Searching for cellular switches
As he “engaged science further” as an undergraduate at the University of California, San Diego, he began to appreciate how those basic atoms and molecules came together to create the building blocks of life, such as DNA, RNA and the proteins that control the body."Understanding these basic components of life made me want to further explore how biology, chemistry and physics met at a molecular interface within cells," Buchler said. He first chose to focus on physics then directed his studies more toward biology and theory as a doctoral student at the University of Michigan in Ann [more...]
Date: 2010-10-12 04:00:00
Research Progress Report I
RNA-seq utilizes high-throughput DNA sequencing method for mapping and quantifying transcriptomes (the complete set of transcripts in a cell). In this method, RNA is converted to complementary DNA fragments. Each molecule is sequenced in a high-throughput way to obtain short sequences from one or both ends. 30-400 base pairs are read, and these reads are then aligned to a reference genome, a reference transcript, or “de novo,” without the genomic sequence. This yields a genome-scale transcription map that has the transcriptional structrure and shows gene expression levels. Bead [more...]
Date: 2010-10-04 01:19:26
The Building Blocks of Evolution
Molecular evolution at the level of DNA, RNA, and proteins is precipitated by one or more of the following: - Base substitution, whereby one amino acid (nitrogenous base) is replaced by another. This is called point mutation. - Deletion of a group of bases. - Addition of a group of bases, e.g. if there are multiples of three, one or more amino acids may be deleted from the protein. - Inversion, or removal of a segment of DNA accompanied by reinsertion elsewhere but in inverted order. Related to inversion in a way is translocation, wherein a portion of DNA is attached to a homologous [more...]
Date: 2010-09-22 20:09:00
Genome Biology | Full text | Chromatin dynamics and the regulation ...
Despite these tight constraints, nucleosomes must be able to allow the DNA sequences to be accessible to DNA-binding proteins and to the action of 'molecular machines' such as DNA and RNA polymerases, ATP-dependent nucleosome .... pool by briefly labeling newly synthesized histones with a heavy isotope, such as 15N or 13C. Histone modifications can then be determined by MS for both the old and new histones, based on the mass difference between these two pools of histones. [more...]
Date: 2010-10-08 00:00:00
AquaStar Plus! UV Water Treatment Device_2060
Unsafe water іѕ placed іח tһе bottle аחԁ exposed tο UV-C light, wһісһ ԁаmаɡеѕ tһе DNA аחԁ RNA іח tһе pathogens, rendering tһеm non-infective. AquaStar Flow Through treats water іח Ɩаrɡеr batches. A small water-purification service саח generate income wһіƖе helping .... mukeh111 on The New HP Envy 17 — in 3D! materiel on Q&A: Cheap place to buy Acer Computer Aspire AS7730-4931 17 Notebook PC? rezster0900 on Q&A: What is the difference between a notebook and a laptop? [more...]
Date: 2010-10-13 09:56:06
The Genetic Code: History, Facts, and Resources About Genetics ...
TheFreeResource.com (TFR): The Genetic Code: History, Facts, and Resources About Genetics (DNA & RNA). Come be a trusted resource for someone today. ... The genetic code was discovered by Francis Crick between 1953 and 1966. He is responsible for deciphering the code that distinguish the difference between living organisms from non-living things. On a basic level, he answered the question, “What is life?” Crick did not perform this work alone however, and others including [more...]
Date: 2010-07-20 19:06:49
csirhrdg.res.in | CSIR UGC NET 2011 Life Science Syllabus 2011 ...
Analysis of RNA, DNA and proteins by one and two dimensional gel electrophoresis, Isoelectric focusing gels. Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems. Expression of recombinant proteins using bacterial, animal and plant vectors. Isolation of specific nucleic acid sequences Generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors. In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms. Protein sequencing methods, detection of post translation modification of proteins. DNA [more...]
