with the fungus, the new virus – dubbed IIV – was able to wipe out bee hives with 100% efficacy. Scientists are still not completely sure the sequence of events or even why this started happening to American honeybees and not Australian variants, for example. But this research is a major [more...]
Date: 2010-10-13 18:19:13
Xiang-Jun's Corner: Further details of the DNA story revealed by ...
I read Watson's "The Double Helix" book a while ago, and overall I am quite familiar with the DNA story. Still, I found this account fascinating: it provides a "CAST LIST" in "The search for the structure of DNA" with photos (p521); and it succinctly summarizes the relationships among the key players. In science, no other story shows more dramatically the collaborative and competitive nature among scientists working on similar projects. Franklin’s X-ray diffraction photograph 51 of B-form DNA (see figure above, from Wikipedia), with its unambiguous evidence that DNA was helical, proved [more...]
Date: 2010-10-01 03:42:00
Junk DNA and Complexity Science: A Sad mix
A quote from an extremely mediocre book, Complexity: A Guided Tour. Like most people coming out of the 'complexity sciences', the author has a mediocre grasp of molecular biology, both current developments, and the history of the field. It's frustrating that these network people keep hyping supposedly new revolutionary discoveries made the past 10 years of the genome era - discoveries, that in fact have been known for decades. For people clearly new to biology, molecular biology before the year 2000 was a big black hole of ignorance. There are several claims that make me angry in this book, [more...]
Date: 2010-05-21 19:56:10
UC Davis News & Information :: Brain Gene Shows Dramatic ...
In a computer-based search for pieces of DNA that have undergone the most change since the ancestors of humans and chimps diverged, "Human Accelerated Region 1" or HAR1, was a clear standout, said lead author Katie Pollard, assistant professor at the UC Davis Genome Center and the Department of Statistics. "It's evolving incredibly rapidly," Pollard said. "It's really an extreme case." As a postdoctoral researcher in the lab of David Haussler at UC Santa Cruz, Pollard first scanned the chimpanzee genome for stretches of DNA that were highly similar between chimpanzees, mice and rats. Then [more...]
Date: 2006-08-16 07:00:00
Exoneree Speaks About Experience With Justice System | The Cornell ...
From prison, he penned a ten-page letter to the Innocence Project –– which works to exonerate wrongly convicted prisoners based on advanced DNA testing techniques –– pleading with them to open his case. Though they agreed to take on his case in 1993, they did not have the technology available in 1996 when the DNA tests returned to officially exonerate him, as the tests were ruled inconclusive. More than a decade later, Barnes said that his brother went down to the Innocence Project and begged them to reopen Barnes' case. They agreed, and shortly thereafter –– using a [more...]
Date: 2010-10-06 04:00:00
Artwork based on DNA sequencing offers unique décor options for ...
“This ingenious service is truly personalized: the resulting artwork from an individual’s DNA will be like no other anywhere and in any time. Moreover, the fact that portraits based on DNA sequencing do not comprise human imagery will be an added attraction in the Arab world, and we can certainly expect this new kind of art form to adorn the walls of many homes here. Also, with the UAE and more specifically Abu Dhabi now promoting art and culture in a big way, as reflected by the development of five art and culture centres within its Saadiyat Island at present, DeScript will definitely [more...]
Date: 2010-09-20 03:00:38
Mitochondrial Disease can be diagnosed with a new test.
The SOLiD NextGen sequencing platform developed by Life Technologies was used for testing because of its low error rate and high throughput. This rigorous sequencing of mitochondrial DNA by MitoDx can detect mixtures (heteroplasmy) of normal and mutant DNA even when the mutant or normal form is present at very low levels. This allows mitochondrial disease to be evaluated much more sensitively in an accessible tissue like blood or saliva. Richard Boles, MD, the Director of the Mitochondrial and Metabolic Disorders Clinic at Childrens Hospital Los Angeles says, “MitoDx has confirmed the [more...]
Date: 2010-09-29 04:56:00
Distinctions Between Obesity and Waist-Hip Ratio
deCODE employs its capabilities to develop DNA-based tests and personal genome scans to better understand individual risk and empower prevention. It licenses its tests, intellectual property and analytical tools to partners, and conducts genotyping, sequencing and data analysis for companies and research institutions around the globe. Through its CLIA- and CAP-certified laboratory deCODE offers DNA-based tests for gauging risk and empowering prevention of common diseases, including deCODE Glaucoma(TM); deCODE T2(TM) for type 2 diabetes; deCODE AF(TM) for atrial fibrillation and stroke; deCODE [more...]