Date: 2008-06-21 07:00:00
Bio Technology: DNA Testing (Ethnic Point of View)
Biotechnology, Mesothelioma,Gene Therapy, DNA (Deoxyribonucleic Acid), Deoxyribonucleic Acid, DNA Testing, Ethnicity in DNA Testing, Gene therapy for Asbestos, Mesothelioma Victims, DNA and RNA Structure and Function DNA testing has far reaching implications. It is being used not just for serious purposes like forensic science but also to determine the ethnicity of individuals. A particular company, marketing its tests to determine the ethnicity of people calls it recreational genomics. These tests have become extremely common these days and are being employed by a large number of [more...]
Date: 2010-08-21 00:20:00
Comments on Kathryn Applegate's May Posts on BioLogos | Uncommon ...
The DNA is located in the nucleus of a cell. When some of the information in the DNA is needed, that part of the DNA is transcribed into messenger RNA (mRNA), which carries the information into the cytoplasm of a cell. There, complex machines translate the information in the mRNA into specific proteins—antibodies or immunoglobulins are a specific type of protein. A human being’s DNA instruction book is divided into 23 chromosomes or volumes (more about this later). All DNA coding for the immunoglobulin heavy chain is found on chromosome 14: this chromosome has DNA encoding several types [more...]
Date: 2010-10-07 22:53:36
Goodbye, Nature vs Nurture « NextNature.net
Sometimes the distinction is between what is inborn and what is acquired after birth; more often, it is between genes and environment. But not only does nurture affect prenatal development, we also need to ask what exactly is a gene, and what does it do? What do we mean by environment? Does it refer to factors beyond the organism that affect its development, to the milieu in which the fertilised egg develops, or to everything other than the DNA sequence? Finally, there’s the question of contributions. Contributions to what? This question is almost never posed, yet it is the most [more...]
Date: 2010-10-09 21:31:55
Genus-Species; Doctrine of Equivalents; and Patentable Subject ...
Genus-Species; Doctrine of Equivalents; and Patentable Subject Matter By Dennis Crouch For many, the most interesting aspect of this case comes at the end in Judge Dyk’s dissent. Dyk makes the case that genes should not be patentable. * * * * Intervet Inc. v. Merial Limited (Fed. Cir. 2010) In 2006, Intervet filed a complaint against Merial — asking the DC District Court for a declaratory judgment that Intervet’s Porcine Circovirus vaccine (PCV-2) did not infringe Merial’s gene patent. Merial’s patent claims both the isolated DNA molecule of PCV-2 and a vector that contains [more...]
Date: 2010-08-05 15:54:50
Abstract | Subtype-associated differences in HIV-1 reverse ...
We analyzed the impact of the pol gene products from HIV-1 B and C subtypes on the maturation of HIV virions, accumulation of reverse transcription products, integration of viral DNA, frequency of point mutations in provirus and overall viral replication. Recombinant HIV-1 viruses of B and C subtypes comprising the pol fragments encoding protease, integrase and either the whole RT or a chimeric RT from different isolates of the C and B subtypes, were used for infection of cells expressing CXCR4 or CCR5 co-receptors. The viruses carrying different fragments of pol from the isolates of B and C [more...]
Date: 2010-10-12 00:00:00
Difference Between MRNA and TRNA | Difference Between | MRNA vs TRNA
This means that RNA has biologically programmed characteristics not found in DNA. Furthermore, RNA can be broken down into different types depending on their major functions. Among these types, the two major ones are mRNA and tRNA, having their own differences. First of all, is mRNA. It is basically a messenger RNA. Based on the word itself, the mRNA moves important information on genetic codes or “blueprints” from the DNA to its target recipient, in this case a ribosome, which functions in protein production. The mRNA is made up of long nucleotide (important molecules) chains that form [more...]
Date: 2010-06-07 00:48:04
NMCLE PART -2 NMLE QUESTION PART 2 , NMLE EXAM 2010 part -2
During replication of DNA, which one of the following enzymes polymerizes the Okazaki fragments? 1. DNA Polymerase I 2. DNA Polymerase II 3. DNA Polymerase III 4. RNA Polymerase I 178. During phagocytosis, the metabolic process called respiratory burst involves the activation of: 1. Oxidase 2. Hydrolase 3. Peroxidase 4. Dehydrogenase 179. Which one of the following is the complementary sequence of 5' TTAAGCTAC 3â? 1. 5â GTACGCTTAA 3â 2. 5â AATTCGCATG 3â 3. 5â CATGCGAATT 3â 4. 5â TTAAGCGTAC 3â 180. Base substitution [more...]
Date: 2010-09-02 16:44:36
UC Davis News & Information :: Brain Gene Shows Dramatic ...
In a computer-based search for pieces of DNA that have undergone the most change since the ancestors of humans and chimps diverged, "Human Accelerated Region 1" or HAR1, was a clear standout, said lead author Katie Pollard, assistant professor at the UC Davis Genome Center and the Department of Statistics. "It's evolving incredibly rapidly," Pollard said. "It's really an extreme case." As a postdoctoral researcher in the lab of David Haussler at UC Santa Cruz, Pollard first scanned the chimpanzee genome for stretches of DNA that were highly similar between chimpanzees, mice and rats. Then [more...]
Date: 2006-08-16 07:00:00
The MolBio Carnival, third edition | Alles was lebt | ScienceBlogs ...
There are a few methods to do this around, so why should one more on the list be interesting to us? Well, the new one uses next-generation sequencing to look at RNA folding on a whole-genome scale! David presents the paper in a journal club style, summarizing the interesting new data and methods, but also discussing some of the shortcomings. 3) Next up, we have a series of posts by Christopher Dieni at "Bitesize Bio" on the protein modification many of you probably know and love - phosphorylation. In the first post, he gives an overview of the topics he will write about in the following [more...]
Date: 2010-10-04 21:15:46
What is the different between a DNA virus and a RNA virus ...
Welcome to ScienceForums.Net! We welcome science discussion at all levels from beginners to researchers, covering topics from biology to computer science, and much more. Registration is fast and free, and allows you to post on the forums, so register now and join the discussions! After you've registered, come in and introduce yourself, or visit the forum index. If you need any help registering, posting, or if you just have some questions about our site, please feel free to contact us at staff at scienceforums dot net. Start new topics and reply to othersSubscribe to topics and forums to [more...]
Date: 2009-02-18 13:59:36
Medical Transcription From Home: What Is The Difference Between ...
DNA replication is the process by which one double helix of DNA is duplicated into two identical double helices which are also identical to the mother DNA. Translation is taking the RNA just made and using it to make a protein. Translation takes place after transcription of the genetic code into mRNA has been started. Translation refers to protein synthesis, in which proteins are made by reading the sequence information after the mature and processed mRNA by a ribosome. To me, transcription still refers to transcribing from a recording. But I thought you might want to find out why you’re [more...]
Date: 2010-08-05 18:01:00
Microsoft Research Makes Microsoft Biology Foundation and ...
MBF implements a range of parsers for common bioinformatics file formats; a range of algorithms for manipulating DNA, RNA and protein sequences; and a set of connectors to biological Web services such as National Center for Biotechnology Information BLAST.“Biologists face a number of issues today, such as detecting correlations between human genome sequencing or identifying the likelihood for a patient to develop a certain disease,” said Tony Hey, corporate vice president of Microsoft External Research. “The MBF aims to provide healthcare research facilities with the tools needed to [more...]
Date: 2010-10-13 12:28:03
Evolution News & Views: Guy Walks Into a Bar and Thinks He's a ...
Part of the reason for this is if one decides to take into account the plethora of species-specific DNA insertions and deletions ("indels") that are present along any segment compared between chimp and human, the percentage of identity drops. Another reason is that duplications, inversions, translocations, and transpositions at all scales uniquely characterize the two genome sequences -- these have to be untangled before aligning the sequences in order to measure their similarity. Also, the 99% identity figure is often derived from protein-coding regions that only comprise about 1.5% of the [more...]
Date: 2009-05-14 19:06:54