Date: 2010-10-11 18:15:07
DNA Diagnostics Centre UK opens the walk in DNA Testing facility
Total Articles: 106501 Total Authors: 53455 Total Downloads: 1983449 Newest Member MediaTraffic Meltdown By : Daniell Leigh Report Spam DNA Diagnostics Centre (DDC), the leading international DNA testing laboratory, recently opened a UK walk-in DNA testing facility. The facility is based in Hammersmith, West London and enables private clients, social services, law & immigration organisations to receive professional assistance on a range of DNA testing services DDC offers. DDC is recognised worldwide for its excellent service and reliable DNA test results, which are often [more...]
Date: 2010-09-06 13:00:11
DNA art imitates life: Construction of a nanoscale Mobius strip
The circulations of earth's warmer and cooler ocean currents for example, describe a Möbius shape. Other topological structures are common to biological systems, particularly in the case of DNA, the 3 billion chemical bases of which are packed by the chromosome inside the cell, using topological structures. "In bacteria, plasmid DNA is wound into a supercoil," Yan explains. "Then the enzymes can come in and cut and reconfigure the topology to relieve the torsion in the supercoil so that all the other cellular machinery can have access to the gene for replication, transcription and so [more...]
Date: 2010-10-08 21:50:00
PLANT TISSUE CULTURE TECHNIQUE AND PCR TECHNOLOGY
SSR assays require a minimal amount of genomic DNA. Most of the crop improvement programs are confined to evaluation and selection of naturally occurring clonal variations. In vitro culture techniques provide an alternative means of plant propagation and an important tool for crop improvement. The in vitro cultures are also of low risk for genetic variation since it is more resistant to genetic changes while occurrence of cell division in vitro condition. Gel electrophoresis is an important molecular biology tool which enables us to study DNA. It can be used to determine the sequence of [more...]
Date: 2010-09-08 04:07:38
Date: 2010-09-06 18:05:00
New ligands for plasmid DNA affinity purification
Molecular therapies and vaccines based on purified plasmid DNA (pDNA) are increasingly being established as promising alternatives to medical classical treatments. The expected wide application of these therapies requires the large-scale production and purification of plasmids. Recent years, had witness the development of new methods for plasmid purification, based on aqueous two-phase systems (ATPS) offering additional advantages like technical simplicity and the use of inexpensive equipment and chemicals. This project is going to study the molecular recognition mechanism and binding [more...]
Date: 2010-06-05 13:04:07
DNA Diagnostics Centre UK Opens the Walk-in DNA Testing Facility
(World Press Release) DNA Diagnostics Centre (DDC), the leading international DNA testing laboratory, recently opened a UK ‘walk-in’ DNA testing facility. The facility is based in Hammersmith, West London and enables private clients, social services, law & immigration organisations to receive professional assistance on a range of DNA testing services DDC offers. DDC is recognised worldwide for its excellent service and reliable DNA test results, which are often used to establish biological family ties in immigration cases, to resolve paternity disputes, and to provide peace of mind where [more...]
Date: 2010-09-12 10:10:12
Rivalry Among DNA Sleuths Comes Alive in Letters - 3quarksdaily
The letters were written during a 26-year period when Crick informally guided the progress of molecular biologists around the world in establishing how DNA operates in living cells. An article on the letters was published Wednesday in the journal Nature, focusing on those related to the discovery of DNA’s double helix structure in 1953. “We are really between forces that may grind all of us to pieces,” the physicist Maurice Wilkins wrote after a disastrous attempt by Crick and his colleague James D. Watson to build a model of DNA based in part on data gathered by Rosalind Franklin. [more...]
Date: 2010-10-01 11:25:46
Genetic Research: Prevention And Treatment Of Disease
The tool allows you to draw your family tree and create a chart of health history. Asking information about the illnesses that run in the family-starting from first to third generation-proves helpful. Documents that record medical transactions such as hospitalization, procedures and treatment are a good source of information. Indicate to the family tree the type of illness contracted by that family member. Because each individual has a unique genetic makeup, the reactions to certain medicines vary widely. Some patients may have more serious side effects from a certain medication, while others [more...]
Date: 2010-10-11 04:53:33
Because no one will win the debate on human evolution!
All persons have the same 100% human genes and DNA sequence-based links to an African matriarch. I refer readers to 11th January 1988 cover story in Newsweek that the DNA of humans navigate on the River. This article has been shown that evolutionists have to search, but we are afraidWrite: All people are a unique rainbow family combined. We have the saliva, the same blood type, the same shit and the same disgusting habits: we are not many, but One Race A dysfunctional family! The fact that some pre-human species changed, does not mean that all people have done things before the same thing? [more...]
Date: 2010-10-09 17:50:37
3 Ways to Lower Breast Cancer Risk, Despite Your DNA - LOVE ...
The researchers based their analysis on data from 85,644 postmenopausal women who participated in the Women's Health Initiative, a long-term study launched in 1991 and conducted by the National Institutes of Health. They considered a woman to have a family history of breast cancer if her mother or sister developed the disease after age 45.Gramling was surprised, he said, that the findings in the two groups were nearly the same."It would have been expected that women without a family history of the disease would have lowered their risk," with a healthy lifestyle, he said. That women with a [more...]
Date: 2010-10-12 19:09:17
Biosensors: Morpholino offers you more
Molecular biologists and geneticists often use DNA microarrays to detect DNA, measure gene expression levels and detect the single-nucleotide polymorphisms that are responsible for various genetic diseases. A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the [more...]
Date: 2010-10-13 09:52:22
Major breakthroughs in DNA research and the mapping of the human genome have allo scientists to learn more about what each persons strengths home testing kits and weaknesses are based on our unique DNA. Basically, it is a guessing game when we sebastian out hundreds of dollars a month on supplements. But now I know that I need to always be aware of that. home test for pregnancy Within 2 weeks of taking my custom supplements I noticed my sleeping patterns had preventing substance abuse improved dramatically. It is a fact that every human on the planet is different and their DNA proves it. The [more...]
Date: 2010-10-13 04:57:25
Dallas DNA « The Reality-Based Community
The Discovery Channel now has a documentary series based on the unit of the Dallas County DA’s office that does its own investigations of possibly innocent people in prison, rather than stonewalling demands for DNA checks as so many prosecutors’ offices do. The unit is a transparently good idea, and every major DA’s office ought to have one. If I were a Governor or a state Attorney General, I’d establish a statewide office to boot; Eric Holder might consider creating a similar unit within the Justice Department (to cover state as well as federal prisoners). The exoneration of the [more...]
Date: 2009-04-29 21:34:55
Identification of Critical Residues for the Tight Binding of Both ...
DNA polymerase Î» (Pol Î») is a novel X-family DNA polymerase that shares a 34% sequence identity with DNA polymerase Î². Pre-steady-state kinetic studies have shown that the Pol Î»-DNA complex binds both correct and incorrect nucleotides 130-fold tighter, on average, than the DNA polymerase Î²-DNA complex, although the base substitution fidelity of both polymerases is 10(-)(4) to 10(-5). To better understand Pol Î»’s tight nucleotide binding affinity, we created single-substitution and double-substitution mutants of Pol Î» to disrupt the interactions between active-site [more...]
Date: 2010-09-26 12:46:33
DNA Transcription (Advanced)
Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT The Central Dogma of Molecular Biology: “DNA makes RNA makes protein” Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place… inserting it with [more...]
Date: 2010-10-03 03:02:09
Biosensors: Morpholino offers you more
A DNA microarray has tens of thousands of biosensors on its surface; each biosensor contains a short DNA fragment, known as a probe, for recognizing DNA targets. Ideally, the DNA biosensor should have high sensitivity (the ability to detect very low concentrations of targets), high specificity (the ability to distinguish the difference between similar targets) and high stability (the ability to withstand wear and tear over many cycles). Fig. 1: Scanning electron microscopy image (left) of a cluster of parallel SiNWs, and a schematic illustration (right) of the Morpholino-based sensing [more...]
Date: 2010-10-13 12:52:44
DNA Art Blog » Blog Archive » PCR Process
We use primer sets that are optimally designed to span the specific genes you choose to have isolated for your canvas. Our technique focuses on the prevention of pre-PCR mis-priming and primer dimerization. Our primers are designed using software with algorithms that check for the potential of secondary structure formation and annealing of primers to itself or within primer pairs. We use hot start Taq Polymerase is designed to enhance the specificity, sensitivity, and yield of the target DNA sequence. Following PCR amplification and purification of the template DNA, we use restriction [more...]
Date: 2010-10-12 15:19:03
The Godless Geek Blog: Titan's Atmosphere Could Be Producing the ...
There are many theories about how exactly life began on our planet. The most commonly held belief of course, is that life began in the oceans. But a recent experiment using radio waves to simulate the effects of ultraviolet radiation from the sun striking the top of Saturn's largest moon Titan's thick atmosphere, has shown that life may actually have begun in the sky. The experiment showed that when the ultraviolet radiation strikes Titan's atmosphere it can break apart molecules in the air, like molecular nitrogen and methane, leading to the production of amino acids and the nucleotide bases [more...]
Date: 2010-10-11 02:02:00
Curious About Your DNA? Just Stop By Walgreens - Better Health
San Diego based startup Pathway Genomics announced that it will begin selling its DNA collection kits at Walgreens drugstores beginning in mid-May, for about $20 to $30. Unlike a pregnancy test, users won’t be able to get results immediately. They will have to send in their saliva sample and then go to Pathway’s website to select the particular test they want. Users choose from drug response ($79), which assesses how well an individual can metabolize certain drugs, predicting the best dosage for that person or whether they will be susceptible to certain side effects; [more...]
Date: 2010-05-20 16:00:07